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Protein

Glutaredoxin-C5, chloroplastic

Gene

GRXC5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins. Can assemble a [2Fe-2S] cluster, but cannot transfer it to an apoferredoxin.1 Publication

Kineticsi

kcat is 1.21 sec(-1) with glutathionylated beta-mercaptoethanol as substrate. kcat is 0.23 sec(-1) with dehydroascorbate as substrate. kcat is 0.69 sec(-1) with reduced glutathione as substrate.1 Publication
  1. KM=0.20 mM for glutathionylated beta-mercaptoethanol1 Publication
  2. KM=0.21 mM for dehydroascorbate1 Publication
  3. KM=3.6 mM for reduced glutathione1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei135Glutathione; via amide nitrogen and carbonyl oxygen1 Publication1
    Binding sitei148Glutathione; via amide nitrogen1 Publication1
    Binding sitei149Glutathione; via amide nitrogen1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    • cell redox homeostasis Source: InterPro
    • iron-sulfur cluster assembly Source: TAIR

    Keywordsi

    Biological processElectron transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutaredoxin-C5, chloroplastic1 Publication
    Short name:
    AtGrxC51 Publication
    Gene namesi
    Name:GRXC51 Publication
    Ordered Locus Names:At4g28730Imported
    ORF Names:F16A16.160Imported
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 4

    Organism-specific databases

    AraportiAT4G28730
    TAIRilocus:2117793 AT4G28730

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Chloroplast, Plastid

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi90C → S: Loss of Fe-S cluster incorporation and loss of glutaredoxin activity. 1 Publication1
    Mutagenesisi93C → S: Loss of Fe-S cluster incorporation, but increased glutaredoxin activity. 1 Publication1
    Mutagenesisi141C → S: No effect on Fe-S cluster incorporation or on glutaredoxin activity. 1 Publication1
    Mutagenesisi148C → S: No effect on Fe-S cluster incorporation or on glutaredoxin activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 51ChloroplastCombined sourcesCuratedAdd BLAST51
    ChainiPRO_000026871252 – 174Glutaredoxin-C5, chloroplasticAdd BLAST123

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei52N-acetylthreonineCombined sources1
    Disulfide bondi90 ↔ 93Redox-activeBy similarity
    Modified residuei90S-glutathionyl cysteine; partial1 Publication1
    Modified residuei148S-glutathionyl cysteine; partial1 Publication1

    Post-translational modificationi

    Glutathionylated.1 Publication

    Keywords - PTMi

    Acetylation, Disulfide bond, Glutathionylation

    Proteomic databases

    PaxDbiQ8GWS0

    Expressioni

    Inductioni

    Up-regulated by cold treatment.1 Publication

    Gene expression databases

    ExpressionAtlasiQ8GWS0 baseline and differential

    Interactioni

    Subunit structurei

    Monomeric apoprotein and homodimeric holoprotein containing a [2Fe-2S] cluster (PubMed:21632542). No in vitro interactions with SUFE1, BOLA1, BOLA2 or BOLA4 (PubMed:24203231).2 Publications

    Protein-protein interaction databases

    BioGridi14281, 5 interactors
    IntActiQ8GWS0, 2 interactors
    STRINGi3702.AT4G28730.1

    Structurei

    Secondary structure

    1174
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi68 – 79Combined sources12
    Beta strandi80 – 86Combined sources7
    Helixi91 – 102Combined sources12
    Beta strandi108 – 111Combined sources4
    Helixi112 – 114Combined sources3
    Helixi118 – 130Combined sources13
    Beta strandi137 – 140Combined sources4
    Beta strandi143 – 147Combined sources5
    Helixi148 – 156Combined sources9
    Helixi159 – 164Combined sources6

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3RHBX-ray1.20A64-174[»]
    3RHCX-ray2.40A/B64-174[»]
    ProteinModelPortaliQ8GWS0
    SMRiQ8GWS0
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8GWS0

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini93 – 171GlutaredoxinPROSITE-ProRule annotationAdd BLAST79

    Sequence similaritiesi

    Belongs to the glutaredoxin family. CPYC subfamily.Curated

    Keywords - Domaini

    Redox-active center, Transit peptide

    Phylogenomic databases

    eggNOGiKOG1752 Eukaryota
    COG0695 LUCA
    HOGENOMiHOG000095204
    InParanoidiQ8GWS0
    OMAiARMEDSV
    OrthoDBiEOG09360R46
    PhylomeDBiQ8GWS0

    Family and domain databases

    InterProiView protein in InterPro
    IPR002109 Glutaredoxin
    IPR011899 Glutaredoxin_euk/vir
    IPR014025 Glutaredoxin_subgr
    IPR036249 Thioredoxin-like_sf
    PfamiView protein in Pfam
    PF00462 Glutaredoxin, 1 hit
    PRINTSiPR00160 GLUTAREDOXIN
    SUPFAMiSSF52833 SSF52833, 1 hit
    TIGRFAMsiTIGR02180 GRX_euk, 1 hit
    PROSITEiView protein in PROSITE
    PS51354 GLUTAREDOXIN_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8GWS0-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAVTAFNTLK LVSSSLDPIP SVSCSSYSFS LIYVGSPYKR CLKQSCSVRA
    60 70 80 90 100
    MTSSSSAASS SSSSFGSRME ESIRKTVTEN TVVIYSKTWC SYCTEVKTLF
    110 120 130 140 150
    KRLGVQPLVV ELDQLGPQGP QLQKVLERLT GQHTVPNVFV CGKHIGGCTD
    160 170
    TVKLNRKGDL ELMLAEANGK NGQS
    Length:174
    Mass (Da):18,814
    Last modified:March 1, 2003 - v1
    Checksum:iD7912FC4FBB01743
    GO

    Sequence cautioni

    The sequence CAA22979 differs from that shown. Reason: Erroneous gene model prediction.Curated
    The sequence CAB81461 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL035353 Genomic DNA Translation: CAA22979.1 Sequence problems.
    AL161573 Genomic DNA Translation: CAB81461.1 Sequence problems.
    CP002687 Genomic DNA Translation: AEE85535.1
    AK118672 mRNA Translation: BAC43267.1
    BT003703 mRNA Translation: AAO39931.1
    PIRiT04526
    RefSeqiNP_194602.2, NM_119017.5
    UniGeneiAt.31993

    Genome annotation databases

    EnsemblPlantsiAT4G28730.1; AT4G28730.1; AT4G28730
    GeneIDi828994
    GrameneiAT4G28730.1; AT4G28730.1; AT4G28730
    KEGGiath:AT4G28730

    Similar proteinsi

    Entry informationi

    Entry nameiGRXC5_ARATH
    AccessioniPrimary (citable) accession number: Q8GWS0
    Secondary accession number(s): Q9SVU1
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
    Last sequence update: March 1, 2003
    Last modified: April 25, 2018
    This is version 100 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health