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Protein

Potassium channel AKT6

Gene

AKT6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Highly selective inward-rectifying potassium channel that could mediate potassium uptake in the pollen membrane. Plays an important role in pollen tube development. Assuming opened or closed conformations in response to the voltage difference across the membrane, the channel is activated by hyperpolarization. May interact with the cytoskeleton or with regulatory proteins.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi398 – 519122cNMPAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

BioCyciARA:GQT-2555-MONOMER.
ReactomeiR-ATH-1296072. Voltage gated Potassium channels.

Protein family/group databases

TCDBi1.A.1.4.8. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel AKT6
Alternative name(s):
Potassium channel SPIK
Shaker pollen inward rectifier K(+) channel
Gene namesi
Name:AKT6
Synonyms:SPIK
Ordered Locus Names:At2g25600
ORF Names:F3N11.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G25600.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8484CytoplasmicSequence analysisAdd
BLAST
Transmembranei85 – 10521Helical; Name=Segment S1Sequence analysisAdd
BLAST
Topological domaini106 – 1138ExtracellularSequence analysis
Transmembranei114 – 13421Helical; Name=Segment S2Sequence analysisAdd
BLAST
Topological domaini135 – 15521CytoplasmicSequence analysisAdd
BLAST
Transmembranei156 – 17621Helical; Name=Segment S3Sequence analysisAdd
BLAST
Topological domaini177 – 1848ExtracellularSequence analysis
Transmembranei185 – 20521Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd
BLAST
Topological domaini206 – 21914CytoplasmicSequence analysisAdd
BLAST
Transmembranei220 – 24021Helical; Name=Segment S5Sequence analysisAdd
BLAST
Topological domaini241 – 26727ExtracellularSequence analysisAdd
BLAST
Intramembranei268 – 28720Pore-forming; Name=Segment H5Sequence analysisAdd
BLAST
Topological domaini288 – 2914ExtracellularSequence analysis
Transmembranei292 – 31221Helical; Name=Segment S6Sequence analysisAdd
BLAST
Topological domaini313 – 888576CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 888888Potassium channel AKT6PRO_0000054124Add
BLAST

Proteomic databases

PaxDbiQ8GXE6.
PRIDEiQ8GXE6.

Expressioni

Tissue specificityi

Predominantly expressed in flowers; especially in pollen.2 Publications

Gene expression databases

GenevisibleiQ8GXE6. AT.

Interactioni

Subunit structurei

The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits.By similarity

Protein-protein interaction databases

BioGridi2451. 7 interactions.
STRINGi3702.AT2G25600.1.

Structurei

3D structure databases

ProteinModelPortaliQ8GXE6.
SMRiQ8GXE6. Positions 325-874.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati543 – 57230ANK 1Add
BLAST
Repeati576 – 60530ANK 2Add
BLAST
Repeati609 – 63830ANK 3Add
BLAST
Repeati640 – 66930ANK 4Add
BLAST
Repeati673 – 70230ANK 5Add
BLAST
Domaini822 – 88867KHAPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi15 – 2410Poly-Gly

Domaini

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids. The pore-forming region H5 is enclosed by the transmembrane segments S5 and S6 in the Shaker-type (1P/6TM) and contains the GYGD signature motif which seems to be involved in potassium selectivity.
The KHA domain (rich in hydrophobic and acidic residues) present in the C-terminal part is likely to be important for tetramerization.

Sequence similaritiesi

Contains 5 ANK repeats.PROSITE-ProRule annotation
Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
Contains 1 KHA domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0498. Eukaryota.
ENOG410XPSE. LUCA.
HOGENOMiHOG000238230.
InParanoidiQ8GXE6.
OMAiHESIQGY.
PhylomeDBiQ8GXE6.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR021789. KHA_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF11834. KHA. 1 hit.
[Graphical view]
PRINTSiPR01463. EAGCHANLFMLY.
SMARTiSM00248. ANK. 4 hits.
SM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS50042. CNMP_BINDING_3. 1 hit.
PS51490. KHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8GXE6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKKKVWFWG VKDDGEGGGG RGGGRTKDAE DDVADHLSRD GTMSQYSLSK
60 70 80 90 100
GLLPSLGANN RSSRDVILPR FIVSPFDPRY RAWETFLVFL VLYTAWASPF
110 120 130 140 150
EFGFLQKPRP PLSILDNIVN GFFAVDIVLT FFVAFLDKVT YLLVDDPKRI
160 170 180 190 200
AWRYASTWLI FDVVSTFPYE IFGSLLHESI QGYGIFSMLR LWRLRRVSNC
210 220 230 240 250
FARLEKDRKY SYFWVRCSKL LLVTLFVIHC GACFLYSIAA HYPDPSKTFM
260 270 280 290 300
ALTDENWKES PIAVRYNTAM YWSITTFSTT GYGDIHGVNS REMTFILFYM
310 320 330 340 350
VFNLGLSAYI IGNMTNLVVH VTGRTRKFRD TIQAASGFGQ RNNLPVRLQD
360 370 380 390 400
QMVAHLCLRY RTDSEGLQQQ EIIDSLPKAI RSSISHYLFY EVVDKIYLFH
410 420 430 440 450
GISNDLLFQL VTEMKAEYFP PKEDVILQNE APTDFYILVT GAVDIIARVN
460 470 480 490 500
GVEQVVSEAQ RGHVFGEVGV LCYRPQLFTV RTKRLSQLLR LNRTVLLNLV
510 520 530 540 550
QANVGDGAII MNNLLQHLKD SEDPVMKGVL ADTEHMLAQG KMDLPLSLCF
560 570 580 590 600
AAARGDDLLL HQLLRRGSSP NEMDKDGRTA LHIAASKGSH YCVVLLLEHG
610 620 630 640 650
ADPNIRDSEG NVPLWEAIIG RHREIAKLLA ENGAKLSLDS VSYFSGLAVE
660 670 680 690 700
KNCLDALKDI IKYGGDVTLP DGNGTTALHR AVSEGHLEIV KFLLDQGADL
710 720 730 740 750
DWPDSYGWTP RGLADHQGNE EIKTLFHNHR PVEKKPKPIP GIPQSPVTGK
760 770 780 790 800
PLMKYSSEPT MHSGELVLDG GQVVVSQKRK LNNFRNSLFG IISAANSADD
810 820 830 840 850
GGEVPRSPAV PGGGGSMIYP ERVTISSPEN GETGGKVVLL PNSMEELLKI
860 870 880
GENKMGFVPT KVLTREGAEI DDITLIRDGD FLLLSRDP
Length:888
Mass (Da):99,215
Last modified:March 1, 2004 - v2
Checksum:i60479CE59404505E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ309323 Genomic DNA. Translation: CAC85283.1.
AC006053 Genomic DNA. Translation: AAD31377.1.
CP002685 Genomic DNA. Translation: AEC07722.1.
AK118279 mRNA. Translation: BAC42897.1.
PIRiD84650.
RefSeqiNP_180131.3. NM_128118.4.
UniGeneiAt.39001.

Genome annotation databases

EnsemblPlantsiAT2G25600.1; AT2G25600.1; AT2G25600.
GeneIDi817099.
GrameneiAT2G25600.1; AT2G25600.1; AT2G25600.
KEGGiath:AT2G25600.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ309323 Genomic DNA. Translation: CAC85283.1.
AC006053 Genomic DNA. Translation: AAD31377.1.
CP002685 Genomic DNA. Translation: AEC07722.1.
AK118279 mRNA. Translation: BAC42897.1.
PIRiD84650.
RefSeqiNP_180131.3. NM_128118.4.
UniGeneiAt.39001.

3D structure databases

ProteinModelPortaliQ8GXE6.
SMRiQ8GXE6. Positions 325-874.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2451. 7 interactions.
STRINGi3702.AT2G25600.1.

Protein family/group databases

TCDBi1.A.1.4.8. the voltage-gated ion channel (vic) superfamily.

Proteomic databases

PaxDbiQ8GXE6.
PRIDEiQ8GXE6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G25600.1; AT2G25600.1; AT2G25600.
GeneIDi817099.
GrameneiAT2G25600.1; AT2G25600.1; AT2G25600.
KEGGiath:AT2G25600.

Organism-specific databases

TAIRiAT2G25600.

Phylogenomic databases

eggNOGiKOG0498. Eukaryota.
ENOG410XPSE. LUCA.
HOGENOMiHOG000238230.
InParanoidiQ8GXE6.
OMAiHESIQGY.
PhylomeDBiQ8GXE6.

Enzyme and pathway databases

BioCyciARA:GQT-2555-MONOMER.
ReactomeiR-ATH-1296072. Voltage gated Potassium channels.

Miscellaneous databases

PROiQ8GXE6.

Gene expression databases

GenevisibleiQ8GXE6. AT.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR021789. KHA_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF11834. KHA. 1 hit.
[Graphical view]
PRINTSiPR01463. EAGCHANLFMLY.
SMARTiSM00248. ANK. 4 hits.
SM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS50042. CNMP_BINDING_3. 1 hit.
PS51490. KHA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Pollen tube development and competitive ability are impaired by disruption of a Shaker K(+) channel in Arabidopsis."
    Mouline K., Very A.-A., Gaymard F., Boucherez J., Pilot G., Devic M., Bouchez D., Thibaud J.-B., Sentenac H.
    Genes Dev. 16:339-350(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, TISSUE SPECIFICITY, FUNCTION.
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 407-888.
    Strain: cv. Columbia.
  5. "A shaker-like K(+) channel with weak rectification is expressed in both source and sink phloem tissues of Arabidopsis."
    Lacombe B., Pilot G., Michard E., Gaymard F., Sentenac H., Thibaud J.-B.
    Plant Cell 12:837-851(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiAKT6_ARATH
AccessioniPrimary (citable) accession number: Q8GXE6
Secondary accession number(s): Q9SLA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: February 17, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Was originally erroneously termed AKT5.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.