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Protein

Alpha-L-fucosidase 1

Gene

FUC1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes both 3- and 4-linked fucoses in Lewis determinants. Not active on neither 2-linked fucose nor on fucose in alpha-1,3-linkage to the innermost GlcNAc.1 Publication

Catalytic activityi

An alpha-L-fucoside + H2O = L-fucose + an alcohol.

Kineticsi

  1. KM=28 µM for lacto-N-fucopentaose II1 Publication
  2. KM=6.2 µM for 3-fucosyllactose1 Publication

    pH dependencei

    Optimum pH is 5. Activity decreases sharply toward pH 7.1 Publication

    GO - Molecular functioni

    • alpha-L-fucosidase activity Source: TAIR

    GO - Biological processi

    • carbohydrate metabolic process Source: InterPro
    • glycoprotein catabolic process Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT2G28100-MONOMER.
    MetaCyc:AT2G28100-MONOMER.

    Protein family/group databases

    CAZyiGH29. Glycoside Hydrolase Family 29.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-L-fucosidase 1 (EC:3.2.1.51)
    Alternative name(s):
    Alpha-1,3/4-fucosidase
    Short name:
    AtFUC1
    Alpha-L-fucoside fucohydrolase
    Gene namesi
    Name:FUC1
    Ordered Locus Names:At2g28100
    ORF Names:F24D13.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548 Componenti: Chromosome 2

    Organism-specific databases

    TAIRiAT2G28100.

    Subcellular locationi

    GO - Cellular componenti

    • apoplast Source: UniProtKB-SubCell
    • vacuole Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Apoplast, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 506483Alpha-L-fucosidase 1PRO_0000225692Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi22 – 221N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi82 – 821N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi248 – 2481N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi320 – 3201N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi355 – 3551N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi487 – 4871N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ8GW72.
    PRIDEiQ8GW72.

    Interactioni

    Protein-protein interaction databases

    IntActiQ8GW72. 1 interaction.
    STRINGi3702.AT2G28100.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8GW72.
    SMRiQ8GW72. Positions 38-472.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 29 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3669.
    HOGENOMiHOG000221495.
    InParanoidiQ8GW72.
    KOiK01206.
    OMAiKVRVMIT.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR008979. Galactose-bd-like.
    IPR000933. Glyco_hydro_29.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10030. PTHR10030. 1 hit.
    PfamiPF01120. Alpha_L_fucos. 1 hit.
    [Graphical view]
    SMARTiSM00812. Alpha_L_fucos. 1 hit.
    [Graphical view]
    SUPFAMiSSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8GW72-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MNSQITLFFF FFSILSLSQI SNSSSLLKPH PCPILPLPSS QQLQWQLGSM
    60 70 80 90 100
    AMFLHFGPNT FTDSEWGTGK ANPSIFNPTH LNASQWVQIA KDSGFSRVIL
    110 120 130 140 150
    TAKHHDGFCL WPSEYTDYSV KSSQWRNGAG DVVAELASAA KEAGIGLGLY
    160 170 180 190 200
    LSPWDRHEQC YGKTLEYNEF YLSQMTELLT KYGEIKEVWL DGAKGDGEKD
    210 220 230 240 250
    MEYFFDTWFS LIHQLQPKAV IFSDAGPDVR WIGDEAGLAG STCWSLFNRT
    260 270 280 290 300
    NAKIGDTEPS YSQEGDGYGQ DWVPAECDVS IRPGWFWHAS ESPKPAVQLL
    310 320 330 340 350
    DIYYNSVGRN CLFLLNVPPN SSGLISEQDI KVLEEFSEMK NSIFSNNLAR
    360 370 380 390 400
    KAFVNSSSIR GDQSSQFGPK NVLEEGLDKY WAPEENQNEW VLYLEFKDLV
    410 420 430 440 450
    SFNVLEIREP IHMGQRIASF HLETRKTGSG EWERVVSGTT VGNKRLLRFL
    460 470 480 490 500
    NVVESRSLKL VVDKARTDPL ISYLGLYMDK FSGSSRNTTK ITITRTLKEE

    QQLHDL
    Length:506
    Mass (Da):57,186
    Last modified:May 3, 2011 - v2
    Checksum:i5DD0B508E4560CB9
    GO

    Sequence cautioni

    The sequence AAC98456.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti135 – 1351E → G in BAC43615 (PubMed:11910074).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC005851 Genomic DNA. Translation: AAC98456.1. Sequence problems.
    CP002685 Genomic DNA. Translation: AEC08079.1.
    AK119039 mRNA. Translation: BAC43615.1.
    PIRiG84680.
    RefSeqiNP_180377.2. NM_128370.3.
    UniGeneiAt.38664.
    At.66367.

    Genome annotation databases

    EnsemblPlantsiAT2G28100.1; AT2G28100.1; AT2G28100.
    GeneIDi817355.
    KEGGiath:AT2G28100.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC005851 Genomic DNA. Translation: AAC98456.1. Sequence problems.
    CP002685 Genomic DNA. Translation: AEC08079.1.
    AK119039 mRNA. Translation: BAC43615.1.
    PIRiG84680.
    RefSeqiNP_180377.2. NM_128370.3.
    UniGeneiAt.38664.
    At.66367.

    3D structure databases

    ProteinModelPortaliQ8GW72.
    SMRiQ8GW72. Positions 38-472.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    IntActiQ8GW72. 1 interaction.
    STRINGi3702.AT2G28100.1.

    Protein family/group databases

    CAZyiGH29. Glycoside Hydrolase Family 29.

    Proteomic databases

    PaxDbiQ8GW72.
    PRIDEiQ8GW72.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT2G28100.1; AT2G28100.1; AT2G28100.
    GeneIDi817355.
    KEGGiath:AT2G28100.

    Organism-specific databases

    TAIRiAT2G28100.

    Phylogenomic databases

    eggNOGiCOG3669.
    HOGENOMiHOG000221495.
    InParanoidiQ8GW72.
    KOiK01206.
    OMAiKVRVMIT.

    Enzyme and pathway databases

    BioCyciARA:AT2G28100-MONOMER.
    MetaCyc:AT2G28100-MONOMER.

    Miscellaneous databases

    PROiQ8GW72.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR008979. Galactose-bd-like.
    IPR000933. Glyco_hydro_29.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10030. PTHR10030. 1 hit.
    PfamiPF01120. Alpha_L_fucos. 1 hit.
    [Graphical view]
    SMARTiSM00812. Alpha_L_fucos. 1 hit.
    [Graphical view]
    SUPFAMiSSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "AtFXG1, an Arabidopsis gene encoding alpha-L-fucosidase active against fucosylated xyloglucan oligosaccharides."
      de La Torre F., Sampedro J., Zarra I., Revilla G.
      Plant Physiol. 128:247-255(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY FUNCTION, SUBCELLULAR LOCATION.
    5. "Molecular cloning and characterization of a plant alpha1,3/4-fucosidase based on sequence tags from almond fucosidase I."
      Zeleny R., Leonard R., Dorfner G., Dalik T., Kolarich D., Altmann F.
      Phytochemistry 67:641-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiFUCO1_ARATH
    AccessioniPrimary (citable) accession number: Q8GW72
    Secondary accession number(s): Q9ZUV2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 7, 2006
    Last sequence update: May 3, 2011
    Last modified: July 22, 2015
    This is version 90 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Caution

    Was reported by PubMed:11788770 to be an alpha-1,2-fucosidase.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.