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Protein

FAD-linked sulfhydryl oxidase ERV1

Gene

ERV1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. Oxidizes thioredoxin in vitro.PROSITE-ProRule annotation1 Publication

Catalytic activityi

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactori

FADPROSITE-ProRule annotation1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei76 – 761FAD1 Publication
Binding sitei160 – 1601FAD1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi81 – 844FAD1 Publication
Nucleotide bindingi121 – 1255FAD1 Publication
Nucleotide bindingi148 – 1558FAD1 Publication
Nucleotide bindingi171 – 1722FAD1 Publication

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: UniProtKB
  • identical protein binding Source: UniProtKB
  • thiol oxidase activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciARA:AT1G49880-MONOMER.
BRENDAi1.8.3.2. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
FAD-linked sulfhydryl oxidase ERV1 (EC:1.8.3.2)
Short name:
AtErv1
Alternative name(s):
Mitochondrial sulfhydryl oxidase ERV1
Gene namesi
Name:ERV1
Ordered Locus Names:At1g49880
ORF Names:F10F5.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G49880.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi177 – 1771C → A: Loss the capacity to oxidize thioredoxin; when associated with A-182. 1 Publication
Mutagenesisi182 – 1821C → A: Loss the capacity to oxidize thioredoxin; when associated with A-177. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 191191FAD-linked sulfhydryl oxidase ERV1PRO_0000401377Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi119 ↔ 122Redox-activePROSITE-ProRule annotation
Disulfide bondi148 ↔ 165PROSITE-ProRule annotation1 Publication
Disulfide bondi177 ↔ 182PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ8GXX0.
PRIDEiQ8GXX0.

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

STRINGi3702.AT1G49880.1.

Structurei

Secondary structure

1
191
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi76 – 9318Combined sources
Helixi100 – 11617Combined sources
Helixi120 – 13213Combined sources
Helixi140 – 15718Combined sources
Helixi168 – 1714Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HJ3X-ray2.50A/B71-191[»]
ProteinModelPortaliQ8GXX0.
SMRiQ8GXX0. Positions 73-173.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8GXX0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini72 – 172101ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi177 – 1826Required for dimerization and substrate specificity

Sequence similaritiesi

Contains 1 ERV/ALR sulfhydryl oxidase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5054.
HOGENOMiHOG000120834.
InParanoidiQ8GXX0.
KOiK17783.
OMAiPCRECAD.
PhylomeDBiQ8GXX0.

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
[Graphical view]
PfamiPF04777. Evr1_Alr. 1 hit.
[Graphical view]
SUPFAMiSSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8GXX0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEKPWQPLL QSFEKLSNCV QTHLSNFIGI KNTPPSSQST IQNPIISLDS
60 70 80 90 100
SPPIATNSSS LQKLPLKDKS TGPVTKEDLG RATWTFLHTL AAQYPEKPTR
110 120 130 140 150
QQKKDVKELM TILSRMYPCR ECADHFKEIL RSNPAQAGSQ EEFSQWLCHV
160 170 180 190
HNTVNRSLGK LVFPCERVDA RWGKLECEQK SCDLHGTSMD F
Length:191
Mass (Da):21,600
Last modified:March 1, 2003 - v1
Checksum:i7CC7FEA44C0D4B38
GO

Sequence cautioni

The sequence AAG51780.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti35 – 362PS → R in AAM63908 (Ref. 6) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ551263 mRNA. Translation: CAD83013.1.
AC079674 Genomic DNA. Translation: AAG51780.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32487.1.
AK117991 mRNA. Translation: BAC42626.1.
BT003718 mRNA. Translation: AAO39946.1.
AY086861 mRNA. Translation: AAM63908.1.
PIRiG96535.
RefSeqiNP_564557.1. NM_103875.3.
UniGeneiAt.38141.

Genome annotation databases

EnsemblPlantsiAT1G49880.1; AT1G49880.1; AT1G49880.
GeneIDi841411.
KEGGiath:AT1G49880.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ551263 mRNA. Translation: CAD83013.1.
AC079674 Genomic DNA. Translation: AAG51780.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32487.1.
AK117991 mRNA. Translation: BAC42626.1.
BT003718 mRNA. Translation: AAO39946.1.
AY086861 mRNA. Translation: AAM63908.1.
PIRiG96535.
RefSeqiNP_564557.1. NM_103875.3.
UniGeneiAt.38141.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HJ3X-ray2.50A/B71-191[»]
ProteinModelPortaliQ8GXX0.
SMRiQ8GXX0. Positions 73-173.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G49880.1.

Proteomic databases

PaxDbiQ8GXX0.
PRIDEiQ8GXX0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G49880.1; AT1G49880.1; AT1G49880.
GeneIDi841411.
KEGGiath:AT1G49880.

Organism-specific databases

TAIRiAT1G49880.

Phylogenomic databases

eggNOGiCOG5054.
HOGENOMiHOG000120834.
InParanoidiQ8GXX0.
KOiK17783.
OMAiPCRECAD.
PhylomeDBiQ8GXX0.

Enzyme and pathway databases

BioCyciARA:AT1G49880-MONOMER.
BRENDAi1.8.3.2. 399.

Miscellaneous databases

EvolutionaryTraceiQ8GXX0.
PROiQ8GXX0.

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
[Graphical view]
PfamiPF04777. Evr1_Alr. 1 hit.
[Graphical view]
SUPFAMiSSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Unique features of plant mitochondrial sulfhydryl oxidase."
    Levitan A., Danon A., Lisowsky T.
    J. Biol. Chem. 279:20002-20008(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "Gain of function in an ERV/ALR sulfhydryl oxidase by molecular engineering of the shuttle disulfide."
    Vitu E., Bentzur M., Lisowsky T., Kaiser C.A., Fass D.
    J. Mol. Biol. 362:89-101(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 71-191 IN COMPLEX WITH FAD, SUBUNIT, DISULFIDE BONDS, MUTAGENESIS OF CYS-177 AND CYS-182.

Entry informationi

Entry nameiERV1_ARATH
AccessioniPrimary (citable) accession number: Q8GXX0
Secondary accession number(s): Q8LC15, Q9C6C6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: March 1, 2003
Last modified: June 24, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.