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Protein

Pre-mRNA cleavage factor Im 25 kDa subunit 2

Gene

CFIS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

Metal-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-ATH-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA cleavage factor Im 25 kDa subunit 21 Publication
Gene namesi
Name:CFIS21 Publication
Ordered Locus Names:At4g25550Imported
ORF Names:M7J2.80Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G25550.

Subcellular locationi

  • Nucleus By similarity

  • Note: In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 200200Pre-mRNA cleavage factor Im 25 kDa subunit 2PRO_0000431332Add
BLAST

Proteomic databases

PaxDbiQ8GXS3.
PRIDEiQ8GXS3.

Expressioni

Gene expression databases

GenevisibleiQ8GXS3. AT.

Interactioni

Subunit structurei

Homodimer. Component of the cleavage factor Im (CFIm) complex (By similarity). Forms a complex with cleavage and polyadenylation specificity factor (CPSF) subunits FIPS5, PAPS4 and CPSF30 (PubMed:18479511).By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei33 – 331Interaction with RNABy similarity
Sitei179 – 1791Interaction with RNABy similarity

Protein-protein interaction databases

BioGridi13947. 11 interactions.
IntActiQ8GXS3. 11 interactions.
STRINGi3702.AT4G25550.1.

Structurei

3D structure databases

ProteinModelPortaliQ8GXS3.
SMRiQ8GXS3. Positions 5-195.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 172128Nudix hydrolasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni72 – 743Interaction with RNABy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi79 – 10022Nudix boxPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the Nudix hydrolase family. CPSF5 subfamily.Curated
Contains 1 nudix hydrolase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1689. Eukaryota.
ENOG410XS8Z. LUCA.
HOGENOMiHOG000161320.
InParanoidiQ8GXS3.
KOiK14397.
OMAiGDCLAQW.
OrthoDBiEOG09360MJ2.
PhylomeDBiQ8GXS3.

Family and domain databases

InterProiIPR016706. Cleav_polyA_spec_factor_su5.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view]
PANTHERiPTHR13047. PTHR13047. 1 hit.
PfamiPF13869. NUDIX_2. 1 hit.
[Graphical view]
PIRSFiPIRSF017888. CPSF-25. 1 hit.
SUPFAMiSSF55811. SSF55811. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8GXS3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAMSQVVNTY PLSNYSFGTK EPKLEKDTSV ADRLARMKIN YMKEGMRTSV
60 70 80 90 100
EGILLVQEHN HPHILLLQIG NTFCKLPGGR LKPGENEADG LKRKLTSKLG
110 120 130 140 150
GNSAALVPDW TVGECVATWW RPNFETMMYP YCPPHITKPK ECKRLYIVHL
160 170 180 190 200
SEKEYFAVPK NLKLLAVPLF ELYDNVQRYG PVISTIPQQL SRFHFNMISS
Length:200
Mass (Da):22,830
Last modified:March 1, 2003 - v1
Checksum:i557862865ACB39C8
GO
Isoform 2 (identifier: Q8GXS3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     57-200: Missing.

Show »
Length:56
Mass (Da):6,343
Checksum:i85D29CE09B580D36
GO

Sequence cautioni

The sequence CAA18171 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB81365 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei57 – 200144Missing in isoform 2. VSP_057237Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL022197 Genomic DNA. Translation: CAA18171.1. Sequence problems.
AL161563 Genomic DNA. Translation: CAB81365.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE85076.1.
AK118070 mRNA. Translation: BAC42701.1.
BT005519 mRNA. Translation: AAO63939.1.
AK220576 mRNA. Translation: BAD94845.1.
AK228476 mRNA. Translation: BAF00402.1.
PIRiC85295.
T05792.
RefSeqiNP_194285.2. NM_118687.2. [Q8GXS3-1]
UniGeneiAt.44085.

Genome annotation databases

EnsemblPlantsiAT4G25550.1; AT4G25550.1; AT4G25550. [Q8GXS3-1]
GeneIDi828660.
KEGGiath:AT4G25550.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL022197 Genomic DNA. Translation: CAA18171.1. Sequence problems.
AL161563 Genomic DNA. Translation: CAB81365.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE85076.1.
AK118070 mRNA. Translation: BAC42701.1.
BT005519 mRNA. Translation: AAO63939.1.
AK220576 mRNA. Translation: BAD94845.1.
AK228476 mRNA. Translation: BAF00402.1.
PIRiC85295.
T05792.
RefSeqiNP_194285.2. NM_118687.2. [Q8GXS3-1]
UniGeneiAt.44085.

3D structure databases

ProteinModelPortaliQ8GXS3.
SMRiQ8GXS3. Positions 5-195.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi13947. 11 interactions.
IntActiQ8GXS3. 11 interactions.
STRINGi3702.AT4G25550.1.

Proteomic databases

PaxDbiQ8GXS3.
PRIDEiQ8GXS3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G25550.1; AT4G25550.1; AT4G25550. [Q8GXS3-1]
GeneIDi828660.
KEGGiath:AT4G25550.

Organism-specific databases

TAIRiAT4G25550.

Phylogenomic databases

eggNOGiKOG1689. Eukaryota.
ENOG410XS8Z. LUCA.
HOGENOMiHOG000161320.
InParanoidiQ8GXS3.
KOiK14397.
OMAiGDCLAQW.
OrthoDBiEOG09360MJ2.
PhylomeDBiQ8GXS3.

Enzyme and pathway databases

ReactomeiR-ATH-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

PROiQ8GXS3.

Gene expression databases

GenevisibleiQ8GXS3. AT.

Family and domain databases

InterProiIPR016706. Cleav_polyA_spec_factor_su5.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view]
PANTHERiPTHR13047. PTHR13047. 1 hit.
PfamiPF13869. NUDIX_2. 1 hit.
[Graphical view]
PIRSFiPIRSF017888. CPSF-25. 1 hit.
SUPFAMiSSF55811. SSF55811. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCFIS2_ARATH
AccessioniPrimary (citable) accession number: Q8GXS3
Secondary accession number(s): O65606, Q570Y1, Q9M0K5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 26, 2014
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.