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Protein

Chaperone protein dnaJ GFA2, mitochondrial

Gene

GFA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Chaperone that may play a role in mitochondrial protein folding. Involved in female gametophyte development. Required for cell death of the synergid cells during fertilization process, and fusion of the polar nuclei during megagametogenesis.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi238Zinc 1By similarity1
Metal bindingi241Zinc 1By similarity1
Metal bindingi255Zinc 2By similarity1
Metal bindingi258Zinc 2By similarity1
Metal bindingi277Zinc 2By similarity1
Metal bindingi280Zinc 2By similarity1
Metal bindingi291Zinc 1By similarity1
Metal bindingi294Zinc 1By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri225 – 303CR-typePROSITE-ProRule annotationAdd BLAST79

GO - Molecular functioni

GO - Biological processi

  • chaperone mediated protein folding requiring cofactor Source: TAIR
  • embryo sac cellularization Source: TAIR
  • embryo sac development Source: TAIR
  • nuclear membrane fusion Source: TAIR
  • polar nucleus fusion Source: TAIR
  • response to heat Source: InterPro
  • synergid death Source: TAIR

Keywordsi

Molecular functionChaperone
Biological processApoptosis
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein dnaJ GFA2, mitochondrialCurated
Alternative name(s):
Chaperone protein dnaJ A301 Publication
Short name:
AtDjA301 Publication
Gametophytic factor 21 Publication
Gene namesi
Name:GFA21 Publication
Ordered Locus Names:At5g48030Imported
ORF Names:MDN11.11Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G48030.
TAIRilocus:2162692. AT5G48030.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Defect in fusion of polar nuclei and in synergid cell death.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 89MitochondrionSequence analysisAdd BLAST89
ChainiPRO_000043572890 – 456Chaperone protein dnaJ GFA2, mitochondrialSequence analysisAdd BLAST367

Proteomic databases

PaxDbiQ8GWW8.

PTM databases

SwissPalmiQ8GWW8.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

ExpressionAtlasiQ8GWW8. baseline and differential.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi3702.AT5G48030.1.

Structurei

3D structure databases

ProteinModelPortaliQ8GWW8.
SMRiQ8GWW8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini94 – 159JPROSITE-ProRule annotationAdd BLAST66
Repeati238 – 245CXXCXGXG motifCurated8
Repeati255 – 262CXXCXGXG motifCurated8
Repeati277 – 284CXXCXGXG motifCurated8
Repeati291 – 298CXXCXGXG motifCurated8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi169 – 184Gly-richPROSITE-ProRule annotationAdd BLAST16

Sequence similaritiesi

Belongs to the DnaJ family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri225 – 303CR-typePROSITE-ProRule annotationAdd BLAST79

Keywords - Domaini

Repeat, Transit peptide, Zinc-finger

Phylogenomic databases

eggNOGiKOG0715. Eukaryota.
COG0484. LUCA.
HOGENOMiHOG000226717.
KOiK03686.
OMAiASKSGHD.
OrthoDBiEOG093609NW.
PhylomeDBiQ8GWW8.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
cd10719. DnaJ_zf. 1 hit.
Gene3Di1.10.287.110. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiView protein in InterPro
IPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR036869. DnaJ_dom_sf.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
IPR036410. HSP_DnaJ_Cys-rich_dom_sf.
PfamiView protein in Pfam
PF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
PRINTSiPR00625. JDOMAIN.
SMARTiView protein in SMART
SM00271. DnaJ. 1 hit.
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEiView protein in PROSITE
PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8GWW8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVPSNGAKVL RLLSRRCLSS SLIQDLANQK LRGVCIGSYR RLNTSVGNHA
60 70 80 90 100
NVIGDYASKS GHDRKWINFG GFNTNFGSTR SFHGTGSSFM SAKDYYSVLG
110 120 130 140 150
VSKNAQEGEI KKAYYGLAKK LHPDMNKDDP EAETKFQEVS KAYEILKDKE
160 170 180 190 200
KRDLYDQVGH EAFEQNASGG FPNDQGFGGG GGGGFNPFDI FGSFNGDIFN
210 220 230 240 250
MYRQDIGGQD VKVLLDLSFM EAVQGCSKTV TFQTEMACNT CGGQGVPPGT
260 270 280 290 300
KREKCKACNG SGMTSLRRGM LSIQTTCQKC GGAGQTFSSI CKSCRGARVV
310 320 330 340 350
RGQKSVKVTI DPGVDNSDTL KVARVGGADP EGDQPGDLYV TLKVREDPVF
360 370 380 390 400
RREGSDIHVD AVLSVTQAIL GGTIQVPTLT GDVVVKVRPG TQPGHKVVLR
410 420 430 440 450
NKGIRARKST KFGDQYVHFN VSIPANITQR QRELLEEFSK AEQGEYEQRT

ATGSSQ
Length:456
Mass (Da):49,438
Last modified:March 1, 2003 - v1
Checksum:iAD571ED32BE33D2F
GO

Sequence cautioni

The sequence BAB11067 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30K → R in AAM62460 (Ref. 5) Curated1
Sequence conflicti63D → H in AAM62460 (Ref. 5) Curated1
Sequence conflicti158V → F in AAM62460 (Ref. 5) Curated1
Sequence conflicti174D → Y in AAM62460 (Ref. 5) Curated1
Sequence conflicti235E → Q in AAM49801 (PubMed:12215516).Curated1
Sequence conflicti309T → N in AAM62460 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY103490 mRNA. Translation: AAM49801.1.
AB017064 Genomic DNA. Translation: BAB11067.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED95612.1.
AK118588 mRNA. Translation: BAC43188.1.
AY085227 mRNA. Translation: AAM62460.1.
RefSeqiNP_568690.1. NM_124177.4.
UniGeneiAt.28769.

Genome annotation databases

EnsemblPlantsiAT5G48030.1; AT5G48030.1; AT5G48030.
GeneIDi834854.
GrameneiAT5G48030.1; AT5G48030.1; AT5G48030.
KEGGiath:AT5G48030.

Similar proteinsi

Entry informationi

Entry nameiGFA2_ARATH
AccessioniPrimary (citable) accession number: Q8GWW8
Secondary accession number(s): Q8L8A1, Q8LEU4, Q9FI35
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 16, 2016
Last sequence update: March 1, 2003
Last modified: October 25, 2017
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families