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Protein

Endoglucanase 23

Gene

At4g39000

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Caution

The conserved 'Asp-461' active site is replaced by a Gly residue.Curated

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei410By similarity1
Active sitei470By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cell wall biogenesis/degradation, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciARA:AT4G39000-MONOMER

Protein family/group databases

CAZyiGH9 Glycoside Hydrolase Family 9

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 23 (EC:3.2.1.4)
Alternative name(s):
Endo-1,4-beta glucanase 23
Gene namesi
Ordered Locus Names:At4g39000
ORF Names:F19H22.100
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G39000
TAIRilocus:2120232 AT4G39000

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000024927524 – 493Endoglucanase 23Add BLAST470

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi297N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi465N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8GY58

Expressioni

Gene expression databases

ExpressionAtlasiQ8GY58 differential
GenevisibleiQ8GY58 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G39000.1

Structurei

3D structure databases

ProteinModelPortaliQ8GY58
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JTJF Eukaryota
ENOG41107SM LUCA
InParanoidiQ8GY58
KOiK01179
OMAiETNGPHM
OrthoDBiEOG093608GU
PhylomeDBiQ8GY58

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR008928 6-hairpin_glycosidase_sf
IPR012341 6hp_glycosidase-like_sf
IPR001701 Glyco_hydro_9
PfamiView protein in Pfam
PF00759 Glyco_hydro_9, 1 hit
SUPFAMiSSF48208 SSF48208, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8GY58-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKASIYLVTV FILLLLLLPT AIPHDYSDAL RKSILFFEGQ RSGRLPKQQR
60 70 80 90 100
MAWRRNSALN DGKNLKTDLV GGYYDAGDNV KFHFPMAFTA TMLAWSSVDF
110 120 130 140 150
GRYMSQHDFR HNLVAVKWAT DYLLKTVSQL PNRIFVHVGE VQPDHDCWER
160 170 180 190 200
PEDMDTPRTA FALDAPYPAS DLAGEIAAAL AAASIAFKQA NPKYSAILLN
210 220 230 240 250
KAVQTFQYAD SHRGSYTDNP GIKQAVCPFY CSVNGYKDEL LWGAAWLRRA
260 270 280 290 300
TGEDSYLRYL VDNGQAFGES SNYFEFGWDN KVGGVNVLVA KEVLQNNVTA
310 320 330 340 350
IAAYKDTAEK MMCSFLPETN GPHMSYTPGG LIYKPGSTQL QNTAALSFLL
360 370 380 390 400
LTYADYLSTS SQQLNCGNLK FQPDSLRRIV KRQVDYVLGD NPMKLSYMIG
410 420 430 440 450
YGERYPGLIH HRGSSIPSVT VHPAAFGCIA GWNIFSSPNP NPNILIGAVI
460 470 480 490
GGPDVDDRFI GGRTNASETE PTTYINAPFV GVFAYFKSNP NFS
Length:493
Mass (Da):54,692
Last modified:September 5, 2006 - v2
Checksum:iB999CEC76F5FDFC3
GO
Isoform 2 (identifier: Q8GY58-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     292-310: EVLQNNVTAIAAYKDTAEK → VWSNFQNQTDVYIYDKCDR
     311-493: Missing.

Note: No experimental confirmation available.
Show »
Length:310
Mass (Da):35,065
Checksum:iBDC4CF88CD8C2535
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti97Missing in BAC42491 (PubMed:11910074).Curated1
Sequence conflicti215S → T in BAC42491 (PubMed:11910074).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020387292 – 310EVLQN…DTAEK → VWSNFQNQTDVYIYDKCDR in isoform 2. 1 PublicationAdd BLAST19
Alternative sequenceiVSP_020388311 – 493Missing in isoform 2. 1 PublicationAdd BLAST183

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL035679 Genomic DNA Translation: CAB38820.1
AL161594 Genomic DNA Translation: CAB80563.1
CP002687 Genomic DNA Translation: AEE87006.1
AK117850 mRNA Translation: BAC42491.1
DQ446906 mRNA Translation: ABE66120.1
PIRiT06060
RefSeqiNP_195611.1, NM_120060.2 [Q8GY58-1]
UniGeneiAt.31125

Genome annotation databases

EnsemblPlantsiAT4G39000.1; AT4G39000.1; AT4G39000 [Q8GY58-1]
GeneIDi830055
GrameneiAT4G39000.1; AT4G39000.1; AT4G39000 [Q8GY58-1]
KEGGiath:AT4G39000

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGUN23_ARATH
AccessioniPrimary (citable) accession number: Q8GY58
Secondary accession number(s): Q9SVJ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 5, 2006
Last modified: May 23, 2018
This is version 98 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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