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Protein

Protein NRT1/ PTR FAMILY 7.2

Gene

NPF7.2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Low-affinity nitrate transporter. Involved in nitrate removal from xylem sap. Not involved in oligopeptides transport.1 Publication

GO - Molecular functioni

  • nitrate transmembrane transporter activity Source: TAIR
  • symporter activity Source: UniProtKB-KW

GO - Biological processi

  • nitrate assimilation Source: UniProtKB-KW
  • response to cadmium ion Source: TAIR
  • response to nitrate Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Nitrate assimilation, Symport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Protein NRT1/ PTR FAMILY 7.2
Short name:
AtNPF7.2
Alternative name(s):
Nitrate transporter 1.8
Gene namesi
Name:NPF7.2
Synonyms:NRT1.8
Ordered Locus Names:At4g21680
ORF Names:F17L22.140
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G21680.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei32 – 52HelicalSequence analysisAdd BLAST21
Transmembranei78 – 98HelicalSequence analysisAdd BLAST21
Transmembranei105 – 125HelicalSequence analysisAdd BLAST21
Transmembranei147 – 167HelicalSequence analysisAdd BLAST21
Transmembranei187 – 207HelicalSequence analysisAdd BLAST21
Transmembranei217 – 237HelicalSequence analysisAdd BLAST21
Transmembranei343 – 363HelicalSequence analysisAdd BLAST21
Transmembranei377 – 397HelicalSequence analysisAdd BLAST21
Transmembranei423 – 443HelicalSequence analysisAdd BLAST21
Transmembranei464 – 484HelicalSequence analysisAdd BLAST21
Transmembranei504 – 524HelicalSequence analysisAdd BLAST21
Transmembranei548 – 568HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Higher nitrate concentration in xylem sap and increased cadmium sensitivity. A greater proportion of nitrate accumulates in shoots under cadmium stress.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003999811 – 589Protein NRT1/ PTR FAMILY 7.2Add BLAST589

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei102PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8GXN2.
PRIDEiQ8GXN2.

Expressioni

Tissue specificityi

Expressed in xylem parenchyma cells within the vasculature. Expressed in siliques and flowers. Higher expression in shoots than in roots.2 Publications

Inductioni

Up-regulated by cadmium and nitrate.1 Publication

Gene expression databases

GenevisibleiQ8GXN2. AT.

Interactioni

Protein-protein interaction databases

BioGridi13544. 25 interactors.
STRINGi3702.AT4G21680.1.

Structurei

3D structure databases

ProteinModelPortaliQ8GXN2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi455 – 460Poly-Ser6
Compositional biasi585 – 588Poly-Glu4

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1237. Eukaryota.
COG3104. LUCA.
HOGENOMiHOG000237400.
InParanoidiQ8GXN2.
OMAiIFAYRRF.
OrthoDBiEOG093605FC.
PhylomeDBiQ8GXN2.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR020846. MFS_dom.
IPR000109. POT_fam.
[Graphical view]
PANTHERiPTHR11654. PTHR11654. 1 hit.
PfamiPF00854. PTR2. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.

Sequencei

Sequence statusi: Complete.

Q8GXN2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQKVRQFEV CTQDGSVDRH GNPAIRANTG KWLTAILILV NQGLATLAFF
60 70 80 90 100
GVGVNLVLFL TRVMGQDNAE AANNVSKWTG TVYIFSLLGA FLSDSYWGRY
110 120 130 140 150
KTCAIFQASF VAGLMMLSLS TGALLLEPSG CGVEDSPCKP HSTFKTVLFY
160 170 180 190 200
LSVYLIALGY GGYQPNIATF GADQFDAEDS VEGHSKIAFF SYFYLALNLG
210 220 230 240 250
SLFSNTVLGY FEDQGEWPLG FWASAGSAFA GLVLFLIGTP KYRHFTPRES
260 270 280 290 300
PWSRFCQVLV AATRKAKIDV HHEELNLYDS ETQYTGDKKI LHTKGFRFLD
310 320 330 340 350
RAAIVTPDDE AEKVESGSKY DPWRLCSVTQ VEEVKCVLRL LPIWLCTILY
360 370 380 390 400
SVVFTQMASL FVVQGAAMKT NIKNFRIPAS SMSSFDILSV AFFIFAYRRF
410 420 430 440 450
LDPLFARLNK TERNKGLTEL QRMGIGLVIA IMAMISAGIV EIHRLKNKEP
460 470 480 490 500
ESATSISSSS TLSIFWQVPQ YMLIGASEVF MYVGQLEFFN SQAPTGLKSF
510 520 530 540 550
ASALCMASIS LGNYVSSLLV SIVMKISTTD DVHGWIPENL NKGHLERFYF
560 570 580
LLAGLTAADF VVYLICAKWY KYIKSEASFS ESVTEEEEV
Length:589
Mass (Da):65,507
Last modified:November 2, 2010 - v2
Checksum:i0FEF4CF5162067F0
GO

Sequence cautioni

The sequence CAB36812 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB81275 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti389S → N in BAC42767 (PubMed:11910074).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL035527 Genomic DNA. Translation: CAB36812.1. Sequence problems.
AL161555 Genomic DNA. Translation: CAB81275.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84489.1.
AK118142 mRNA. Translation: BAC42767.1.
PIRiT05843.
RefSeqiNP_193899.2. NM_118288.4.
UniGeneiAt.2700.
At.32611.

Genome annotation databases

EnsemblPlantsiAT4G21680.1; AT4G21680.1; AT4G21680.
GeneIDi828255.
GrameneiAT4G21680.1; AT4G21680.1; AT4G21680.
KEGGiath:AT4G21680.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL035527 Genomic DNA. Translation: CAB36812.1. Sequence problems.
AL161555 Genomic DNA. Translation: CAB81275.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84489.1.
AK118142 mRNA. Translation: BAC42767.1.
PIRiT05843.
RefSeqiNP_193899.2. NM_118288.4.
UniGeneiAt.2700.
At.32611.

3D structure databases

ProteinModelPortaliQ8GXN2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi13544. 25 interactors.
STRINGi3702.AT4G21680.1.

Proteomic databases

PaxDbiQ8GXN2.
PRIDEiQ8GXN2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G21680.1; AT4G21680.1; AT4G21680.
GeneIDi828255.
GrameneiAT4G21680.1; AT4G21680.1; AT4G21680.
KEGGiath:AT4G21680.

Organism-specific databases

TAIRiAT4G21680.

Phylogenomic databases

eggNOGiKOG1237. Eukaryota.
COG3104. LUCA.
HOGENOMiHOG000237400.
InParanoidiQ8GXN2.
OMAiIFAYRRF.
OrthoDBiEOG093605FC.
PhylomeDBiQ8GXN2.

Miscellaneous databases

PROiQ8GXN2.

Gene expression databases

GenevisibleiQ8GXN2. AT.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR020846. MFS_dom.
IPR000109. POT_fam.
[Graphical view]
PANTHERiPTHR11654. PTHR11654. 1 hit.
PfamiPF00854. PTR2. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiPTR47_ARATH
AccessioniPrimary (citable) accession number: Q8GXN2
Secondary accession number(s): Q9SVS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: November 2, 2010
Last modified: November 30, 2016
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.