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Q8GX69 (BGA16_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase 16

Short name=Lactase 16
EC=3.2.1.23
Gene names
Name:BGAL16
Ordered Locus Names:At1g77410
ORF Names:F2P24.12, T5M16.1
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length815 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subcellular location

Secretedextracellular spaceapoplast Potential.

Tissue specificity

Ubiquitous, with higher expression levels in siliques. Ref.5

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Contains 1 SUEL-type lectin domain.

Sequence caution

The sequence AAG29193.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence AAO64909.1 differs from that shown. Reason: Frameshift at position 798.

The sequence BAC43014.1 differs from that shown. Reason: Frameshift at position 798.

Ontologies

Keywords
   Cellular componentApoplast
Secreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 815792Beta-galactosidase 16
PRO_0000293095

Regions

Domain729 – 81587SUEL-type lectin

Sites

Active site1811Proton donor Potential
Active site2521Nucleophile Potential

Amino acid modifications

Glycosylation241N-linked (GlcNAc...) Potential
Glycosylation2531N-linked (GlcNAc...) Potential
Glycosylation2841N-linked (GlcNAc...) Potential
Glycosylation3021N-linked (GlcNAc...) Potential
Glycosylation3891N-linked (GlcNAc...) Potential
Glycosylation4291N-linked (GlcNAc...) Potential
Glycosylation4611N-linked (GlcNAc...) Potential
Glycosylation5211N-linked (GlcNAc...) Potential
Glycosylation5261N-linked (GlcNAc...) Potential
Glycosylation5701N-linked (GlcNAc...) Potential
Glycosylation7241N-linked (GlcNAc...) Potential
Glycosylation7571N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q8GX69 [UniParc].

Last modified July 10, 2007. Version 2.
Checksum: 47B97FBC710A0508

FASTA81591,648
        10         20         30         40         50         60 
MTTFQYSLVF LVLMAVIVAG DVANVTYDGR SLIIDGEHKI LFSGSIHYTR STPQMWPSLI 

        70         80         90        100        110        120 
AKAKSGGIDV VDTYVFWNVH EPQQGQFDFS GSRDIVKFIK EVKNHGLYVC LRIGPFIQGE 

       130        140        150        160        170        180 
WSYGGLPFWL HNVQGIVFRT DNEPFKYHMK RYAKMIVKLM KSENLYASQG GPIILSQIEN 

       190        200        210        220        230        240 
EYGMVGRAFR QEGKSYVKWT AKLAVELDTG VPWVMCKQDD APDPLVNACN GRQCGETFKG 

       250        260        270        280        290        300 
PNSPNKPAIW TENWTSFYQT YGEEPLIRSA EDIAFHVALF IAKNGSFVNY YMYHGGTNFG 

       310        320        330        340        350        360 
RNASQFVITS YYDQAPLDEY GLLRQPKWGH LKELHAAVKL CEEPLLSGLQ TTISLGKLQT 

       370        380        390        400        410        420 
AFVFGKKANL CAAILVNQDK CESTVQFRNS SYRLSPKSVS VLPDCKNVAF NTAKVNAQYN 

       430        440        450        460        470        480 
TRTRKARQNL SSPQMWEEFT ETVPSFSETS IRSESLLEHM NTTQDTSDYL WQTTRFQQSE 

       490        500        510        520        530        540 
GAPSVLKVNH LGHALHAFVN GRFIGSMHGT FKAHRFLLEK NMSLNNGTNN LALLSVMVGL 

       550        560        570        580        590        600 
PNSGAHLERR VVGSRSVKIW NGRYQLYFNN YSWGYQVGLK GEKFHVYTED GSAKVQWKQY 

       610        620        630        640        650        660 
RDSKSQPLTW YKASFDTPEG EDPVALNLGS MGKGEAWVNG QSIGRYWVSF HTYKGNPSQI 

       670        680        690        700        710        720 
WYHIPRSFLK PNSNLLVILE EEREGNPLGI TIDTVSVTEV CGHVSNTNPH PVISPRKKGL 

       730        740        750        760        770        780 
NRKNLTYRYD RKPKVQLQCP TGRKISKILF ASFGTPNGSC GSYSIGSCHS PNSLAVVQKA 

       790        800        810 
CLKKSRCSVP VWSKTFGGDS CPHTVKSLLV RAQCS 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Functional annotation of a full-length Arabidopsis cDNA collection."
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.
Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana."
Iglesias N., Abelenda J.A., Rodino M., Sampedro J., Revilla G., Zarra I.
Plant Cell Physiol. 47:55-63(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35."
Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C.
Phytochemistry 68:1510-1520(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC010704 Genomic DNA. Translation: AAG51670.1.
AC078898 Genomic DNA. Translation: AAG29193.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE35975.1.
AK118405 mRNA. Translation: BAC43014.1. Frameshift.
BT005974 mRNA. Translation: AAO64909.1. Frameshift.
PIRD96803.
RefSeqNP_177866.2. NM_106391.2.
UniGeneAt.34478.

3D structure databases

ProteinModelPortalQ8GX69.
SMRQ8GX69. Positions 21-699, 733-815.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH35. Glycoside Hydrolase Family 35.

Proteomic databases

PRIDEQ8GX69.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G77410.1; AT1G77410.1; AT1G77410.
GeneID844078.
KEGGath:AT1G77410.

Organism-specific databases

GeneFarm847. 90.
TAIRAT1G77410.

Phylogenomic databases

eggNOGCOG1874.
HOGENOMHOG000239919.
InParanoidQ8GX69.
OMAGNDAVTL.
PhylomeDBQ8GX69.
ProtClustDBCLSN2680658.

Enzyme and pathway databases

BioCycARA:AT1G77410-MONOMER.

Gene expression databases

GenevestigatorQ8GX69.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERPTHR23421. PTHR23421. 1 hit.
PfamPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSPR00742. GLHYDRLASE35.
SUPFAMSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS00307. LECTIN_LEGUME_BETA. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGA16_ARATH
AccessionPrimary (citable) accession number: Q8GX69
Secondary accession number(s): Q9CAR2, Q9FVW7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: March 19, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names