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Q8GXW5

- GLN15_ARATH

UniProt

Q8GXW5 - GLN15_ARATH

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Protein

Glutamine synthetase cytosolic isozyme 1-5

Gene

GLN1-5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
  2. nitrogen fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_232680. Amino acid synthesis and interconversion (transamination).
REACT_262794. Astrocytic Glutamate-Glutamine Uptake And Metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase cytosolic isozyme 1-5 (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase GLN1;5
Short name:
GLN1;5
Gene namesi
Name:GLN1-5
Ordered Locus Names:At1g48470
ORF Names:T1N15.8, T1N15_7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G48470.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 353352Glutamine synthetase cytosolic isozyme 1-5PRO_0000239821Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonine1 Publication
Modified residuei3 – 31PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8GXW5.
PRIDEiQ8GXW5.

Expressioni

Tissue specificityi

Not expressed in roots.1 Publication

Gene expression databases

ExpressionAtlasiQ8GXW5. baseline and differential.
GenevestigatoriQ8GXW5.

Interactioni

Subunit structurei

Homooctamer.By similarity

Protein-protein interaction databases

BioGridi26493. 1 interaction.
IntActiQ8GXW5. 1 interaction.
STRINGi3702.AT1G48470.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8GXW5.
SMRiQ8GXW5. Positions 5-353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiCOG0174.
HOGENOMiHOG000061500.
InParanoidiQ8GXW5.
KOiK01915.
OMAiRSKTKIL.
PhylomeDBiQ8GXW5.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8GXW5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSPLSDLLN LDLSDTKKII AEYIWIGGSG MDIRSKARTL PGPVSNPTKL
60 70 80 90 100
PKWNYDGSST DQAAGDDSEV ILYPQAIFKD PFRKGNNILV MCDAYRPAGD
110 120 130 140 150
PIPTNNRHKA VKIFDHPNVK AEEPWFGIEQ EYTLLKKDVK WPLGWPLGGF
160 170 180 190 200
PGPQGPYYCA VGADKAFGRD IVDAHYKACL YSGLSIGGAN GEVMPGQWEF
210 220 230 240 250
QISPTVGIGA GDQLWVARYI LERITEICGV IVSFDPKPIQ GDWNGAAAHT
260 270 280 290 300
NFSTKSMRKD GGLDLIKEAI KKLEVKHKQH IAAYGEGNER RLTGKHETAD
310 320 330 340 350
INTFSWGVAD RGASVRVGRD TEKEGKGYFE DRRPSSNMDP YLVTSMIAET

TIL
Length:353
Mass (Da):38,907
Last modified:March 1, 2003 - v1
Checksum:i79C4A429340C697B
GO

Sequence cautioni

The sequence AAF79695.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti46 – 461N → D in AAM62764. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC020889 Genomic DNA. Translation: AAF79695.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32297.1.
AK118005 mRNA. Translation: BAC42638.1.
BT006245 mRNA. Translation: AAP12894.1.
AY085540 mRNA. Translation: AAM62764.1.
RefSeqiNP_175280.1. NM_103743.2.
UniGeneiAt.28083.

Genome annotation databases

EnsemblPlantsiAT1G48470.1; AT1G48470.1; AT1G48470.
GeneIDi841268.
KEGGiath:AT1G48470.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC020889 Genomic DNA. Translation: AAF79695.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE32297.1 .
AK118005 mRNA. Translation: BAC42638.1 .
BT006245 mRNA. Translation: AAP12894.1 .
AY085540 mRNA. Translation: AAM62764.1 .
RefSeqi NP_175280.1. NM_103743.2.
UniGenei At.28083.

3D structure databases

ProteinModelPortali Q8GXW5.
SMRi Q8GXW5. Positions 5-353.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 26493. 1 interaction.
IntActi Q8GXW5. 1 interaction.
STRINGi 3702.AT1G48470.1-P.

Proteomic databases

PaxDbi Q8GXW5.
PRIDEi Q8GXW5.

Protocols and materials databases

DNASUi 841268.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G48470.1 ; AT1G48470.1 ; AT1G48470 .
GeneIDi 841268.
KEGGi ath:AT1G48470.

Organism-specific databases

TAIRi AT1G48470.

Phylogenomic databases

eggNOGi COG0174.
HOGENOMi HOG000061500.
InParanoidi Q8GXW5.
KOi K01915.
OMAi RSKTKIL.
PhylomeDBi Q8GXW5.

Enzyme and pathway databases

Reactomei REACT_232680. Amino acid synthesis and interconversion (transamination).
REACT_262794. Astrocytic Glutamate-Glutamine Uptake And Metabolism.

Gene expression databases

ExpressionAtlasi Q8GXW5. baseline and differential.
Genevestigatori Q8GXW5.

Family and domain databases

Gene3Di 3.30.590.10. 1 hit.
InterProi IPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view ]
Pfami PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF54368. SSF54368. 1 hit.
PROSITEi PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Kinetic properties and ammonium-dependent regulation of cytosolic isoenzymes of glutamine synthetase in Arabidopsis."
    Ishiyama K., Inoue E., Watanabe-Takahashi A., Obara M., Yamaya T., Takahashi H.
    J. Biol. Chem. 279:16598-16605(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGLN15_ARATH
AccessioniPrimary (citable) accession number: Q8GXW5
Secondary accession number(s): Q8LEA1, Q9LP78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: March 1, 2003
Last modified: November 26, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3