Q8GWT4 (ANM15_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein arginine N-methyltransferase 1.5
Short name=AtPMRT15
Short name=AtPMRT5
EC=2.1.1.-
EC=2.1.1.125

Alternative name(s):
Shk1 kinase-binding protein 1 homolog

Gene names

Name:	PMRT15
Synonyms:	PMRT5, SKB1
Ordered Locus Names:	At4g31120
ORF Names:	F6E21.40

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	642 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Methylates arginine residues of myelin basic protein (MBP) in vitro. Methylates symmetrically histone H4 of the FLC chromatin to form H4R3me2s, which in turn suppresses FLC expression to induce flowering. Ref.5
Catalytic activity	S-adenosyl-L-methionine + arginine-[histone] = S-adenosyl-L-homocysteine + N(omega)-methyl-arginine-[histone].
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Highly expressed in flowers, roots and siliques, lower expression in stems and mature leaves (at protein level). Abundant in shoot apex, young leaves and leaf primordia, floral and inflorescence meristems, gynoecium, stamens, sepals and young siliques, but not in older leaves, petals and vascular tissues. Ref.5
Developmental stage	High levels from 5 to 20 days after germination (at protein level). Ref.5
Disruption phenotype	Plants are late-flowering. Ref.5
Sequence similarities	Belongs to the protein arginine N-methyltransferase family.
Sequence caution	The sequence CAB79830.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	positive regulation of vernalization response Inferred from mutant phenotype. Source: TAIR regulation of flower development Inferred from mutant phenotype Ref.5. Source: TAIR regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: TAIR
Molecular function	histone-arginine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8GWT4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: Q8GWT4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     492-510: KAHKDLAHFETAYVVKLHS → RLIKILRTLKLLMLSSCIV
     511-642: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8GWT4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     585-642: Missing.

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 642	642	Protein arginine N-methyltransferase 1.5		PRO_0000293993
Natural variations
	Alternative sequence	492 – 510	19	KAHKD…VKLHS → RLIKILRTLKLLMLSSCIV in isoform 2.		VSP_027572
	Alternative sequence	511 – 642	132	Missing in isoform 2.		VSP_027573
	Alternative sequence	585 – 642	58	Missing in isoform 3.		VSP_027574
Experimental info
	Sequence conflict	10	1	E → G in AAO64842. Ref.3
	Sequence conflict	10	1	E → G in BAC43244. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: F9F85054688D1B01	FASTA	642	71,870
	10 20 30 40 50 60 MPLGERGGWE RTESRYCGVE TDFSNDVTHL LNFNISTGGF DYVLAPLVDP SYRPSLVEGN 70 80 90 100 110 120 GVDTQVLPVC GSDLVLSPSQ WSSHVVGKIS SWIDLDSEDE VLRMDSETTL KQEIAWATHL 130 140 150 160 170 180 SLQACLLPTP KGKSCANYAR CVNQILQGLT TLQLWLRVPL VKSEGDSMDD TSEGLNDSWE 190 200 210 220 230 240 LWNSFRLLCE HDSKLSVALD VLSTLPSETS LGRWMGESVR AAILSTDAFL TNARGYPCLS 250 260 270 280 290 300 KRHQKLIAGF FDHAAQVVIC GKPVHNLQKP LDSSSEGTEK NPLRIYLDYV AYLFQKMESL 310 320 330 340 350 360 SEQERIELGY RDFLQAPLQP LMDNLEAQTY ETFERDSVKY IQYQRAVEKA LVDRVPDEKA 370 380 390 400 410 420 SELTTVLMVV GAGRGPLVRA SLQAAEETDR KLKVYAVEKN PNAVVTLHNL VKMEGWEDVV 430 440 450 460 470 480 TIISCDMRFW NAPEQADILV SELLGSFGDN ELSPECLDGA QRFLKPDGIS IPSSYTSFIQ 490 500 510 520 530 540 PITASKLYND VKAHKDLAHF ETAYVVKLHS VAKLAPSQSV FTFTHPNFST KVNNQRYKKL 550 560 570 580 590 600 QFSLPSDAGS ALVHGFAGYF DSVLYKDVHL GIEPTTATPN MFSWFPIFFP LRKPVEVHPD 610 620 630 640 TPLEVHFWRC CGSSKVWYEW SVSSPTPSPM HNTNGRSYWV GL
	Isoform 2 [UniParc]. Checksum: EBB12B9DEC5E838B	FASTA	510	56,926
	10 20 30 40 50 60 MPLGERGGWE RTESRYCGVE TDFSNDVTHL LNFNISTGGF DYVLAPLVDP SYRPSLVEGN 70 80 90 100 110 120 GVDTQVLPVC GSDLVLSPSQ WSSHVVGKIS SWIDLDSEDE VLRMDSETTL KQEIAWATHL 130 140 150 160 170 180 SLQACLLPTP KGKSCANYAR CVNQILQGLT TLQLWLRVPL VKSEGDSMDD TSEGLNDSWE 190 200 210 220 230 240 LWNSFRLLCE HDSKLSVALD VLSTLPSETS LGRWMGESVR AAILSTDAFL TNARGYPCLS 250 260 270 280 290 300 KRHQKLIAGF FDHAAQVVIC GKPVHNLQKP LDSSSEGTEK NPLRIYLDYV AYLFQKMESL 310 320 330 340 350 360 SEQERIELGY RDFLQAPLQP LMDNLEAQTY ETFERDSVKY IQYQRAVEKA LVDRVPDEKA 370 380 390 400 410 420 SELTTVLMVV GAGRGPLVRA SLQAAEETDR KLKVYAVEKN PNAVVTLHNL VKMEGWEDVV 430 440 450 460 470 480 TIISCDMRFW NAPEQADILV SELLGSFGDN ELSPECLDGA QRFLKPDGIS IPSSYTSFIQ 490 500 510 PITASKLYND VRLIKILRTL KLLMLSSCIV
	Isoform 3 [UniParc]. Checksum: CEC3D1D3A3B458B4	FASTA	584	65,098
	10 20 30 40 50 60 MPLGERGGWE RTESRYCGVE TDFSNDVTHL LNFNISTGGF DYVLAPLVDP SYRPSLVEGN 70 80 90 100 110 120 GVDTQVLPVC GSDLVLSPSQ WSSHVVGKIS SWIDLDSEDE VLRMDSETTL KQEIAWATHL 130 140 150 160 170 180 SLQACLLPTP KGKSCANYAR CVNQILQGLT TLQLWLRVPL VKSEGDSMDD TSEGLNDSWE 190 200 210 220 230 240 LWNSFRLLCE HDSKLSVALD VLSTLPSETS LGRWMGESVR AAILSTDAFL TNARGYPCLS 250 260 270 280 290 300 KRHQKLIAGF FDHAAQVVIC GKPVHNLQKP LDSSSEGTEK NPLRIYLDYV AYLFQKMESL 310 320 330 340 350 360 SEQERIELGY RDFLQAPLQP LMDNLEAQTY ETFERDSVKY IQYQRAVEKA LVDRVPDEKA 370 380 390 400 410 420 SELTTVLMVV GAGRGPLVRA SLQAAEETDR KLKVYAVEKN PNAVVTLHNL VKMEGWEDVV 430 440 450 460 470 480 TIISCDMRFW NAPEQADILV SELLGSFGDN ELSPECLDGA QRFLKPDGIS IPSSYTSFIQ 490 500 510 520 530 540 PITASKLYND VKAHKDLAHF ETAYVVKLHS VAKLAPSQSV FTFTHPNFST KVNNQRYKKL 550 560 570 580 QFSLPSDAGS ALVHGFAGYF DSVLYKDVHL GIEPTTATPN MFSW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[4]	"Protein arginine methyltransferases: evolution and assessment of their pharmacological and therapeutic potential." Krause C.D., Yang Z.-H., Kim Y.-S., Lee J.-H., Cook J.R., Pestka S. Pharmacol. Ther. 113:50-87(2007) [PubMed: 17005254] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[5]	"SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis." Wang X., Zhang Y., Ma Q., Zhang Z., Xue Y., Bao S., Chong K. EMBO J. 26:1934-1941(2007) [PubMed: 17363895] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE. Strain: cv. Columbia.
+	Additional computationally mapped references.

Web resources

The Chromatin Database (ChromDB)

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL161578 Genomic DNA. Translation: CAB79830.1. Sequence problems. CP002687 Genomic DNA. Translation: AEE85860.1. CP002687 Genomic DNA. Translation: AEE85861.1. BT005907 mRNA. Translation: AAO64842.1. AK118648 mRNA. Translation: BAC43244.1.
IPI	IPI00544086. IPI00548864. IPI00854313.
PIR	T10666.
RefSeq	NP_194841.2. NM_119262.5. NP_974647.1. NM_202918.2.
UniGene	At.31771. At.44014.
3D structure databases
ProteinModelPortal	Q8GWT4.
SMR	Q8GWT4. Positions 335-639.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8GWT4.
Proteomic databases
PRIDE	Q8GWT4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G31120.1; AT4G31120.1; AT4G31120.
GeneID	829240.
GenomeReviews	Gene locus AT4G31120 in contig CT486007_GR.
KEGG	ath:AT4G31120.
NMPDR	fig|3702.1.peg.21109.
Organism-specific databases
TAIR	At4g31120.
Phylogenomic databases
eggNOG	KOG0822.
GeneTree	EPGT00050000018246.
HOGENOM	HBG628479.
InParanoid	Q8GWT4.
OMA	LCDYNKR.
ProtClustDB	CLSN2680196.
Gene expression databases
Genevestigator	Q8GWT4.
Family and domain databases
InterPro	IPR007857. Skb1_MeTrfase. [Graphical view]
KO	K02516.
PANTHER	PTHR10738. Skb1_mtfrase. 1 hit.
Pfam	PF05185. PRMT5. 1 hit. [Graphical view]
PIRSF	PIRSF015894. Skb1_MeTrfase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ANM15_ARATH

Accession

Primary (citable) accession number: Q8GWT4
Secondary accession number(s): Q9M090

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	November 16, 2011
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program