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Q8GXB7

- TRMB_ARATH

UniProt

Q8GXB7 - TRMB_ARATH

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Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
At5g24840, F6A4_50
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei71 – 711S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei148 – 1481S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Active sitei151 – 1511 By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
  2. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA (guanine-N7)-methylation Source: GOC
  2. tRNA modification Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

BioCyciARA:AT5G24840-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Ordered Locus Names:At5g24840
ORF Names:F6A4_50
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G24840.

Subcellular locationi

Nucleus By similarity UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
PRO_0000171435Add
BLAST

Proteomic databases

PaxDbiQ8GXB7.
PRIDEiQ8GXB7.

Expressioni

Gene expression databases

GenevestigatoriQ8GXB7.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G24840.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8GXB7.
SMRiQ8GXB7. Positions 39-247.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni94 – 952S-adenosyl-L-methionine binding By similarity
Regioni128 – 1292S-adenosyl-L-methionine binding By similarity
Regioni226 – 2283S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000260965.
InParanoidiQ8GXB7.
KOiK03439.
OMAiRAHSNPI.
PhylomeDBiQ8GXB7.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8GXB7-1 [UniParc]FASTAAdd to Basket

« Hide

MDNETKATTF SKSTGLPRKR FYRARAHSNP LSDSHFPIPI SPAHVDYSLH    50
FPKFVEADNK FIKKVEFADI GCGFGGLLIS LATLFPDTLM IGMELRDKVT 100
EYVKERILAL RRTSSEGQYE NISVVRTNSM KYIPNYFEKG QLSKMFFLFP 150
DPHFKEKNHR RRVISTHLLD EYAYVLRAGG IIYTITDVEE LGEWMKSCLE 200
KHPMFESLTQ EELVSDPVVE LLCSATEEGQ KVARNGGQTF RAVFRRIAYV 250
S 251
Length:251
Mass (Da):28,818
Last modified:March 1, 2003 - v1
Checksum:i8E603E3AE5FB4646
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF069716 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED93368.1.
AK118324 mRNA. Translation: BAC42938.1.
BT006128 mRNA. Translation: AAP04113.1.
RefSeqiNP_197866.1. NM_122393.3.
UniGeneiAt.45620.
At.69724.

Genome annotation databases

EnsemblPlantsiAT5G24840.1; AT5G24840.1; AT5G24840.
GeneIDi832553.
KEGGiath:AT5G24840.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF069716 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED93368.1 .
AK118324 mRNA. Translation: BAC42938.1 .
BT006128 mRNA. Translation: AAP04113.1 .
RefSeqi NP_197866.1. NM_122393.3.
UniGenei At.45620.
At.69724.

3D structure databases

ProteinModelPortali Q8GXB7.
SMRi Q8GXB7. Positions 39-247.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT5G24840.1-P.

Proteomic databases

PaxDbi Q8GXB7.
PRIDEi Q8GXB7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G24840.1 ; AT5G24840.1 ; AT5G24840 .
GeneIDi 832553.
KEGGi ath:AT5G24840.

Organism-specific databases

TAIRi AT5G24840.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000260965.
InParanoidi Q8GXB7.
KOi K03439.
OMAi RAHSNPI.
PhylomeDBi Q8GXB7.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci ARA:AT5G24840-MONOMER.

Miscellaneous databases

PROi Q8GXB7.

Gene expression databases

Genevestigatori Q8GXB7.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiTRMB_ARATH
AccessioniPrimary (citable) accession number: Q8GXB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: March 1, 2003
Last modified: June 11, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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