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Protein

Inositol phosphorylceramide glucuronosyltransferase 1

Gene

IPUT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates the transfer of glucuronic acid (GlcA) from UDP-GlcA to glycosyl inositol phosphorylceramides (GIPCs). The formation of GIPCs sphingolipids seems essential for pollen function.1 Publication

Catalytic activityi

UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei108By similarity1
Metal bindingi124ManganeseBy similarity1
Metal bindingi126ManganeseBy similarity1
Sitei183Important for catalytic activityBy similarity1
Metal bindingi248ManganeseBy similarity1

GO - Molecular functioni

  • glucuronosyltransferase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • sphingolipid alpha-glucuronosyltransferase activity Source: TAIR

GO - Biological processi

  • ceramide biosynthetic process Source: TAIR

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandManganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G18480-MONOMER
ReactomeiR-ATH-6798695 Neutrophil degranulation
R-ATH-70221 Glycogen breakdown (glycogenolysis)

Protein family/group databases

CAZyiGT8 Glycosyltransferase Family 8

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol phosphorylceramide glucuronosyltransferase 11 Publication (EC:2.4.1.171 Publication)
Alternative name(s):
Glycogenin-like protein 6
Plant glycogenin-like starch initiation protein 6
Gene namesi
Name:IPUT11 Publication
Synonyms:PGSIP6
Ordered Locus Names:At5g18480Imported
ORF Names:T28N17.3Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G18480
TAIRilocus:2146173 AT5G18480

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei6 – 26HelicalSequence analysisAdd BLAST21
Transmembranei293 – 313HelicalSequence analysisAdd BLAST21
Transmembranei375 – 395HelicalSequence analysisAdd BLAST21
Transmembranei406 – 426HelicalSequence analysisAdd BLAST21
Transmembranei468 – 488HelicalSequence analysisAdd BLAST21
Transmembranei494 – 514HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Defect in transmission of mutated alleles through the male gametophyte (e.g. pollen).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004167381 – 537Inositol phosphorylceramide glucuronosyltransferase 1Add BLAST537

Proteomic databases

PaxDbiQ8GWB7
PRIDEiQ8GWB7

PTM databases

SwissPalmiQ8GWB7

Expressioni

Tissue specificityi

Expressed in seedlings, roots, leaves, stems and siliques.1 Publication

Developmental stagei

Expressed throughout pollen development.1 Publication

Gene expression databases

ExpressionAtlasiQ8GWB7 baseline and differential

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G18480.1

Structurei

3D structure databases

ProteinModelPortaliQ8GWB7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1950 Eukaryota
COG5597 LUCA
HOGENOMiHOG000006378
InParanoidiQ8GWB7
OMAiYQSCFQT
OrthoDBiEOG093607D8
PhylomeDBiQ8GWB7

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR002495 Glyco_trans_8
IPR029044 Nucleotide-diphossugar_trans
PfamiView protein in Pfam
PF01501 Glyco_transf_8, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

Q8GWB7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRLKTSLWV LLLALVSIQL NGSFGSESSK VAYVTLLYGD EFLLGVRVLG
60 70 80 90 100
KSIRDTGSTK DMVALVSDGV SDYSKKLLKA DGWKVEKISL LANPNQVHPT
110 120 130 140 150
RFWGVYTKLK IFNMTDYKKV VYLDADTIVV KNIEDLFKCS KFCANLKHSE
160 170 180 190 200
RLNSGVMVVE PSEALFNDMM RKVKTLSSYT GGDQGFLNSY YPDFPNARVF
210 220 230 240 250
DPSVTPEVLK TRPVPAMERL STLYNADVGL YMLANKWMVD DSKLHVIHYT
260 270 280 290 300
LGPLKPWDWW TAWLVKPVDA WHSIRVKLEE TLPGTGGGSN QHDELVVKFL
310 320 330 340 350
FLLPLCALLF CIYRSIQGRE GSLCWSSFSN QIRYLYYKVR SNGTLGYGGV
360 370 380 390 400
STMSPSYQPH SGNAQSKVPQ HLGAVSVVVC FTAVLLSLGI SFAIVPRQIM
410 420 430 440 450
PWTGLVLVYE WTFTIFFLLF GVFLLFVHQH GKRIAIQSES SSLDDSAKVH
460 470 480 490 500
QRAGGSCDVT TLYYGLGMAF LAIAAVSLPY ILGITALFTR LGLMVGLAII
510 520 530
LAAFMTYASE HLAVRWFLKG LEDRRDTTRS NSLCFLC
Length:537
Mass (Da):60,011
Last modified:March 1, 2003 - v1
Checksum:iAA561C73A1064E2F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti182G → R in AAL58891 (PubMed:14593172).Curated1
Sequence conflicti182G → R in AAN28860 (PubMed:14593172).Curated1
Sequence conflicti389G → E in AAL58891 (PubMed:14593172).Curated1
Sequence conflicti389G → E in AAN28860 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KJ138680 mRNA Translation: AHL38620.1
AC051626 Genomic DNA No translation available.
CP002688 Genomic DNA Translation: AED92569.1
AK118951 mRNA Translation: BAC43531.1
AF462795 mRNA Translation: AAL58891.1
AY143921 mRNA Translation: AAN28860.1
RefSeqiNP_197349.2, NM_121853.4
UniGeneiAt.31418

Genome annotation databases

EnsemblPlantsiAT5G18480.1; AT5G18480.1; AT5G18480
GeneIDi831966
GrameneiAT5G18480.1; AT5G18480.1; AT5G18480
KEGGiath:AT5G18480

Similar proteinsi

Entry informationi

Entry nameiGUX6_ARATH
AccessioniPrimary (citable) accession number: Q8GWB7
Secondary accession number(s): Q8W118, W8Q2T0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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