Q8GW72 (FUCO1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-L-fucosidase 1
EC=3.2.1.51

Alternative name(s):
Alpha-1,3/4-fucosidase
Short name=AtFUC1
Alpha-L-fucoside fucohydrolase

Gene names

Name:	FUC1
Ordered Locus Names:	At2g28100
ORF Names:	F24D13.11

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes both 3- and 4-linked fucoses in Lewis determinants. Not active on neither 2-linked fucose nor on fucose in alpha-1,3-linkage to the innermost GlcNAc. Ref.4 Ref.5
Catalytic activity	An alpha-L-fucoside + H2O = L-fucose + an alcohol.
Subcellular location	Secreted › extracellular space › apoplast Ref.4.
Sequence similarities	Belongs to the glycosyl hydrolase 29 family.
Caution	Was reported by Ref.4 to be an alpha-1,2-fucosidase.
Biophysicochemical properties	Kinetic parameters: KM=28 µM for lacto-N-fucopentaose II Ref.5 KM=6.2 µM for 3-fucosyllactose pH dependence: Optimum pH is 5. Activity decreases sharply toward pH 7.
Sequence caution	The sequence AAC98456.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Apoplast Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro glycoprotein catabolic process Traceable author statement Ref.5. Source: TAIR
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell vacuole Inferred from direct assay. Source: TAIR
Molecular function	alpha-L-fucosidase activity Inferred from direct assay Ref.4Ref.5. Source: TAIR cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BRL1	Q9ZPS9	1	EBI-2293793,EBI-2292728

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 506	483	Alpha-L-fucosidase 1		PRO_0000225692
Amino acid modifications
	Glycosylation	22	1	N-linked (GlcNAc...) Potential
	Glycosylation	82	1	N-linked (GlcNAc...) Potential
	Glycosylation	248	1	N-linked (GlcNAc...) Potential
	Glycosylation	320	1	N-linked (GlcNAc...) Potential
	Glycosylation	355	1	N-linked (GlcNAc...) Potential
	Glycosylation	487	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	135	1	E → G in BAC43615. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8GW72 [UniParc]. Last modified May 3, 2011. Version 2. Checksum: 5DD0B508E4560CB9	FASTA	506	57,186
	10 20 30 40 50 60 MNSQITLFFF FFSILSLSQI SNSSSLLKPH PCPILPLPSS QQLQWQLGSM AMFLHFGPNT 70 80 90 100 110 120 FTDSEWGTGK ANPSIFNPTH LNASQWVQIA KDSGFSRVIL TAKHHDGFCL WPSEYTDYSV 130 140 150 160 170 180 KSSQWRNGAG DVVAELASAA KEAGIGLGLY LSPWDRHEQC YGKTLEYNEF YLSQMTELLT 190 200 210 220 230 240 KYGEIKEVWL DGAKGDGEKD MEYFFDTWFS LIHQLQPKAV IFSDAGPDVR WIGDEAGLAG 250 260 270 280 290 300 STCWSLFNRT NAKIGDTEPS YSQEGDGYGQ DWVPAECDVS IRPGWFWHAS ESPKPAVQLL 310 320 330 340 350 360 DIYYNSVGRN CLFLLNVPPN SSGLISEQDI KVLEEFSEMK NSIFSNNLAR KAFVNSSSIR 370 380 390 400 410 420 GDQSSQFGPK NVLEEGLDKY WAPEENQNEW VLYLEFKDLV SFNVLEIREP IHMGQRIASF 430 440 450 460 470 480 HLETRKTGSG EWERVVSGTT VGNKRLLRFL NVVESRSLKL VVDKARTDPL ISYLGLYMDK 490 500 FSGSSRNTTK ITITRTLKEE QQLHDL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed: 11910074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"AtFXG1, an Arabidopsis gene encoding alpha-L-fucosidase active against fucosylated xyloglucan oligosaccharides." de La Torre F., Sampedro J., Zarra I., Revilla G. Plant Physiol. 128:247-255(2002) [PubMed: 11788770] [Abstract] Cited for: PRELIMINARY FUNCTION, SUBCELLULAR LOCATION.
[5]	"Molecular cloning and characterization of a plant alpha1,3/4-fucosidase based on sequence tags from almond fucosidase I." Zeleny R., Leonard R., Dorfner G., Dalik T., Kolarich D., Altmann F. Phytochemistry 67:641-648(2006) [PubMed: 16516937] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC005851 Genomic DNA. Translation: AAC98456.1. Sequence problems. CP002685 Genomic DNA. Translation: AEC08079.1. AK119039 mRNA. Translation: BAC43615.1.
IPI	IPI00519340.
PIR	G84680.
RefSeq	NP_180377.2. NM_128370.3.
UniGene	At.38664. At.66367.
3D structure databases
ProteinModelPortal	Q8GW72.
SMR	Q8GW72. Positions 32-481.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8GW72. 1 interaction.
STRING	Q8GW72.
Protein family/group databases
CAZy	GH29. Glycoside Hydrolase Family 29.
Proteomic databases
PRIDE	Q8GW72.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G28100.1; AT2G28100.1; AT2G28100.
GeneID	817355.
GenomeReviews	Gene locus AT2G28100 in contig CT485783_GR.
KEGG	ath:AT2G28100.
Organism-specific databases
TAIR	At2g28100.
Phylogenomic databases
eggNOG	KOG3340.
GeneTree	EPGT00050000019384.
HOGENOM	HBG697253.
InParanoid	Q8GW72.
OMA	VKHHDGF.
Enzyme and pathway databases
BioCyc	ARA:AT2G28100-MONOMER. MetaCyc:AT2G28100-MONOMER.
Gene expression databases
Genevestigator	Q8GW72.
GermOnline	AT2G28100. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR008979. Galactose-bd-like. IPR000933. Glyco_hydro_29. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10030. Glyco_hydro_29. 1 hit.
Pfam	PF01120. Alpha_L_fucos. 1 hit. [Graphical view]
SMART	SM00812. Alpha_L_fucos. 1 hit. [Graphical view]
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00385. ALPHA_L_FUCOSIDASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUCO1_ARATH

Accession

Primary (citable) accession number: Q8GW72
Secondary accession number(s): Q9ZUV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	May 3, 2011
Last modified:	October 19, 2011
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program