Beta-galactosidase 16 precursor - Arabidopsis thaliana (Mouse-ear cress)

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Q8GX69 (BGA16_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 76. History...

Clusters with 100%, 90%, 50% identity |

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Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Beta-galactosidase 16
Short name=Lactase 16
EC=3.2.1.23

Gene names

Name:	BGAL16
Ordered Locus Names:	At1g77410
ORF Names:	F2P24.12, T5M16.1

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	815 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subcellular location	Secreted › extracellular space › apoplast Potential.
Tissue specificity	Ubiquitous, with higher expression levels in siliques. Ref.5
Sequence similarities	Belongs to the glycosyl hydrolase 35 family. Contains 1 SUEL-type lectin domain.
Sequence caution	The sequence AAG29193.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAO64909.1 differs from that shown. Reason: Frameshift at position 798. The sequence BAC43014.1 differs from that shown. Reason: Frameshift at position 798.

Ontologies

Keywords
Cellular component	Apoplast Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta-galactosidase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

23

Potential

Chain

792

Beta-galactosidase 16

PRO_0000293095

Regions

Domain

87

SUEL-type lectin

Sites

Active site

1

Proton donor Potential

Active site

1

Nucleophile Potential

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q8GX69 [UniParc].

Last modified July 10, 2007. Version 2.
Checksum: 47B97FBC710A0508
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815

91,648

        10         20         30         40         50         60 
MTTFQYSLVF LVLMAVIVAG DVANVTYDGR SLIIDGEHKI LFSGSIHYTR STPQMWPSLI 

        70         80         90        100        110        120 
AKAKSGGIDV VDTYVFWNVH EPQQGQFDFS GSRDIVKFIK EVKNHGLYVC LRIGPFIQGE 

       130        140        150        160        170        180 
WSYGGLPFWL HNVQGIVFRT DNEPFKYHMK RYAKMIVKLM KSENLYASQG GPIILSQIEN 

       190        200        210        220        230        240 
EYGMVGRAFR QEGKSYVKWT AKLAVELDTG VPWVMCKQDD APDPLVNACN GRQCGETFKG 

       250        260        270        280        290        300 
PNSPNKPAIW TENWTSFYQT YGEEPLIRSA EDIAFHVALF IAKNGSFVNY YMYHGGTNFG 

       310        320        330        340        350        360 
RNASQFVITS YYDQAPLDEY GLLRQPKWGH LKELHAAVKL CEEPLLSGLQ TTISLGKLQT 

       370        380        390        400        410        420 
AFVFGKKANL CAAILVNQDK CESTVQFRNS SYRLSPKSVS VLPDCKNVAF NTAKVNAQYN 

       430        440        450        460        470        480 
TRTRKARQNL SSPQMWEEFT ETVPSFSETS IRSESLLEHM NTTQDTSDYL WQTTRFQQSE 

       490        500        510        520        530        540 
GAPSVLKVNH LGHALHAFVN GRFIGSMHGT FKAHRFLLEK NMSLNNGTNN LALLSVMVGL 

       550        560        570        580        590        600 
PNSGAHLERR VVGSRSVKIW NGRYQLYFNN YSWGYQVGLK GEKFHVYTED GSAKVQWKQY 

       610        620        630        640        650        660 
RDSKSQPLTW YKASFDTPEG EDPVALNLGS MGKGEAWVNG QSIGRYWVSF HTYKGNPSQI 

       670        680        690        700        710        720 
WYHIPRSFLK PNSNLLVILE EEREGNPLGI TIDTVSVTEV CGHVSNTNPH PVISPRKKGL 

       730        740        750        760        770        780 
NRKNLTYRYD RKPKVQLQCP TGRKISKILF ASFGTPNGSC GSYSIGSCHS PNSLAVVQKA 

       790        800        810 
CLKKSRCSVP VWSKTFGGDS CPHTVKSLLV RAQCS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana." Iglesias N., Abelenda J.A., Rodino M., Sampedro J., Revilla G., Zarra I. Plant Cell Physiol. 47:55-63(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35." Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C. Phytochemistry 68:1510-1520(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC010704 Genomic DNA. Translation: AAG51670.1. AC078898 Genomic DNA. Translation: AAG29193.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE35975.1. AK118405 mRNA. Translation: BAC43014.1. Frameshift. BT005974 mRNA. Translation: AAO64909.1. Frameshift.
IPI	IPI00543346.
PIR	D96803.
RefSeq	NP_177866.2. NM_106391.2.
UniGene	At.34478.
3D structure databases
ProteinModelPortal	Q8GX69.
SMR	Q8GX69. Positions 31-334, 598-680.
ModBase	Search...
Protein family/group databases
CAZy	GH35. Glycoside Hydrolase Family 35.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G77410.1; AT1G77410.1; AT1G77410.
GeneID	844078.
KEGG	ath:AT1G77410.
Organism-specific databases
GeneFarm	847. 90.
TAIR	At1g77410.
Phylogenomic databases
eggNOG	COG1874.
HOGENOM	HOG000239919.
InParanoid	Q8GX69.
OMA	GNDAVTL.
PhylomeDB	Q8GX69.
ProtClustDB	CLSN2680658.
Gene expression databases
Genevestigator	Q8GX69.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR008979. Galactose-bd-like. IPR019801. Glyco_hydro_35_CS. IPR013781. Glyco_hydro_catalytic_dom. IPR001944. Glycoside_Hdrlase_35. IPR017853. Glycoside_hydrolase_SF. IPR000922. Lectin_gal-bd_dom. [Graphical view]
PANTHER	PTHR23421. PTHR23421. 1 hit.
Pfam	PF02140. Gal_Lectin. 1 hit. PF01301. Glyco_hydro_35. 1 hit. [Graphical view]
PRINTS	PR00742. GLHYDRLASE35.
SUPFAM	SSF49785. Gal_bind_like. 2 hits. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS01182. GLYCOSYL_HYDROL_F35. 1 hit. PS00307. LECTIN_LEGUME_BETA. 1 hit. PS50228. SUEL_LECTIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGA16_ARATH

Accession

Primary (citable) accession number: Q8GX69
Secondary accession number(s): Q9CAR2, Q9FVW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	May 1, 2013
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents