Reviewed, UniProtKB/Swiss-Prot Q8GXL3 (SPL8_ARATH)

Names and origin

Protein names

Recommended name:
    Squamosa promoter-binding-like protein 8

Gene names

Name:	SPL8
Ordered Locus Names:	At1g02065
ORF Names:	T7I23.32

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Trans-acting factor that binds specifically to the consensus nucleotide sequence 5'-TNCGTACAA-3'. Binds specifically to the 5'-GTAC-3' core sequence. Involved in development and floral organogenesis. Required for ovule differentiation, pollen production, filament elongation, seed formation and siliques elongation. Also seems to play a role in the formation of trichomes on sepals. May positively modulate gibberellin (GA) signaling in flower. Ref.4 Ref.5 Ref.6
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subcellular location	Nucleus. Cytoplasm. Note: Mostly located in nucleus. Ref.5
Tissue specificity	Expressed in shoot apical region and early floral tissues. Transcripts levels increase in developing pollen sacs, and decrease in later stage of anther development. Strongly expressed in the placental region of the carpels. Ref.4 Ref.6
Developmental stage	Expressed during plant development. During pollen development, required for crosporogenesis that leads to archesporial formation and for the histogenesis of the microsporangia. During ovules development, involved in the transition to meiosis of the megaspore mother cells. Ref.4 Ref.1
Domain	The SBP-type zinc finger is required for the binding to DNA.
Sequence similarities	Contains 1 SBP-type zinc finger.

Ontologies

Keywords
Biological process	Differentiation Flowering Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Developmental protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW flower development Inferred from electronic annotation. Source: UniProtKB-KW megasporogenesis Ref.4 Inferred from mutant phenotype. Source: TAIR microsporogenesis Ref.4 Inferred from mutant phenotype. Source: TAIR regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q8GXL3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Squamosa promoter-binding-like protein 8		PRO_0000132729
Regions
	Zinc finger	185 – 262	78	SBP-type
	Region	179 – 269	91	Sufficient and necessary for DNA binding
	Motif	245 – 261	17	Bipartite nuclear localization signal Potential
	Compositional bias	281 – 332	52	Ser-rich
Sites
	Metal binding	188	1	Zinc 1 By similarity
	Metal binding	193	1	Zinc 1 By similarity
	Metal binding	210	1	Zinc 1 By similarity
	Metal binding	213	1	Zinc 1 By similarity
	Metal binding	229	1	Zinc 2 By similarity
	Metal binding	232	1	Zinc 2 By similarity
	Metal binding	236	1	Zinc 2 By similarity
	Metal binding	248	1	Zinc 2 By similarity
Experimental info
	Mutagenesis	233	1	S → A: Slight decrease in DNA binding efficiency. Ref.5
	Mutagenesis	247	1	S → A: Slight decrease in DNA binding and nuclear import efficiency. Ref.5
	Mutagenesis	247	1	S → D: Complete loss of DNA binding. Slight increase in nuclear import efficiency. Ref.5
	Mutagenesis	248	1	C → A: Increase in nuclear import efficiency. Ref.5
	Sequence conflict	63	1	H → Q in BAC42797. Ref.3
	Sequence conflict	310	1	Q → R in BAC42797. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 7, 2005. Version 2. Checksum: 0182715A7A35D394	FASTA	333	36,827
	10 20 30 40 50 60 MLDYEWDNPS SIVLSGDERN PDSDPTRSSF SFFDPISHYN NDHRHITISP PLLSSFSNQQ 70 80 90 100 110 120 QQHHLTLYGQ TNSNNQFLHH HHHHHSLYGS TTTTTPYGAS DPIYHPHSSA PPASLFSYDQ 130 140 150 160 170 180 TGPGSGSGSS YNFLIPKTEV DFTSNRIGLN LGGRTYFSAA DDDFVSRLYR RSRPGESGMA 190 200 210 220 230 240 NSLSTPRCQA EGCNADLSHA KHYHRRHKVC EFHSKASTVV AAGLSQRFCQ QCSRFHLLSE 250 260 270 280 290 300 FDNGKRSCRK RLADHNRRRR KCHQSASATQ DTGTGKTTPK SPNDSGVKAS SSPSSNAPPT 310 320 330 ISLECFRQRQ FQTTASSSTS ASSSSNSMFF SSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of the Arabidopsis SBP-box genes." Cardon G.H., Hoehmann S., Klein J., Nettesheim K., Saedler H., Huijser P. Gene 237:91-104(1999) [PubMed: 10524240] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], DEVELOPMENTAL STAGE. Strain: cv. Columbia. Tissue: Flower.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed: 11910074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"SPL8, an SBP-box gene that affects pollen sac development in Arabidopsis." Unte U.S., Sorensen A.-M., Pesaresi P., Gandikota M., Leister D., Saedler H., Huijser P. Plant Cell 15:1009-1019(2003) [PubMed: 12671094] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
[5]	"Functional dissection of the plant-specific SBP-domain: overlap of the DNA-binding and nuclear localization domains." Birkenbihl R.P., Jach G., Saedler H., Huijser P. J. Mol. Biol. 352:585-596(2005) [PubMed: 16095614] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, NUCLEAR LOCALIZATION SIGNAL, REGION, MUTAGENESIS OF SER-233; SER-247 AND CYS-248.
[6]	"SPL8, a local regulator in a subset of gibberellin-mediated developmental processes in Arabidopsis." Zhang Y., Schwarz S., Saedler H., Huijser P. Plant Mol. Biol. 63:429-439(2007) [PubMed: 17093870] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ011641 Genomic DNA. Translation: CAB56593.1. AJ011642 mRNA. Translation: CAB56594.1. U89959 Genomic DNA. No translation available. AK118174 mRNA. Translation: BAC42797.1.
IPI	IPI00535875.
PIR	T52594.
RefSeq	NP_683267.1.
UniGene	At.499
3D structure databases
SMR	Q8GXL3. Positions 188-261.
ModBase	Search...
Genome annotation databases
GeneID	839275.
GenomeReviews	Gene locus AT1G02065 in contig CT485782_GR.
KEGG	ath:AT1G02065.
NMPDR	fig|3702.1.peg.318.
Organism-specific databases
GeneFarm	4769. 472.
TAIR	At1g02065.
Phylogenomic databases
OMA	LGGRTYF
Gene expression databases
Genevestigator	Q8GXL3.
Family and domain databases
InterPro	IPR004333. Transcpt_factor_SBP-box. [Graphical view]
Gene3D	G3DSA:4.10.1100.10. SBP. 1 hit.
Pfam	PF03110. SBP. 1 hit. [Graphical view]
PROSITE	PS51141. ZF_SBP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPL8_ARATH

Accession

Primary (citable) accession number: Q8GXL3
Secondary accession number(s): Q9S849

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	November 3, 2009
This is version 41 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)