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Q8GXT2 (BGL29_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-glucosidase 29

Short name=AtBGLU29
EC=3.2.1.21
Gene names
Name:BGLU29
Ordered Locus Names:At2g44470
ORF Names:F4I1.28
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length590 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Sequence caution

The sequence AAC16093.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8GXT2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Derived from EST data. No experimental confirmation available.
Isoform 2 (identifier: Q8GXT2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     451-451: I → M
     452-590: Missing.
Isoform 3 (identifier: Q8GXT2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     381-397: DDRGKIHSHPEGLRRVL → VCNILIIFIPKILKCFD
     398-590: Missing.
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 590569Beta-glucosidase 29
PRO_0000389591

Regions

Region470 – 4712Substrate binding By similarity

Sites

Active site1971Proton donor By similarity
Active site4131Nucleophile By similarity
Binding site481Substrate By similarity
Binding site1511Substrate By similarity
Binding site1961Substrate By similarity
Binding site3411Substrate By similarity
Binding site4631Substrate By similarity

Amino acid modifications

Glycosylation2551N-linked (GlcNAc...) Potential
Glycosylation3311N-linked (GlcNAc...) Potential
Glycosylation3711N-linked (GlcNAc...) Potential
Glycosylation5221N-linked (GlcNAc...) Potential
Glycosylation5531N-linked (GlcNAc...) Potential
Disulfide bond216 ↔ 224 By similarity

Natural variations

Alternative sequence381 – 39717DDRGK…LRRVL → VCNILIIFIPKILKCFD in isoform 3.
VSP_038460
Alternative sequence398 – 590193Missing in isoform 3.
VSP_038461
Alternative sequence4511I → M in isoform 2.
VSP_038462
Alternative sequence452 – 590139Missing in isoform 2.
VSP_038463

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 24, 2009. Version 2.
Checksum: D0854736ED5FC6E2

FASTA59068,244
        10         20         30         40         50         60 
MNVQIFILLL IISWLTPKIT SLPPESQVLD RSSFPDDFVF GTAISAFQSE GATSEGGKSP 

        70         80         90        100        110        120 
TIWDYFSHTF PERTNMQNAD VAVDFYHRYK DDIKLIEELN VDAFRFSISW ARLIPSGKVK 

       130        140        150        160        170        180 
DGVNKEGVQF YKALIDELIA NGIQPSVTLY HWDHPQALED EYGGFLNPQI IEDFRNFARV 

       190        200        210        220        230        240 
CFENFGDKVK MWTTINEPYV ISVAGYDTGI KAVGRCSKWV NSRCQAGDSA IEPYIVSHHL 

       250        260        270        280        290        300 
LLSHAAAVQE FRNCNKTLQD GKIGIVISPW WLEPYDSTSS ADKEAVERGL PLELEWHLNP 

       310        320        330        340        350        360 
VIYGDYPETM KKHVGNRLPA FTPEQSKMLI NSSDFIGVNY YSIHFTAHLP HIDHTRPRFR 

       370        380        390        400        410        420 
TDHHFEKKLI NRSNHETGPG DDRGKIHSHP EGLRRVLNYI KDKYNNPIVY VKENGIDHYD 

       430        440        450        460        470        480 
DGTKSRETIL KDTFRISYHQ DHLKQVHKAI IEDGCDVRGY YVWSLFDNFE WEHGYNSRFG 

       490        500        510        520        530        540 
MYYVDFKNNL QRYPKDSVNW FKKFLSRPVV RSEETEDEKV CNVSRKEEKI NKALDVSEGF 

       550        560        570        580        590 
KTSVDSIVNL IKNGSRIEEE DDEEERDFCA FKNHNDQLGF FLKLQNSLGF 

« Hide

Isoform 2 [UniParc].

Checksum: BE062A7CDE368D92
Show »

FASTA45151,740
Isoform 3 [UniParc].

Checksum: FAD36442B9C6E55C
Show »

FASTA39745,271

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Functional annotation of a full-length Arabidopsis cDNA collection."
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.
Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Columbia.
[4]"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 1."
Xu Z., Escamilla-Trevino L.L., Zeng L., Lalgondar M., Bevan D.R., Winkel B.S.J., Mohamed A., Cheng C.-L., Shih M.-C., Poulton J.E., Esen A.
Plant Mol. Biol. 55:343-367(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC004521 Genomic DNA. Translation: AAC16093.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC10423.1.
CP002685 Genomic DNA. Translation: AEC10424.1.
CP002685 Genomic DNA. Translation: AEC10425.1.
AK118055 mRNA. Translation: BAC42686.1.
PIRT02402.
RefSeqNP_001078056.1. NM_001084587.1.
NP_001118524.1. NM_001125052.1.
NP_850417.1. NM_180086.1.
UniGeneAt.36755.

3D structure databases

ProteinModelPortalQ8GXT2.
SMRQ8GXT2. Positions 24-507.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbQ8GXT2.
PRIDEQ8GXT2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G44470.3; AT2G44470.3; AT2G44470. [Q8GXT2-1]
GeneID819054.
KEGGath:AT2G44470.

Organism-specific databases

TAIRAT2G44470.

Phylogenomic databases

eggNOGCOG2723.
HOGENOMHOG000088630.
InParanoidQ8GXT2.
KOK01188.
OMAAEMGFKS.
PhylomeDBQ8GXT2.
ProtClustDBCLSN2680239.

Enzyme and pathway databases

BioCycARA:AT2G44470-MONOMER.
ARA:GQT-898-MONOMER.
ARA:GQT-899-MONOMER.

Gene expression databases

GenevestigatorQ8GXT2.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGL29_ARATH
AccessionPrimary (citable) accession number: Q8GXT2
Secondary accession number(s): A8MQN1, O64881
Entry history
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: November 24, 2009
Last modified: April 16, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names