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Q8GW72

- FUCO1_ARATH

UniProt

Q8GW72 - FUCO1_ARATH

Protein

Alpha-L-fucosidase 1

Gene

FUC1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 2 (03 May 2011)
      Previous versions | rss
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    Functioni

    Hydrolyzes both 3- and 4-linked fucoses in Lewis determinants. Not active on neither 2-linked fucose nor on fucose in alpha-1,3-linkage to the innermost GlcNAc.1 Publication

    Catalytic activityi

    An alpha-L-fucoside + H2O = L-fucose + an alcohol.

    Kineticsi

    1. KM=28 µM for lacto-N-fucopentaose II1 Publication
    2. KM=6.2 µM for 3-fucosyllactose1 Publication

    pH dependencei

    Optimum pH is 5. Activity decreases sharply toward pH 7.1 Publication

    GO - Molecular functioni

    1. alpha-L-fucosidase activity Source: TAIR

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. glycoprotein catabolic process Source: TAIR

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT2G28100-MONOMER.
    MetaCyc:AT2G28100-MONOMER.

    Protein family/group databases

    CAZyiGH29. Glycoside Hydrolase Family 29.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-L-fucosidase 1 (EC:3.2.1.51)
    Alternative name(s):
    Alpha-1,3/4-fucosidase
    Short name:
    AtFUC1
    Alpha-L-fucoside fucohydrolase
    Gene namesi
    Name:FUC1
    Ordered Locus Names:At2g28100
    ORF Names:F24D13.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G28100.

    Subcellular locationi

    Secretedextracellular spaceapoplast 1 Publication

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell
    2. vacuole Source: TAIR

    Keywords - Cellular componenti

    Apoplast, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 506483Alpha-L-fucosidase 1PRO_0000225692Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi22 – 221N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi82 – 821N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi248 – 2481N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi320 – 3201N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi355 – 3551N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi487 – 4871N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ8GW72.
    PRIDEiQ8GW72.

    Expressioni

    Gene expression databases

    GenevestigatoriQ8GW72.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BRL1Q9ZPS91EBI-2293793,EBI-2292728

    Protein-protein interaction databases

    IntActiQ8GW72. 1 interaction.
    STRINGi3702.AT2G28100.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8GW72.
    SMRiQ8GW72. Positions 38-472.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 29 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3669.
    HOGENOMiHOG000221495.
    InParanoidiQ8GW72.
    KOiK01206.
    OMAiYWIANEN.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR008979. Galactose-bd-like.
    IPR000933. Glyco_hydro_29.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10030. PTHR10030. 1 hit.
    PfamiPF01120. Alpha_L_fucos. 1 hit.
    [Graphical view]
    SMARTiSM00812. Alpha_L_fucos. 1 hit.
    [Graphical view]
    SUPFAMiSSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8GW72-1 [UniParc]FASTAAdd to Basket

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    MNSQITLFFF FFSILSLSQI SNSSSLLKPH PCPILPLPSS QQLQWQLGSM    50
    AMFLHFGPNT FTDSEWGTGK ANPSIFNPTH LNASQWVQIA KDSGFSRVIL 100
    TAKHHDGFCL WPSEYTDYSV KSSQWRNGAG DVVAELASAA KEAGIGLGLY 150
    LSPWDRHEQC YGKTLEYNEF YLSQMTELLT KYGEIKEVWL DGAKGDGEKD 200
    MEYFFDTWFS LIHQLQPKAV IFSDAGPDVR WIGDEAGLAG STCWSLFNRT 250
    NAKIGDTEPS YSQEGDGYGQ DWVPAECDVS IRPGWFWHAS ESPKPAVQLL 300
    DIYYNSVGRN CLFLLNVPPN SSGLISEQDI KVLEEFSEMK NSIFSNNLAR 350
    KAFVNSSSIR GDQSSQFGPK NVLEEGLDKY WAPEENQNEW VLYLEFKDLV 400
    SFNVLEIREP IHMGQRIASF HLETRKTGSG EWERVVSGTT VGNKRLLRFL 450
    NVVESRSLKL VVDKARTDPL ISYLGLYMDK FSGSSRNTTK ITITRTLKEE 500
    QQLHDL 506
    Length:506
    Mass (Da):57,186
    Last modified:May 3, 2011 - v2
    Checksum:i5DD0B508E4560CB9
    GO

    Sequence cautioni

    The sequence AAC98456.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti135 – 1351E → G in BAC43615. (PubMed:11910074)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC005851 Genomic DNA. Translation: AAC98456.1. Sequence problems.
    CP002685 Genomic DNA. Translation: AEC08079.1.
    AK119039 mRNA. Translation: BAC43615.1.
    PIRiG84680.
    RefSeqiNP_180377.2. NM_128370.3.
    UniGeneiAt.38664.
    At.66367.

    Genome annotation databases

    EnsemblPlantsiAT2G28100.1; AT2G28100.1; AT2G28100.
    GeneIDi817355.
    KEGGiath:AT2G28100.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC005851 Genomic DNA. Translation: AAC98456.1 . Sequence problems.
    CP002685 Genomic DNA. Translation: AEC08079.1 .
    AK119039 mRNA. Translation: BAC43615.1 .
    PIRi G84680.
    RefSeqi NP_180377.2. NM_128370.3.
    UniGenei At.38664.
    At.66367.

    3D structure databases

    ProteinModelPortali Q8GW72.
    SMRi Q8GW72. Positions 38-472.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8GW72. 1 interaction.
    STRINGi 3702.AT2G28100.1-P.

    Protein family/group databases

    CAZyi GH29. Glycoside Hydrolase Family 29.

    Proteomic databases

    PaxDbi Q8GW72.
    PRIDEi Q8GW72.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G28100.1 ; AT2G28100.1 ; AT2G28100 .
    GeneIDi 817355.
    KEGGi ath:AT2G28100.

    Organism-specific databases

    TAIRi AT2G28100.

    Phylogenomic databases

    eggNOGi COG3669.
    HOGENOMi HOG000221495.
    InParanoidi Q8GW72.
    KOi K01206.
    OMAi YWIANEN.

    Enzyme and pathway databases

    BioCyci ARA:AT2G28100-MONOMER.
    MetaCyc:AT2G28100-MONOMER.

    Gene expression databases

    Genevestigatori Q8GW72.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR008979. Galactose-bd-like.
    IPR000933. Glyco_hydro_29.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10030. PTHR10030. 1 hit.
    Pfami PF01120. Alpha_L_fucos. 1 hit.
    [Graphical view ]
    SMARTi SM00812. Alpha_L_fucos. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "AtFXG1, an Arabidopsis gene encoding alpha-L-fucosidase active against fucosylated xyloglucan oligosaccharides."
      de La Torre F., Sampedro J., Zarra I., Revilla G.
      Plant Physiol. 128:247-255(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY FUNCTION, SUBCELLULAR LOCATION.
    5. "Molecular cloning and characterization of a plant alpha1,3/4-fucosidase based on sequence tags from almond fucosidase I."
      Zeleny R., Leonard R., Dorfner G., Dalik T., Kolarich D., Altmann F.
      Phytochemistry 67:641-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiFUCO1_ARATH
    AccessioniPrimary (citable) accession number: Q8GW72
    Secondary accession number(s): Q9ZUV2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 7, 2006
    Last sequence update: May 3, 2011
    Last modified: October 1, 2014
    This is version 84 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Caution

    Was reported by PubMed:11788770 to be an alpha-1,2-fucosidase.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3