Reviewed, UniProtKB/Swiss-Prot Q8GVF9 (4CLL8_ORYSJ)

Names and origin

Protein names

Recommended name:
    Putative 4-coumarate--CoA ligase-like 8
    EC=6.2.1.-

Gene names

Name:	4CLL8
Ordered Locus Names:	Os07g0639100, LOC_Os07g44560
ORF Names:	OJ1340_C08.126

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	609 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Domain	Both substrate-binding domains (SBD1 and SBD2) are involved in the substrate recognition, and are sufficient to confer the substrate specificity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ligase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 609	609	Putative 4-coumarate--CoA ligase-like 8		PRO_0000351634
Regions
	Nucleotide binding	194 – 202	9	ATP By similarity
	Nucleotide binding	349 – 354	6	ATP By similarity
	Region	276 – 348	73	SBD1
	Region	349 – 450	102	SBD2
Sites
	Binding site	482	1	ATP By similarity
	Binding site	497	1	ATP By similarity
	Binding site	589	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8GVF9-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 75FE211B56CA8557	FASTA	609	64,509
	10 20 30 40 50 60 METELHLAAG YCAATGVYRS GHPPQFAAAA ALSFPEYILP HMLLPGRRAR PAFVDASTGA 70 80 90 100 110 120 ALSFAGLRAL SLRVARALAA AGLRRGRVAL LLSPNSLHFP ALSLAVLSLG AVLSAANPLL 130 140 150 160 170 180 TPDELARQAD DAKPFLALVT GELAPKLRSI APDVKLVLVE QLLADVAAEV DDDETLDLPA 190 200 210 220 230 240 ANIGRDDAAL LFYSSGTTGR SKGVVSTHGN AIAMAASLER AWGGGGGGGE KPQQYDDHDE 250 260 270 280 290 300 AYGCVLPMFH MFGFSSFVMG TAALGATAVV VPGRFSVEKT MAAVEEYGVT RLLVVPPMVV 310 320 330 340 350 360 KMVAAAAGDG EPSRRRLRLR QVVSSGAPLQ REHMARFRSC FPAVNLGQCY GLTETTGIVT 370 380 390 400 410 420 MCDLQHNDNG IDKVEMPPSS TDMTFVAVAA TTTEVKERST GGGGGGGGVS IGRLMPDVEA 430 440 450 460 470 480 KIVDPDSGEL LPPRRTGELW VRGPSTMRGY LNNEEATALA LVAAAGSVSV SGGGERWLRT 490 500 510 520 530 540 GDLCYVDSRG LVYVVDRVKE LIKCNAYQVA PAELEDVLAT HPDIHDAAVA PYPDKEAGEI 550 560 570 580 590 600 PMAYVVKKQG SGHLQEDEVI SFVQNKVAPY KKIRKVVFVD SIPRSPSGKI LRRQLKNLLQ GSILHRSRM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Genome-wide analysis of a land plant-specific acyl:coenzyme A synthetase (ACS) gene family in Arabidopsis, poplar, rice and Physcomitrella." de Azevedo Souza C., Barbazuk B., Ralph S.G., Bohlmann J., Hamberger B., Douglas C.J. New Phytol. 179:987-1003(2008) [PubMed: 18627494] [Abstract] Cited for: GENE FAMILY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP005292 Genomic DNA. Translation: BAC45208.1.
3D structure databases
HSSP	HSSP built from PDB template 1LCI based on UniProtKB P08659.
ModBase	Search...
Organism-specific databases
Gramene	Q8GVF9.
Family and domain databases
InterPro	IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

4CLL8_ORYSJ

Accession

Primary (citable) accession number: Q8GVF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	March 1, 2003
Last modified:	March 24, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)