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Protein

Glutamate receptor 3.4

Gene

GLR3.4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Glutamate-gated receptor that probably acts as non-selective cation channel, at least in hypocotyls. Can be triggered by Ala, Asn, Gly, Ser and, to a lower extent, Cys and Glu. May be involved in light-signal transduction and calcium homeostasis via the regulation of calcium influx into cells. Plays an important role in the calcium-based fast transmission of environmental stress.3 Publications

GO - Molecular functioni

  • calcium channel activity Source: UniProtKB
  • glutamate receptor activity Source: UniProtKB
  • ionotropic glutamate receptor activity Source: InterPro
  • ligand-gated ion channel activity Source: TAIR

GO - Biological processi

  • calcium ion transmembrane transport Source: GOC
  • calcium ion transport Source: UniProtKB
  • calcium-mediated signaling Source: UniProtKB
  • cellular response to acetate Source: UniProtKB
  • cellular response to amino acid stimulus Source: UniProtKB
  • cellular response to cold Source: UniProtKB
  • cellular response to mechanical stimulus Source: UniProtKB
  • ion transmembrane transport Source: GOC
  • response to wounding Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Stress response, Transport

Protein family/group databases

TCDBi1.A.10.1.10. the glutamate-gated ion channel (gic) family of neurotransmitter receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate receptor 3.4
Short name:
AtGLR4
Alternative name(s):
Ligand-gated ion channel 3.4
Gene namesi
Name:GLR3.4
Synonyms:GLR4, GLUR3
Ordered Locus Names:At1g05200
ORF Names:YUP8H12.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G05200.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini36 – 613578ExtracellularSequence analysisAdd
BLAST
Transmembranei614 – 63421HelicalSequence analysisAdd
BLAST
Topological domaini635 – 6439CytoplasmicSequence analysis
Transmembranei644 – 66421HelicalSequence analysisAdd
BLAST
Topological domaini665 – 67511CytoplasmicSequence analysisAdd
BLAST
Transmembranei676 – 69621HelicalSequence analysisAdd
BLAST
Topological domaini697 – 857161ExtracellularSequence analysisAdd
BLAST
Transmembranei858 – 87821HelicalSequence analysisAdd
BLAST
Topological domaini879 – 95981CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • chloroplast Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB
  • plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Chloroplast, Membrane, Plastid

Pathology & Biotechi

Disruption phenotypei

Impaired glutamate-triggered (and Ala, Asn, Cys, Gly, Ser-triggered) membrane depolarization and calcium rise. Slight reduction of photosynthetic yield of Photosystem II.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535Sequence analysisAdd
BLAST
Chaini36 – 959924Glutamate receptor 3.4PRO_0000011608Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi38 – 381N-linked (GlcNAc...)Sequence analysis
Glycosylationi42 – 421N-linked (GlcNAc...)Sequence analysis
Glycosylationi108 – 1081N-linked (GlcNAc...)Sequence analysis
Glycosylationi365 – 3651N-linked (GlcNAc...)Sequence analysis
Glycosylationi378 – 3781N-linked (GlcNAc...)Sequence analysis
Glycosylationi404 – 4041N-linked (GlcNAc...)Sequence analysis
Glycosylationi443 – 4431N-linked (GlcNAc...)Sequence analysis
Glycosylationi461 – 4611N-linked (GlcNAc...)Sequence analysis
Glycosylationi576 – 5761N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8GXJ4.
PRIDEiQ8GXJ4.

PTM databases

iPTMnetiQ8GXJ4.

Expressioni

Tissue specificityi

Expressed in seedlings, cotyledons, roots (e.g. root hairs, epidermis and cortex cells), stems, leaves (e.g. vascular bundles and hydathodes), and siliques.2 Publications

Inductioni

The induction by glutamate, gamma-amino butiric acid (GABA), malate, aspartate, acetate, wounding, touch, and cold stress stimuli is abscisic acid (ABA)-independent, but calcium-dependent. Cold-mediated induction is rapid but transient.1 Publication

Gene expression databases

ExpressionAtlasiQ8GXJ4. baseline and differential.
GenevisibleiQ8GXJ4. AT.

Interactioni

Subunit structurei

May form heteromers.By similarity

Protein-protein interaction databases

BioGridi24503. 22 interactions.
STRINGi3702.AT1G05200.1.

Structurei

3D structure databases

ProteinModelPortaliQ8GXJ4.
SMRiQ8GXJ4. Positions 64-876.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410ISQZ. Eukaryota.
ENOG410ZZ8P. LUCA.
HOGENOMiHOG000239558.
InParanoidiQ8GXJ4.
KOiK05387.
OMAiECSMQIS.
PhylomeDBiQ8GXJ4.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR001320. Iontro_rcpt.
IPR017103. Iontropic_Glu_rcpt_pln.
IPR028082. Peripla_BP_I.
IPR001638. Solute-binding_3/MltF_N.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF00497. SBP_bac_3. 1 hit.
[Graphical view]
PIRSFiPIRSF037090. Iontro_Glu-like_rcpt_pln. 1 hit.
SMARTiSM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8GXJ4-1) [UniParc]FASTAAdd to basket

Also known as: GLR3.4a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGFLVMIREV SMAKAIRVVL LCVSVLWVVP KECACRSNFS RNSSSSSSSS
60 70 80 90 100
LRPLRQRPSS VNVGALFTYD SFIGRAAKPA VKAAMDDVNA DQSVLKGIKL
110 120 130 140 150
NIIFQDSNCS GFIGTMGALQ LMENKVVAAI GPQSSGIAHM ISYVANELHV
160 170 180 190 200
PLLSFGATDP TLSSLQFPYF LRTTQNDYFQ MHAIADFLSY SGWRQVIAIF
210 220 230 240 250
VDDECGRNGI SVLGDVLAKK RSRISYKAAI TPGADSSSIR DLLVSVNLME
260 270 280 290 300
SRVFVVHVNP DSGLNVFSVA KSLGMMASGY VWIATDWLPT AMDSMEHVDS
310 320 330 340 350
DTMDLLQGVV AFRHYTIESS VKRQFMARWK NLRPNDGFNS YAMYAYDSVW
360 370 380 390 400
LVARALDVFF RENNNITFSN DPNLHKTNGS TIQLSALSVF NEGEKFMKII
410 420 430 440 450
LGMNHTGVTG PIQFDSDRNR VNPAYEVLNL EGTAPRTVGY WSNHSGLSVV
460 470 480 490 500
HPETLYSRPP NTSTANQRLK GIIYPGEVTK PPRGWVFPNN GKPLRIGVPN
510 520 530 540 550
RVSYTDYVSK DKNPPGVRGY CIDVFEAAIE LLPYPVPRTY ILYGDGKRNP
560 570 580 590 600
SYDNLVNEVV ADNFDVAVGD ITIVTNRTRY VDFTQPFIES GLVVVAPVKE
610 620 630 640 650
AKSSPWSFLK PFTIEMWAVT GGFFLFVGAM VWILEHRFNQ EFRGPPRRQL
660 670 680 690 700
ITIFWFSFST MFFSHRENTV SSLGRFVLII WLFVVLIINS SYTASLTSIL
710 720 730 740 750
TIRQLTSRIE GIDSLVTSNE PIGVQDGTFA RNYLINELNI LPSRIVPLKD
760 770 780 790 800
EEQYLSALQR GPNAGGVAAI VDELPYIEVL LTNSNCKFRT VGQEFTRTGW
810 820 830 840 850
GFAFQRDSPL AVDMSTAILQ LSEEGELEKI HRKWLNYKHE CSMQISNSED
860 870 880 890 900
SQLSLKSFWG LFLICGITCF MALTVFFWRV FWQYQRLLPE SADEERAGEV
910 920 930 940 950
SEPSRSGRGS RAPSFKELIK VVDKREAEIK EILKQKSSKK LKSTQSAAGT

SQSQHGEIT
Length:959
Mass (Da):107,207
Last modified:January 16, 2004 - v2
Checksum:i0709B834A76FAFC0
GO
Isoform 2 (identifier: Q8GXJ4-2) [UniParc]FASTAAdd to basket

Also known as: GLR3.4b

The sequence of this isoform differs from the canonical sequence as follows:
     656-669: FSFSTMFFSHRENT → LVSQFLTLEPEFTF
     670-959: Missing.

Note: May be due to an intron retention. No experimental confirmation available.
Show »
Length:669
Mass (Da):74,551
Checksum:i623551179F90EBFD
GO

Sequence cautioni

The sequence AAB71458.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAD47833.1 differs from that shown. Reason: Erroneous termination at position 953. Translated as Ser.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti57 – 571R → G in AAD47833 (PubMed:15864638).Curated
Sequence conflicti152 – 1521L → P in AAD47833 (PubMed:15864638).Curated
Sequence conflicti444 – 4441H → Q in AAD47833 (PubMed:15864638).Curated
Sequence conflicti450 – 4501V → A in BAC42828 (PubMed:11910074).Curated
Sequence conflicti733 – 7331Y → N in AAD47833 (PubMed:15864638).Curated
Sequence conflicti897 – 8971A → P in AAD47833 (PubMed:15864638).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei656 – 66914FSFST…HRENT → LVSQFLTLEPEFTF in isoform 2. 1 PublicationVSP_009219Add
BLAST
Alternative sequencei670 – 959290Missing in isoform 2. 1 PublicationVSP_009220Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY072070 mRNA. Translation: AAL61999.1.
AC000098 Genomic DNA. Translation: AAB71458.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE27803.1.
CP002684 Genomic DNA. Translation: AEE27804.1.
AK118206 mRNA. Translation: BAC42828.1.
AF167355 mRNA. Translation: AAD47833.1. Sequence problems.
PIRiD86186.
T51135.
RefSeqiNP_001030971.1. NM_001035894.1. [Q8GXJ4-1]
NP_172012.2. NM_100398.2. [Q8GXJ4-1]
UniGeneiAt.10412.
At.66937.

Genome annotation databases

EnsemblPlantsiAT1G05200.1; AT1G05200.1; AT1G05200. [Q8GXJ4-1]
AT1G05200.2; AT1G05200.2; AT1G05200. [Q8GXJ4-1]
GeneIDi839268.
KEGGiath:AT1G05200.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY072070 mRNA. Translation: AAL61999.1.
AC000098 Genomic DNA. Translation: AAB71458.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE27803.1.
CP002684 Genomic DNA. Translation: AEE27804.1.
AK118206 mRNA. Translation: BAC42828.1.
AF167355 mRNA. Translation: AAD47833.1. Sequence problems.
PIRiD86186.
T51135.
RefSeqiNP_001030971.1. NM_001035894.1. [Q8GXJ4-1]
NP_172012.2. NM_100398.2. [Q8GXJ4-1]
UniGeneiAt.10412.
At.66937.

3D structure databases

ProteinModelPortaliQ8GXJ4.
SMRiQ8GXJ4. Positions 64-876.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi24503. 22 interactions.
STRINGi3702.AT1G05200.1.

Protein family/group databases

TCDBi1.A.10.1.10. the glutamate-gated ion channel (gic) family of neurotransmitter receptors.

PTM databases

iPTMnetiQ8GXJ4.

Proteomic databases

PaxDbiQ8GXJ4.
PRIDEiQ8GXJ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G05200.1; AT1G05200.1; AT1G05200. [Q8GXJ4-1]
AT1G05200.2; AT1G05200.2; AT1G05200. [Q8GXJ4-1]
GeneIDi839268.
KEGGiath:AT1G05200.

Organism-specific databases

TAIRiAT1G05200.

Phylogenomic databases

eggNOGiENOG410ISQZ. Eukaryota.
ENOG410ZZ8P. LUCA.
HOGENOMiHOG000239558.
InParanoidiQ8GXJ4.
KOiK05387.
OMAiECSMQIS.
PhylomeDBiQ8GXJ4.

Miscellaneous databases

PROiQ8GXJ4.

Gene expression databases

ExpressionAtlasiQ8GXJ4. baseline and differential.
GenevisibleiQ8GXJ4. AT.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR001320. Iontro_rcpt.
IPR017103. Iontropic_Glu_rcpt_pln.
IPR028082. Peripla_BP_I.
IPR001638. Solute-binding_3/MltF_N.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF00497. SBP_bac_3. 1 hit.
[Graphical view]
PIRSFiPIRSF037090. Iontro_Glu-like_rcpt_pln. 1 hit.
SMARTiSM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Phylogenetic and expression analysis of the glutamate-receptor-like gene family in Arabidopsis thaliana."
    Chiu J.C., Brenner E.D., DeSalle R., Nitabach M.N., Holmes T.C., Coruzzi G.M.
    Mol. Biol. Evol. 19:1066-1082(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  5. "AtGLR3.4, a glutamate receptor channel-like gene is sensitive to touch and cold."
    Meyerhoff O., Mueller K., Roelfsema M.R.G., Latz A., Lacombe B., Hedrich R., Dietrich P., Becker D.
    Planta 222:418-427(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 12-959 (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INDUCTION BY ABIOTIC STRESSES, SUBCELLULAR LOCATION.
    Strain: cv. Columbia.
  6. Cited for: GENE FAMILY, NOMENCLATURE.
  7. "Glutamate receptor subtypes evidenced by differences in desensitization and dependence on the GLR3.3 and GLR3.4 genes."
    Stephens N.R., Qi Z., Spalding E.P.
    Plant Physiol. 146:529-538(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  8. "Dual localization of plant glutamate receptor AtGLR3.4 to plastids and plasmamembrane."
    Teardo E., Formentin E., Segalla A., Giacometti G.M., Marin O., Zanetti M., Lo Schiavo F., Zoratti M., Szabo I.
    Biochim. Biophys. Acta 1807:359-367(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiGLR34_ARATH
AccessioniPrimary (citable) accession number: Q8GXJ4
Secondary accession number(s): O23048, Q8LGM9, Q9SWD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 16, 2004
Last modified: June 8, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.