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Protein

Alpha-L-fucosidase 1

Gene

FUC1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes both 3- and 4-linked fucoses in Lewis determinants. Not active on neither 2-linked fucose nor on fucose in alpha-1,3-linkage to the innermost GlcNAc.1 Publication

Catalytic activityi

An alpha-L-fucoside + H2O = L-fucose + an alcohol.

Kineticsi

  1. KM=28 µM for lacto-N-fucopentaose II1 Publication
  2. KM=6.2 µM for 3-fucosyllactose1 Publication

pH dependencei

Optimum pH is 5. Activity decreases sharply toward pH 7.1 Publication

GO - Molecular functioni

  1. alpha-L-fucosidase activity Source: TAIR
  2. fucose binding Source: GO_Central

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. glycoprotein catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT2G28100-MONOMER.
MetaCyc:AT2G28100-MONOMER.

Protein family/group databases

CAZyiGH29. Glycoside Hydrolase Family 29.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-L-fucosidase 1 (EC:3.2.1.51)
Alternative name(s):
Alpha-1,3/4-fucosidase
Short name:
AtFUC1
Alpha-L-fucoside fucohydrolase
Gene namesi
Name:FUC1
Ordered Locus Names:At2g28100
ORF Names:F24D13.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G28100.

Subcellular locationi

Secretedextracellular spaceapoplast 1 Publication

GO - Cellular componenti

  1. apoplast Source: UniProtKB-SubCell
  2. vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 506483Alpha-L-fucosidase 1PRO_0000225692Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi22 – 221N-linked (GlcNAc...)Sequence Analysis
Glycosylationi82 – 821N-linked (GlcNAc...)Sequence Analysis
Glycosylationi248 – 2481N-linked (GlcNAc...)Sequence Analysis
Glycosylationi320 – 3201N-linked (GlcNAc...)Sequence Analysis
Glycosylationi355 – 3551N-linked (GlcNAc...)Sequence Analysis
Glycosylationi487 – 4871N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8GW72.
PRIDEiQ8GW72.

Expressioni

Gene expression databases

GenevestigatoriQ8GW72.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
BRL1Q9ZPS91EBI-2293793,EBI-2292728

Protein-protein interaction databases

IntActiQ8GW72. 1 interaction.
STRINGi3702.AT2G28100.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8GW72.
SMRiQ8GW72. Positions 38-472.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 29 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3669.
HOGENOMiHOG000221495.
InParanoidiQ8GW72.
KOiK01206.
OMAiLQEPIQL.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR000933. Glyco_hydro_29.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10030. PTHR10030. 1 hit.
PfamiPF01120. Alpha_L_fucos. 1 hit.
[Graphical view]
SMARTiSM00812. Alpha_L_fucos. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8GW72-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSQITLFFF FFSILSLSQI SNSSSLLKPH PCPILPLPSS QQLQWQLGSM
60 70 80 90 100
AMFLHFGPNT FTDSEWGTGK ANPSIFNPTH LNASQWVQIA KDSGFSRVIL
110 120 130 140 150
TAKHHDGFCL WPSEYTDYSV KSSQWRNGAG DVVAELASAA KEAGIGLGLY
160 170 180 190 200
LSPWDRHEQC YGKTLEYNEF YLSQMTELLT KYGEIKEVWL DGAKGDGEKD
210 220 230 240 250
MEYFFDTWFS LIHQLQPKAV IFSDAGPDVR WIGDEAGLAG STCWSLFNRT
260 270 280 290 300
NAKIGDTEPS YSQEGDGYGQ DWVPAECDVS IRPGWFWHAS ESPKPAVQLL
310 320 330 340 350
DIYYNSVGRN CLFLLNVPPN SSGLISEQDI KVLEEFSEMK NSIFSNNLAR
360 370 380 390 400
KAFVNSSSIR GDQSSQFGPK NVLEEGLDKY WAPEENQNEW VLYLEFKDLV
410 420 430 440 450
SFNVLEIREP IHMGQRIASF HLETRKTGSG EWERVVSGTT VGNKRLLRFL
460 470 480 490 500
NVVESRSLKL VVDKARTDPL ISYLGLYMDK FSGSSRNTTK ITITRTLKEE

QQLHDL
Length:506
Mass (Da):57,186
Last modified:May 2, 2011 - v2
Checksum:i5DD0B508E4560CB9
GO

Sequence cautioni

The sequence AAC98456.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti135 – 1351E → G in BAC43615 (PubMed:11910074).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005851 Genomic DNA. Translation: AAC98456.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC08079.1.
AK119039 mRNA. Translation: BAC43615.1.
PIRiG84680.
RefSeqiNP_180377.2. NM_128370.3.
UniGeneiAt.38664.
At.66367.

Genome annotation databases

EnsemblPlantsiAT2G28100.1; AT2G28100.1; AT2G28100.
GeneIDi817355.
KEGGiath:AT2G28100.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005851 Genomic DNA. Translation: AAC98456.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC08079.1.
AK119039 mRNA. Translation: BAC43615.1.
PIRiG84680.
RefSeqiNP_180377.2. NM_128370.3.
UniGeneiAt.38664.
At.66367.

3D structure databases

ProteinModelPortaliQ8GW72.
SMRiQ8GW72. Positions 38-472.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8GW72. 1 interaction.
STRINGi3702.AT2G28100.1-P.

Protein family/group databases

CAZyiGH29. Glycoside Hydrolase Family 29.

Proteomic databases

PaxDbiQ8GW72.
PRIDEiQ8GW72.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G28100.1; AT2G28100.1; AT2G28100.
GeneIDi817355.
KEGGiath:AT2G28100.

Organism-specific databases

TAIRiAT2G28100.

Phylogenomic databases

eggNOGiCOG3669.
HOGENOMiHOG000221495.
InParanoidiQ8GW72.
KOiK01206.
OMAiLQEPIQL.

Enzyme and pathway databases

BioCyciARA:AT2G28100-MONOMER.
MetaCyc:AT2G28100-MONOMER.

Gene expression databases

GenevestigatoriQ8GW72.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR000933. Glyco_hydro_29.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10030. PTHR10030. 1 hit.
PfamiPF01120. Alpha_L_fucos. 1 hit.
[Graphical view]
SMARTiSM00812. Alpha_L_fucos. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "AtFXG1, an Arabidopsis gene encoding alpha-L-fucosidase active against fucosylated xyloglucan oligosaccharides."
    de La Torre F., Sampedro J., Zarra I., Revilla G.
    Plant Physiol. 128:247-255(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY FUNCTION, SUBCELLULAR LOCATION.
  5. "Molecular cloning and characterization of a plant alpha1,3/4-fucosidase based on sequence tags from almond fucosidase I."
    Zeleny R., Leonard R., Dorfner G., Dalik T., Kolarich D., Altmann F.
    Phytochemistry 67:641-648(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiFUCO1_ARATH
AccessioniPrimary (citable) accession number: Q8GW72
Secondary accession number(s): Q9ZUV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2006
Last sequence update: May 2, 2011
Last modified: February 3, 2015
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Was reported by PubMed:11788770 to be an alpha-1,2-fucosidase.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.