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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

At5g24840

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei71S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei148S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei151UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Ordered Locus Names:At5g24840
ORF Names:F6A4_50
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G24840
TAIRilocus:2149443 AT5G24840

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001714351 – 251tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST251

Proteomic databases

PaxDbiQ8GXB7
PRIDEiQ8GXB7

PTM databases

iPTMnetiQ8GXB7

Expressioni

Gene expression databases

ExpressionAtlasiQ8GXB7 baseline and differential
GenevisibleiQ8GXB7 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G24840.1

Structurei

3D structure databases

ProteinModelPortaliQ8GXB7
SMRiQ8GXB7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni94 – 95S-adenosyl-L-methionine bindingUniRule annotation2
Regioni128 – 129S-adenosyl-L-methionine bindingUniRule annotation2
Regioni226 – 228S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3115 Eukaryota
COG0220 LUCA
HOGENOMiHOG000260965
InParanoidiQ8GXB7
KOiK03439
OMAiIGMEIRV
OrthoDBiEOG09360OC8
PhylomeDBiQ8GXB7

Family and domain databases

HAMAPiMF_03055 tRNA_methyltr_TrmB_euk, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR025763 Trm8_euk
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
PTHR23417:SF16 PTHR23417:SF16, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

Q8GXB7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNETKATTF SKSTGLPRKR FYRARAHSNP LSDSHFPIPI SPAHVDYSLH
60 70 80 90 100
FPKFVEADNK FIKKVEFADI GCGFGGLLIS LATLFPDTLM IGMELRDKVT
110 120 130 140 150
EYVKERILAL RRTSSEGQYE NISVVRTNSM KYIPNYFEKG QLSKMFFLFP
160 170 180 190 200
DPHFKEKNHR RRVISTHLLD EYAYVLRAGG IIYTITDVEE LGEWMKSCLE
210 220 230 240 250
KHPMFESLTQ EELVSDPVVE LLCSATEEGQ KVARNGGQTF RAVFRRIAYV

S
Length:251
Mass (Da):28,818
Last modified:March 1, 2003 - v1
Checksum:i8E603E3AE5FB4646
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069716 Genomic DNA No translation available.
CP002688 Genomic DNA Translation: AED93368.1
AK118324 mRNA Translation: BAC42938.1
BT006128 mRNA Translation: AAP04113.1
RefSeqiNP_197866.1, NM_122393.3
UniGeneiAt.45620
At.69724

Genome annotation databases

EnsemblPlantsiAT5G24840.1; AT5G24840.1; AT5G24840
GeneIDi832553
GrameneiAT5G24840.1; AT5G24840.1; AT5G24840
KEGGiath:AT5G24840

Similar proteinsi

Entry informationi

Entry nameiTRMB_ARATH
AccessioniPrimary (citable) accession number: Q8GXB7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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