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Protein

Probable isoaspartyl peptidase/L-asparaginase 2

Gene

At3g16150

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Acts in asparagine catabolism and also in the final steps of protein degradation via hydrolysis of a range of isoaspartyl dipeptides.By similarity

Catalytic activityi

Cleavage of a beta-linked Asp residue from the N-terminus of a polypeptide.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei195 – 1951NucleophileBy similarity

GO - Molecular functioni

  • asparaginase activity Source: TAIR
  • beta-aspartyl-peptidase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease

Enzyme and pathway databases

BioCyciARA:AT3G16150-MONOMER.
BRENDAi3.5.1.1. 399.
ReactomeiR-ATH-71182. Phenylalanine and tyrosine catabolism.
SABIO-RKQ8GXG1.

Protein family/group databases

MEROPSiT02.A02.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable isoaspartyl peptidase/L-asparaginase 2 (EC:3.4.19.5)
Alternative name(s):
L-asparagine amidohydrolase 2
Cleaved into the following 2 chains:
Gene namesi
Ordered Locus Names:At3g16150
ORF Names:MSL1.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G16150.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 194194Isoaspartyl peptidase/L-asparaginase 2 subunit alphaPRO_0000045444Add
BLAST
Chaini195 – 325131Isoaspartyl peptidase/L-asparaginase 2 subunit betaPRO_0000045445Add
BLAST

Post-translational modificationi

Cleaved into an alpha and beta chain by autocatalysis; this activates the enzyme. The N-terminal residue of the beta subunit is responsible for the nucleophile hydrolase activity (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei194 – 1952Cleavage; by autolysisBy similarity

Keywords - PTMi

Autocatalytic cleavage

Proteomic databases

PaxDbiQ8GXG1.
PRIDEiQ8GXG1.

Expressioni

Gene expression databases

ExpressionAtlasiQ8GXG1. baseline and differential.
GenevisibleiQ8GXG1. AT.

Interactioni

Subunit structurei

Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers.By similarity

Protein-protein interaction databases

STRINGi3702.AT3G16150.1.

Structurei

3D structure databases

ProteinModelPortaliQ8GXG1.
SMRiQ8GXG1. Positions 2-158, 195-325.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni223 – 2264Substrate bindingBy similarity
Regioni245 – 2484Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the Ntn-hydrolase family.Curated

Phylogenomic databases

eggNOGiKOG1592. Eukaryota.
COG1446. LUCA.
HOGENOMiHOG000174613.
InParanoidiQ8GXG1.
KOiK13051.
OMAiVMEYKEL.
PhylomeDBiQ8GXG1.

Family and domain databases

InterProiIPR029055. Ntn_hydrolases_N.
IPR000246. Peptidase_T2.
[Graphical view]
PANTHERiPTHR10188. PTHR10188. 1 hit.
PfamiPF01112. Asparaginase_2. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8GXG1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGGWAIAVHG GAGIDPNLPA ERQEEAKQLL TRCLNLGIIA LRSNVSAIDV
60 70 80 90 100
VELVIRELET DPLFNSGRGS ALTEKGTVEM EASIMDGTKR RCGAVSGITT
110 120 130 140 150
VKNPISLARL VMDKSPHSYL AFSGAEDFAR KQGVEIVDNE YFVTDDNVGM
160 170 180 190 200
LKLAKEANSI LFDYRIPPMG CAGAAATDSP IQMNGLPISI YAPETVGCVV
210 220 230 240 250
VDGKGHCAAG TSTGGLMNKM MGRIGDSPLI GAGTYASEFC GVSCTGEGEA
260 270 280 290 300
IIRATLARDV SAVMEYKGLN LQEAVDYVIK HRLDEGFAGL IAVSNKGEVV
310 320
CGFNSNGMFR GCATEDGFME VAIWE
Length:325
Mass (Da):34,341
Last modified:January 10, 2006 - v2
Checksum:i91641FA109B50519
GO
Isoform 2 (identifier: Q8GXG1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-149: Missing.

Note: May be due to intron retention. No experimental confirmation available.
Show »
Length:176
Mass (Da):18,384
Checksum:i038D8E78BBDCD299
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 149149Missing in isoform 2. 1 PublicationVSP_016936Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012247 Genomic DNA. Translation: BAB02681.1.
CP002686 Genomic DNA. Translation: AEE75777.1.
AK118259 mRNA. Translation: BAC42877.1.
AY086130 mRNA. Translation: AAM63335.1.
RefSeqiNP_566536.1. NM_112485.3. [Q8GXG1-1]
UniGeneiAt.38938.

Genome annotation databases

EnsemblPlantsiAT3G16150.1; AT3G16150.1; AT3G16150. [Q8GXG1-1]
GeneIDi820860.
KEGGiath:AT3G16150.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012247 Genomic DNA. Translation: BAB02681.1.
CP002686 Genomic DNA. Translation: AEE75777.1.
AK118259 mRNA. Translation: BAC42877.1.
AY086130 mRNA. Translation: AAM63335.1.
RefSeqiNP_566536.1. NM_112485.3. [Q8GXG1-1]
UniGeneiAt.38938.

3D structure databases

ProteinModelPortaliQ8GXG1.
SMRiQ8GXG1. Positions 2-158, 195-325.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G16150.1.

Protein family/group databases

MEROPSiT02.A02.

Proteomic databases

PaxDbiQ8GXG1.
PRIDEiQ8GXG1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G16150.1; AT3G16150.1; AT3G16150. [Q8GXG1-1]
GeneIDi820860.
KEGGiath:AT3G16150.

Organism-specific databases

TAIRiAT3G16150.

Phylogenomic databases

eggNOGiKOG1592. Eukaryota.
COG1446. LUCA.
HOGENOMiHOG000174613.
InParanoidiQ8GXG1.
KOiK13051.
OMAiVMEYKEL.
PhylomeDBiQ8GXG1.

Enzyme and pathway databases

BioCyciARA:AT3G16150-MONOMER.
BRENDAi3.5.1.1. 399.
ReactomeiR-ATH-71182. Phenylalanine and tyrosine catabolism.
SABIO-RKQ8GXG1.

Miscellaneous databases

PROiQ8GXG1.

Gene expression databases

ExpressionAtlasiQ8GXG1. baseline and differential.
GenevisibleiQ8GXG1. AT.

Family and domain databases

InterProiIPR029055. Ntn_hydrolases_N.
IPR000246. Peptidase_T2.
[Graphical view]
PANTHERiPTHR10188. PTHR10188. 1 hit.
PfamiPF01112. Asparaginase_2. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).

Entry informationi

Entry nameiASPGB_ARATH
AccessioniPrimary (citable) accession number: Q8GXG1
Secondary accession number(s): Q9LW72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 10, 2006
Last modified: February 17, 2016
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.