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Protein

FAD-linked sulfhydryl oxidase ERV1

Gene

ERV1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation (By similarity) (PubMed:14996837). Oxidizes thioredoxin in vitro (PubMed:14996837). Required for the import and folding of small cysteine-containing proteins in the mitochondrial intermembrane space. Forms a redox cycle with MIA40 that involves a disulfide relay system. Important for maintaining the cysteine residues in MIA40 in an oxidized state (By similarity).PROSITE-ProRule annotationBy similarity1 Publication

Catalytic activityi

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactori

FADPROSITE-ProRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei76FAD1 Publication1
Binding sitei160FAD1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi81 – 84FAD1 Publication4
Nucleotide bindingi121 – 125FAD1 Publication5
Nucleotide bindingi148 – 155FAD1 Publication8
Nucleotide bindingi171 – 172FAD1 Publication2

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: UniProtKB
  • flavin-linked sulfhydryl oxidase activity Source: GO_Central
  • identical protein binding Source: UniProtKB
  • protein disulfide oxidoreductase activity Source: GO_Central
  • thiol oxidase activity Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein

Enzyme and pathway databases

BioCyciARA:AT1G49880-MONOMER
BRENDAi1.8.3.2 399

Names & Taxonomyi

Protein namesi
Recommended name:
FAD-linked sulfhydryl oxidase ERV1 (EC:1.8.3.2)
Short name:
AtErv1
Alternative name(s):
Mitochondrial sulfhydryl oxidase ERV1
Gene namesi
Name:ERV1
Ordered Locus Names:At1g49880
ORF Names:F10F5.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G49880
TAIRilocus:2007283 AT1G49880

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality when homozygous.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi177C → A: Loss the capacity to oxidize thioredoxin; when associated with A-182. 1 Publication1
Mutagenesisi182C → A: Loss the capacity to oxidize thioredoxin; when associated with A-177. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004013771 – 191FAD-linked sulfhydryl oxidase ERV1Add BLAST191

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi119 ↔ 122Redox-activePROSITE-ProRule annotation1 Publication
Disulfide bondi148 ↔ 165PROSITE-ProRule annotation2 Publications
Disulfide bondi177 ↔ 1822 Publications

Post-translational modificationi

Contains three disulfide bonds; one catalytic disulfide (Cys-119 to Cys-122), one structural disulfide (Cys-148 to Cys-165), and one shuttle disulfide (Cys-177 to Cys-182).1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ8GXX0

Expressioni

Gene expression databases

ExpressionAtlasiQ8GXX0 baseline and differential
GenevisibleiQ8GXX0 AT

Interactioni

Subunit structurei

Homodimer.2 Publications

GO - Molecular functioni

  • identical protein binding Source: UniProtKB

Protein-protein interaction databases

STRINGi3702.AT1G49880.1

Structurei

Secondary structure

1191
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi76 – 93Combined sources18
Helixi100 – 116Combined sources17
Helixi120 – 132Combined sources13
Helixi140 – 157Combined sources18
Helixi168 – 171Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HJ3X-ray2.50A/B71-191[»]
ProteinModelPortaliQ8GXX0
SMRiQ8GXX0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8GXX0

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 172ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd BLAST101

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi177 – 182Required for dimerization and substrate specificity6

Phylogenomic databases

eggNOGiKOG3355 Eukaryota
COG5054 LUCA
HOGENOMiHOG000120834
InParanoidiQ8GXX0
KOiK17783
OMAiSHAQFSH
OrthoDBiEOG09360SPX
PhylomeDBiQ8GXX0

Family and domain databases

Gene3Di1.20.120.310, 1 hit
InterProiView protein in InterPro
IPR036774 ERV/ALR_sulphydryl_oxid_sf
IPR017905 ERV/ALR_sulphydryl_oxidase
PfamiView protein in Pfam
PF04777 Evr1_Alr, 1 hit
SUPFAMiSSF69000 SSF69000, 1 hit
PROSITEiView protein in PROSITE
PS51324 ERV_ALR, 1 hit

Sequencei

Sequence statusi: Complete.

Q8GXX0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEKPWQPLL QSFEKLSNCV QTHLSNFIGI KNTPPSSQST IQNPIISLDS
60 70 80 90 100
SPPIATNSSS LQKLPLKDKS TGPVTKEDLG RATWTFLHTL AAQYPEKPTR
110 120 130 140 150
QQKKDVKELM TILSRMYPCR ECADHFKEIL RSNPAQAGSQ EEFSQWLCHV
160 170 180 190
HNTVNRSLGK LVFPCERVDA RWGKLECEQK SCDLHGTSMD F
Length:191
Mass (Da):21,600
Last modified:March 1, 2003 - v1
Checksum:i7CC7FEA44C0D4B38
GO

Sequence cautioni

The sequence AAG51780 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35 – 36PS → R in AAM63908 (Ref. 6) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ551263 mRNA Translation: CAD83013.1
AC079674 Genomic DNA Translation: AAG51780.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE32487.1
AK117991 mRNA Translation: BAC42626.1
BT003718 mRNA Translation: AAO39946.1
AY086861 mRNA Translation: AAM63908.1
PIRiG96535
RefSeqiNP_564557.1, NM_103875.4
UniGeneiAt.38141

Genome annotation databases

EnsemblPlantsiAT1G49880.1; AT1G49880.1; AT1G49880
GeneIDi841411
GrameneiAT1G49880.1; AT1G49880.1; AT1G49880
KEGGiath:AT1G49880

Similar proteinsi

Entry informationi

Entry nameiERV1_ARATH
AccessioniPrimary (citable) accession number: Q8GXX0
Secondary accession number(s): Q8LC15, Q9C6C6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: March 1, 2003
Last modified: April 25, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health