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UniProtKB - Q8GY23 (UPL1_ARATH)

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Protein

E3 ubiquitin-protein ligase UPL1

Gene

UPL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins.1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3648Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ubiquitin protein ligase activity Source: GO_Central
  • ubiquitin-protein transferase activity Source: TAIR
View the complete GO annotation on QuickGO ...

GO - Biological processi

  • protein ubiquitination Source: TAIR
View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-ATH-6798695 Neutrophil degranulation
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase UPL1 (EC:2.3.2.26)
Short name:
Ubiquitin-protein ligase 1
Alternative name(s):
HECT-type E3 ubiquitin transferase UPL1
Gene namesi
Name:UPL1
Ordered Locus Names:At1g55860
ORF Names:F14J16.14, F14J16.37
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G55860
TAIRilocus:2012060 AT1G55860

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi3648C → S or A: Abolishes ability to conjugate ubiquitin in vitro. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001203431 – 3681E3 ubiquitin-protein ligase UPL1Add BLAST3681

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2598PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8GY23
PRIDEiQ8GY23

PTM databases

iPTMnetiQ8GY23

Expressioni

Tissue specificityi

Widely expressed. Expressed in root, stem, cauline and rosette leaf, seedling and flower (at protein level).1 Publication

Developmental stagei

Constitutively expressed throughout development post-germination (at protein level).1 Publication

Gene expression databases

ExpressionAtlasiQ8GY23 baseline and differential
GenevisibleiQ8GY23 AT

Interactioni

Protein-protein interaction databases

BioGridi272611 interactor.
STRINGi3702.AT1G55860.1

Structurei

3D structure databases

ProteinModelPortaliQ8GY23
SMRiQ8GY23
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1269 – 1310UBAPROSITE-ProRule annotationAdd BLAST42
Domaini1316 – 1335UIMPROSITE-ProRule annotationAdd BLAST20
Domaini3340 – 3681HECTPROSITE-ProRule annotationAdd BLAST342

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi2129 – 2190Asp-richAdd BLAST62

Sequence similaritiesi

Belongs to the UPL family. TOM1/PTR1 subfamily.Curated

Phylogenomic databases

eggNOGiKOG0939 Eukaryota
COG5021 LUCA
HOGENOMiHOG000084686
InParanoidiQ8GY23
KOiK10592
OrthoDBiEOG0936000C
PhylomeDBiQ8GY23

Family and domain databases

CDDicd00078 HECTc, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR025527 DUF4414
IPR010309 E3_Ub_ligase_DUF908
IPR010314 E3_Ub_ligase_DUF913
IPR000569 HECT_dom
IPR035983 Hect_E3_ubiquitin_ligase
IPR015940 UBA
IPR009060 UBA-like_sf
IPR003903 UIM_dom
PfamiView protein in Pfam
PF14377 DUF4414, 1 hit
PF06012 DUF908, 2 hits
PF06025 DUF913, 1 hit
PF00632 HECT, 1 hit
PF00627 UBA, 1 hit
SMARTiView protein in SMART
SM00119 HECTc, 1 hit
SM00165 UBA, 1 hit
SUPFAMiSSF46934 SSF46934, 1 hit
SSF48371 SSF48371, 11 hits
SSF56204 SSF56204, 1 hit
PROSITEiView protein in PROSITE
PS50237 HECT, 1 hit
PS50030 UBA, 1 hit
PS50330 UIM, 1 hit

Sequencei

Sequence statusi: Complete.

Q8GY23-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLRRRRASE VPSKIKSFIN SVTSVPLELI HEPLACFRWE FDKGDFHHWV 
        60         70         80         90        100
DLFNYFDTFF EKHVQVRKDL HIEENFEESD PPFPKDAVLQ VLRVIRVVLE 
       110        120        130        140        150
NCTNKHFYSS YEQHLSLLLA STDADVVEAC LQTLAAFLKR QIGKYSIRDA 
       160        170        180        190        200
SLNSKLFSLA QGWGGKEEGL GLTSCAAENS CDQVSLQLGR TLHFEFYPSD 
       210        220        230        240        250
ESPSELPGGL QVIHVPDVSI CAESDLELLN KLVIDHNVPP SLRFALLTRM 
       260        270        280        290        300
RFARAFSSLA TRQQFTCIRL YAFVVLVQAS GDTENVVSFF NGEPEFVNEL 
       310        320        330        340        350
VTLVSYEDTV PEKIRILCLL SLVALSQDRT RQPTVLTAVT SGGHRGLLSG 
       360        370        380        390        400
LMQKAIDSVV CITSKWSLAF AEALLSLVTV LVSSSSGCSA MREAGLIPTL 
       410        420        430        440        450
VPLIKDTDPQ HLHLVSAAVH ILEAFMDYSN PAAALFRDLG GLDDTIFRLK 
       460        470        480        490        500
LEVSRTEDDV KEKNCSSDSN GPDTEQLPYS EALISYHRRL LLKALLRAIS 
       510        520        530        540        550
LGTYAPGNTN LYGSEESLLP ECLCIIFRRA KDFGGGVFSL AATVMSDLIH 
       560        570        580        590        600
KDPTCFNALD SAGLTSTFLD AISDEVICSA EAITCIPQCL DALCLNNSGL 
       610        620        630        640        650
QAVKDRNALR CFVKIFTSPS YLRALTGDTP GSLSSGLDEL LRHQSSLRTY 
       660        670        680        690        700
GVDMFIEILN SMLIIGSGME ATTSKSADVP TSAAPVPMEI DVDEKSLAVS 
       710        720        730        740        750
DEAEPSSDTS PANIELFLPD CVCNVARLFE TVLQNAEVCS LFVEKKGIDA 
       760        770        780        790        800
VLQLFSLPLM PLSTSLGQSF SVAFKNFSPQ HSAGLARIVC SYLREHLKKT 
       810        820        830        840        850
KILLVSIEGT QLLKLESAIQ TKILRSLSCL EGMLSLSNFL LKGSASVISE 
       860        870        880        890        900
LSAADADVLK ELGITYKQTI WQMALCNDTK EDEKKSVDRG SDNSVSASSS 
       910        920        930        940        950
TAERESDEDS SNALAVRYTN PVSIRSSSSQ SIWGGDREFL SIVRSGEGIH 
       960        970        980        990       1000
GRTRHAIARM RGGRTRRHLE SFNFDSEIPA DLPVTSSSHE LKKKSTEVLI 
      1010       1020       1030       1040       1050
AEILNKLNCT LRFFFTALVK GFTSANRRRI DGASLSSASK TLGTALAKVF 
      1060       1070       1080       1090       1100
LEALNFDGYG AAAGHEKSLS VKCRYLGKVV DDITFLSFDT RRRVCFTAMV 
      1110       1120       1130       1140       1150
NSFYVHGTFK ELLTTFEATS QLLWTVPFSI PASSTENEKP GERNIWSHSK 
      1160       1170       1180       1190       1200
WLVDTLQNYC RALDYFVNST YLLSPTSQTQ LLVQPASVGL SIGLFPVPRE 
      1210       1220       1230       1240       1250
PETFVRNLQS QVLDVILPIW NHPMFPDCNP NFVASVTSLV THIYSGVVDA 
      1260       1270       1280       1290       1300
RENRSGVTRG INQRALPLQL DESIVGMIVE MGFSRSRAEI ALRRVGTNSV 
      1310       1320       1330       1340       1350
EMAMDWLFTN PEQPVQEDDE LAQALALSLG NSSETPKLED TEKPVDVPQE 
      1360       1370       1380       1390       1400
EAEPKEPPVD EVIAASVKLF QSDDSMAFPL MDLFVTLCNR NKGEDRPKIV 
      1410       1420       1430       1440       1450
SYLIQQLKLV QLDFSKDTGA LTMIPHILAL VLSEDDNTRE IAAQDGIVTV 
      1460       1470       1480       1490       1500
AIGILTDFNL KSESETEILA PKCISALLLV LSMMLQAQTK LSSEYVEGNQ 
      1510       1520       1530       1540       1550
GGSLVPSDSP QDSTAALKDA LSSDVAKGES NQALELIFGK STGYLTMEEG 
      1560       1570       1580       1590       1600
HKALLIACGL IKQHVPAMIM QAVLQLCARL TKSHALAIQF LENGGLSSLF 
      1610       1620       1630       1640       1650
NLPKKCCFPG YDTVASVIVR HLVEDPQTLQ IAMETEIRQT LSGKRHIGRV 
      1660       1670       1680       1690       1700
LPRTFLTTMA PVISRDPVVF MKAVASTCQL ESSGGRDFVI LSKEKEKPKV 
      1710       1720       1730       1740       1750
SGSEHGFSLN EPLGISENKL HDVSGKCSKS HRRVPANFIQ VIDQLIDLVL 
      1760       1770       1780       1790       1800
SFPRVKRQED GETNLISMEV DEPTTKVKGK SKVGEPEKAS SSRVGEPEKA 
      1810       1820       1830       1840       1850
EIPEKSEELA RVTFILKLLS DIVLMYSHGT SVILRRDTEI SQLRGSNLPD 
      1860       1870       1880       1890       1900
DSPGNGGLIY HVIHRLLPIS LEKFVGPEEW KEKLSEKASW FLVVLCSRSN 
      1910       1920       1930       1940       1950
EGRKRIINEL SRVLSVFASL GRSSSKSVLL PDKRVLAFAN LVYSILTKNS 
      1960       1970       1980       1990       2000
SSSSSNFPGC GCSPDVAKSM MDGGTIQCLT SILHVIDLDH PDAPKLVTLI 
      2010       2020       2030       2040       2050
LKSLETLTRA ANAAEQLKSE VPNEKKNRDS DERHDSHGNS TETEADELNQ 
      2060       2070       2080       2090       2100
NNSSLQQVTD AAGNGQEQAQ VSSQSAGERG SSQTQAMPQD MRIEGDETIL 
      2110       2120       2130       2140       2150
PEPIQMDFMR EEIEGDQIEM SFHVENRADD DVDDDMGDEG EDDEGDDEDA 
      2160       2170       2180       2190       2200
DLVEDGAGVM SLAGTDVEDP EDTGLGDEYN DDMVDEDDDD FHENRVIEVR 
      2210       2220       2230       2240       2250
WREALDGLDH FQILGRSGGG NGFIDDITAE PFEGVNVDDL FALRRPLGFE 
      2260       2270       2280       2290       2300
RRRQTGRSSL DRSGSEVHGF QHPLFSRPSQ TGNTASVSAS AGSISRHSEA 
      2310       2320       2330       2340       2350
GSYDVAQFYM FDTPVLPFDQ VPVDPFSARL AGGGAPPPLT DYSVVGMDSS 
      2360       2370       2380       2390       2400
RRGVGDSRWT DIGHPQPSSL SASIAQLIEE HFISNLRASA PVNTVVERET 
      2410       2420       2430       2440       2450
NTTEIQEQLH PDVPPSVGSE TVLGDGNEGG QQSEERELLN NNENVNNPPD 
      2460       2470       2480       2490       2500
VMAESFAQGQ ANLASPVSQD TGESLQQLEV MQPLPLNSTP NEIDRMEVGE 
      2510       2520       2530       2540       2550
GDGAPIDQVD HEAVHLISTA QGQPDTSSIQ NVSVTAIAPP VDDPDSNFQP 
      2560       2570       2580       2590       2600
SVDVDMSSDG AEGNQSVQPS PLDGDNNELS SMEATENVRN DEQVEEGSLD 
      2610       2620       2630       2640       2650
GRAPEVNAID PTFLEALPED LRAEVLASQQ AQSVQPPTYE PPPVDDIDPE 
      2660       2670       2680       2690       2700
FLAALPPDIQ TEVLAQQRAQ RMVQQSQGQA VDMDNASIIA TLPADLREEV 
      2710       2720       2730       2740       2750
LLTSSEAVLA ALPSPLLAEA QMLRDRAMSH YQARSSVFGS SHRLNNRRNG 
      2760       2770       2780       2790       2800
LGYNRLTGMD RGVGVTIGQR AVSSSADGLK VKEIEGDPLV NADALKSLIR 
      2810       2820       2830       2840       2850
LLRLAQPLGK GLLQRLLLNL CAHSFTRANL VQLLLDMIRP EMETSPSELA 
      2860       2870       2880       2890       2900
ITNPQRLYGC QSNVVYGRSQ LLNGLPPLVF RRVLEVLTYL ATNHSAVADM 
      2910       2920       2930       2940       2950
LFYFDSSLLS QLSSRKGKEK VTHVTDSRDL EIPLVVFLKL LNRPQLLQST 
      2960       2970       2980       2990       3000
SHLGLVMGLL QVVVYTAASR IEGWSPSSGV PEKLENKPVG EEASSETRKD 
      3010       3020       3030       3040       3050
AESELVGEAD LSVARRKNCA EIYNIFLQLP QSDLCNLCIL LGYEGLSDKI 
      3060       3070       3080       3090       3100
YSLAGEVLKK LAAVDVAHRK FFTKELSELA SSLSSSTVRE LATLSSKQKM 
      3110       3120       3130       3140       3150
SRSTGSMAGA SILRVLQVLS SLTSPIDESN VGTERETEQE EQNIMQRLNV 
      3160       3170       3180       3190       3200
ALEPLWHELS QCISMTELQL DHTAAASNIN PGDHVLGISP TSSLSPGTQR 
      3210       3220       3230       3240       3250
LLPLIEAFFV LCEKIQTPSM LQQDTNVTAG EVKESSAHGS SSKTSVDSQK 
      3260       3270       3280       3290       3300
KTDGSVTFSK FAEKHRRLLN SFIRQNPSLL EKSLSMMLKA PRLIDFDNKK 
      3310       3320       3330       3340       3350
AYFRSRIRHQ HDQHISGPLR ISVRRAYVLE DSYNQLRMRS PQDLKGRLNV 
      3360       3370       3380       3390       3400
QFQGEEGIDA GGLTREWYQL LSRVIFDKGA LLFTTVGNDA TFQPNPNSVY 
      3410       3420       3430       3440       3450
QTEHLSYFKF VGRMVAKALF DGQLLDVYFT RSFYKHILGV KVTYHDIEAV 
      3460       3470       3480       3490       3500
DPDYYKNLKW LLENDVSDIL DLTFSMDADE EKHILYEKTE VTDYELKPGG 
      3510       3520       3530       3540       3550
RNIRVTEETK HEYVDLVAGH ILTNAIRPQI NAFLEGFNEL IPRELVSIFN 
      3560       3570       3580       3590       3600
DKELELLISG LPEIDFDDLK ANTEYTSYTA GSPVIHWFWE VVKAFSKEDM 
      3610       3620       3630       3640       3650
ARFLQFVTGT SKVPLEGFKA LQGISGPQRL QIHKAYGAPE RLPSAHTCFN 
      3660       3670       3680 
QLDLPEYQSK EQLQERLLLA IHEASEGFGF A
Length:3,681
Mass (Da):405,000
Last modified:December 4, 2007 - v3
Checksum:i3954A6ABDD574189
GO

Sequence cautioni

The sequence AAF79338 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAC42550 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti865T → S in AAF36454 (PubMed:10571878).Curated1
Sequence conflicti1186A → T in AAF36454 (PubMed:10571878).Curated1
Sequence conflicti1207N → K in AAF36454 (PubMed:10571878).Curated1
Sequence conflicti3027L → S in AAF36454 (PubMed:10571878).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF127564 Genomic DNA Translation: AAF36454.1
AC002304 Genomic DNA Translation: AAF79338.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE33309.2
AK117912 mRNA Translation: BAC42550.1 Different initiation.
PIRiH96599
RefSeqiNP_001185245.1, NM_001198316.2
UniGeneiAt.48310
At.66950

Genome annotation databases

EnsemblPlantsiAT1G55860.1; AT1G55860.1; AT1G55860
GeneIDi842036
GrameneiAT1G55860.1; AT1G55860.1; AT1G55860
KEGGiath:AT1G55860

Similar proteinsi

Entry informationi

Entry nameiUPL1_ARATH
AccessioniPrimary (citable) accession number: Q8GY23
Secondary accession number(s): F4I1Y3, Q9LG27, Q9M7K7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: December 4, 2007
Last modified: April 25, 2018
This is version 123 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome