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Protein

Alpha-L-fucosidase 1

Gene

FUC1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes both 3- and 4-linked fucoses in Lewis determinants. Not active on neither 2-linked fucose nor on fucose in alpha-1,3-linkage to the innermost GlcNAc.1 Publication

Caution

Was reported by PubMed:11788770 to be an alpha-1,2-fucosidase.Curated

Catalytic activityi

An alpha-L-fucoside + H2O = L-fucose + an alcohol.

Kineticsi

  1. KM=28 µM for lacto-N-fucopentaose II1 Publication
  2. KM=6.2 µM for 3-fucosyllactose1 Publication

    pH dependencei

    Optimum pH is 5. Activity decreases sharply toward pH 7.1 Publication

    GO - Molecular functioni

    • alpha-L-fucosidase activity Source: TAIR

    GO - Biological processi

    • carbohydrate metabolic process Source: InterPro
    • glycoprotein catabolic process Source: TAIR

    Keywordsi

    Molecular functionGlycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT2G28100-MONOMER
    MetaCyc:AT2G28100-MONOMER

    Protein family/group databases

    CAZyiGH29 Glycoside Hydrolase Family 29

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-L-fucosidase 1 (EC:3.2.1.51)
    Alternative name(s):
    Alpha-1,3/4-fucosidase
    Short name:
    AtFUC1
    Alpha-L-fucoside fucohydrolase
    Gene namesi
    Name:FUC1
    Ordered Locus Names:At2g28100
    ORF Names:F24D13.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 2

    Organism-specific databases

    AraportiAT2G28100
    TAIRilocus:2046173 AT2G28100

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Apoplast, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 23Sequence analysisAdd BLAST23
    ChainiPRO_000022569224 – 506Alpha-L-fucosidase 1Add BLAST483

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi22N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi82N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi248N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi320N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi355N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi487N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ8GW72
    PRIDEiQ8GW72

    PTM databases

    iPTMnetiQ8GW72

    Expressioni

    Gene expression databases

    ExpressionAtlasiQ8GW72 baseline and differential
    GenevisibleiQ8GW72 AT

    Interactioni

    Protein-protein interaction databases

    BioGridi2705, 1 interactor
    IntActiQ8GW72, 1 interactor
    STRINGi3702.AT2G28100.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ8GW72
    SMRiQ8GW72
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 29 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiKOG3340 Eukaryota
    COG3669 LUCA
    HOGENOMiHOG000221495
    InParanoidiQ8GW72
    KOiK01206
    OMAiKVRVMIT
    OrthoDBiEOG09360DA6

    Family and domain databases

    Gene3Di2.60.120.260, 1 hit
    InterProiView protein in InterPro
    IPR008979 Galactose-bd-like_sf
    IPR000933 Glyco_hydro_29
    IPR017853 Glycoside_hydrolase_SF
    PANTHERiPTHR10030 PTHR10030, 1 hit
    PfamiView protein in Pfam
    PF01120 Alpha_L_fucos, 1 hit
    SMARTiView protein in SMART
    SM00812 Alpha_L_fucos, 1 hit
    SUPFAMiSSF49785 SSF49785, 1 hit
    SSF51445 SSF51445, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8GW72-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MNSQITLFFF FFSILSLSQI SNSSSLLKPH PCPILPLPSS QQLQWQLGSM
    60 70 80 90 100
    AMFLHFGPNT FTDSEWGTGK ANPSIFNPTH LNASQWVQIA KDSGFSRVIL
    110 120 130 140 150
    TAKHHDGFCL WPSEYTDYSV KSSQWRNGAG DVVAELASAA KEAGIGLGLY
    160 170 180 190 200
    LSPWDRHEQC YGKTLEYNEF YLSQMTELLT KYGEIKEVWL DGAKGDGEKD
    210 220 230 240 250
    MEYFFDTWFS LIHQLQPKAV IFSDAGPDVR WIGDEAGLAG STCWSLFNRT
    260 270 280 290 300
    NAKIGDTEPS YSQEGDGYGQ DWVPAECDVS IRPGWFWHAS ESPKPAVQLL
    310 320 330 340 350
    DIYYNSVGRN CLFLLNVPPN SSGLISEQDI KVLEEFSEMK NSIFSNNLAR
    360 370 380 390 400
    KAFVNSSSIR GDQSSQFGPK NVLEEGLDKY WAPEENQNEW VLYLEFKDLV
    410 420 430 440 450
    SFNVLEIREP IHMGQRIASF HLETRKTGSG EWERVVSGTT VGNKRLLRFL
    460 470 480 490 500
    NVVESRSLKL VVDKARTDPL ISYLGLYMDK FSGSSRNTTK ITITRTLKEE

    QQLHDL
    Length:506
    Mass (Da):57,186
    Last modified:May 3, 2011 - v2
    Checksum:i5DD0B508E4560CB9
    GO

    Sequence cautioni

    The sequence AAC98456 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti135E → G in BAC43615 (PubMed:11910074).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC005851 Genomic DNA Translation: AAC98456.1 Sequence problems.
    CP002685 Genomic DNA Translation: AEC08079.1
    AK119039 mRNA Translation: BAC43615.1
    PIRiG84680
    RefSeqiNP_180377.2, NM_128370.3
    UniGeneiAt.38664
    At.66367

    Genome annotation databases

    EnsemblPlantsiAT2G28100.1; AT2G28100.1; AT2G28100
    GeneIDi817355
    GrameneiAT2G28100.1; AT2G28100.1; AT2G28100
    KEGGiath:AT2G28100

    Similar proteinsi

    Entry informationi

    Entry nameiFUCO1_ARATH
    AccessioniPrimary (citable) accession number: Q8GW72
    Secondary accession number(s): Q9ZUV2
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 7, 2006
    Last sequence update: May 3, 2011
    Last modified: May 23, 2018
    This is version 102 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

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