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Protein

DELLA protein RGL2

Gene

RGL2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. No effect of the BOI proteins on its stability. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Upon GA application, it is degraded by the proteasome, allowing the GA signaling pathway. Acts as a major GA-response repressor of seed germination, including seed thermoinhibition. Promotes the biosynthesis of abscisic acid (ABA), especially in seed coats to maintain seed dormancy. Delays flowering and adult leaf production. Also regulates the floral development, petal, stamen and anther development, by repressing the continued growth of floral organs. Its activity is probably regulated by other phytohormones such as auxin and ethylene. Involved in the regulation of seed dormancy and germination, including glucose-induced delay of seed germination. Promotes salt tolerance. Acts as a repressor of positive regulators of trichome initiation. Required during the flagellin-derived peptide flg22-mediated growth inhibition. Contributes to the susceptibility to the biotrophic pathogen P.syringae pv. tomato and to the resistance to the necrotrophic pathogens B.cinerea A.brassicicola, probably by repressing the SA-defense pathway and preventing cell death. Prevents stress-induced reactive oxygen species (ROS) accumulation (e.g. salt stress) by acting on the ROS scavenging system, and delays ROS-induced cell death, thus promoting stress tolerance.17 Publications

GO - Molecular functioni

  • DNA binding transcription factor activity Source: TAIR
  • sequence-specific DNA binding Source: TAIR
  • transcription factor activity, transcription factor binding Source: InterPro

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • defense response Source: UniProtKB-KW
  • flower development Source: UniProtKB-KW
  • gibberellic acid mediated signaling pathway Source: TAIR
  • negative regulation of gibberellic acid mediated signaling pathway Source: TAIR
  • negative regulation of seed germination Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to gibberellin Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein, Repressor
Biological processDifferentiation, Flowering, Gibberellin signaling pathway, Plant defense, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
DELLA protein RGL2
Alternative name(s):
GRAS family protein 15
Short name:
AtGRAS-15
RGA-like protein 2
Scarecrow-like protein 19
Short name:
AtSCL19
Gene namesi
Name:RGL2
Synonyms:SCL19
Ordered Locus Names:At3g03450
ORF Names:T21P5.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G03450
TAIRilocus:2099624 AT3G03450

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Higher germination rate in the presence of glucose and at supraoptimal temperature conditions. Rga, gai, rgl1, rgl2 and rgl3 pentuple mutant displays constitutive GA responses even in the absence of GA treatment.3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi44 – 48Missing : Resistant to GA-mediated degradation. 1 Publication5
Mutagenesisi52Y → A or E: Resistant to GA-mediated degradation. 2 Publications1
Mutagenesisi52Y → F: No effect on GA-mediated degradation. 2 Publications1
Mutagenesisi57S → D: No effect on GA-mediated degradation. 1 Publication1
Mutagenesisi86S → D: No effect on GA-mediated degradation. 1 Publication1
Mutagenesisi89Y → A or E: Resistant to GA-mediated degradation. 1 Publication1
Mutagenesisi89Y → F: No effect on GA-mediated degradation. 1 Publication1
Mutagenesisi103S → D: No effect on GA-mediated degradation. 1 Publication1
Mutagenesisi212S → D: No effect on GA-mediated degradation. 1 Publication1
Mutagenesisi223Y → A or E: Resistant to GA-mediated degradation. 1 Publication1
Mutagenesisi223Y → F: No effect on GA-mediated degradation. 1 Publication1
Mutagenesisi259Y → A: Partially resistant to GA-mediated degradation. 1 Publication1
Mutagenesisi271T → C: Partially resistant to GA-mediated degradation. 1 Publication1
Mutagenesisi271T → E: Null mutant; resistant to GA-mediated degradation. 1 Publication1
Mutagenesisi319T → C: No effect on GA-mediated degradation. 1 Publication1
Mutagenesisi319T → E: Resistant to GA-mediated degradation. 1 Publication1
Mutagenesisi381S → D: No effect on GA-mediated degradation. 1 Publication1
Mutagenesisi411T → C: No effect on GA-mediated degradation. 1 Publication1
Mutagenesisi411T → E: Resistant to GA-mediated degradation. 1 Publication1
Mutagenesisi435Y → A or E: Resistant to GA-mediated degradation. 1 Publication1
Mutagenesisi435Y → F: No effect on GA-mediated degradation. 1 Publication1
Mutagenesisi436S → D: No effect on GA-mediated degradation. 1 Publication1
Mutagenesisi437S → D: No effect on GA-mediated degradation. 1 Publication1
Mutagenesisi441S → C: No effect on GA-mediated degradation. 1 Publication1
Mutagenesisi441S → D: Resistant to GA-mediated degradation. 1 Publication1
Mutagenesisi456S → D: No effect on GA-mediated degradation. 1 Publication1
Mutagenesisi481T → E: No effect on GA-mediated degradation. 1 Publication1
Mutagenesisi501S → D: No effect on GA-mediated degradation. 1 Publication1
Mutagenesisi508S → D: No effect on GA-mediated degradation. 1 Publication1
Mutagenesisi535T → C: No effect on GA-mediated degradation. 1 Publication1
Mutagenesisi535T → E: Resistant to GA-mediated degradation. 1 Publication1
Mutagenesisi542S → C: No effect on GA-mediated degradation. 1 Publication1
Mutagenesisi542S → D: Resistant to GA-mediated degradation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001322371 – 547DELLA protein RGL2Add BLAST547

Post-translational modificationi

Phosphorylated. Phosphorylation on Tyr residues is required for proteasome-mediated degradation in response to gibberellic acid (GA). Dephosphorylation may be prerequisite for its degradation by the proteasome.2 Publications
Ubiquitinated (Probable). Upon GA application or seed imbibation, it is ubiquitinated by the SCF(GID2) complex, leading to its subsequent degradation.Curated

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8GXW1

Expressioni

Tissue specificityi

Predominantly expressed in germinating seeds, flowers and siliques. Only detectable in the inflorescence, with high levels in young flower buds and significant levels in siliques. In imbibed seeds, it is restricted to seed coats, elongating regions of pre-emergent and recently emerged radicles, endosperm (especially micropylar endosperm), and embryonic axis. Not expressed in leaves, bolting stems or roots.5 Publications

Inductioni

Up-regulated transiently following seed imbibition to decline rapidly as germination proceeds; this induction is delayed at supraoptimal temperature conditions (e.g. 34 degrees Celsius). Accumulates in seed coats of dormant seeds where germination does not occur after imbibition. Increased levels upon abscisic acid (ABA) treatment. Down-regulated by norflurazon (NF), an ABA biosynthesis inhibitor. Induced by stress such as glucose, salt or mannitol treatment.7 Publications

Gene expression databases

ExpressionAtlasiQ8GXW1 baseline and differential
GenevisibleiQ8GXW1 AT

Interactioni

Subunit structurei

Interacts directly with the GID2/SLY1 component of the SCF(GID2) complex. Interacts (via N-terminus) with GID1A, GID1B and GID1C (via N-terminus). Binds to bHLH transcription factors such as PIF1, PIF4, PIF6 and SPT. Interacts with the BOI proteins BOI, BRG1, BRG2 and BRG3.5 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi6584, 18 interactors
DIPiDIP-37661N
IntActiQ8GXW1, 5 interactors
STRINGi3702.AT3G03450.1

Structurei

3D structure databases

ProteinModelPortaliQ8GXW1
SMRiQ8GXW1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini171 – 545GRASPROSITE-ProRule annotationAdd BLAST375

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni178 – 236Leucine repeat I (LRI)PROSITE-ProRule annotationAdd BLAST59
Regioni255 – 320VHIIDPROSITE-ProRule annotationAdd BLAST66
Regioni334 – 366Leucine repeat II (LRII)PROSITE-ProRule annotationAdd BLAST33
Regioni377 – 466PFYREPROSITE-ProRule annotationAdd BLAST90
Regioni469 – 545SAWPROSITE-ProRule annotationAdd BLAST77

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi44 – 48DELLA motif5
Motifi66 – 70LEXLE motif5
Motifi87 – 91VHYNP motif5
Motifi286 – 290VHIIDPROSITE-ProRule annotation5
Motifi385 – 389LXXLL motifPROSITE-ProRule annotation5

Domaini

The DELLA motif is required for its GA-induced degradation but not for the interaction with GID2.

Sequence similaritiesi

Belongs to the GRAS family. DELLA subfamily.Curated

Phylogenomic databases

eggNOGiENOG410IQYH Eukaryota
ENOG410YBFY LUCA
HOGENOMiHOG000238577
InParanoidiQ8GXW1
KOiK14494
OMAiVLQMHFY
OrthoDBiEOG0936075R
PhylomeDBiQ8GXW1

Family and domain databases

Gene3Di1.10.10.1290, 1 hit
InterProiView protein in InterPro
IPR038088 DELLA_N_sf
IPR030006 TF_DELLA
IPR021914 TF_DELLA_N
IPR005202 TF_GRAS
PANTHERiPTHR31636:SF47 PTHR31636:SF47, 1 hit
PfamiView protein in Pfam
PF12041 DELLA, 1 hit
PF03514 GRAS, 1 hit
PROSITEiView protein in PROSITE
PS50985 GRAS, 1 hit

Sequencei

Sequence statusi: Complete.

Q8GXW1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRGYGETWD PPPKPLPASR SGEGPSMADK KKADDDNNNS NMDDELLAVL
60 70 80 90 100
GYKVRSSEMA EVAQKLEQLE MVLSNDDVGS TVLNDSVHYN PSDLSNWVES
110 120 130 140 150
MLSELNNPAS SDLDTTRSCV DRSEYDLRAI PGLSAFPKEE EVFDEEASSK
160 170 180 190 200
RIRLGSWCES SDESTRSVVL VDSQETGVRL VHALVACAEA IHQENLNLAD
210 220 230 240 250
ALVKRVGTLA GSQAGAMGKV ATYFAQALAR RIYRDYTAET DVCAAVNPSF
260 270 280 290 300
EEVLEMHFYE SCPYLKFAHF TANQAILEAV TTARRVHVID LGLNQGMQWP
310 320 330 340 350
ALMQALALRP GGPPSFRLTG IGPPQTENSD SLQQLGWKLA QFAQNMGVEF
360 370 380 390 400
EFKGLAAESL SDLEPEMFET RPESETLVVN SVFELHRLLA RSGSIEKLLN
410 420 430 440 450
TVKAIKPSIV TVVEQEANHN GIVFLDRFNE ALHYYSSLFD SLEDSYSLPS
460 470 480 490 500
QDRVMSEVYL GRQILNVVAA EGSDRVERHE TAAQWRIRMK SAGFDPIHLG
510 520 530 540
SSAFKQASML LSLYATGDGY RVEENDGCLM IGWQTRPLIT TSAWKLA
Length:547
Mass (Da):60,494
Last modified:November 22, 2005 - v2
Checksum:iC4D18D5951D95634
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti210A → T in BAC42642 (PubMed:11910074).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009895 Genomic DNA Translation: AAF01590.1
CP002686 Genomic DNA Translation: AEE73945.1
AK118009 mRNA Translation: BAC42642.1
RefSeqiNP_186995.1, NM_111216.3
UniGeneiAt.18489
At.67027

Genome annotation databases

EnsemblPlantsiAT3G03450.1; AT3G03450.1; AT3G03450
GeneIDi821251
GrameneiAT3G03450.1; AT3G03450.1; AT3G03450
KEGGiath:AT3G03450

Similar proteinsi

Entry informationi

Entry nameiRGL2_ARATH
AccessioniPrimary (citable) accession number: Q8GXW1
Secondary accession number(s): Q9SRP9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: April 25, 2018
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health