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Protein

Inositol phosphorylceramide glucuronosyltransferase 1

Gene

IPUT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the transfer of glucuronic acid (GlcA) from UDP-GlcA to glycosyl inositol phosphorylceramides (GIPCs). The formation of GIPCs sphingolipids seems essential for pollen function.1 Publication

Catalytic activityi

UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei108 – 1081By similarity
Metal bindingi124 – 1241ManganeseBy similarity
Metal bindingi126 – 1261ManganeseBy similarity
Sitei183 – 1831Important for catalytic activityBy similarity
Metal bindingi248 – 2481ManganeseBy similarity

GO - Molecular functioni

  • glucuronosyltransferase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • sphingolipid alpha-glucuronosyltransferase activity Source: TAIR

GO - Biological processi

  • ceramide biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G18480-MONOMER.
ReactomeiR-ATH-5357572. Lysosomal glycogen catabolism.
R-ATH-70221. Glycogen breakdown (glycogenolysis).

Protein family/group databases

CAZyiGT8. Glycosyltransferase Family 8.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol phosphorylceramide glucuronosyltransferase 11 Publication (EC:2.4.1.171 Publication)
Alternative name(s):
Glycogenin-like protein 6
Plant glycogenin-like starch initiation protein 6
Gene namesi
Name:IPUT11 Publication
Synonyms:PGSIP6
Ordered Locus Names:At5g18480Imported
ORF Names:T28N17.3Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G18480.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei6 – 2621HelicalSequence analysisAdd
BLAST
Transmembranei293 – 31321HelicalSequence analysisAdd
BLAST
Transmembranei375 – 39521HelicalSequence analysisAdd
BLAST
Transmembranei406 – 42621HelicalSequence analysisAdd
BLAST
Transmembranei468 – 48821HelicalSequence analysisAdd
BLAST
Transmembranei494 – 51421HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • endosome Source: TAIR
  • Golgi apparatus Source: TAIR
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: TAIR
  • trans-Golgi network Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Defect in transmission of mutated alleles through the male gametophyte (e.g. pollen).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 537537Inositol phosphorylceramide glucuronosyltransferase 1PRO_0000416738Add
BLAST

Proteomic databases

PaxDbiQ8GWB7.
PRIDEiQ8GWB7.

PTM databases

SwissPalmiQ8GWB7.

Expressioni

Tissue specificityi

Expressed in seedlings, roots, leaves, stems and siliques.1 Publication

Developmental stagei

Expressed throughout pollen development.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G18480.1.

Structurei

3D structure databases

ProteinModelPortaliQ8GWB7.
SMRiQ8GWB7. Positions 32-259.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1950. Eukaryota.
COG5597. LUCA.
HOGENOMiHOG000006378.
InParanoidiQ8GWB7.
OMAiSAFYKGQ.
PhylomeDBiQ8GWB7.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR002495. Glyco_trans_8.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01501. Glyco_transf_8. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8GWB7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRLKTSLWV LLLALVSIQL NGSFGSESSK VAYVTLLYGD EFLLGVRVLG
60 70 80 90 100
KSIRDTGSTK DMVALVSDGV SDYSKKLLKA DGWKVEKISL LANPNQVHPT
110 120 130 140 150
RFWGVYTKLK IFNMTDYKKV VYLDADTIVV KNIEDLFKCS KFCANLKHSE
160 170 180 190 200
RLNSGVMVVE PSEALFNDMM RKVKTLSSYT GGDQGFLNSY YPDFPNARVF
210 220 230 240 250
DPSVTPEVLK TRPVPAMERL STLYNADVGL YMLANKWMVD DSKLHVIHYT
260 270 280 290 300
LGPLKPWDWW TAWLVKPVDA WHSIRVKLEE TLPGTGGGSN QHDELVVKFL
310 320 330 340 350
FLLPLCALLF CIYRSIQGRE GSLCWSSFSN QIRYLYYKVR SNGTLGYGGV
360 370 380 390 400
STMSPSYQPH SGNAQSKVPQ HLGAVSVVVC FTAVLLSLGI SFAIVPRQIM
410 420 430 440 450
PWTGLVLVYE WTFTIFFLLF GVFLLFVHQH GKRIAIQSES SSLDDSAKVH
460 470 480 490 500
QRAGGSCDVT TLYYGLGMAF LAIAAVSLPY ILGITALFTR LGLMVGLAII
510 520 530
LAAFMTYASE HLAVRWFLKG LEDRRDTTRS NSLCFLC
Length:537
Mass (Da):60,011
Last modified:March 1, 2003 - v1
Checksum:iAA561C73A1064E2F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti182 – 1821G → R in AAL58891 (PubMed:14593172).Curated
Sequence conflicti182 – 1821G → R in AAN28860 (PubMed:14593172).Curated
Sequence conflicti389 – 3891G → E in AAL58891 (PubMed:14593172).Curated
Sequence conflicti389 – 3891G → E in AAN28860 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KJ138680 mRNA. Translation: AHL38620.1.
AC051626 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92569.1.
AK118951 mRNA. Translation: BAC43531.1.
AF462795 mRNA. Translation: AAL58891.1.
AY143921 mRNA. Translation: AAN28860.1.
RefSeqiNP_197349.2. NM_121853.3.
UniGeneiAt.31418.

Genome annotation databases

EnsemblPlantsiAT5G18480.1; AT5G18480.1; AT5G18480.
GeneIDi831966.
GrameneiAT5G18480.1; AT5G18480.1; AT5G18480.
KEGGiath:AT5G18480.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KJ138680 mRNA. Translation: AHL38620.1.
AC051626 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92569.1.
AK118951 mRNA. Translation: BAC43531.1.
AF462795 mRNA. Translation: AAL58891.1.
AY143921 mRNA. Translation: AAN28860.1.
RefSeqiNP_197349.2. NM_121853.3.
UniGeneiAt.31418.

3D structure databases

ProteinModelPortaliQ8GWB7.
SMRiQ8GWB7. Positions 32-259.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G18480.1.

Protein family/group databases

CAZyiGT8. Glycosyltransferase Family 8.

PTM databases

SwissPalmiQ8GWB7.

Proteomic databases

PaxDbiQ8GWB7.
PRIDEiQ8GWB7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G18480.1; AT5G18480.1; AT5G18480.
GeneIDi831966.
GrameneiAT5G18480.1; AT5G18480.1; AT5G18480.
KEGGiath:AT5G18480.

Organism-specific databases

TAIRiAT5G18480.

Phylogenomic databases

eggNOGiKOG1950. Eukaryota.
COG5597. LUCA.
HOGENOMiHOG000006378.
InParanoidiQ8GWB7.
OMAiSAFYKGQ.
PhylomeDBiQ8GWB7.

Enzyme and pathway databases

BioCyciARA:AT5G18480-MONOMER.
ReactomeiR-ATH-5357572. Lysosomal glycogen catabolism.
R-ATH-70221. Glycogen breakdown (glycogenolysis).

Miscellaneous databases

PROiQ8GWB7.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR002495. Glyco_trans_8.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01501. Glyco_transf_8. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a sphingolipid alpha-glucuronosyltransferase that is essential for pollen function in Arabidopsis."
    Rennie E.A., Ebert B., Miles G.P., Cahoon R.E., Christiansen K.M., Stonebloom S., Khatab H., Twell D., Petzold C.J., Adams P.D., Dupree P., Heazlewood J.L., Cahoon E.B., Scheller H.V.
    Plant Cell 26:3314-3325(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Reduced expression of a protein homologous to glycogenin leads to reduction of starch content in Arabidopsis leaves."
    Chatterjee M., Berbezy P., Vyas D., Coates S., Barsby T.
    Plant Sci. 168:501-509(2005)
    [AGRICOLA] [Europe PMC]
    Cited for: GENE FAMILY.
  7. "Three members of the Arabidopsis glycosyltransferase family 8 are xylan glucuronosyltransferases."
    Rennie E.A., Hansen S.F., Baidoo E.E.K., Hadi M.Z., Keasling J.D., Scheller H.V.
    Plant Physiol. 159:1408-1417(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiGUX6_ARATH
AccessioniPrimary (citable) accession number: Q8GWB7
Secondary accession number(s): Q8W118, W8Q2T0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: March 1, 2003
Last modified: February 17, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.