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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

At5g24840

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei71 – 711S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei148 – 1481S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Active sitei151 – 1511UniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: GO_Central
  2. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA (guanine-N7)-methylation Source: GO_Central
  2. tRNA methylation Source: GO_Central
  3. tRNA modification Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

BioCyciARA:AT5G24840-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Ordered Locus Names:At5g24840
ORF Names:F6A4_50
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G24840.

Subcellular locationi

Nucleus UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
  2. tRNA methyltransferase complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251tRNA (guanine-N(7)-)-methyltransferasePRO_0000171435Add
BLAST

Proteomic databases

PaxDbiQ8GXB7.
PRIDEiQ8GXB7.

Expressioni

Gene expression databases

GenevestigatoriQ8GXB7.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G24840.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8GXB7.
SMRiQ8GXB7. Positions 39-247.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni94 – 952S-adenosyl-L-methionine bindingUniRule annotation
Regioni128 – 1292S-adenosyl-L-methionine bindingUniRule annotation
Regioni226 – 2283S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000260965.
InParanoidiQ8GXB7.
KOiK03439.
OMAiMCTHFEE.
PhylomeDBiQ8GXB7.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8GXB7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNETKATTF SKSTGLPRKR FYRARAHSNP LSDSHFPIPI SPAHVDYSLH
60 70 80 90 100
FPKFVEADNK FIKKVEFADI GCGFGGLLIS LATLFPDTLM IGMELRDKVT
110 120 130 140 150
EYVKERILAL RRTSSEGQYE NISVVRTNSM KYIPNYFEKG QLSKMFFLFP
160 170 180 190 200
DPHFKEKNHR RRVISTHLLD EYAYVLRAGG IIYTITDVEE LGEWMKSCLE
210 220 230 240 250
KHPMFESLTQ EELVSDPVVE LLCSATEEGQ KVARNGGQTF RAVFRRIAYV

S
Length:251
Mass (Da):28,818
Last modified:February 28, 2003 - v1
Checksum:i8E603E3AE5FB4646
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069716 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED93368.1.
AK118324 mRNA. Translation: BAC42938.1.
BT006128 mRNA. Translation: AAP04113.1.
RefSeqiNP_197866.1. NM_122393.3.
UniGeneiAt.45620.
At.69724.

Genome annotation databases

EnsemblPlantsiAT5G24840.1; AT5G24840.1; AT5G24840.
GeneIDi832553.
KEGGiath:AT5G24840.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069716 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED93368.1.
AK118324 mRNA. Translation: BAC42938.1.
BT006128 mRNA. Translation: AAP04113.1.
RefSeqiNP_197866.1. NM_122393.3.
UniGeneiAt.45620.
At.69724.

3D structure databases

ProteinModelPortaliQ8GXB7.
SMRiQ8GXB7. Positions 39-247.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G24840.1-P.

Proteomic databases

PaxDbiQ8GXB7.
PRIDEiQ8GXB7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G24840.1; AT5G24840.1; AT5G24840.
GeneIDi832553.
KEGGiath:AT5G24840.

Organism-specific databases

TAIRiAT5G24840.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000260965.
InParanoidiQ8GXB7.
KOiK03439.
OMAiMCTHFEE.
PhylomeDBiQ8GXB7.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciARA:AT5G24840-MONOMER.

Miscellaneous databases

PROiQ8GXB7.

Gene expression databases

GenevestigatoriQ8GXB7.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiTRMB_ARATH
AccessioniPrimary (citable) accession number: Q8GXB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 19, 2003
Last sequence update: February 28, 2003
Last modified: February 3, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.