Reviewed, UniProtKB/Swiss-Prot Q8GXQ7 (PUB56_ARATH)

Names and origin

Protein names

Recommended name:
    U-box domain-containing protein 56
    EC=6.3.2.-
Alternative name(s):
    Plant U-box protein 56

Gene names

Name:	PUB56
Ordered Locus Names:	At1g01670
ORF Names:	T1N6.28

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Functions as an E3 ubiquitin ligase By similarity.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Contains 1 U-box domain.
Sequence caution	The sequence AAF78397.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene has been split into 2 genes: At1g01660 and At1g01670.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Domain	Coiled coil
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW protein ubiquitination Inferred from electronic annotation. Source: InterPro
Cellular component	ubiquitin ligase complex Inferred from electronic annotation. Source: InterPro
Molecular function	ubiquitin-protein ligase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	U-box domain-containing protein 56		PRO_0000322191
Regions
	Domain	293 – 364	72	U-box
	Coiled coil	176 – 281	106	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8GXQ7-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 9AC4C736173DD0D4	FASTA	365	41,864
	10 20 30 40 50 60 MTPSSSGLEQ SEIDAIQELE QTSRNDTLLK YHDICIDEGV IEQDVDMSCF SANSVGEWIV 70 80 90 100 110 120 ELIYQNNIKK LIMGATADSH YSEGMVHITP TKADYVIQHA PHCCNIWLVC NGNLIQTREG 130 140 150 160 170 180 RFEHAGSAYS SSSSLHSIDS ALIPYGGAGR AERVTEPHAL SSSEEQSARG IEKMYYEEQR 190 200 210 220 230 240 RRLEIEELKR EKEQRDKMRR VREEALSSSS GVTKILYNEE VMRRREVEAE LNRAKAEIED 250 260 270 280 290 300 MKRVQIELKE QHYADCRLLE KERDEAIKTT EELLRALEKG ESSIPLQWSV SIEPPQCFIC 310 320 330 340 350 360 PISKDIMQNP HVAADGYTYE ADEFRRWLNH GGEKSPMTNL RLENRNLIPN LVLRSAIKDW LQQHP

References

[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed: 11910074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC009273 Genomic DNA. Translation: AAF78397.1. Sequence problems. AK118101 mRNA. Translation: BAC42729.1.
IPI	IPI00528098.
PIR	E86147.
RefSeq	NP_171673.2.
UniGene	At.42637
3D structure databases
HSSP	HSSP built from PDB template 1T1H based on UniProtKB Q8VZ40.
ModBase	Search...
Genome annotation databases
GeneID	839365.
GenomeReviews	Gene locus AT1G01670 in contig CT485782_GR.
KEGG	ath:AT1G01670.
NMPDR	fig|3702.1.peg.278.
Organism-specific databases
TAIR	At1g01670.
Gene expression databases
Genevestigator	Q8GXQ7.
Family and domain databases
InterPro	IPR003613. Ubox_domain. [Graphical view]
Pfam	PF04564. U-box. 1 hit. [Graphical view]
SMART	SM00504. Ubox. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUB56_ARATH

Accession

Primary (citable) accession number: Q8GXQ7
Secondary accession number(s): Q9LQ93

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	March 1, 2003
Last modified:	November 24, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)