Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

(S)-ureidoglycine aminohydrolase

Gene

UGLYAH

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the catabolism of purine nucleotides. Can use (S)-2-ureidoglycine as substrate, but not allantoate. The sequential activity of AAH, UGLYAH and UAH allows a complete purine breakdown without the intermediate generation of urea.3 Publications

Catalytic activityi

(S)-2-ureidoglycine + H2O = (S)-ureidoglycolate + NH3.3 Publications

Cofactori

Mn2+1 Publication

Kineticsi

kcat is 761 sec(-1) for (S)-2-ureidoglycine.

  1. KM=1.77 mM for (S)-2-ureidoglycine1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi235Manganese1 Publication1
    Binding sitei235Substrate1 Publication1
    Metal bindingi237Manganese1 Publication1
    Metal bindingi241Manganese1 Publication1
    Metal bindingi275Manganese1 Publication1
    Binding sitei275Substrate1 Publication1
    Binding sitei287Substrate1 Publication1
    Binding sitei291Substrate1 Publication1

    GO - Molecular functioni

    • metal ion binding Source: UniProtKB-KW
    • transcription factor activity, sequence-specific DNA binding Source: TAIR
    • ureidoglycine aminohydrolase activity Source: TAIR

    GO - Biological processi

    • allantoin catabolic process Source: TAIR
    • purine nucleobase catabolic process Source: TAIR
    • regulation of transcription, DNA-templated Source: TAIR
    • ureide catabolic process Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Purine metabolism

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BRENDAi3.5.3.26. 399.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    (S)-ureidoglycine aminohydrolase (EC:3.5.3.26)
    Short name:
    AtUGLYAH
    Gene namesi
    Name:UGLYAH
    Synonyms:UGHY, YlbA
    Ordered Locus Names:At4g17050
    ORF Names:dl4555w, FCAALL.343
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 4

    Organism-specific databases

    TAIRiAT4G17050.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Endoplasmic reticulum

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi221H → A: Decreased activity. 1 Publication1
    Mutagenesisi235E → A: Loss of manganese binding and loss of activity. 1 Publication1
    Mutagenesisi235E → Q: No effect on manganese binding, but loss of activity. 1 Publication1
    Mutagenesisi237H → A: Loss of activity. 1 Publication1
    Mutagenesisi241H → A: Loss of activity. 1 Publication1
    Mutagenesisi252Y → F: No effect on the affinity for the substrate, but decreased activity. 1 Publication1
    Mutagenesisi275Q → A: Loss of activity. 1 Publication1
    Mutagenesisi287Y → A or F: Loss of activity. 1 Publication1
    Mutagenesisi291K → A: Loss of activity. 1 Publication1
    Mutagenesisi291K → R: Increased affinity for the substrate, but decreased activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 20Sequence analysisAdd BLAST20
    ChainiPRO_000042344421 – 298(S)-ureidoglycine aminohydrolaseAdd BLAST278

    Proteomic databases

    PaxDbiQ8GXV5.

    PTM databases

    iPTMnetiQ8GXV5.

    Interactioni

    Subunit structurei

    Homooctamer.1 Publication

    Protein-protein interaction databases

    STRINGi3702.AT4G17050.1.

    Structurei

    Secondary structure

    1298
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi42 – 44Combined sources3
    Helixi51 – 54Combined sources4
    Beta strandi67 – 72Combined sources6
    Helixi74 – 76Combined sources3
    Beta strandi85 – 94Combined sources10
    Helixi96 – 98Combined sources3
    Beta strandi101 – 109Combined sources9
    Beta strandi111 – 114Combined sources4
    Beta strandi122 – 131Combined sources10
    Beta strandi133 – 136Combined sources4
    Beta strandi143 – 145Combined sources3
    Beta strandi149 – 153Combined sources5
    Beta strandi160 – 165Combined sources6
    Beta strandi167 – 175Combined sources9
    Beta strandi187 – 190Combined sources4
    Helixi191 – 193Combined sources3
    Beta strandi204 – 210Combined sources7
    Beta strandi217 – 225Combined sources9
    Beta strandi241 – 247Combined sources7
    Beta strandi249 – 254Combined sources6
    Beta strandi257 – 262Combined sources6
    Beta strandi266 – 269Combined sources4
    Beta strandi275 – 283Combined sources9
    Beta strandi285 – 292Combined sources8

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4E2QX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P36-298[»]
    4E2SX-ray2.59A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P36-298[»]
    ProteinModelPortaliQ8GXV5.
    SMRiQ8GXV5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi139 – 142Poly-Ser4
    Compositional biasi243 – 246Poly-Leu4

    Sequence similaritiesi

    Belongs to the UGHY family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG410IJQM. Eukaryota.
    COG3257. LUCA.
    HOGENOMiHOG000219622.
    InParanoidiQ8GXV5.
    KOiK14977.
    OMAiRFIFVVQ.
    OrthoDBiEOG09360HKR.
    PhylomeDBiQ8GXV5.

    Family and domain databases

    Gene3Di2.60.120.10. 1 hit.
    InterProiIPR013096. Cupin_2.
    IPR014710. RmlC-like_jellyroll.
    IPR011051. RmlC_Cupin.
    [Graphical view]
    PfamiPF07883. Cupin_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF51182. SSF51182. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8GXV5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRSLYLIVFI VISLVKASKS DDGFCSAPSI VESDEKTNPI YWKATNPTLS
    60 70 80 90 100
    PSHLQDLPGF TRSVYKRDHA LITPESHVYS PLPDWTNTLG AYLITPATGS
    110 120 130 140 150
    HFVMYLAKMK EMSSSGLPPQ DIERLIFVVE GAVTLTNTSS SSKKLTVDSY
    160 170 180 190 200
    AYLPPNFHHS LDCVESATLV VFERRYEYLG SHTTELIVGS TDKQPLLETP
    210 220 230 240 250
    GEVFELRKLL PMSVAYDFNI HTMDFQPGEF LNVKEVHYNQ HGLLLLEGQG
    260 270 280 290
    IYRLGDNWYP VQAGDVIWMA PFVPQWYAAL GKTRSRYLLY KDVNRNPL
    Length:298
    Mass (Da):33,673
    Last modified:March 1, 2003 - v1
    Checksum:i50CED7DBA1C40A99
    GO

    Sequence cautioni

    The sequence CAB10485 differs from that shown. Reason: Erroneous gene model prediction.Curated
    The sequence CAB80976 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti138T → S in BAD44550 (Ref. 6) Curated1
    Sequence conflicti224D → G in BAD43635 (Ref. 6) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    GQ303359 mRNA. Translation: ADH04164.1.
    Z97342 Genomic DNA. Translation: CAB10485.1. Sequence problems.
    AL161545 Genomic DNA. Translation: CAB80976.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE83843.1.
    AK118019 mRNA. Translation: BAC42652.1.
    AK176787 mRNA. Translation: BAD44550.1.
    AK175872 mRNA. Translation: BAD43635.1.
    PIRiH71438.
    RefSeqiNP_193438.2. NM_117809.6.
    UniGeneiAt.44735.

    Genome annotation databases

    EnsemblPlantsiAT4G17050.1; AT4G17050.1; AT4G17050.
    GeneIDi827413.
    GrameneiAT4G17050.1; AT4G17050.1; AT4G17050.
    KEGGiath:AT4G17050.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    GQ303359 mRNA. Translation: ADH04164.1.
    Z97342 Genomic DNA. Translation: CAB10485.1. Sequence problems.
    AL161545 Genomic DNA. Translation: CAB80976.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE83843.1.
    AK118019 mRNA. Translation: BAC42652.1.
    AK176787 mRNA. Translation: BAD44550.1.
    AK175872 mRNA. Translation: BAD43635.1.
    PIRiH71438.
    RefSeqiNP_193438.2. NM_117809.6.
    UniGeneiAt.44735.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4E2QX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P36-298[»]
    4E2SX-ray2.59A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P36-298[»]
    ProteinModelPortaliQ8GXV5.
    SMRiQ8GXV5.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT4G17050.1.

    PTM databases

    iPTMnetiQ8GXV5.

    Proteomic databases

    PaxDbiQ8GXV5.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT4G17050.1; AT4G17050.1; AT4G17050.
    GeneIDi827413.
    GrameneiAT4G17050.1; AT4G17050.1; AT4G17050.
    KEGGiath:AT4G17050.

    Organism-specific databases

    TAIRiAT4G17050.

    Phylogenomic databases

    eggNOGiENOG410IJQM. Eukaryota.
    COG3257. LUCA.
    HOGENOMiHOG000219622.
    InParanoidiQ8GXV5.
    KOiK14977.
    OMAiRFIFVVQ.
    OrthoDBiEOG09360HKR.
    PhylomeDBiQ8GXV5.

    Enzyme and pathway databases

    BRENDAi3.5.3.26. 399.

    Miscellaneous databases

    PROiQ8GXV5.

    Family and domain databases

    Gene3Di2.60.120.10. 1 hit.
    InterProiIPR013096. Cupin_2.
    IPR014710. RmlC-like_jellyroll.
    IPR011051. RmlC_Cupin.
    [Graphical view]
    PfamiPF07883. Cupin_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF51182. SSF51182. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiUGHY_ARATH
    AccessioniPrimary (citable) accession number: Q8GXV5
    Secondary accession number(s): O23549, Q67XN1, Q680J5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 18, 2013
    Last sequence update: March 1, 2003
    Last modified: November 30, 2016
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.