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Protein

1-acyl-sn-glycerol-3-phosphate acyltransferase 1, chloroplastic

Gene

LPAT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating acyl moiety at the 2 position. Has preference for C-16-CoA substrates compared to C-18-CoA substrates. Essential for embryo development during the transition from the globular to the heart stage when chloroplasts begin to form.2 Publications

Catalytic activityi

Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.2 Publications

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase, chloroplastic (ATS1), Glycerol-3-phosphate acyltransferase 1 (GPAT1), Probable glycerol-3-phosphate acyltransferase 3 (GPAT3), Glycerol-3-phosphate acyltransferase 5 (GPAT5), Glycerol-3-phosphate acyltransferase, chloroplastic (AXX17_At1g32940), Probable glycerol-3-phosphate acyltransferase 2 (GPAT2), Glycerol-3-phosphate acyltransferase 7 (GPAT7), Probable glycerol-3-phosphate acyltransferase 8 (GPAT8)
  2. 1-acyl-sn-glycerol-3-phosphate acyltransferase 3 (LPAT3), 1-acyl-sn-glycerol-3-phosphate acyltransferase 1, chloroplastic (LPAT1), Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 (LPAT5), Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 4 (LPAT4), 1-acyl-sn-glycerol-3-phosphate acyltransferase 2 (LPAT2)
  3. Phosphatidate cytidylyltransferase 1 (CDS1), Phosphatidate cytidylyltransferase (AXX17_At1g55730), Phosphatidate cytidylyltransferase 2 (CDS2), Phosphatidate cytidylyltransferase (AXX17_At2g42720), Phosphatidate cytidylyltransferase (AXX17_At4g30920), Phosphatidate cytidylyltransferase (CDS2), Phosphatidate cytidylyltransferase (CDS2), Phosphatidate cytidylyltransferase 3 (CDS3), Phosphatidate cytidylyltransferase 4, chloroplastic (CDS4), Phosphatidate cytidylyltransferase (AXX17_At3g55010), Phosphatidate cytidylyltransferase (AXX17_At4g25970), Phosphatidate cytidylyltransferase 5, chloroplastic (CDS5)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

  • CDP-diacylglycerol biosynthetic process Source: UniProtKB-UniPathway
  • embryo development ending in seed dormancy Source: TAIR
  • phosphatidylglycerol biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Developmental protein, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:AT4G30580-MONOMER.
BRENDAi2.3.1.51. 399.
ReactomeiR-ATH-1483166. Synthesis of PA.
R-ATH-163765. ChREBP activates metabolic gene expression.
R-ATH-6798695. Neutrophil degranulation.
R-ATH-75109. Triglyceride Biosynthesis.
UniPathwayiUPA00557; UER00613.

Names & Taxonomyi

Protein namesi
Recommended name:
1-acyl-sn-glycerol-3-phosphate acyltransferase 1, chloroplastic (EC:2.3.1.51)
Alternative name(s):
Lysophosphatidyl acyltransferase 1
Protein EMBRYO DEFECTIVE 1995
Gene namesi
Name:LPAT1
Synonyms:ATS2, EMB1995, LPAAT1
Ordered Locus Names:At4g30580
ORF Names:F17I23.80
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G30580.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei127 – 147HelicalSequence analysisAdd BLAST21
Transmembranei224 – 244HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 56ChloroplastSequence analysisAdd BLAST56
ChainiPRO_000002470157 – 3561-acyl-sn-glycerol-3-phosphate acyltransferase 1, chloroplasticAdd BLAST300

Proteomic databases

PaxDbiQ8GXU8.

Expressioni

Tissue specificityi

Widely expressed. Expressed at higher level in leaves. Expressed at lower level in silique walls compared to leaves.2 Publications

Developmental stagei

Expression transiently increases in siliques 4 hours after flowering.1 Publication

Gene expression databases

GenevisibleiQ8GXU8. AT.

Interactioni

Protein-protein interaction databases

BioGridi14468. 1 interactor.
STRINGi3702.AT4G30580.1.

Structurei

3D structure databases

ProteinModelPortaliQ8GXU8.
SMRiQ8GXU8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi202 – 207HXXXXD motif6

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2848. Eukaryota.
COG0204. LUCA.
HOGENOMiHOG000243517.
InParanoidiQ8GXU8.
KOiK00655.
OMAiMGTGKIM.
OrthoDBiEOG09360IZH.
PhylomeDBiQ8GXU8.

Family and domain databases

InterProiIPR004552. AGP_acyltrans.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00530. AGP_acyltrn. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8GXU8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDVASARSIS SHPSYYGKPI CSSQSSLIRI SRDKVCCFGR ISNGMTSFTT
60 70 80 90 100
SLHAVPSEKF MGETRRTGIQ WSNRSLRHDP YRFLDKKSPR SSQLARDITV
110 120 130 140 150
RADLSGAATP DSSFPEPEIK LSSRLRGIFF CVVAGISATF LIVLMIIGHP
160 170 180 190 200
FVLLFDPYRR KFHHFIAKLW ASISIYPFYK INIEGLENLP SSDTPAVYVS
210 220 230 240 250
NHQSFLDIYT LLSLGKSFKF ISKTGIFVIP IIGWAMSMMG VVPLKRMDPR
260 270 280 290 300
SQVDCLKRCM ELLKKGASVF FFPEGTRSKD GRLGSFKKGA FTVAAKTGVA
310 320 330 340 350
VVPITLMGTG KIMPTGSEGI LNHGNVRVII HKPIHGSKAD VLCNEARSKI

AESMDL
Length:356
Mass (Da):39,395
Last modified:March 1, 2003 - v1
Checksum:i27A9EB5EBE028940
GO
Isoform 2 (identifier: Q8GXU8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-144: Missing.

Note: No experimental confirmation available.
Show »
Length:212
Mass (Da):23,505
Checksum:iD900F72925B88B2F
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0135941 – 144Missing in isoform 2. 1 PublicationAdd BLAST144

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL161577 Genomic DNA. Translation: CAB79776.1.
CP002687 Genomic DNA. Translation: AEE85783.1.
AY136460 mRNA. Translation: AAM97125.1.
BT006253 mRNA. Translation: AAP13361.1.
AK118028 mRNA. Translation: BAC42660.1.
PIRiG85357.
RefSeqiNP_194787.2. NM_119204.4. [Q8GXU8-1]
UniGeneiAt.4579.

Genome annotation databases

EnsemblPlantsiAT4G30580.1; AT4G30580.1; AT4G30580. [Q8GXU8-1]
GeneIDi829181.
GrameneiAT4G30580.1; AT4G30580.1; AT4G30580.
KEGGiath:AT4G30580.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL161577 Genomic DNA. Translation: CAB79776.1.
CP002687 Genomic DNA. Translation: AEE85783.1.
AY136460 mRNA. Translation: AAM97125.1.
BT006253 mRNA. Translation: AAP13361.1.
AK118028 mRNA. Translation: BAC42660.1.
PIRiG85357.
RefSeqiNP_194787.2. NM_119204.4. [Q8GXU8-1]
UniGeneiAt.4579.

3D structure databases

ProteinModelPortaliQ8GXU8.
SMRiQ8GXU8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14468. 1 interactor.
STRINGi3702.AT4G30580.1.

Proteomic databases

PaxDbiQ8GXU8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G30580.1; AT4G30580.1; AT4G30580. [Q8GXU8-1]
GeneIDi829181.
GrameneiAT4G30580.1; AT4G30580.1; AT4G30580.
KEGGiath:AT4G30580.

Organism-specific databases

TAIRiAT4G30580.

Phylogenomic databases

eggNOGiKOG2848. Eukaryota.
COG0204. LUCA.
HOGENOMiHOG000243517.
InParanoidiQ8GXU8.
KOiK00655.
OMAiMGTGKIM.
OrthoDBiEOG09360IZH.
PhylomeDBiQ8GXU8.

Enzyme and pathway databases

UniPathwayiUPA00557; UER00613.
BioCyciMetaCyc:AT4G30580-MONOMER.
BRENDAi2.3.1.51. 399.
ReactomeiR-ATH-1483166. Synthesis of PA.
R-ATH-163765. ChREBP activates metabolic gene expression.
R-ATH-6798695. Neutrophil degranulation.
R-ATH-75109. Triglyceride Biosynthesis.

Miscellaneous databases

PROiQ8GXU8.

Gene expression databases

GenevisibleiQ8GXU8. AT.

Family and domain databases

InterProiIPR004552. AGP_acyltrans.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00530. AGP_acyltrn. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLPAT1_ARATH
AccessioniPrimary (citable) accession number: Q8GXU8
Secondary accession number(s): Q9M0A2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.