Q8GY58 (GUN23_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase 23
EC=3.2.1.4

Alternative name(s):
Endo-1,4-beta glucanase 23

Gene names

Ordered Locus Names:	At4g39000
ORF Names:	F19H22.100

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.
Caution	The conserved 'Asp-461' active site is replaced by a Gly residue.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8GY58-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8GY58-2)

The sequence of this isoform differs from the canonical sequence as follows:
     292-310: EVLQNNVTAIAAYKDTAEK → VWSNFQNQTDVYIYDKCDR
     311-493: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 493	470	Endoglucanase 23		PRO_0000249275
Sites
	Active site	410	1	By similarity
	Active site	470	1	By similarity
Amino acid modifications
	Glycosylation	297	1	N-linked (GlcNAc...) Potential
	Glycosylation	465	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	292 – 310	19	EVLQN…DTAEK → VWSNFQNQTDVYIYDKCDR in isoform 2.		VSP_020387
	Alternative sequence	311 – 493	183	Missing in isoform 2.		VSP_020388
Experimental info
	Sequence conflict	97	1	Missing in BAC42491. Ref.3
	Sequence conflict	215	1	S → T in BAC42491. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 5, 2006. Version 2. Checksum: B999CEC76F5FDFC3	FASTA	493	54,692
	10 20 30 40 50 60 MKASIYLVTV FILLLLLLPT AIPHDYSDAL RKSILFFEGQ RSGRLPKQQR MAWRRNSALN 70 80 90 100 110 120 DGKNLKTDLV GGYYDAGDNV KFHFPMAFTA TMLAWSSVDF GRYMSQHDFR HNLVAVKWAT 130 140 150 160 170 180 DYLLKTVSQL PNRIFVHVGE VQPDHDCWER PEDMDTPRTA FALDAPYPAS DLAGEIAAAL 190 200 210 220 230 240 AAASIAFKQA NPKYSAILLN KAVQTFQYAD SHRGSYTDNP GIKQAVCPFY CSVNGYKDEL 250 260 270 280 290 300 LWGAAWLRRA TGEDSYLRYL VDNGQAFGES SNYFEFGWDN KVGGVNVLVA KEVLQNNVTA 310 320 330 340 350 360 IAAYKDTAEK MMCSFLPETN GPHMSYTPGG LIYKPGSTQL QNTAALSFLL LTYADYLSTS 370 380 390 400 410 420 SQQLNCGNLK FQPDSLRRIV KRQVDYVLGD NPMKLSYMIG YGERYPGLIH HRGSSIPSVT 430 440 450 460 470 480 VHPAAFGCIA GWNIFSSPNP NPNILIGAVI GGPDVDDRFI GGRTNASETE PTTYINAPFV 490 GVFAYFKSNP NFS
	Isoform 2 [UniParc]. Checksum: BDC4CF88CD8C2535	FASTA	310	35,065
	10 20 30 40 50 60 MKASIYLVTV FILLLLLLPT AIPHDYSDAL RKSILFFEGQ RSGRLPKQQR MAWRRNSALN 70 80 90 100 110 120 DGKNLKTDLV GGYYDAGDNV KFHFPMAFTA TMLAWSSVDF GRYMSQHDFR HNLVAVKWAT 130 140 150 160 170 180 DYLLKTVSQL PNRIFVHVGE VQPDHDCWER PEDMDTPRTA FALDAPYPAS DLAGEIAAAL 190 200 210 220 230 240 AAASIAFKQA NPKYSAILLN KAVQTFQYAD SHRGSYTDNP GIKQAVCPFY CSVNGYKDEL 250 260 270 280 290 300 LWGAAWLRRA TGEDSYLRYL VDNGQAFGES SNYFEFGWDN KVGGVNVLVA KVWSNFQNQT 310 DVYIYDKCDR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[4]	"Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations." Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P. Plant Biotechnol. J. 4:317-324(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[5]	"Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family." Libertini E., Li Y., McQueen-Mason S.J. J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL035679 Genomic DNA. Translation: CAB38820.1. AL161594 Genomic DNA. Translation: CAB80563.1. CP002687 Genomic DNA. Translation: AEE87006.1. AK117850 mRNA. Translation: BAC42491.1. DQ446906 mRNA. Translation: ABE66120.1.
IPI	IPI00519148. IPI00785814.
PIR	T06060.
RefSeq	NP_195611.1. NM_120060.1.
UniGene	At.31125.
3D structure databases
HSSP	HSSP built from PDB template 1TF4 based on UniProtKB P26221.
ProteinModelPortal	Q8GY58.
SMR	Q8GY58. Positions 25-484.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT4G39000.1-P.
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Proteomic databases
PRIDE	Q8GY58.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G39000.1; AT4G39000.1; AT4G39000.
GeneID	830055.
KEGG	ath:AT4G39000.
Organism-specific databases
TAIR	At4g39000.
Phylogenomic databases
eggNOG	NOG322234.
InParanoid	Q8GY58.
KO	K01179.
OMA	IAFKQAN.
PhylomeDB	Q8GY58.
ProtClustDB	CLSN2915965.
Gene expression databases
ArrayExpress	Q8GY58.
Genevestigator	Q8GY58.
GermOnline	AT4G39000. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. [Graphical view]
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. False negative. PS00698. GLYCOSYL_HYDROL_F9_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN23_ARATH

Accession

Primary (citable) accession number: Q8GY58
Secondary accession number(s): Q9SVJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	September 5, 2006
Last modified:	May 1, 2013
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program