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Protein

Protein arginine N-methyltransferase 1.5

Gene

PMRT15

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Methylates arginine residues of myelin basic protein (MBP) in vitro. Methylates symmetrically histone H4 of the FLC chromatin to form H4R3me2s, which in turn suppresses FLC expression to induce flowering. Regulates alternative splicing by methylating spliceosomal proteins. Involved in the post-transcriptional regulation of the circadian clock.2 Publications

Catalytic activityi

2 S-adenosyl-L-methionine + [protein]-L-arginine = 2 S-adenosyl-L-homocysteine + [protein]-N(omega),N(omega)-dimethyl-L-arginine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei310 – 3101Peptide substrateBy similarity
Binding sitei330 – 3301S-adenosyl-L-methionineBy similarity
Binding sitei398 – 3981S-adenosyl-L-methionineBy similarity
Active sitei442 – 4421Proton donor/acceptorBy similarity
Active sitei451 – 4511Proton donor/acceptorBy similarity

GO - Molecular functioni

GO - Biological processi

  • peptidyl-arginine methylation, to symmetrical-dimethyl arginine Source: GO_Central
  • positive regulation of vernalization response Source: TAIR
  • regulation of flower development Source: TAIR
  • regulation of transcription, DNA-templated Source: GO_Central
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Methyltransferase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciARA:AT4G31120-MONOMER.
ARA:GQT-1359-MONOMER.
BRENDAi2.1.1.125. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein arginine N-methyltransferase 1.5 (EC:2.1.1.319)
Short name:
AtPMRT15
Short name:
AtPMRT5
Alternative name(s):
Shk1 kinase-binding protein 1 homolog
Gene namesi
Name:PMRT15
Synonyms:PMRT5, SKB1
Ordered Locus Names:At4g31120
ORF Names:F6E21.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G31120.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • nucleus Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Plants are late-flowering. Increased alternative splicing of several genes, including APRR9.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 642642Protein arginine N-methyltransferase 1.5PRO_0000293993Add
BLAST

Proteomic databases

PaxDbiQ8GWT4.
PRIDEiQ8GWT4.

Expressioni

Tissue specificityi

Highly expressed in flowers, roots and siliques, lower expression in stems and mature leaves (at protein level). Abundant in shoot apex, young leaves and leaf primordia, floral and inflorescence meristems, gynoecium, stamens, sepals and young siliques, but not in older leaves, petals and vascular tissues.1 Publication

Developmental stagei

High levels from 5 to 20 days after germination (at protein level).1 Publication

Inductioni

Circadian-regulation.1 Publication

Gene expression databases

GenevisibleiQ8GWT4. AT.

Interactioni

Protein-protein interaction databases

BioGridi14527. 2 interactions.
STRINGi3702.AT4G31120.1.

Structurei

3D structure databases

ProteinModelPortaliQ8GWT4.
SMRiQ8GWT4. Positions 35-642.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini314 – 620307SAM-dependent MTase PRMT-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni339 – 3402S-adenosyl-L-methionine bindingBy similarity
Regioni426 – 4272S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase PRMT-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0822. Eukaryota.
ENOG410XNZM. LUCA.
HOGENOMiHOG000175933.
InParanoidiQ8GWT4.
KOiK02516.
OMAiELEVSMW.
OrthoDBiEOG0936051Q.
PhylomeDBiQ8GWT4.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025799. Arg_MeTrfase.
IPR007857. Arg_MeTrfase_PRMT5.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10738. PTHR10738. 1 hit.
PfamiPF05185. PRMT5. 1 hit.
[Graphical view]
PIRSFiPIRSF015894. Skb1_MeTrfase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51678. SAM_MT_PRMT. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8GWT4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPLGERGGWE RTESRYCGVE TDFSNDVTHL LNFNISTGGF DYVLAPLVDP
60 70 80 90 100
SYRPSLVEGN GVDTQVLPVC GSDLVLSPSQ WSSHVVGKIS SWIDLDSEDE
110 120 130 140 150
VLRMDSETTL KQEIAWATHL SLQACLLPTP KGKSCANYAR CVNQILQGLT
160 170 180 190 200
TLQLWLRVPL VKSEGDSMDD TSEGLNDSWE LWNSFRLLCE HDSKLSVALD
210 220 230 240 250
VLSTLPSETS LGRWMGESVR AAILSTDAFL TNARGYPCLS KRHQKLIAGF
260 270 280 290 300
FDHAAQVVIC GKPVHNLQKP LDSSSEGTEK NPLRIYLDYV AYLFQKMESL
310 320 330 340 350
SEQERIELGY RDFLQAPLQP LMDNLEAQTY ETFERDSVKY IQYQRAVEKA
360 370 380 390 400
LVDRVPDEKA SELTTVLMVV GAGRGPLVRA SLQAAEETDR KLKVYAVEKN
410 420 430 440 450
PNAVVTLHNL VKMEGWEDVV TIISCDMRFW NAPEQADILV SELLGSFGDN
460 470 480 490 500
ELSPECLDGA QRFLKPDGIS IPSSYTSFIQ PITASKLYND VKAHKDLAHF
510 520 530 540 550
ETAYVVKLHS VAKLAPSQSV FTFTHPNFST KVNNQRYKKL QFSLPSDAGS
560 570 580 590 600
ALVHGFAGYF DSVLYKDVHL GIEPTTATPN MFSWFPIFFP LRKPVEVHPD
610 620 630 640
TPLEVHFWRC CGSSKVWYEW SVSSPTPSPM HNTNGRSYWV GL
Note: No experimental confirmation available.
Length:642
Mass (Da):71,870
Last modified:July 10, 2007 - v2
Checksum:iF9F85054688D1B01
GO
Isoform 2 (identifier: Q8GWT4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     492-510: KAHKDLAHFETAYVVKLHS → RLIKILRTLKLLMLSSCIV
     511-642: Missing.

Note: No experimental confirmation available.
Show »
Length:510
Mass (Da):56,926
Checksum:iEBB12B9DEC5E838B
GO
Isoform 3 (identifier: Q8GWT4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     585-642: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:584
Mass (Da):65,098
Checksum:iCEC3D1D3A3B458B4
GO

Sequence cautioni

The sequence CAB79830 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 101E → G in AAO64842 (PubMed:14593172).Curated
Sequence conflicti10 – 101E → G in BAC43244 (PubMed:14593172).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei492 – 51019KAHKD…VKLHS → RLIKILRTLKLLMLSSCIV in isoform 2. 1 PublicationVSP_027572Add
BLAST
Alternative sequencei511 – 642132Missing in isoform 2. 1 PublicationVSP_027573Add
BLAST
Alternative sequencei585 – 64258Missing in isoform 3. CuratedVSP_027574Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL161578 Genomic DNA. Translation: CAB79830.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE85860.1.
CP002687 Genomic DNA. Translation: AEE85861.1.
BT005907 mRNA. Translation: AAO64842.1.
AK118648 mRNA. Translation: BAC43244.1.
PIRiT10666.
RefSeqiNP_194841.2. NM_119262.5. [Q8GWT4-1]
NP_974647.1. NM_202918.2. [Q8GWT4-3]
UniGeneiAt.31771.
At.44014.

Genome annotation databases

EnsemblPlantsiAT4G31120.1; AT4G31120.1; AT4G31120. [Q8GWT4-1]
GeneIDi829240.
KEGGiath:AT4G31120.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL161578 Genomic DNA. Translation: CAB79830.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE85860.1.
CP002687 Genomic DNA. Translation: AEE85861.1.
BT005907 mRNA. Translation: AAO64842.1.
AK118648 mRNA. Translation: BAC43244.1.
PIRiT10666.
RefSeqiNP_194841.2. NM_119262.5. [Q8GWT4-1]
NP_974647.1. NM_202918.2. [Q8GWT4-3]
UniGeneiAt.31771.
At.44014.

3D structure databases

ProteinModelPortaliQ8GWT4.
SMRiQ8GWT4. Positions 35-642.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14527. 2 interactions.
STRINGi3702.AT4G31120.1.

Proteomic databases

PaxDbiQ8GWT4.
PRIDEiQ8GWT4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G31120.1; AT4G31120.1; AT4G31120. [Q8GWT4-1]
GeneIDi829240.
KEGGiath:AT4G31120.

Organism-specific databases

TAIRiAT4G31120.

Phylogenomic databases

eggNOGiKOG0822. Eukaryota.
ENOG410XNZM. LUCA.
HOGENOMiHOG000175933.
InParanoidiQ8GWT4.
KOiK02516.
OMAiELEVSMW.
OrthoDBiEOG0936051Q.
PhylomeDBiQ8GWT4.

Enzyme and pathway databases

BioCyciARA:AT4G31120-MONOMER.
ARA:GQT-1359-MONOMER.
BRENDAi2.1.1.125. 399.

Miscellaneous databases

PROiQ8GWT4.

Gene expression databases

GenevisibleiQ8GWT4. AT.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025799. Arg_MeTrfase.
IPR007857. Arg_MeTrfase_PRMT5.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10738. PTHR10738. 1 hit.
PfamiPF05185. PRMT5. 1 hit.
[Graphical view]
PIRSFiPIRSF015894. Skb1_MeTrfase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51678. SAM_MT_PRMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANM15_ARATH
AccessioniPrimary (citable) accession number: Q8GWT4
Secondary accession number(s): Q9M090
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: September 7, 2016
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.