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Protein

Probable pectinesterase/pectinesterase inhibitor 21

Gene

PME21

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Acts in the modification of cell walls via demethylesterification of cell wall pectin.By similarity

Catalytic activityi

Pectin + n H2O = n methanol + pectate.

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Probable pectinesterase/pectinesterase inhibitor 7 (PME7), Pectinesterase (AXX17_At1g11890), Pectinesterase (AXX17_At4g02940), Pectinesterase (AXX17_At5g52320), Probable pectinesterase/pectinesterase inhibitor 42 (PME42), Probable pectinesterase 15 (PME15), Putative pectinesterase 14 (PME14), Pectinesterase (At3g10720), Pectinesterase (AXX17_At2g33410), Probable pectinesterase 55 (PME55), Probable pectinesterase/pectinesterase inhibitor 23 (PME23), Pectinesterase (AXX17_At4g18720), Pectinesterase 4 (PME4), Putative pectinesterase/pectinesterase inhibitor 22 (PME22), Pectinesterase (AXX17_At1g48290), Pectinesterase (AXX17_At5g47900), Pectinesterase (At3g14310), Pectinesterase (AXX17_At4g38050), Pectinesterase (AXX17_At5g07100), Pectinesterase/pectinesterase inhibitor 18 (PME18), Pectinesterase (AXX17_At2g14510), Pectinesterase (AXX17_At3g43380), Pectinesterase (AXX17_At5g64270), Probable pectinesterase/pectinesterase inhibitor 64 (PME64), Putative pectinesterase 10 (PME10), Pectinesterase (F14I3.7), Probable pectinesterase 29 (PME29), Pectinesterase, Pectinesterase (AXX17_At4g38040), Probable pectinesterase 8 (PME8), Pectinesterase (AXX17_At5g27870), Pectinesterase (AXX17_At5g18820), Pectinesterase (AXX17_At2g45340), Pectinesterase 31 (PME31), Pectinesterase (AXX17_At3g41380), Pectinesterase (AXX17_At1g11650), Probable pectinesterase/pectinesterase inhibitor 25 (PME25), Probable pectinesterase/pectinesterase inhibitor 51 (PME51), Pectinesterase (AXX17_At3g36460), Pectinesterase (AXX17_At5g04430), Pectinesterase (At3g62170), Probable pectinesterase/pectinesterase inhibitor 20 (PME20), Pectinesterase (T27B3.30), Probable pectinesterase/pectinesterase inhibitor 60 (PME60), Probable pectinesterase/pectinesterase inhibitor 59 (PME59), Pectinesterase QRT1 (QRT1), Pectinesterase (AXX17_At3g53530), Pectinesterase (AXX17_At5g61190), Pectinesterase PPME1 (PPME1), Pectinesterase (AXX17_At3g56460), Pectinesterase (AXX17_At3g06710), Probable pectinesterase/pectinesterase inhibitor 32 (PME32), Pectinesterase (AXX17_At1g44540), Probable pectinesterase/pectinesterase inhibitor 33 (PME33), Probable pectinesterase/pectinesterase inhibitor 36 (PME36), Probable pectinesterase/pectinesterase inhibitor 13 (PME13), Putative pectinesterase 52 (PME52), Probable pectinesterase/pectinesterase inhibitor 54 (PME54), Pectinesterase (AXX17_At2g33420), Pectinesterase (AXX17_At2g40510), Pectinesterase (At1g53840), Pectinesterase 5 (PME5), Probable pectinesterase/pectinesterase inhibitor 16 (PME16), Probable pectinesterase 30 (PME30), Probable pectinesterase/pectinesterase inhibitor VGDH2 (VGDH2), Pectinesterase (AXX17_At3g26070), Probable pectinesterase 68 (PME68), Pectinesterase, Pectinesterase (AXX17_At3g14900), Pectinesterase (AXX17_At5g54740), Probable pectinesterase 67 (PME67), Putative pectinesterase/pectinesterase inhibitor 38 (PME38), Probable pectinesterase 56 (PME56), Pectinesterase, Probable pectinesterase/pectinesterase inhibitor 47 (PME47), Putative pectinesterase/pectinesterase inhibitor 28 (PME28), Probable pectinesterase 53 (PME53), Probable pectinesterase/pectinesterase inhibitor 46 (PME46), Pectinesterase (AXX17_At5g07090), Probable pectinesterase/pectinesterase inhibitor 17 (PME17), Pectinesterase (AXX17_At2g22270), Probable pectinesterase/pectinesterase inhibitor 61 (PME61), Probable pectinesterase 66 (PME66), Pectinesterase (AXX17_At2g42790), Pectinesterase (AXX17_At5g45990), Probable pectinesterase/pectinesterase inhibitor 6 (PME6), Probable pectinesterase 48 (PME48), Probable pectinesterase 49 (PME49), Pectinesterase (AXX17_At1g64100), Probable pectinesterase 50 (PME50), Pectinesterase (AXX17_At3g17940), Pectinesterase, Probable pectinesterase/pectinesterase inhibitor 19 (PME19), Pectinesterase (AXX17_At2g45050), Probable pectinesterase/pectinesterase inhibitor 40 (PME40), Pectinesterase (AXX17_At1g11920), Pectinesterase (AXX17_At3g30550), PME26 (AXX17_At3g14890), Probable pectinesterase/pectinesterase inhibitor 39 (PME39), Pectinesterase (AXX17_At2g44790), Pectinesterase (AXX17_At2g17140), Pectinesterase (AXX17_At5g19650), Putative pectinesterase/pectinesterase inhibitor 43 (PME43), Pectinesterase (AXX17_At3g31890), Pectinesterase (AXX17_At4g02900), Probable pectinesterase/pectinesterase inhibitor 34 (PME34), Pectinesterase 1 (PME1), Putative pectinesterase 63 (PME63), Pectinesterase (AXX17_At3g05180), Pectinesterase 2 (PME2), Probable pectinesterase/pectinesterase inhibitor 21 (PME21), Pectinesterase (AXX17_At2g22280), Pectinesterase (AXX17_At1g51200), Putative pectinesterase/pectinesterase inhibitor 45 (PME45), Probable pectinesterase/pectinesterase inhibitor 12 (PME12), Pectinesterase (AXX17_At4g02930), Probable pectinesterase/pectinesterase inhibitor 44 (PME44), Pectinesterase, Pectinesterase/pectinesterase inhibitor 3 (PME3), Pectinesterase (AXX17_At1g24390), Pectinesterase (AXX17_At5g50300), Pectinesterase (AXX17_At1g11900), Probable pectinesterase/pectinesterase inhibitor 58 (PME58), Putative pectinesterase 57 (PME57), Pectinesterase (AXX17_At3g10560), Pectinesterase (AXX17_At3g55110), Pectinesterase (AXX17_At3g10570), Putative pectinesterase 11 (PME11), Pectinesterase (AXX17_At1g01970), Pectinesterase (AXX17_At5g07110), Pectinesterase (AXX17_At4g06570), Pectinesterase (AXX17_At5g50310), Pectinesterase, Pectinesterase (AXX17_At1g51190), Pectinesterase (AXX17_At2g33420), Pectinesterase (AXX17_At1g48300), Pectinesterase (AXX17_At3g05190), Putative pectinesterase/pectinesterase inhibitor 24 (PME24), Putative pectinesterase/pectinesterase inhibitor 26 (PME26), Probable pectinesterase/pectinesterase inhibitor 35 (PME35), Pectinesterase (AXX17_At5g04420), Pectinesterase (AXX17_At2g44780), Pectinesterase (AXX17_At4g00230), Pectinesterase (AXX17_At5g09310), Pectinesterase (AXX17_At5g20800), Pectinesterase (AXX17_At1g04730), Probable pectinesterase/pectinesterase inhibitor 41 (PME41), Pectinesterase (AXX17_At1g11660), Pectinesterase (AXX17_At5g26780)
  2. Pectate lyase (AXX17_At3g26720), Probable pectate lyase 7 (At3g01270), Probable pectate lyase 18 (At4g24780), Probable pectate lyase 16 (At4g22080), Putative pectate lyase 17 (At4g22090), Pectate lyase (AXX17_At3g09180), Putative pectate lyase 14 (At4g13210), Pectate lyase (At3g55140), Pectate lyase (AXX17_At5g54880), Pectate lyase (AXX17_At4g15660), Probable pectate lyase 22 (At5g63180), Pectate lyase (T5E8_80), Pectate lyase (AXX17_At3g00310), Pectate lyase (At3g07010), Probable pectate lyase 8 (At3g07010), Probable pectate lyase 4 (At1g30350), Pectate lyase (AXX17_At2g01680), Putative pectate lyase 21 (At5g55720), Pectate lyase (At4g13210), Pectate lyase (At5g04310), Pectate lyase (AXX17_At5g03700), Pectate lyase (At4g13710), Pectate lyase (AXX17_At3g49720), Pectate lyase (At3g01270), Pectate lyase (At5g04310), Pectate lyase (AXX17_At1g12300), Probable pectate lyase 9 (At3g24230), Probable pectate lyase 1 (At1g04680), Pectate lyase (AXX17_At5g08800), Pectate lyase (T26I12.20), Pectate lyase (AXX17_At5g62760), Pectate lyase (AXX17_At3g47590), Pectate lyase (AXX17_At4g14900), Pectate lyase (AXX17_At3g49450), Pectate lyase (AXX17_At4g25600), Pectate lyase (AXX17_At1g61710), Probable pectate lyase 20 (At5g48900), Pectate lyase (AXX17_At5g47570), Probable pectate lyase 6 (At2g02720), Pectate lyase (AXX17_At3g06910), Pectate lyase (At1g14420), Pectate lyase (At3g01270), Pectate lyase (At2g02720), Pectate lyase (AXX17_At1g04020), Pectate lyase (At3g01270), Pectate lyase (At3g55140), Putative pectate lyase 2 (At1g11920), Pectate lyase, Pectate lyase (AXX17_At1g30780), Probable pectate lyase 19 (At5g15110), Pectate lyase (F11F8_12), Probable pectate lyase 13 (PMR6), Probable pectate lyase 3 (AT59), Pectate lyase (AXX17_At3g26200), Probable pectate lyase 5 (At1g67750), Probable pectate lyase 12 (At3g53190), Pectate lyase (At4g24780), Pectate lyase (AXX17_At4g25610), Probable pectate lyase 15 (At4g13710), Pectate lyase (At3g53190), Putative pectate lyase 11 (At3g27400), Pectate lyase (AXX17_At1g15010), Pectate lyase (At3g07010), Pectate lyase (AXX17_At3g29900), Pectate lyase (AXX17_At5g14600), Probable pectate lyase 10 (At3g24670)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei330Substrate; for pectinesterase activityBy similarity1
Binding sitei360Substrate; for pectinesterase activityBy similarity1
Sitei382Transition state stabilizerBy similarity1
Active sitei383Proton donor; for pectinesterase activityBy similarity1
Active sitei404Nucleophile; for pectinesterase activityBy similarity1
Binding sitei472Substrate; for pectinesterase activityBy similarity1
Binding sitei474Substrate; for pectinesterase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl esterase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT3G05610-MONOMER.
UniPathwayiUPA00545; UER00823.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable pectinesterase/pectinesterase inhibitor 21
Including the following 2 domains:
Pectinesterase inhibitor 21
Alternative name(s):
Pectin methylesterase inhibitor 21
Pectinesterase 21 (EC:3.1.1.11)
Short name:
PE 21
Alternative name(s):
Pectin methylesterase 21
Short name:
AtPME21
Gene namesi
Name:PME21
Synonyms:ARATH21
Ordered Locus Names:At3g05610
ORF Names:F18C1.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G05610.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei16 – 36HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003716771 – 669Probable pectinesterase/pectinesterase inhibitor 21Add BLAST669

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi52N-linked (GlcNAc...)Sequence analysis1
Glycosylationi81N-linked (GlcNAc...)Sequence analysis1
Glycosylationi94N-linked (GlcNAc...)Sequence analysis1
Glycosylationi281N-linked (GlcNAc...)Sequence analysis1
Glycosylationi300N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi397 ↔ 417By similarity
Glycosylationi416N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8GX86.
PRIDEiQ8GX86.

Expressioni

Tissue specificityi

Expressed in flower buds.1 Publication

Gene expression databases

GenevisibleiQ8GX86. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G05610.1.

Structurei

3D structure databases

ProteinModelPortaliQ8GX86.
SMRiQ8GX86.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni52 – 205Pectinesterase inhibitor 21Add BLAST154
Regioni255 – 551Pectinesterase 21Add BLAST297

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi245 – 248Poly-Ala4
Compositional biasi603 – 666Ser-richAdd BLAST64

Sequence similaritiesi

In the N-terminal section; belongs to the PMEI family.Curated
In the C-terminal section; belongs to the pectinesterase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IGWD. Eukaryota.
COG4677. LUCA.
HOGENOMiHOG000217409.
InParanoidiQ8GX86.
KOiK01051.
OMAiKNTTDPM.
OrthoDBiEOG093609DH.
PhylomeDBiQ8GX86.

Family and domain databases

Gene3Di1.20.140.40. 1 hit.
2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib_dom.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view]
SMARTiSM00856. PMEI. 1 hit.
[Graphical view]
SUPFAMiSSF101148. SSF101148. 1 hit.
SSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR01614. PME_inhib. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8GX86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYGYDDESK RKRRYIVITI SSVLLISMVV AVTVGVSLNK HDGDSKGKAE
60 70 80 90 100
VNASVKAVKD VCAPTDYRKT CEDTLIKNGK NTTDPMELVK TAFNVTMKQI
110 120 130 140 150
TDAAKKSQTI MELQKDSRTR MALDQCKELM DYALDELSNS FEELGKFEFH
160 170 180 190 200
LLDEALINLR IWLSAAISHE ETCLEGFQGT QGNAGETMKK ALKTAIELTH
210 220 230 240 250
NGLAIISEMS NFVGQMQIPG LNSRRLLAEG FPSWVDQRGR KLLQAAAAYS
260 270 280 290 300
DVKPDIVVAQ DGSGQYKTIN EALQFVPKKR NTTFVVHIKA GLYKEYVQVN
310 320 330 340 350
KTMSHLVFIG DGPDKTIISG NKNYKDGITT YRTATVAIVG NYFIAKNIGF
360 370 380 390 400
ENTAGAIKHQ AVAVRVQSDE SIFFNCRFDG YQDTLYTHSH RQFFRDCTIS
410 420 430 440 450
GTIDFLFGDA AAVFQNCTLL VRKPLPNQAC PITAHGRKDP RESTGFVFQG
460 470 480 490 500
CTIAGEPDYL AVKETSKAYL GRPWKEYSRT IIMNTFIPDF VQPQGWQPWL
510 520 530 540 550
GDFGLKTLFY SEVQNTGPGS ALANRVTWAG IKTLSEEDIL KFTPAQYIQG
560 570 580 590 600
DDWIPGKGVP YTTGLLAGNP AAATTTPSVS AAAPGFSTFT DTSGADSIAP
610 620 630 640 650
TASPAASPES SISMAYTGTA SPESSIKVSS STETASPESS FTEASTASPE
660
SSIMVASTES SGSFFSMFT
Length:669
Mass (Da):72,917
Last modified:May 5, 2009 - v2
Checksum:i2D7DC0FF415EB573
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti330T → A in BAC42986 (PubMed:11910074).Curated1
Sequence conflicti330T → A in AAO64883 (PubMed:14593172).Curated1
Sequence conflicti383D → N in BAC42986 (PubMed:11910074).Curated1
Sequence conflicti383D → N in AAO64883 (PubMed:14593172).Curated1
Sequence conflicti630S → F in BAC42986 (PubMed:11910074).Curated1
Sequence conflicti630S → F in AAO64883 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC011620 Genomic DNA. Translation: AAF26136.1.
CP002686 Genomic DNA. Translation: AEE74266.1.
AK118374 mRNA. Translation: BAC42986.1.
BT005948 mRNA. Translation: AAO64883.1.
RefSeqiNP_187212.1. NM_111434.3.
UniGeneiAt.40644.

Genome annotation databases

EnsemblPlantsiAT3G05610.1; AT3G05610.1; AT3G05610.
GeneIDi819727.
GrameneiAT3G05610.1; AT3G05610.1; AT3G05610.
KEGGiath:AT3G05610.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC011620 Genomic DNA. Translation: AAF26136.1.
CP002686 Genomic DNA. Translation: AEE74266.1.
AK118374 mRNA. Translation: BAC42986.1.
BT005948 mRNA. Translation: AAO64883.1.
RefSeqiNP_187212.1. NM_111434.3.
UniGeneiAt.40644.

3D structure databases

ProteinModelPortaliQ8GX86.
SMRiQ8GX86.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G05610.1.

Proteomic databases

PaxDbiQ8GX86.
PRIDEiQ8GX86.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G05610.1; AT3G05610.1; AT3G05610.
GeneIDi819727.
GrameneiAT3G05610.1; AT3G05610.1; AT3G05610.
KEGGiath:AT3G05610.

Organism-specific databases

TAIRiAT3G05610.

Phylogenomic databases

eggNOGiENOG410IGWD. Eukaryota.
COG4677. LUCA.
HOGENOMiHOG000217409.
InParanoidiQ8GX86.
KOiK01051.
OMAiKNTTDPM.
OrthoDBiEOG093609DH.
PhylomeDBiQ8GX86.

Enzyme and pathway databases

UniPathwayiUPA00545; UER00823.
BioCyciARA:AT3G05610-MONOMER.

Miscellaneous databases

PROiQ8GX86.

Gene expression databases

GenevisibleiQ8GX86. AT.

Family and domain databases

Gene3Di1.20.140.40. 1 hit.
2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib_dom.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view]
SMARTiSM00856. PMEI. 1 hit.
[Graphical view]
SUPFAMiSSF101148. SSF101148. 1 hit.
SSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR01614. PME_inhib. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPME21_ARATH
AccessioniPrimary (citable) accession number: Q8GX86
Secondary accession number(s): Q9M9W6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: November 30, 2016
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.