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Q8GW72 (FUCO1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-L-fucosidase 1

EC=3.2.1.51
Alternative name(s):
Alpha-1,3/4-fucosidase
Short name=AtFUC1
Alpha-L-fucoside fucohydrolase
Gene names
Name:FUC1
Ordered Locus Names:At2g28100
ORF Names:F24D13.11
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length506 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes both 3- and 4-linked fucoses in Lewis determinants. Not active on neither 2-linked fucose nor on fucose in alpha-1,3-linkage to the innermost GlcNAc. Ref.4 Ref.5

Catalytic activity

An alpha-L-fucoside + H2O = L-fucose + an alcohol.

Subcellular location

Secretedextracellular spaceapoplast Ref.4.

Sequence similarities

Belongs to the glycosyl hydrolase 29 family.

Caution

Was reported by Ref.4 to be an alpha-1,2-fucosidase.

Biophysicochemical properties

Kinetic parameters:

KM=28 µM for lacto-N-fucopentaose II Ref.5

KM=6.2 µM for 3-fucosyllactose

pH dependence:

Optimum pH is 5. Activity decreases sharply toward pH 7.

Sequence caution

The sequence AAC98456.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentApoplast
Secreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

glycoprotein catabolic process

Traceable author statement Ref.5. Source: TAIR

   Cellular_componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

vacuole

Inferred from direct assay PubMed 15539469. Source: TAIR

   Molecular_functionalpha-L-fucosidase activity

Inferred from direct assay Ref.4Ref.5. Source: TAIR

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

BRL1Q9ZPS91EBI-2293793,EBI-2292728

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 506483Alpha-L-fucosidase 1
PRO_0000225692

Amino acid modifications

Glycosylation221N-linked (GlcNAc...) Potential
Glycosylation821N-linked (GlcNAc...) Potential
Glycosylation2481N-linked (GlcNAc...) Potential
Glycosylation3201N-linked (GlcNAc...) Potential
Glycosylation3551N-linked (GlcNAc...) Potential
Glycosylation4871N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict1351E → G in BAC43615. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q8GW72 [UniParc].

Last modified May 3, 2011. Version 2.
Checksum: 5DD0B508E4560CB9

FASTA50657,186
        10         20         30         40         50         60 
MNSQITLFFF FFSILSLSQI SNSSSLLKPH PCPILPLPSS QQLQWQLGSM AMFLHFGPNT 

        70         80         90        100        110        120 
FTDSEWGTGK ANPSIFNPTH LNASQWVQIA KDSGFSRVIL TAKHHDGFCL WPSEYTDYSV 

       130        140        150        160        170        180 
KSSQWRNGAG DVVAELASAA KEAGIGLGLY LSPWDRHEQC YGKTLEYNEF YLSQMTELLT 

       190        200        210        220        230        240 
KYGEIKEVWL DGAKGDGEKD MEYFFDTWFS LIHQLQPKAV IFSDAGPDVR WIGDEAGLAG 

       250        260        270        280        290        300 
STCWSLFNRT NAKIGDTEPS YSQEGDGYGQ DWVPAECDVS IRPGWFWHAS ESPKPAVQLL 

       310        320        330        340        350        360 
DIYYNSVGRN CLFLLNVPPN SSGLISEQDI KVLEEFSEMK NSIFSNNLAR KAFVNSSSIR 

       370        380        390        400        410        420 
GDQSSQFGPK NVLEEGLDKY WAPEENQNEW VLYLEFKDLV SFNVLEIREP IHMGQRIASF 

       430        440        450        460        470        480 
HLETRKTGSG EWERVVSGTT VGNKRLLRFL NVVESRSLKL VVDKARTDPL ISYLGLYMDK 

       490        500 
FSGSSRNTTK ITITRTLKEE QQLHDL 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Functional annotation of a full-length Arabidopsis cDNA collection."
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.
Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"AtFXG1, an Arabidopsis gene encoding alpha-L-fucosidase active against fucosylated xyloglucan oligosaccharides."
de La Torre F., Sampedro J., Zarra I., Revilla G.
Plant Physiol. 128:247-255(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY FUNCTION, SUBCELLULAR LOCATION.
[5]"Molecular cloning and characterization of a plant alpha1,3/4-fucosidase based on sequence tags from almond fucosidase I."
Zeleny R., Leonard R., Dorfner G., Dalik T., Kolarich D., Altmann F.
Phytochemistry 67:641-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC005851 Genomic DNA. Translation: AAC98456.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC08079.1.
AK119039 mRNA. Translation: BAC43615.1.
PIRG84680.
RefSeqNP_180377.2. NM_128370.3.
UniGeneAt.38664.
At.66367.

3D structure databases

ProteinModelPortalQ8GW72.
SMRQ8GW72. Positions 38-472.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8GW72. 1 interaction.
STRING3702.AT2G28100.1-P.

Protein family/group databases

CAZyGH29. Glycoside Hydrolase Family 29.

Proteomic databases

PaxDbQ8GW72.
PRIDEQ8GW72.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G28100.1; AT2G28100.1; AT2G28100.
GeneID817355.
KEGGath:AT2G28100.

Organism-specific databases

TAIRAT2G28100.

Phylogenomic databases

eggNOGCOG3669.
HOGENOMHOG000221495.
InParanoidQ8GW72.
KOK01206.
OMAYWIANEN.

Enzyme and pathway databases

BioCycARA:AT2G28100-MONOMER.
MetaCyc:AT2G28100-MONOMER.

Gene expression databases

GenevestigatorQ8GW72.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProIPR008979. Galactose-bd-like.
IPR000933. Glyco_hydro_29.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10030. PTHR10030. 1 hit.
PfamPF01120. Alpha_L_fucos. 1 hit.
[Graphical view]
SMARTSM00812. Alpha_L_fucos. 1 hit.
[Graphical view]
SUPFAMSSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameFUCO1_ARATH
AccessionPrimary (citable) accession number: Q8GW72
Secondary accession number(s): Q9ZUV2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: May 3, 2011
Last modified: May 14, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names