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Q8GXX0

- ERV1_ARATH

UniProt

Q8GXX0 - ERV1_ARATH

Protein

FAD-linked sulfhydryl oxidase ERV1

Gene

ERV1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. Oxidizes thioredoxin in vitro.1 PublicationPROSITE-ProRule annotation

    Catalytic activityi

    2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

    Cofactori

    FAD.1 PublicationPROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei76 – 761FAD1 Publication
    Binding sitei160 – 1601FAD1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi81 – 844FAD1 Publication
    Nucleotide bindingi121 – 1255FAD1 Publication
    Nucleotide bindingi148 – 1558FAD1 Publication
    Nucleotide bindingi171 – 1722FAD1 Publication

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: UniProtKB
    2. identical protein binding Source: UniProtKB
    3. thiol oxidase activity Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciARA:AT1G49880-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    FAD-linked sulfhydryl oxidase ERV1 (EC:1.8.3.2)
    Short name:
    AtErv1
    Alternative name(s):
    Mitochondrial sulfhydryl oxidase ERV1
    Gene namesi
    Name:ERV1
    Ordered Locus Names:At1g49880
    ORF Names:F10F5.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G49880.

    Subcellular locationi

    Mitochondrion 1 Publication

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi177 – 1771C → A: Loss the capacity to oxidize thioredoxin; when associated with A-182. 1 Publication
    Mutagenesisi182 – 1821C → A: Loss the capacity to oxidize thioredoxin; when associated with A-177. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 191191FAD-linked sulfhydryl oxidase ERV1PRO_0000401377Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi119 ↔ 122Redox-activePROSITE-ProRule annotation
    Disulfide bondi148 ↔ 1651 PublicationPROSITE-ProRule annotation
    Disulfide bondi177 ↔ 1821 PublicationPROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiQ8GXX0.
    PRIDEiQ8GXX0.

    Expressioni

    Gene expression databases

    GenevestigatoriQ8GXX0.

    Interactioni

    Subunit structurei

    Homodimer.2 Publications

    Protein-protein interaction databases

    STRINGi3702.AT1G49880.1-P.

    Structurei

    Secondary structure

    1
    191
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi76 – 9318
    Helixi100 – 11617
    Helixi120 – 13213
    Helixi140 – 15718
    Helixi168 – 1714

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2HJ3X-ray2.50A/B71-191[»]
    ProteinModelPortaliQ8GXX0.
    SMRiQ8GXX0. Positions 73-173.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8GXX0.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini72 – 172101ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi177 – 1826Required for dimerization and substrate specificity

    Sequence similaritiesi

    Contains 1 ERV/ALR sulfhydryl oxidase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5054.
    HOGENOMiHOG000120834.
    InParanoidiQ8GXX0.
    KOiK17783.
    OMAiIFPCQRV.
    PhylomeDBiQ8GXX0.

    Family and domain databases

    Gene3Di1.20.120.310. 1 hit.
    InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
    [Graphical view]
    PfamiPF04777. Evr1_Alr. 1 hit.
    [Graphical view]
    SUPFAMiSSF69000. SSF69000. 1 hit.
    PROSITEiPS51324. ERV_ALR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8GXX0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGEKPWQPLL QSFEKLSNCV QTHLSNFIGI KNTPPSSQST IQNPIISLDS    50
    SPPIATNSSS LQKLPLKDKS TGPVTKEDLG RATWTFLHTL AAQYPEKPTR 100
    QQKKDVKELM TILSRMYPCR ECADHFKEIL RSNPAQAGSQ EEFSQWLCHV 150
    HNTVNRSLGK LVFPCERVDA RWGKLECEQK SCDLHGTSMD F 191
    Length:191
    Mass (Da):21,600
    Last modified:March 1, 2003 - v1
    Checksum:i7CC7FEA44C0D4B38
    GO

    Sequence cautioni

    The sequence AAG51780.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti35 – 362PS → R in AAM63908. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ551263 mRNA. Translation: CAD83013.1.
    AC079674 Genomic DNA. Translation: AAG51780.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE32487.1.
    AK117991 mRNA. Translation: BAC42626.1.
    BT003718 mRNA. Translation: AAO39946.1.
    AY086861 mRNA. Translation: AAM63908.1.
    PIRiG96535.
    RefSeqiNP_564557.1. NM_103875.3.
    UniGeneiAt.38141.

    Genome annotation databases

    EnsemblPlantsiAT1G49880.1; AT1G49880.1; AT1G49880.
    GeneIDi841411.
    KEGGiath:AT1G49880.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ551263 mRNA. Translation: CAD83013.1 .
    AC079674 Genomic DNA. Translation: AAG51780.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE32487.1 .
    AK117991 mRNA. Translation: BAC42626.1 .
    BT003718 mRNA. Translation: AAO39946.1 .
    AY086861 mRNA. Translation: AAM63908.1 .
    PIRi G96535.
    RefSeqi NP_564557.1. NM_103875.3.
    UniGenei At.38141.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2HJ3 X-ray 2.50 A/B 71-191 [» ]
    ProteinModelPortali Q8GXX0.
    SMRi Q8GXX0. Positions 73-173.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT1G49880.1-P.

    Proteomic databases

    PaxDbi Q8GXX0.
    PRIDEi Q8GXX0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G49880.1 ; AT1G49880.1 ; AT1G49880 .
    GeneIDi 841411.
    KEGGi ath:AT1G49880.

    Organism-specific databases

    TAIRi AT1G49880.

    Phylogenomic databases

    eggNOGi COG5054.
    HOGENOMi HOG000120834.
    InParanoidi Q8GXX0.
    KOi K17783.
    OMAi IFPCQRV.
    PhylomeDBi Q8GXX0.

    Enzyme and pathway databases

    BioCyci ARA:AT1G49880-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q8GXX0.
    PROi Q8GXX0.

    Gene expression databases

    Genevestigatori Q8GXX0.

    Family and domain databases

    Gene3Di 1.20.120.310. 1 hit.
    InterProi IPR017905. ERV/ALR_sulphydryl_oxidase.
    [Graphical view ]
    Pfami PF04777. Evr1_Alr. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69000. SSF69000. 1 hit.
    PROSITEi PS51324. ERV_ALR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Unique features of plant mitochondrial sulfhydryl oxidase."
      Levitan A., Danon A., Lisowsky T.
      J. Biol. Chem. 279:20002-20008(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    7. "Gain of function in an ERV/ALR sulfhydryl oxidase by molecular engineering of the shuttle disulfide."
      Vitu E., Bentzur M., Lisowsky T., Kaiser C.A., Fass D.
      J. Mol. Biol. 362:89-101(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 71-191 IN COMPLEX WITH FAD, SUBUNIT, DISULFIDE BONDS, MUTAGENESIS OF CYS-177 AND CYS-182.

    Entry informationi

    Entry nameiERV1_ARATH
    AccessioniPrimary (citable) accession number: Q8GXX0
    Secondary accession number(s): Q8LC15, Q9C6C6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 30, 2010
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3