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Q8GXW5

- GLN15_ARATH

UniProt

Q8GXW5 - GLN15_ARATH

Protein

Glutamine synthetase cytosolic isozyme 1-5

Gene

GLN1-5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro
    2. nitrogen fixation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Nitrogen fixation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase cytosolic isozyme 1-5 (EC:6.3.1.2)
    Alternative name(s):
    Glutamate--ammonia ligase GLN1;5
    Short name:
    GLN1;5
    Gene namesi
    Name:GLN1-5
    Ordered Locus Names:At1g48470
    ORF Names:T1N15.8, T1N15_7
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G48470.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. plasmodesma Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 353352Glutamine synthetase cytosolic isozyme 1-5PRO_0000239821Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylthreonine1 Publication
    Modified residuei3 – 31PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ8GXW5.
    PRIDEiQ8GXW5.

    Expressioni

    Tissue specificityi

    Not expressed in roots.1 Publication

    Gene expression databases

    ArrayExpressiQ8GXW5.
    GenevestigatoriQ8GXW5.

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Protein-protein interaction databases

    BioGridi26493. 1 interaction.
    IntActiQ8GXW5. 1 interaction.
    STRINGi3702.AT1G48470.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8GXW5.
    SMRiQ8GXW5. Positions 5-353.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Phylogenomic databases

    eggNOGiCOG0174.
    HOGENOMiHOG000061500.
    InParanoidiQ8GXW5.
    KOiK01915.
    OMAiRSKTKIL.
    PhylomeDBiQ8GXW5.

    Family and domain databases

    Gene3Di3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8GXW5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSPLSDLLN LDLSDTKKII AEYIWIGGSG MDIRSKARTL PGPVSNPTKL    50
    PKWNYDGSST DQAAGDDSEV ILYPQAIFKD PFRKGNNILV MCDAYRPAGD 100
    PIPTNNRHKA VKIFDHPNVK AEEPWFGIEQ EYTLLKKDVK WPLGWPLGGF 150
    PGPQGPYYCA VGADKAFGRD IVDAHYKACL YSGLSIGGAN GEVMPGQWEF 200
    QISPTVGIGA GDQLWVARYI LERITEICGV IVSFDPKPIQ GDWNGAAAHT 250
    NFSTKSMRKD GGLDLIKEAI KKLEVKHKQH IAAYGEGNER RLTGKHETAD 300
    INTFSWGVAD RGASVRVGRD TEKEGKGYFE DRRPSSNMDP YLVTSMIAET 350
    TIL 353
    Length:353
    Mass (Da):38,907
    Last modified:March 1, 2003 - v1
    Checksum:i79C4A429340C697B
    GO

    Sequence cautioni

    The sequence AAF79695.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti46 – 461N → D in AAM62764. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC020889 Genomic DNA. Translation: AAF79695.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE32297.1.
    AK118005 mRNA. Translation: BAC42638.1.
    BT006245 mRNA. Translation: AAP12894.1.
    AY085540 mRNA. Translation: AAM62764.1.
    RefSeqiNP_175280.1. NM_103743.2.
    UniGeneiAt.28083.

    Genome annotation databases

    EnsemblPlantsiAT1G48470.1; AT1G48470.1; AT1G48470.
    GeneIDi841268.
    KEGGiath:AT1G48470.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC020889 Genomic DNA. Translation: AAF79695.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE32297.1 .
    AK118005 mRNA. Translation: BAC42638.1 .
    BT006245 mRNA. Translation: AAP12894.1 .
    AY085540 mRNA. Translation: AAM62764.1 .
    RefSeqi NP_175280.1. NM_103743.2.
    UniGenei At.28083.

    3D structure databases

    ProteinModelPortali Q8GXW5.
    SMRi Q8GXW5. Positions 5-353.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 26493. 1 interaction.
    IntActi Q8GXW5. 1 interaction.
    STRINGi 3702.AT1G48470.1-P.

    Proteomic databases

    PaxDbi Q8GXW5.
    PRIDEi Q8GXW5.

    Protocols and materials databases

    DNASUi 841268.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G48470.1 ; AT1G48470.1 ; AT1G48470 .
    GeneIDi 841268.
    KEGGi ath:AT1G48470.

    Organism-specific databases

    TAIRi AT1G48470.

    Phylogenomic databases

    eggNOGi COG0174.
    HOGENOMi HOG000061500.
    InParanoidi Q8GXW5.
    KOi K01915.
    OMAi RSKTKIL.
    PhylomeDBi Q8GXW5.

    Gene expression databases

    ArrayExpressi Q8GXW5.
    Genevestigatori Q8GXW5.

    Family and domain databases

    Gene3Di 3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "Kinetic properties and ammonium-dependent regulation of cytosolic isoenzymes of glutamine synthetase in Arabidopsis."
      Ishiyama K., Inoue E., Watanabe-Takahashi A., Obara M., Yamaya T., Takahashi H.
      J. Biol. Chem. 279:16598-16605(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    7. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGLN15_ARATH
    AccessioniPrimary (citable) accession number: Q8GXW5
    Secondary accession number(s): Q8LEA1, Q9LP78
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 13, 2006
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3