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Q8GW72

- FUCO1_ARATH

UniProt

Q8GW72 - FUCO1_ARATH

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Protein

Alpha-L-fucosidase 1

Gene
FUC1, At2g28100, F24D13.11
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes both 3- and 4-linked fucoses in Lewis determinants. Not active on neither 2-linked fucose nor on fucose in alpha-1,3-linkage to the innermost GlcNAc.2 Publications

Catalytic activityi

An alpha-L-fucoside + H2O = L-fucose + an alcohol.

Kineticsi

  1. KM=28 µM for lacto-N-fucopentaose II1 Publication
  2. KM=6.2 µM for 3-fucosyllactose

pH dependencei

Optimum pH is 5. Activity decreases sharply toward pH 7.

GO - Molecular functioni

  1. alpha-L-fucosidase activity Source: TAIR

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. glycoprotein catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT2G28100-MONOMER.
MetaCyc:AT2G28100-MONOMER.

Protein family/group databases

CAZyiGH29. Glycoside Hydrolase Family 29.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-L-fucosidase 1 (EC:3.2.1.51)
Alternative name(s):
Alpha-1,3/4-fucosidase
Short name:
AtFUC1
Alpha-L-fucoside fucohydrolase
Gene namesi
Name:FUC1
Ordered Locus Names:At2g28100
ORF Names:F24D13.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G28100.

Subcellular locationi

Secretedextracellular spaceapoplast 1 Publication

GO - Cellular componenti

  1. apoplast Source: UniProtKB-SubCell
  2. vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323 Reviewed predictionAdd
BLAST
Chaini24 – 506483Alpha-L-fucosidase 1PRO_0000225692Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi22 – 221N-linked (GlcNAc...) Reviewed prediction
Glycosylationi82 – 821N-linked (GlcNAc...) Reviewed prediction
Glycosylationi248 – 2481N-linked (GlcNAc...) Reviewed prediction
Glycosylationi320 – 3201N-linked (GlcNAc...) Reviewed prediction
Glycosylationi355 – 3551N-linked (GlcNAc...) Reviewed prediction
Glycosylationi487 – 4871N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8GW72.
PRIDEiQ8GW72.

Expressioni

Gene expression databases

GenevestigatoriQ8GW72.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
BRL1Q9ZPS91EBI-2293793,EBI-2292728

Protein-protein interaction databases

IntActiQ8GW72. 1 interaction.
STRINGi3702.AT2G28100.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8GW72.
SMRiQ8GW72. Positions 38-472.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3669.
HOGENOMiHOG000221495.
InParanoidiQ8GW72.
KOiK01206.
OMAiYWIANEN.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR000933. Glyco_hydro_29.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10030. PTHR10030. 1 hit.
PfamiPF01120. Alpha_L_fucos. 1 hit.
[Graphical view]
SMARTiSM00812. Alpha_L_fucos. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8GW72-1 [UniParc]FASTAAdd to Basket

« Hide

MNSQITLFFF FFSILSLSQI SNSSSLLKPH PCPILPLPSS QQLQWQLGSM    50
AMFLHFGPNT FTDSEWGTGK ANPSIFNPTH LNASQWVQIA KDSGFSRVIL 100
TAKHHDGFCL WPSEYTDYSV KSSQWRNGAG DVVAELASAA KEAGIGLGLY 150
LSPWDRHEQC YGKTLEYNEF YLSQMTELLT KYGEIKEVWL DGAKGDGEKD 200
MEYFFDTWFS LIHQLQPKAV IFSDAGPDVR WIGDEAGLAG STCWSLFNRT 250
NAKIGDTEPS YSQEGDGYGQ DWVPAECDVS IRPGWFWHAS ESPKPAVQLL 300
DIYYNSVGRN CLFLLNVPPN SSGLISEQDI KVLEEFSEMK NSIFSNNLAR 350
KAFVNSSSIR GDQSSQFGPK NVLEEGLDKY WAPEENQNEW VLYLEFKDLV 400
SFNVLEIREP IHMGQRIASF HLETRKTGSG EWERVVSGTT VGNKRLLRFL 450
NVVESRSLKL VVDKARTDPL ISYLGLYMDK FSGSSRNTTK ITITRTLKEE 500
QQLHDL 506
Length:506
Mass (Da):57,186
Last modified:May 3, 2011 - v2
Checksum:i5DD0B508E4560CB9
GO

Sequence cautioni

The sequence AAC98456.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti135 – 1351E → G in BAC43615. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC005851 Genomic DNA. Translation: AAC98456.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC08079.1.
AK119039 mRNA. Translation: BAC43615.1.
PIRiG84680.
RefSeqiNP_180377.2. NM_128370.3.
UniGeneiAt.38664.
At.66367.

Genome annotation databases

EnsemblPlantsiAT2G28100.1; AT2G28100.1; AT2G28100.
GeneIDi817355.
KEGGiath:AT2G28100.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC005851 Genomic DNA. Translation: AAC98456.1 . Sequence problems.
CP002685 Genomic DNA. Translation: AEC08079.1 .
AK119039 mRNA. Translation: BAC43615.1 .
PIRi G84680.
RefSeqi NP_180377.2. NM_128370.3.
UniGenei At.38664.
At.66367.

3D structure databases

ProteinModelPortali Q8GW72.
SMRi Q8GW72. Positions 38-472.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8GW72. 1 interaction.
STRINGi 3702.AT2G28100.1-P.

Protein family/group databases

CAZyi GH29. Glycoside Hydrolase Family 29.

Proteomic databases

PaxDbi Q8GW72.
PRIDEi Q8GW72.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G28100.1 ; AT2G28100.1 ; AT2G28100 .
GeneIDi 817355.
KEGGi ath:AT2G28100.

Organism-specific databases

TAIRi AT2G28100.

Phylogenomic databases

eggNOGi COG3669.
HOGENOMi HOG000221495.
InParanoidi Q8GW72.
KOi K01206.
OMAi YWIANEN.

Enzyme and pathway databases

BioCyci ARA:AT2G28100-MONOMER.
MetaCyc:AT2G28100-MONOMER.

Gene expression databases

Genevestigatori Q8GW72.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR008979. Galactose-bd-like.
IPR000933. Glyco_hydro_29.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10030. PTHR10030. 1 hit.
Pfami PF01120. Alpha_L_fucos. 1 hit.
[Graphical view ]
SMARTi SM00812. Alpha_L_fucos. 1 hit.
[Graphical view ]
SUPFAMi SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "AtFXG1, an Arabidopsis gene encoding alpha-L-fucosidase active against fucosylated xyloglucan oligosaccharides."
    de La Torre F., Sampedro J., Zarra I., Revilla G.
    Plant Physiol. 128:247-255(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY FUNCTION, SUBCELLULAR LOCATION.
  5. "Molecular cloning and characterization of a plant alpha1,3/4-fucosidase based on sequence tags from almond fucosidase I."
    Zeleny R., Leonard R., Dorfner G., Dalik T., Kolarich D., Altmann F.
    Phytochemistry 67:641-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiFUCO1_ARATH
AccessioniPrimary (citable) accession number: Q8GW72
Secondary accession number(s): Q9ZUV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: May 3, 2011
Last modified: May 14, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Was reported by 1 Publication to be an alpha-1,2-fucosidase.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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