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Protein

FAD-linked sulfhydryl oxidase ERV1

Gene

ERV1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation (By similarity) (PubMed:14996837). Oxidizes thioredoxin in vitro (PubMed:14996837). Required for the import and folding of small cysteine-containing proteins in the mitochondrial intermembrane space. Forms a redox cycle with MIA40 that involves a disulfide relay system. Important for maintaining the cysteine residues in MIA40 in an oxidized state (By similarity).PROSITE-ProRule annotationBy similarity1 Publication

Catalytic activityi

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactori

FADPROSITE-ProRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei76FAD1 Publication1
Binding sitei160FAD1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi81 – 84FAD1 Publication4
Nucleotide bindingi121 – 125FAD1 Publication5
Nucleotide bindingi148 – 155FAD1 Publication8
Nucleotide bindingi171 – 172FAD1 Publication2

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: UniProtKB
  • flavin-linked sulfhydryl oxidase activity Source: GO_Central
  • identical protein binding Source: UniProtKB
  • protein disulfide oxidoreductase activity Source: GO_Central
  • thiol oxidase activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciARA:AT1G49880-MONOMER.
BRENDAi1.8.3.2. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
FAD-linked sulfhydryl oxidase ERV1 (EC:1.8.3.2)
Short name:
AtErv1
Alternative name(s):
Mitochondrial sulfhydryl oxidase ERV1
Gene namesi
Name:ERV1
Ordered Locus Names:At1g49880
ORF Names:F10F5.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G49880.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality when homozygous.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi177C → A: Loss the capacity to oxidize thioredoxin; when associated with A-182. 1 Publication1
Mutagenesisi182C → A: Loss the capacity to oxidize thioredoxin; when associated with A-177. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004013771 – 191FAD-linked sulfhydryl oxidase ERV1Add BLAST191

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi119 ↔ 122Redox-activePROSITE-ProRule annotation1 Publication
Disulfide bondi148 ↔ 165PROSITE-ProRule annotation2 Publications
Disulfide bondi177 ↔ 1822 Publications

Post-translational modificationi

Contains three disulfide bonds; one catalytic disulfide (Cys-119 to Cys-122), one structural disulfide (Cys-148 to Cys-165), and one shuttle disulfide (Cys-177 to Cys-182).1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ8GXX0.

Expressioni

Gene expression databases

GenevisibleiQ8GXX0. AT.

Interactioni

Subunit structurei

Homodimer.2 Publications

GO - Molecular functioni

  • identical protein binding Source: UniProtKB

Protein-protein interaction databases

STRINGi3702.AT1G49880.1.

Structurei

Secondary structure

1191
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi76 – 93Combined sources18
Helixi100 – 116Combined sources17
Helixi120 – 132Combined sources13
Helixi140 – 157Combined sources18
Helixi168 – 171Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HJ3X-ray2.50A/B71-191[»]
ProteinModelPortaliQ8GXX0.
SMRiQ8GXX0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8GXX0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 172ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd BLAST101

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi177 – 182Required for dimerization and substrate specificity6

Sequence similaritiesi

Contains 1 ERV/ALR sulfhydryl oxidase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3355. Eukaryota.
COG5054. LUCA.
HOGENOMiHOG000120834.
InParanoidiQ8GXX0.
KOiK17783.
OMAiEGSIDIM.
OrthoDBiEOG09360SPX.
PhylomeDBiQ8GXX0.

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
[Graphical view]
PfamiPF04777. Evr1_Alr. 1 hit.
[Graphical view]
SUPFAMiSSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8GXX0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEKPWQPLL QSFEKLSNCV QTHLSNFIGI KNTPPSSQST IQNPIISLDS
60 70 80 90 100
SPPIATNSSS LQKLPLKDKS TGPVTKEDLG RATWTFLHTL AAQYPEKPTR
110 120 130 140 150
QQKKDVKELM TILSRMYPCR ECADHFKEIL RSNPAQAGSQ EEFSQWLCHV
160 170 180 190
HNTVNRSLGK LVFPCERVDA RWGKLECEQK SCDLHGTSMD F
Length:191
Mass (Da):21,600
Last modified:March 1, 2003 - v1
Checksum:i7CC7FEA44C0D4B38
GO

Sequence cautioni

The sequence AAG51780 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35 – 36PS → R in AAM63908 (Ref. 6) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ551263 mRNA. Translation: CAD83013.1.
AC079674 Genomic DNA. Translation: AAG51780.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32487.1.
AK117991 mRNA. Translation: BAC42626.1.
BT003718 mRNA. Translation: AAO39946.1.
AY086861 mRNA. Translation: AAM63908.1.
PIRiG96535.
RefSeqiNP_564557.1. NM_103875.4.
UniGeneiAt.38141.

Genome annotation databases

EnsemblPlantsiAT1G49880.1; AT1G49880.1; AT1G49880.
GeneIDi841411.
GrameneiAT1G49880.1; AT1G49880.1; AT1G49880.
KEGGiath:AT1G49880.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ551263 mRNA. Translation: CAD83013.1.
AC079674 Genomic DNA. Translation: AAG51780.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32487.1.
AK117991 mRNA. Translation: BAC42626.1.
BT003718 mRNA. Translation: AAO39946.1.
AY086861 mRNA. Translation: AAM63908.1.
PIRiG96535.
RefSeqiNP_564557.1. NM_103875.4.
UniGeneiAt.38141.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HJ3X-ray2.50A/B71-191[»]
ProteinModelPortaliQ8GXX0.
SMRiQ8GXX0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G49880.1.

Proteomic databases

PaxDbiQ8GXX0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G49880.1; AT1G49880.1; AT1G49880.
GeneIDi841411.
GrameneiAT1G49880.1; AT1G49880.1; AT1G49880.
KEGGiath:AT1G49880.

Organism-specific databases

TAIRiAT1G49880.

Phylogenomic databases

eggNOGiKOG3355. Eukaryota.
COG5054. LUCA.
HOGENOMiHOG000120834.
InParanoidiQ8GXX0.
KOiK17783.
OMAiEGSIDIM.
OrthoDBiEOG09360SPX.
PhylomeDBiQ8GXX0.

Enzyme and pathway databases

BioCyciARA:AT1G49880-MONOMER.
BRENDAi1.8.3.2. 399.

Miscellaneous databases

EvolutionaryTraceiQ8GXX0.
PROiQ8GXX0.

Gene expression databases

GenevisibleiQ8GXX0. AT.

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
[Graphical view]
PfamiPF04777. Evr1_Alr. 1 hit.
[Graphical view]
SUPFAMiSSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiERV1_ARATH
AccessioniPrimary (citable) accession number: Q8GXX0
Secondary accession number(s): Q8LC15, Q9C6C6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.