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Protein

Endoglucanase 23

Gene

At4g39000

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei410By similarity1
Active sitei470By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cell wall biogenesis/degradation, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciARA:AT4G39000-MONOMER.

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 23 (EC:3.2.1.4)
Alternative name(s):
Endo-1,4-beta glucanase 23
Gene namesi
Ordered Locus Names:At4g39000
ORF Names:F19H22.100
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G39000.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000024927524 – 493Endoglucanase 23Add BLAST470

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi297N-linked (GlcNAc...)Sequence analysis1
Glycosylationi465N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8GY58.
PRIDEiQ8GY58.

Expressioni

Gene expression databases

GenevisibleiQ8GY58. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G39000.1.

Structurei

3D structure databases

ProteinModelPortaliQ8GY58.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JTJF. Eukaryota.
ENOG41107SM. LUCA.
InParanoidiQ8GY58.
KOiK01179.
OMAiFQYADSH.
OrthoDBiEOG093608GU.
PhylomeDBiQ8GY58.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8GY58-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKASIYLVTV FILLLLLLPT AIPHDYSDAL RKSILFFEGQ RSGRLPKQQR
60 70 80 90 100
MAWRRNSALN DGKNLKTDLV GGYYDAGDNV KFHFPMAFTA TMLAWSSVDF
110 120 130 140 150
GRYMSQHDFR HNLVAVKWAT DYLLKTVSQL PNRIFVHVGE VQPDHDCWER
160 170 180 190 200
PEDMDTPRTA FALDAPYPAS DLAGEIAAAL AAASIAFKQA NPKYSAILLN
210 220 230 240 250
KAVQTFQYAD SHRGSYTDNP GIKQAVCPFY CSVNGYKDEL LWGAAWLRRA
260 270 280 290 300
TGEDSYLRYL VDNGQAFGES SNYFEFGWDN KVGGVNVLVA KEVLQNNVTA
310 320 330 340 350
IAAYKDTAEK MMCSFLPETN GPHMSYTPGG LIYKPGSTQL QNTAALSFLL
360 370 380 390 400
LTYADYLSTS SQQLNCGNLK FQPDSLRRIV KRQVDYVLGD NPMKLSYMIG
410 420 430 440 450
YGERYPGLIH HRGSSIPSVT VHPAAFGCIA GWNIFSSPNP NPNILIGAVI
460 470 480 490
GGPDVDDRFI GGRTNASETE PTTYINAPFV GVFAYFKSNP NFS
Length:493
Mass (Da):54,692
Last modified:September 5, 2006 - v2
Checksum:iB999CEC76F5FDFC3
GO
Isoform 2 (identifier: Q8GY58-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     292-310: EVLQNNVTAIAAYKDTAEK → VWSNFQNQTDVYIYDKCDR
     311-493: Missing.

Note: No experimental confirmation available.
Show »
Length:310
Mass (Da):35,065
Checksum:iBDC4CF88CD8C2535
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti97Missing in BAC42491 (PubMed:11910074).Curated1
Sequence conflicti215S → T in BAC42491 (PubMed:11910074).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020387292 – 310EVLQN…DTAEK → VWSNFQNQTDVYIYDKCDR in isoform 2. 1 PublicationAdd BLAST19
Alternative sequenceiVSP_020388311 – 493Missing in isoform 2. 1 PublicationAdd BLAST183

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL035679 Genomic DNA. Translation: CAB38820.1.
AL161594 Genomic DNA. Translation: CAB80563.1.
CP002687 Genomic DNA. Translation: AEE87006.1.
AK117850 mRNA. Translation: BAC42491.1.
DQ446906 mRNA. Translation: ABE66120.1.
PIRiT06060.
RefSeqiNP_195611.1. NM_120060.2. [Q8GY58-1]
UniGeneiAt.31125.

Genome annotation databases

EnsemblPlantsiAT4G39000.1; AT4G39000.1; AT4G39000. [Q8GY58-1]
GeneIDi830055.
GrameneiAT4G39000.1; AT4G39000.1; AT4G39000.
KEGGiath:AT4G39000.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL035679 Genomic DNA. Translation: CAB38820.1.
AL161594 Genomic DNA. Translation: CAB80563.1.
CP002687 Genomic DNA. Translation: AEE87006.1.
AK117850 mRNA. Translation: BAC42491.1.
DQ446906 mRNA. Translation: ABE66120.1.
PIRiT06060.
RefSeqiNP_195611.1. NM_120060.2. [Q8GY58-1]
UniGeneiAt.31125.

3D structure databases

ProteinModelPortaliQ8GY58.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G39000.1.

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Proteomic databases

PaxDbiQ8GY58.
PRIDEiQ8GY58.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G39000.1; AT4G39000.1; AT4G39000. [Q8GY58-1]
GeneIDi830055.
GrameneiAT4G39000.1; AT4G39000.1; AT4G39000.
KEGGiath:AT4G39000.

Organism-specific databases

TAIRiAT4G39000.

Phylogenomic databases

eggNOGiENOG410JTJF. Eukaryota.
ENOG41107SM. LUCA.
InParanoidiQ8GY58.
KOiK01179.
OMAiFQYADSH.
OrthoDBiEOG093608GU.
PhylomeDBiQ8GY58.

Enzyme and pathway databases

BioCyciARA:AT4G39000-MONOMER.

Miscellaneous databases

PROiQ8GY58.

Gene expression databases

GenevisibleiQ8GY58. AT.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGUN23_ARATH
AccessioniPrimary (citable) accession number: Q8GY58
Secondary accession number(s): Q9SVJ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 5, 2006
Last modified: November 30, 2016
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

The conserved 'Asp-461' active site is replaced by a Gly residue.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.