Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

E3 ubiquitin-protein ligase UPL1

Gene

UPL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins.1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3648Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ubiquitin protein ligase activity Source: GO_Central
  • ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  • protein ubiquitination Source: TAIR

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-ATH-6798695 Neutrophil degranulation
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase UPL1 (EC:2.3.2.26)
Short name:
Ubiquitin-protein ligase 1
Alternative name(s):
HECT-type E3 ubiquitin transferase UPL1
Gene namesi
Name:UPL1
Ordered Locus Names:At1g55860
ORF Names:F14J16.14, F14J16.37
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G55860
TAIRilocus:2012060 AT1G55860

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi3648C → S or A: Abolishes ability to conjugate ubiquitin in vitro. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001203431 – 3681E3 ubiquitin-protein ligase UPL1Add BLAST3681

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2598PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8GY23
PRIDEiQ8GY23

PTM databases

iPTMnetiQ8GY23

Expressioni

Tissue specificityi

Widely expressed. Expressed in root, stem, cauline and rosette leaf, seedling and flower (at protein level).1 Publication

Developmental stagei

Constitutively expressed throughout development post-germination (at protein level).1 Publication

Gene expression databases

ExpressionAtlasiQ8GY23 baseline and differential
GenevisibleiQ8GY23 AT

Interactioni

Protein-protein interaction databases

BioGridi272611 interactor.
STRINGi3702.AT1G55860.1

Structurei

3D structure databases

ProteinModelPortaliQ8GY23
SMRiQ8GY23
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1269 – 1310UBAPROSITE-ProRule annotationAdd BLAST42
Domaini1316 – 1335UIMPROSITE-ProRule annotationAdd BLAST20
Domaini3340 – 3681HECTPROSITE-ProRule annotationAdd BLAST342

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi2129 – 2190Asp-richAdd BLAST62

Sequence similaritiesi

Belongs to the UPL family. TOM1/PTR1 subfamily.Curated

Phylogenomic databases

eggNOGiKOG0939 Eukaryota
COG5021 LUCA
HOGENOMiHOG000084686
InParanoidiQ8GY23
KOiK10592
OrthoDBiEOG0936000C
PhylomeDBiQ8GY23

Family and domain databases

CDDicd00078 HECTc, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR025527 DUF4414
IPR010309 E3_Ub_ligase_DUF908
IPR010314 E3_Ub_ligase_DUF913
IPR000569 HECT_dom
IPR035983 Hect_E3_ubiquitin_ligase
IPR015940 UBA
IPR009060 UBA-like_sf
IPR003903 UIM_dom
PfamiView protein in Pfam
PF14377 DUF4414, 1 hit
PF06012 DUF908, 2 hits
PF06025 DUF913, 1 hit
PF00632 HECT, 1 hit
PF00627 UBA, 1 hit
SMARTiView protein in SMART
SM00119 HECTc, 1 hit
SM00165 UBA, 1 hit
SUPFAMiSSF46934 SSF46934, 1 hit
SSF48371 SSF48371, 11 hits
SSF56204 SSF56204, 1 hit
PROSITEiView protein in PROSITE
PS50237 HECT, 1 hit
PS50030 UBA, 1 hit
PS50330 UIM, 1 hit

Sequencei

Sequence statusi: Complete.

Q8GY23-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLRRRRASE VPSKIKSFIN SVTSVPLELI HEPLACFRWE FDKGDFHHWV
60 70 80 90 100
DLFNYFDTFF EKHVQVRKDL HIEENFEESD PPFPKDAVLQ VLRVIRVVLE
110 120 130 140 150
NCTNKHFYSS YEQHLSLLLA STDADVVEAC LQTLAAFLKR QIGKYSIRDA
160 170 180 190 200
SLNSKLFSLA QGWGGKEEGL GLTSCAAENS CDQVSLQLGR TLHFEFYPSD
210 220 230 240 250
ESPSELPGGL QVIHVPDVSI CAESDLELLN KLVIDHNVPP SLRFALLTRM
260 270 280 290 300
RFARAFSSLA TRQQFTCIRL YAFVVLVQAS GDTENVVSFF NGEPEFVNEL
310 320 330 340 350
VTLVSYEDTV PEKIRILCLL SLVALSQDRT RQPTVLTAVT SGGHRGLLSG
360 370 380 390 400
LMQKAIDSVV CITSKWSLAF AEALLSLVTV LVSSSSGCSA MREAGLIPTL
410 420 430 440 450
VPLIKDTDPQ HLHLVSAAVH ILEAFMDYSN PAAALFRDLG GLDDTIFRLK
460 470 480 490 500
LEVSRTEDDV KEKNCSSDSN GPDTEQLPYS EALISYHRRL LLKALLRAIS
510 520 530 540 550
LGTYAPGNTN LYGSEESLLP ECLCIIFRRA KDFGGGVFSL AATVMSDLIH
560 570 580 590 600
KDPTCFNALD SAGLTSTFLD AISDEVICSA EAITCIPQCL DALCLNNSGL
610 620 630 640 650
QAVKDRNALR CFVKIFTSPS YLRALTGDTP GSLSSGLDEL LRHQSSLRTY
660 670 680 690 700
GVDMFIEILN SMLIIGSGME ATTSKSADVP TSAAPVPMEI DVDEKSLAVS
710 720 730 740 750
DEAEPSSDTS PANIELFLPD CVCNVARLFE TVLQNAEVCS LFVEKKGIDA
760 770 780 790 800
VLQLFSLPLM PLSTSLGQSF SVAFKNFSPQ HSAGLARIVC SYLREHLKKT
810 820 830 840 850
KILLVSIEGT QLLKLESAIQ TKILRSLSCL EGMLSLSNFL LKGSASVISE
860 870 880 890 900
LSAADADVLK ELGITYKQTI WQMALCNDTK EDEKKSVDRG SDNSVSASSS
910 920 930 940 950
TAERESDEDS SNALAVRYTN PVSIRSSSSQ SIWGGDREFL SIVRSGEGIH
960 970 980 990 1000
GRTRHAIARM RGGRTRRHLE SFNFDSEIPA DLPVTSSSHE LKKKSTEVLI
1010 1020 1030 1040 1050
AEILNKLNCT LRFFFTALVK GFTSANRRRI DGASLSSASK TLGTALAKVF
1060 1070 1080 1090 1100
LEALNFDGYG AAAGHEKSLS VKCRYLGKVV DDITFLSFDT RRRVCFTAMV
1110 1120 1130 1140 1150
NSFYVHGTFK ELLTTFEATS QLLWTVPFSI PASSTENEKP GERNIWSHSK
1160 1170 1180 1190 1200
WLVDTLQNYC RALDYFVNST YLLSPTSQTQ LLVQPASVGL SIGLFPVPRE
1210 1220 1230 1240 1250
PETFVRNLQS QVLDVILPIW NHPMFPDCNP NFVASVTSLV THIYSGVVDA
1260 1270 1280 1290 1300
RENRSGVTRG INQRALPLQL DESIVGMIVE MGFSRSRAEI ALRRVGTNSV
1310 1320 1330 1340 1350
EMAMDWLFTN PEQPVQEDDE LAQALALSLG NSSETPKLED TEKPVDVPQE
1360 1370 1380 1390 1400
EAEPKEPPVD EVIAASVKLF QSDDSMAFPL MDLFVTLCNR NKGEDRPKIV
1410 1420 1430 1440 1450
SYLIQQLKLV QLDFSKDTGA LTMIPHILAL VLSEDDNTRE IAAQDGIVTV
1460 1470 1480 1490 1500
AIGILTDFNL KSESETEILA PKCISALLLV LSMMLQAQTK LSSEYVEGNQ
1510 1520 1530 1540 1550
GGSLVPSDSP QDSTAALKDA LSSDVAKGES NQALELIFGK STGYLTMEEG
1560 1570 1580 1590 1600
HKALLIACGL IKQHVPAMIM QAVLQLCARL TKSHALAIQF LENGGLSSLF
1610 1620 1630 1640 1650
NLPKKCCFPG YDTVASVIVR HLVEDPQTLQ IAMETEIRQT LSGKRHIGRV
1660 1670 1680 1690 1700
LPRTFLTTMA PVISRDPVVF MKAVASTCQL ESSGGRDFVI LSKEKEKPKV
1710 1720 1730 1740 1750
SGSEHGFSLN EPLGISENKL HDVSGKCSKS HRRVPANFIQ VIDQLIDLVL
1760 1770 1780 1790 1800
SFPRVKRQED GETNLISMEV DEPTTKVKGK SKVGEPEKAS SSRVGEPEKA
1810 1820 1830 1840 1850
EIPEKSEELA RVTFILKLLS DIVLMYSHGT SVILRRDTEI SQLRGSNLPD
1860 1870 1880 1890 1900
DSPGNGGLIY HVIHRLLPIS LEKFVGPEEW KEKLSEKASW FLVVLCSRSN
1910 1920 1930 1940 1950
EGRKRIINEL SRVLSVFASL GRSSSKSVLL PDKRVLAFAN LVYSILTKNS
1960 1970 1980 1990 2000
SSSSSNFPGC GCSPDVAKSM MDGGTIQCLT SILHVIDLDH PDAPKLVTLI
2010 2020 2030 2040 2050
LKSLETLTRA ANAAEQLKSE VPNEKKNRDS DERHDSHGNS TETEADELNQ
2060 2070 2080 2090 2100
NNSSLQQVTD AAGNGQEQAQ VSSQSAGERG SSQTQAMPQD MRIEGDETIL
2110 2120 2130 2140 2150
PEPIQMDFMR EEIEGDQIEM SFHVENRADD DVDDDMGDEG EDDEGDDEDA
2160 2170 2180 2190 2200
DLVEDGAGVM SLAGTDVEDP EDTGLGDEYN DDMVDEDDDD FHENRVIEVR
2210 2220 2230 2240 2250
WREALDGLDH FQILGRSGGG NGFIDDITAE PFEGVNVDDL FALRRPLGFE
2260 2270 2280 2290 2300
RRRQTGRSSL DRSGSEVHGF QHPLFSRPSQ TGNTASVSAS AGSISRHSEA
2310 2320 2330 2340 2350
GSYDVAQFYM FDTPVLPFDQ VPVDPFSARL AGGGAPPPLT DYSVVGMDSS
2360 2370 2380 2390 2400
RRGVGDSRWT DIGHPQPSSL SASIAQLIEE HFISNLRASA PVNTVVERET
2410 2420 2430 2440 2450
NTTEIQEQLH PDVPPSVGSE TVLGDGNEGG QQSEERELLN NNENVNNPPD
2460 2470 2480 2490 2500
VMAESFAQGQ ANLASPVSQD TGESLQQLEV MQPLPLNSTP NEIDRMEVGE
2510 2520 2530 2540 2550
GDGAPIDQVD HEAVHLISTA QGQPDTSSIQ NVSVTAIAPP VDDPDSNFQP
2560 2570 2580 2590 2600
SVDVDMSSDG AEGNQSVQPS PLDGDNNELS SMEATENVRN DEQVEEGSLD
2610 2620 2630 2640 2650
GRAPEVNAID PTFLEALPED LRAEVLASQQ AQSVQPPTYE PPPVDDIDPE
2660 2670 2680 2690 2700
FLAALPPDIQ TEVLAQQRAQ RMVQQSQGQA VDMDNASIIA TLPADLREEV
2710 2720 2730 2740 2750
LLTSSEAVLA ALPSPLLAEA QMLRDRAMSH YQARSSVFGS SHRLNNRRNG
2760 2770 2780 2790 2800
LGYNRLTGMD RGVGVTIGQR AVSSSADGLK VKEIEGDPLV NADALKSLIR
2810 2820 2830 2840 2850
LLRLAQPLGK GLLQRLLLNL CAHSFTRANL VQLLLDMIRP EMETSPSELA
2860 2870 2880 2890 2900
ITNPQRLYGC QSNVVYGRSQ LLNGLPPLVF RRVLEVLTYL ATNHSAVADM
2910 2920 2930 2940 2950
LFYFDSSLLS QLSSRKGKEK VTHVTDSRDL EIPLVVFLKL LNRPQLLQST
2960 2970 2980 2990 3000
SHLGLVMGLL QVVVYTAASR IEGWSPSSGV PEKLENKPVG EEASSETRKD
3010 3020 3030 3040 3050
AESELVGEAD LSVARRKNCA EIYNIFLQLP QSDLCNLCIL LGYEGLSDKI
3060 3070 3080 3090 3100
YSLAGEVLKK LAAVDVAHRK FFTKELSELA SSLSSSTVRE LATLSSKQKM
3110 3120 3130 3140 3150
SRSTGSMAGA SILRVLQVLS SLTSPIDESN VGTERETEQE EQNIMQRLNV
3160 3170 3180 3190 3200
ALEPLWHELS QCISMTELQL DHTAAASNIN PGDHVLGISP TSSLSPGTQR
3210 3220 3230 3240 3250
LLPLIEAFFV LCEKIQTPSM LQQDTNVTAG EVKESSAHGS SSKTSVDSQK
3260 3270 3280 3290 3300
KTDGSVTFSK FAEKHRRLLN SFIRQNPSLL EKSLSMMLKA PRLIDFDNKK
3310 3320 3330 3340 3350
AYFRSRIRHQ HDQHISGPLR ISVRRAYVLE DSYNQLRMRS PQDLKGRLNV
3360 3370 3380 3390 3400
QFQGEEGIDA GGLTREWYQL LSRVIFDKGA LLFTTVGNDA TFQPNPNSVY
3410 3420 3430 3440 3450
QTEHLSYFKF VGRMVAKALF DGQLLDVYFT RSFYKHILGV KVTYHDIEAV
3460 3470 3480 3490 3500
DPDYYKNLKW LLENDVSDIL DLTFSMDADE EKHILYEKTE VTDYELKPGG
3510 3520 3530 3540 3550
RNIRVTEETK HEYVDLVAGH ILTNAIRPQI NAFLEGFNEL IPRELVSIFN
3560 3570 3580 3590 3600
DKELELLISG LPEIDFDDLK ANTEYTSYTA GSPVIHWFWE VVKAFSKEDM
3610 3620 3630 3640 3650
ARFLQFVTGT SKVPLEGFKA LQGISGPQRL QIHKAYGAPE RLPSAHTCFN
3660 3670 3680
QLDLPEYQSK EQLQERLLLA IHEASEGFGF A
Length:3,681
Mass (Da):405,000
Last modified:December 4, 2007 - v3
Checksum:i3954A6ABDD574189
GO

Sequence cautioni

The sequence AAF79338 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAC42550 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti865T → S in AAF36454 (PubMed:10571878).Curated1
Sequence conflicti1186A → T in AAF36454 (PubMed:10571878).Curated1
Sequence conflicti1207N → K in AAF36454 (PubMed:10571878).Curated1
Sequence conflicti3027L → S in AAF36454 (PubMed:10571878).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF127564 Genomic DNA Translation: AAF36454.1
AC002304 Genomic DNA Translation: AAF79338.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE33309.2
AK117912 mRNA Translation: BAC42550.1 Different initiation.
PIRiH96599
RefSeqiNP_001185245.1, NM_001198316.2
UniGeneiAt.48310
At.66950

Genome annotation databases

EnsemblPlantsiAT1G55860.1; AT1G55860.1; AT1G55860
GeneIDi842036
GrameneiAT1G55860.1; AT1G55860.1; AT1G55860
KEGGiath:AT1G55860

Similar proteinsi

Entry informationi

Entry nameiUPL1_ARATH
AccessioniPrimary (citable) accession number: Q8GY23
Secondary accession number(s): F4I1Y3, Q9LG27, Q9M7K7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: December 4, 2007
Last modified: April 25, 2018
This is version 123 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome