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Protein

Peptidyl-tRNA hydrolase, chloroplastic

Gene

At1g18440

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.By similarity

Catalytic activityi

N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciARA:AT1G18440-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidyl-tRNA hydrolase, chloroplastic (EC:3.1.1.29)
Alternative name(s):
C-PTH
Gene namesi
Ordered Locus Names:At1g18440
ORF Names:F15H18.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G18440.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: UniProtKB-SubCell
  • mitochondrion Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 55ChloroplastSequence analysisAdd BLAST55
ChainiPRO_000028052756 – 288Peptidyl-tRNA hydrolase, chloroplasticAdd BLAST233

Proteomic databases

PaxDbiQ8GW64.

Expressioni

Gene expression databases

GenevisibleiQ8GW64. AT.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi23663. 1 interactor.
STRINGi3702.AT1G18440.1.

Structurei

3D structure databases

ProteinModelPortaliQ8GW64.
SMRiQ8GW64.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi52 – 88Ser-richAdd BLAST37

Sequence similaritiesi

Belongs to the PTH family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2255. Eukaryota.
COG0193. LUCA.
HOGENOMiHOG000004797.
InParanoidiQ8GW64.
KOiK01056.
OMAiNTVNFKA.
OrthoDBiEOG09360L5D.
PhylomeDBiQ8GW64.

Family and domain databases

Gene3Di3.40.50.1470. 1 hit.
HAMAPiMF_00083. Pept_tRNA_hydro_bact. 1 hit.
InterProiIPR001328. Pept_tRNA_hydro.
IPR018171. Pept_tRNA_hydro_CS.
[Graphical view]
PANTHERiPTHR17224. PTHR17224. 1 hit.
PfamiPF01195. Pept_tRNA_hydro. 1 hit.
[Graphical view]
SUPFAMiSSF53178. SSF53178. 1 hit.
TIGRFAMsiTIGR00447. pth. 1 hit.
PROSITEiPS01195. PEPT_TRNA_HYDROL_1. 1 hit.
PS01196. PEPT_TRNA_HYDROL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8GW64-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAVAFPAKI ANLSFPSNCC SLFFRSPATF LSPALPCRKL TKGIRGLEGL
60 70 80 90 100
MSQCLSSSSQ SLSFSSNSFS SQPESELLQA LPSSKPKSPP LQLPWLIVGL
110 120 130 140 150
GNPGKKYQGT RHNVGFEMVD ALADAEGISM NTVNFKALFG KGVIGNIPIM
160 170 180 190 200
LAKPQTFMNL SGESVGQIVS FYKIPLKQVL VVYDDLDLPF GKLRLLPKGG
210 220 230 240 250
HGGHNGMRSI IDRLKGSRDF PRLRIGIGRP PGKMDTANFV LRQFNRQEQE
260 270 280
ELDHTFQTGL EAIRILLLEG FNKSATFVNT RKSMEQLS
Length:288
Mass (Da):31,562
Last modified:March 20, 2007 - v2
Checksum:i9CD118CA2915DD0F
GO

Sequence cautioni

The sequence AAF25998 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene has been split into 2 genes: At1g18440 and At1g18450.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti159N → D in BAC43630 (PubMed:11910074).Curated1
Sequence conflicti159N → D in AAO63348 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC013354 Genomic DNA. Translation: AAF25998.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29714.1.
AK119054 mRNA. Translation: BAC43630.1.
BT005284 mRNA. Translation: AAO63348.1.
PIRiC86318.
RefSeqiNP_173279.2. NM_101701.4.
UniGeneiAt.22357.

Genome annotation databases

EnsemblPlantsiAT1G18440.1; AT1G18440.1; AT1G18440.
GeneIDi838424.
GrameneiAT1G18440.1; AT1G18440.1; AT1G18440.
KEGGiath:AT1G18440.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC013354 Genomic DNA. Translation: AAF25998.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29714.1.
AK119054 mRNA. Translation: BAC43630.1.
BT005284 mRNA. Translation: AAO63348.1.
PIRiC86318.
RefSeqiNP_173279.2. NM_101701.4.
UniGeneiAt.22357.

3D structure databases

ProteinModelPortaliQ8GW64.
SMRiQ8GW64.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23663. 1 interactor.
STRINGi3702.AT1G18440.1.

Proteomic databases

PaxDbiQ8GW64.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G18440.1; AT1G18440.1; AT1G18440.
GeneIDi838424.
GrameneiAT1G18440.1; AT1G18440.1; AT1G18440.
KEGGiath:AT1G18440.

Organism-specific databases

TAIRiAT1G18440.

Phylogenomic databases

eggNOGiKOG2255. Eukaryota.
COG0193. LUCA.
HOGENOMiHOG000004797.
InParanoidiQ8GW64.
KOiK01056.
OMAiNTVNFKA.
OrthoDBiEOG09360L5D.
PhylomeDBiQ8GW64.

Enzyme and pathway databases

BioCyciARA:AT1G18440-MONOMER.

Miscellaneous databases

PROiQ8GW64.

Gene expression databases

GenevisibleiQ8GW64. AT.

Family and domain databases

Gene3Di3.40.50.1470. 1 hit.
HAMAPiMF_00083. Pept_tRNA_hydro_bact. 1 hit.
InterProiIPR001328. Pept_tRNA_hydro.
IPR018171. Pept_tRNA_hydro_CS.
[Graphical view]
PANTHERiPTHR17224. PTHR17224. 1 hit.
PfamiPF01195. Pept_tRNA_hydro. 1 hit.
[Graphical view]
SUPFAMiSSF53178. SSF53178. 1 hit.
TIGRFAMsiTIGR00447. pth. 1 hit.
PROSITEiPS01195. PEPT_TRNA_HYDROL_1. 1 hit.
PS01196. PEPT_TRNA_HYDROL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTHC_ARATH
AccessioniPrimary (citable) accession number: Q8GW64
Secondary accession number(s): Q9LPQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: March 20, 2007
Last modified: November 30, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.