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Protein

Beta-glucosidase 29

Gene

BGLU29

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei48 – 481SubstrateBy similarity
Binding sitei151 – 1511SubstrateBy similarity
Binding sitei196 – 1961SubstrateBy similarity
Active sitei197 – 1971Proton donorBy similarity
Binding sitei341 – 3411SubstrateBy similarity
Active sitei413 – 4131NucleophileBy similarity
Binding sitei463 – 4631SubstrateBy similarity

GO - Molecular functioni

  1. beta-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT2G44470-MONOMER.
ARA:GQT-898-MONOMER.
ARA:GQT-899-MONOMER.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucosidase 29 (EC:3.2.1.21)
Short name:
AtBGLU29
Gene namesi
Name:BGLU29
Ordered Locus Names:At2g44470
ORF Names:F4I1.28
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G44470.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 590569Beta-glucosidase 29PRO_0000389591Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi216 ↔ 224By similarity
Glycosylationi255 – 2551N-linked (GlcNAc...)Sequence Analysis
Glycosylationi331 – 3311N-linked (GlcNAc...)Sequence Analysis
Glycosylationi371 – 3711N-linked (GlcNAc...)Sequence Analysis
Glycosylationi522 – 5221N-linked (GlcNAc...)Sequence Analysis
Glycosylationi553 – 5531N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8GXT2.
PRIDEiQ8GXT2.

Expressioni

Gene expression databases

GenevestigatoriQ8GXT2.

Structurei

3D structure databases

ProteinModelPortaliQ8GXT2.
SMRiQ8GXT2. Positions 29-501.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni470 – 4712Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088630.
InParanoidiQ8GXT2.
KOiK01188.
OMAiMMLARME.
PhylomeDBiQ8GXT2.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8GXT2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNVQIFILLL IISWLTPKIT SLPPESQVLD RSSFPDDFVF GTAISAFQSE
60 70 80 90 100
GATSEGGKSP TIWDYFSHTF PERTNMQNAD VAVDFYHRYK DDIKLIEELN
110 120 130 140 150
VDAFRFSISW ARLIPSGKVK DGVNKEGVQF YKALIDELIA NGIQPSVTLY
160 170 180 190 200
HWDHPQALED EYGGFLNPQI IEDFRNFARV CFENFGDKVK MWTTINEPYV
210 220 230 240 250
ISVAGYDTGI KAVGRCSKWV NSRCQAGDSA IEPYIVSHHL LLSHAAAVQE
260 270 280 290 300
FRNCNKTLQD GKIGIVISPW WLEPYDSTSS ADKEAVERGL PLELEWHLNP
310 320 330 340 350
VIYGDYPETM KKHVGNRLPA FTPEQSKMLI NSSDFIGVNY YSIHFTAHLP
360 370 380 390 400
HIDHTRPRFR TDHHFEKKLI NRSNHETGPG DDRGKIHSHP EGLRRVLNYI
410 420 430 440 450
KDKYNNPIVY VKENGIDHYD DGTKSRETIL KDTFRISYHQ DHLKQVHKAI
460 470 480 490 500
IEDGCDVRGY YVWSLFDNFE WEHGYNSRFG MYYVDFKNNL QRYPKDSVNW
510 520 530 540 550
FKKFLSRPVV RSEETEDEKV CNVSRKEEKI NKALDVSEGF KTSVDSIVNL
560 570 580 590
IKNGSRIEEE DDEEERDFCA FKNHNDQLGF FLKLQNSLGF

Note: Derived from EST data. No experimental confirmation available.

Length:590
Mass (Da):68,244
Last modified:November 23, 2009 - v2
Checksum:iD0854736ED5FC6E2
GO
Isoform 2 (identifier: Q8GXT2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     451-451: I → M
     452-590: Missing.

Show »
Length:451
Mass (Da):51,740
Checksum:iBE062A7CDE368D92
GO
Isoform 3 (identifier: Q8GXT2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     381-397: DDRGKIHSHPEGLRRVL → VCNILIIFIPKILKCFD
     398-590: Missing.

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:397
Mass (Da):45,271
Checksum:iFAD36442B9C6E55C
GO

Sequence cautioni

The sequence AAC16093.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei381 – 39717DDRGK…LRRVL → VCNILIIFIPKILKCFD in isoform 3. CuratedVSP_038460Add
BLAST
Alternative sequencei398 – 590193Missing in isoform 3. CuratedVSP_038461Add
BLAST
Alternative sequencei451 – 4511I → M in isoform 2. 1 PublicationVSP_038462
Alternative sequencei452 – 590139Missing in isoform 2. 1 PublicationVSP_038463Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004521 Genomic DNA. Translation: AAC16093.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC10423.1.
CP002685 Genomic DNA. Translation: AEC10424.1.
CP002685 Genomic DNA. Translation: AEC10425.1.
AK118055 mRNA. Translation: BAC42686.1.
PIRiT02402.
RefSeqiNP_001078056.1. NM_001084587.1. [Q8GXT2-3]
NP_001118524.1. NM_001125052.1. [Q8GXT2-1]
NP_850417.1. NM_180086.1. [Q8GXT2-2]
UniGeneiAt.36755.

Genome annotation databases

EnsemblPlantsiAT2G44470.3; AT2G44470.3; AT2G44470. [Q8GXT2-1]
GeneIDi819054.
KEGGiath:AT2G44470.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004521 Genomic DNA. Translation: AAC16093.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC10423.1.
CP002685 Genomic DNA. Translation: AEC10424.1.
CP002685 Genomic DNA. Translation: AEC10425.1.
AK118055 mRNA. Translation: BAC42686.1.
PIRiT02402.
RefSeqiNP_001078056.1. NM_001084587.1. [Q8GXT2-3]
NP_001118524.1. NM_001125052.1. [Q8GXT2-1]
NP_850417.1. NM_180086.1. [Q8GXT2-2]
UniGeneiAt.36755.

3D structure databases

ProteinModelPortaliQ8GXT2.
SMRiQ8GXT2. Positions 29-501.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbiQ8GXT2.
PRIDEiQ8GXT2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G44470.3; AT2G44470.3; AT2G44470. [Q8GXT2-1]
GeneIDi819054.
KEGGiath:AT2G44470.

Organism-specific databases

TAIRiAT2G44470.

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088630.
InParanoidiQ8GXT2.
KOiK01188.
OMAiMMLARME.
PhylomeDBiQ8GXT2.

Enzyme and pathway databases

BioCyciARA:AT2G44470-MONOMER.
ARA:GQT-898-MONOMER.
ARA:GQT-899-MONOMER.

Gene expression databases

GenevestigatoriQ8GXT2.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  4. Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiBGL29_ARATH
AccessioniPrimary (citable) accession number: Q8GXT2
Secondary accession number(s): A8MQN1, O64881
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2009
Last sequence update: November 23, 2009
Last modified: January 6, 2015
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.