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Protein

Dual specificity phosphatase Cdc25

Gene

CDC25

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Tyrosine protein phosphatase that dephosphorylates CDK complex and activate its kinase activity in vitro.
Arsenate reductase that plays a major role in the reduction of arsenate to arsenite and arsenic retention in roots.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 Publication

Enzyme regulationi

Inhibited by NSC95397.1 Publication

Kineticsi

  1. KM=50 mM for para-nitrophenyl phosphate2 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi53Zinc2 Publications1
    Active sitei86Phosphocysteine intermediateCurated1
    Metal bindingi134Zinc2 Publications1
    Metal bindingi136Zinc2 Publications1
    Metal bindingi141Zinc2 Publications1

    GO - Molecular functioni

    • metal ion binding Source: UniProtKB-KW
    • oxidoreductase activity Source: UniProtKB-KW
    • protein tyrosine phosphatase activity Source: TAIR

    GO - Biological processi

    • cell division Source: UniProtKB-KW
    • mitotic nuclear division Source: UniProtKB-KW
    • protein phosphorylation Source: TAIR
    • response to arsenic-containing substance Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Oxidoreductase, Protein phosphatase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciARA:AT5G03455-MONOMER.
    SABIO-RKQ8GY31.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dual specificity phosphatase Cdc25 (EC:3.1.3.48)
    Alternative name(s):
    Arath;CDC25
    Arsenate reductase 2 (EC:1.20.4.-)
    Sulfurtransferase 5
    Short name:
    AtStr5
    Gene namesi
    Name:CDC25
    Synonyms:ACR2, STR5
    Ordered Locus Names:At5g03455
    ORF Names:F12E4_220
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    TAIRiAT5G03455.

    Subcellular locationi

    GO - Cellular componenti

    • chloroplast Source: TAIR
    • mitochondrion Source: TAIR
    • nucleus Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype under normal growth conditions, but plants show reduced root size when grown in presence of hydroxyurea.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi86C → S: Loss of phosphatase activity. 1 Publication1
    Mutagenesisi145C → S: No major structural changes. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001986631 – 146Dual specificity phosphatase Cdc25Add BLAST146

    Proteomic databases

    PaxDbiQ8GY31.

    Expressioni

    Tissue specificityi

    Expressed in roots and at lower levels in shoots (at protein level). Expressed in leaves, stems and flowers.2 Publications

    Gene expression databases

    GenevisibleiQ8GY31. AT.

    Interactioni

    Protein-protein interaction databases

    BioGridi17108. 3 interactors.
    IntActiQ8GY31. 3 interactors.
    STRINGi3702.AT5G03455.1.

    Structurei

    Secondary structure

    1146
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi19 – 24Combined sources6
    Turni26 – 31Combined sources6
    Beta strandi38 – 44Combined sources7
    Helixi47 – 50Combined sources4
    Beta strandi56 – 60Combined sources5
    Beta strandi63 – 66Combined sources4
    Helixi69 – 74Combined sources6
    Beta strandi81 – 87Combined sources7
    Beta strandi90 – 92Combined sources3
    Helixi93 – 106Combined sources14
    Beta strandi107 – 109Combined sources3
    Beta strandi113 – 121Combined sources9
    Helixi124 – 129Combined sources6

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1T3KNMR-A15-146[»]
    ProteinModelPortaliQ8GY31.
    SMRiQ8GY31.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8GY31.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini34 – 135RhodanesePROSITE-ProRule annotationAdd BLAST102

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni45 – 48Substrate-binding4
    Regioni68 – 71Substrate-binding4
    Regioni90 – 92Substrate-binding3

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi11 – 14Poly-Lys4

    Sequence similaritiesi

    Belongs to the MPI phosphatase family.Curated
    Contains 1 rhodanese domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiENOG410IQGW. Eukaryota.
    ENOG4111VH0. LUCA.
    HOGENOMiHOG000162894.
    InParanoidiQ8GY31.
    KOiK18065.
    OMAiNIMILER.
    OrthoDBiEOG09360QPX.
    PhylomeDBiQ8GY31.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    InterProiIPR001763. Rhodanese-like_dom.
    [Graphical view]
    PfamiPF00581. Rhodanese. 1 hit.
    [Graphical view]
    SMARTiSM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8GY31-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGRSIFSFFT KKKKMAMARS ISYITSTQLL PLHRRPNIAI IDVRDEERNY
    60 70 80 90 100
    DGHIAGSLHY ASGSFDDKIS HLVQNVKDKD TLVFHCALSQ VRGPTCARRL
    110 120 130 140
    VNYLDEKKED TGIKNIMILE RGFNGWEASG KPVCRCAEVP CKGDCA
    Length:146
    Mass (Da):16,450
    Last modified:March 1, 2003 - v1
    Checksum:iEA2DD0E79784D538
    GO

    Sequence cautioni

    The sequence AAM63780 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
    The sequence CAB83305 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL162751 Genomic DNA. Translation: CAB83305.1. Sequence problems.
    CP002688 Genomic DNA. Translation: AED90607.1.
    AK117898 mRNA. Translation: BAC42537.1.
    BT003658 mRNA. Translation: AAO39886.1.
    AY086729 mRNA. Translation: AAM63780.1. Different initiation.
    PIRiT48370.
    RefSeqiNP_568119.1. NM_120425.3.
    UniGeneiAt.43043.

    Genome annotation databases

    EnsemblPlantsiAT5G03455.1; AT5G03455.1; AT5G03455.
    GeneIDi831832.
    GrameneiAT5G03455.1; AT5G03455.1; AT5G03455.
    KEGGiath:AT5G03455.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL162751 Genomic DNA. Translation: CAB83305.1. Sequence problems.
    CP002688 Genomic DNA. Translation: AED90607.1.
    AK117898 mRNA. Translation: BAC42537.1.
    BT003658 mRNA. Translation: AAO39886.1.
    AY086729 mRNA. Translation: AAM63780.1. Different initiation.
    PIRiT48370.
    RefSeqiNP_568119.1. NM_120425.3.
    UniGeneiAt.43043.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1T3KNMR-A15-146[»]
    ProteinModelPortaliQ8GY31.
    SMRiQ8GY31.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi17108. 3 interactors.
    IntActiQ8GY31. 3 interactors.
    STRINGi3702.AT5G03455.1.

    Proteomic databases

    PaxDbiQ8GY31.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT5G03455.1; AT5G03455.1; AT5G03455.
    GeneIDi831832.
    GrameneiAT5G03455.1; AT5G03455.1; AT5G03455.
    KEGGiath:AT5G03455.

    Organism-specific databases

    TAIRiAT5G03455.

    Phylogenomic databases

    eggNOGiENOG410IQGW. Eukaryota.
    ENOG4111VH0. LUCA.
    HOGENOMiHOG000162894.
    InParanoidiQ8GY31.
    KOiK18065.
    OMAiNIMILER.
    OrthoDBiEOG09360QPX.
    PhylomeDBiQ8GY31.

    Enzyme and pathway databases

    BioCyciARA:AT5G03455-MONOMER.
    SABIO-RKQ8GY31.

    Miscellaneous databases

    EvolutionaryTraceiQ8GY31.
    PROiQ8GY31.

    Gene expression databases

    GenevisibleiQ8GY31. AT.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    InterProiIPR001763. Rhodanese-like_dom.
    [Graphical view]
    PfamiPF00581. Rhodanese. 1 hit.
    [Graphical view]
    SMARTiSM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCDC25_ARATH
    AccessioniPrimary (citable) accession number: Q8GY31
    Secondary accession number(s): Q8LC90, Q9LZE1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: March 1, 2003
    Last modified: November 30, 2016
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Binds 1 zinc ion which is not required for enzyme activity (PubMed:15329414). Plants silencing ACR2 show increased sensitivity to arsenate but not arsenite (PubMed:16567632).2 Publications

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.