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Q8GY58

- GUN23_ARATH

UniProt

Q8GY58 - GUN23_ARATH

Protein

Endoglucanase 23

Gene

At4g39000

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 2 (05 Sep 2006)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei410 – 4101By similarity
    Active sitei470 – 4701By similarity

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cell wall biogenesis/degradation, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciARA:AT4G39000-MONOMER.

    Protein family/group databases

    CAZyiGH9. Glycoside Hydrolase Family 9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase 23 (EC:3.2.1.4)
    Alternative name(s):
    Endo-1,4-beta glucanase 23
    Gene namesi
    Ordered Locus Names:At4g39000
    ORF Names:F19H22.100
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G39000.

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 493470Endoglucanase 23PRO_0000249275Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi465 – 4651N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ8GY58.

    Expressioni

    Gene expression databases

    GenevestigatoriQ8GY58.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT4G39000.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8GY58.
    SMRiQ8GY58. Positions 25-484.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG322234.
    InParanoidiQ8GY58.
    KOiK01179.
    OMAiFQYADSH.
    PhylomeDBiQ8GY58.

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR001701. Glyco_hydro_9.
    [Graphical view]
    PfamiPF00759. Glyco_hydro_9. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8GY58-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKASIYLVTV FILLLLLLPT AIPHDYSDAL RKSILFFEGQ RSGRLPKQQR    50
    MAWRRNSALN DGKNLKTDLV GGYYDAGDNV KFHFPMAFTA TMLAWSSVDF 100
    GRYMSQHDFR HNLVAVKWAT DYLLKTVSQL PNRIFVHVGE VQPDHDCWER 150
    PEDMDTPRTA FALDAPYPAS DLAGEIAAAL AAASIAFKQA NPKYSAILLN 200
    KAVQTFQYAD SHRGSYTDNP GIKQAVCPFY CSVNGYKDEL LWGAAWLRRA 250
    TGEDSYLRYL VDNGQAFGES SNYFEFGWDN KVGGVNVLVA KEVLQNNVTA 300
    IAAYKDTAEK MMCSFLPETN GPHMSYTPGG LIYKPGSTQL QNTAALSFLL 350
    LTYADYLSTS SQQLNCGNLK FQPDSLRRIV KRQVDYVLGD NPMKLSYMIG 400
    YGERYPGLIH HRGSSIPSVT VHPAAFGCIA GWNIFSSPNP NPNILIGAVI 450
    GGPDVDDRFI GGRTNASETE PTTYINAPFV GVFAYFKSNP NFS 493
    Length:493
    Mass (Da):54,692
    Last modified:September 5, 2006 - v2
    Checksum:iB999CEC76F5FDFC3
    GO
    Isoform 2 (identifier: Q8GY58-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         292-310: EVLQNNVTAIAAYKDTAEK → VWSNFQNQTDVYIYDKCDR
         311-493: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:310
    Mass (Da):35,065
    Checksum:iBDC4CF88CD8C2535
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti97 – 971Missing in BAC42491. (PubMed:11910074)Curated
    Sequence conflicti215 – 2151S → T in BAC42491. (PubMed:11910074)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei292 – 31019EVLQN…DTAEK → VWSNFQNQTDVYIYDKCDR in isoform 2. 1 PublicationVSP_020387Add
    BLAST
    Alternative sequencei311 – 493183Missing in isoform 2. 1 PublicationVSP_020388Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL035679 Genomic DNA. Translation: CAB38820.1.
    AL161594 Genomic DNA. Translation: CAB80563.1.
    CP002687 Genomic DNA. Translation: AEE87006.1.
    AK117850 mRNA. Translation: BAC42491.1.
    DQ446906 mRNA. Translation: ABE66120.1.
    PIRiT06060.
    RefSeqiNP_195611.1. NM_120060.1. [Q8GY58-1]
    UniGeneiAt.31125.

    Genome annotation databases

    EnsemblPlantsiAT4G39000.1; AT4G39000.1; AT4G39000. [Q8GY58-1]
    GeneIDi830055.
    KEGGiath:AT4G39000.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL035679 Genomic DNA. Translation: CAB38820.1 .
    AL161594 Genomic DNA. Translation: CAB80563.1 .
    CP002687 Genomic DNA. Translation: AEE87006.1 .
    AK117850 mRNA. Translation: BAC42491.1 .
    DQ446906 mRNA. Translation: ABE66120.1 .
    PIRi T06060.
    RefSeqi NP_195611.1. NM_120060.1. [Q8GY58-1 ]
    UniGenei At.31125.

    3D structure databases

    ProteinModelPortali Q8GY58.
    SMRi Q8GY58. Positions 25-484.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT4G39000.1-P.

    Protein family/group databases

    CAZyi GH9. Glycoside Hydrolase Family 9.

    Proteomic databases

    PRIDEi Q8GY58.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G39000.1 ; AT4G39000.1 ; AT4G39000 . [Q8GY58-1 ]
    GeneIDi 830055.
    KEGGi ath:AT4G39000.

    Organism-specific databases

    TAIRi AT4G39000.

    Phylogenomic databases

    eggNOGi NOG322234.
    InParanoidi Q8GY58.
    KOi K01179.
    OMAi FQYADSH.
    PhylomeDBi Q8GY58.

    Enzyme and pathway databases

    BioCyci ARA:AT4G39000-MONOMER.

    Gene expression databases

    Genevestigatori Q8GY58.

    Family and domain databases

    Gene3Di 1.50.10.10. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR001701. Glyco_hydro_9.
    [Graphical view ]
    Pfami PF00759. Glyco_hydro_9. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48208. SSF48208. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Columbia.
    4. "Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations."
      Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.
      Plant Biotechnol. J. 4:317-324(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    5. "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family."
      Libertini E., Li Y., McQueen-Mason S.J.
      J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY.

    Entry informationi

    Entry nameiGUN23_ARATH
    AccessioniPrimary (citable) accession number: Q8GY58
    Secondary accession number(s): Q9SVJ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 77 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3