Q8GXG6 (B3GTH_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable beta-1,3-galactosyltransferase 17
EC=2.4.1.-

Gene names

Name:	B3GALT17
Ordered Locus Names:	At1g27120
ORF Names:	T7N9.18

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	673 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal glycosyl residue By similarity.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Probable.
Tissue specificity	Expressed in stems, cauline leaves and siliques. Ref.4
Sequence similarities	Belongs to the glycosyltransferase 31 family. Contains 1 galectin domain.
Sequence caution	The sequence AAF79857.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	galactosyltransferase activity Inferred from electronic annotation. Source: InterPro sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 673	673	Probable beta-1,3-galactosyltransferase 17		PRO_0000359427
Regions
	Transmembrane	20 – 40	21	Helical; Signal-anchor for type II membrane protein; Potential
	Domain	185 – 394	210	Galectin
Amino acid modifications
	Glycosylation	8	1	N-linked (GlcNAc...) Potential
	Glycosylation	116	1	N-linked (GlcNAc...) Potential
	Glycosylation	182	1	N-linked (GlcNAc...) Potential
	Glycosylation	621	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	460	1	V → A in BAC42872. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8GXG6 [UniParc]. Last modified January 20, 2009. Version 2. Checksum: 758489AD9DDC85E6	FASTA	673	77,037
	10 20 30 40 50 60 MKKSKLDNSS SQIRFGLVQF LLVVLLFYFL CMSFEIPFIF RTGSGSGSDD VSSSSFADAL 70 80 90 100 110 120 PRPMVVGGGS REANWVVGEE EEADPHRHFK DPGRVQLRLP ERKMREFKSV SEIFVNESFF 130 140 150 160 170 180 DNGGFSDEFS IFHKTAKHAI SMGRKMWDGL DSGLIKPDKA PVKTRIEKCP DMVSVSESEF 190 200 210 220 230 240 VNRSRILVLP CGLTLGSHIT VVATPHWAHV EKDGDKTAMV SQFMMELQGL KAVDGEDPPR 250 260 270 280 290 300 ILHFNPRIKG DWSGRPVIEQ NTCYRMQWGS GLRCDGRESS DDEEYVDGEV KCERWKRDDD 310 320 330 340 350 360 DGGNNGDDFD ESKKTWWLNR LMGRRKKMIT HDWDYPFAEG KLFVLTLRAG MEGYHISVNG 370 380 390 400 410 420 RHITSFPYRT GFVLEDATGL AVKGNIDVHS VYAASLPSTN PSFAPQKHLE MQRIWKAPSL 430 440 450 460 470 480 PQKPVELFIG ILSAGNHFAE RMAVRKSWMQ QKLVRSSKVV ARFFVALHAR KEVNVDLKKE 490 500 510 520 530 540 AEYFGDIVIV PYMDHYDLVV LKTVAICEYG VNTVAAKYVM KCDDDTFVRV DAVIQEAEKV 550 560 570 580 590 600 KGRESLYIGN INFNHKPLRT GKWAVTFEEW PEEYYPPYAN GPGYILSYDV AKFIVDDFEQ 610 620 630 640 650 660 KRLRLFKMED VSMGMWVEKF NETRPVAVVH SLKFCQFGCI EDYFTAHYQS PRQMICMWDK 670 LQRLGKPQCC NMR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed: 11910074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"A unique beta-1,3-galactosyltransferase is indispensable for the biosynthesis of N-glycans containing Lewis a structures in Arabidopsis thaliana." Strasser R., Bondili J.S., Vavra U., Schoberer J., Svoboda B., Gloessl J., Leonard R., Stadlmann J., Altmann F., Steinkellner H., Mach L. Plant Cell 19:2278-2292(2007) [PubMed: 17630273] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Identification of a novel group of putative Arabidopsis thaliana beta-(1,3)-galactosyltransferases." Qu Y., Egelund J., Gilson P.R., Houghton F., Gleeson P.A., Schultz C.J., Bacic A. Plant Mol. Biol. 68:43-59(2008) [PubMed: 18548197] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC000348 Genomic DNA. Translation: AAF79857.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE30783.1. AK118254 mRNA. Translation: BAC42872.1.
IPI	IPI00531027.
PIR	G86397.
RefSeq	NP_174032.2. NM_102474.3.
UniGene	At.16267.
3D structure databases
ProteinModelPortal	Q8GXG6.
ModBase	Search...
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31.
Proteomic databases
PRIDE	Q8GXG6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G27120.1; AT1G27120.1; AT1G27120.
GeneID	839601.
GenomeReviews	Gene locus AT1G27120 in contig CT485782_GR.
KEGG	ath:AT1G27120.
Organism-specific databases
TAIR	At1g27120.
Phylogenomic databases
eggNOG	KOG2287.
GeneTree	EPGT00050000005432.
HOGENOM	HBG318248.
InParanoid	Q8GXG6.
OMA	FVALHAR.
PhylomeDB	Q8GXG6.
ProtClustDB	CLSN2918262.
Gene expression databases
Genevestigator	Q8GXG6.
Family and domain databases
InterPro	IPR008985. ConA-like_lec_gl. IPR013320. ConA-like_subgrp. IPR001079. Galectin_CRD. IPR002659. Glyco_trans_31. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 2 hits.
PANTHER	PTHR11214. Glyco_trans_31. 1 hit.
Pfam	PF00337. Gal-bind_lectin. 1 hit. PF01762. Galactosyl_T. 1 hit. [Graphical view]
SMART	SM00908. Gal-bind_lectin. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS51304. GALECTIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B3GTH_ARATH

Accession

Primary (citable) accession number: Q8GXG6
Secondary accession number(s): Q9LFX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	January 20, 2009
Last modified:	December 14, 2011
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program