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Q8GVE8

- CAPP4_ARATH

UniProt

Q8GVE8 - CAPP4_ARATH

Protein

Phosphoenolpyruvate carboxylase 4

Gene

PPC4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Through the carboxylation of phosphoenolpyruvate (PEP) it forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.

    Catalytic activityi

    Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.

    Cofactori

    Magnesium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei154 – 1541By similarity
    Active sitei699 – 6991By similarity

    GO - Molecular functioni

    1. phosphoenolpyruvate carboxylase activity Source: TAIR

    GO - Biological processi

    1. carbon fixation Source: UniProtKB-KW
    2. photosynthesis Source: UniProtKB-KW
    3. tricarboxylic acid cycle Source: TAIR

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Carbon dioxide fixation, Photosynthesis

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciARA:AT1G68750-MONOMER.
    BRENDAi4.1.1.31. 399.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxylase 4 (EC:4.1.1.31)
    Short name:
    AtPPC4
    Short name:
    PEPC 4
    Short name:
    PEPCase 4
    Gene namesi
    Name:PPC4
    Ordered Locus Names:At1g68750
    ORF Names:F14K14.14, F24J5.1
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G68750.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10321032Phosphoenolpyruvate carboxylase 4PRO_0000166660Add
    BLAST

    Proteomic databases

    PaxDbiQ8GVE8.
    PRIDEiQ8GVE8.

    Expressioni

    Tissue specificityi

    Expressed at low levels in flowers and siliques, and detectable in roots.1 Publication

    Gene expression databases

    GenevestigatoriQ8GVE8.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ8GVE8.
    SMRiQ8GVE8. Positions 14-322, 446-1032.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PEPCase type 1 family.Curated

    Phylogenomic databases

    eggNOGiCOG2352.
    HOGENOMiHOG000238648.
    InParanoidiQ8GVE8.
    KOiK01595.
    OMAiISCQHYR.
    PhylomeDBiQ8GVE8.

    Family and domain databases

    HAMAPiMF_00595. PEPcase_type1.
    InterProiIPR021135. PEP_COase.
    IPR018129. PEP_COase_AS.
    IPR022805. PEP_COase_bac/pln-type.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view]
    PfamiPF00311. PEPcase. 2 hits.
    [Graphical view]
    PRINTSiPR00150. PEPCARBXLASE.
    SUPFAMiSSF51621. SSF51621. 2 hits.
    PROSITEiPS00781. PEPCASE_1. 1 hit.
    PS00393. PEPCASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8GVE8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDTTDDIAE EISFQSFEDD CKLLGSLFHD VLQREVGNPF MEKVERIRIL     50
    AQSALNLRMA GIEDTANLLE KQLTSEISKM PLEEALTLAR TFTHSLNLMG 100
    IADTHHRMHK VHNVTQLARS CDDIFSQLLQ SGISPDELYK TVCKQEVEIV 150
    LTAHPTQINR RTLQYKHIRI AHLLEYNTRS DLSVEDRETL IEDLVREITS 200
    LWQTDELRRQ KPTPVDEARA GLNIVEQSLW KAVPQYLRRV SNSLKKFTGK 250
    PLPLTCTPMK FGSWMGGDRD GNPNVTAKVT KEVSLLSRWM AIDLYIREVD 300
    SLRFELSTDR CSDRFSRLAD KILEKDYDRG KSNFQKQQSS SCLPTQLPAR 350
    AHLPACIDFG ESRHTKFEIA TTDYMPPNLQ KQNEQDFSES DWEKIDNGSR 400
    SGLTSRGSFS STSQLLLQRK LFEESQVGKT SFQKLLEPPP LKRAGSAPYR 450
    IVLGEVKEKL VKTRRLLELL IEGLPCEYDP KNSYETSDQL LEPLLLCYES 500
    LQSSGARVLA DGRLADLIRR VSTFGMVLVK LDLRQEAARH SEALDAITTY 550
    LDMGTYSEWD EEKKLEFLTR ELKGKRPLVP QCIKVGPDVK EVLDTFRVAA 600
    ELGSESLGAY VISMASNASD VLAVELLQKD ARLALTSEHG KPCPGGTLRV 650
    VPLFETVNDL RAAGPSIRKL LSIDWYREHI QKNHNGHQEV MVGYSDSGKD 700
    AGRFTAAWEL YKAQENVVAA CNEFGIKITL FHGRGGSIGR GGGPTYLAIQ 750
    SQPPGSVMGS LRSTEQGEMV QAKFGIPQTA VRQLEVYTTA VLLATLKPPQ 800
    PPREEKWRNL MEEISGISCQ HYRSTVYENP EFLSYFHEAT PQAELGFLNI 850
    GSRPTRRKSS SGIGHLRAIP WVFAWTQTRF VLPAWLGVGA GLKGVSEKGH 900
    ADDLKEMYKE WPFFQSTLEL IEMVLAKADI PMTKHYDEQL VSEKRRGLGT 950
    ELRKELMTTE KYVLVISGHE KLLQDNKSLK KLIDSRLPYL NAMNMLQVEI 1000
    LKRLRRDEDN NKLRDALLIT INGIAAGMRN TG 1032
    Length:1,032
    Mass (Da):116,586
    Last modified:March 1, 2003 - v1
    Checksum:i750116B15B9ECA3E
    GO

    Sequence cautioni

    The sequence AAD49968.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence AAG52040.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ532903 mRNA. Translation: CAD58727.1.
    AC008075 Genomic DNA. Translation: AAD49968.1. Sequence problems.
    AC011914 Genomic DNA. Translation: AAG52040.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE34835.1.
    PIRiC96712.
    RefSeqiNP_177043.2. NM_105548.4.
    UniGeneiAt.45175.

    Genome annotation databases

    EnsemblPlantsiAT1G68750.1; AT1G68750.1; AT1G68750.
    GeneIDi843206.
    KEGGiath:AT1G68750.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ532903 mRNA. Translation: CAD58727.1 .
    AC008075 Genomic DNA. Translation: AAD49968.1 . Sequence problems.
    AC011914 Genomic DNA. Translation: AAG52040.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE34835.1 .
    PIRi C96712.
    RefSeqi NP_177043.2. NM_105548.4.
    UniGenei At.45175.

    3D structure databases

    ProteinModelPortali Q8GVE8.
    SMRi Q8GVE8. Positions 14-322, 446-1032.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q8GVE8.
    PRIDEi Q8GVE8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G68750.1 ; AT1G68750.1 ; AT1G68750 .
    GeneIDi 843206.
    KEGGi ath:AT1G68750.

    Organism-specific databases

    GeneFarmi 5063. 479.
    TAIRi AT1G68750.

    Phylogenomic databases

    eggNOGi COG2352.
    HOGENOMi HOG000238648.
    InParanoidi Q8GVE8.
    KOi K01595.
    OMAi ISCQHYR.
    PhylomeDBi Q8GVE8.

    Enzyme and pathway databases

    BioCyci ARA:AT1G68750-MONOMER.
    BRENDAi 4.1.1.31. 399.

    Gene expression databases

    Genevestigatori Q8GVE8.

    Family and domain databases

    HAMAPi MF_00595. PEPcase_type1.
    InterProi IPR021135. PEP_COase.
    IPR018129. PEP_COase_AS.
    IPR022805. PEP_COase_bac/pln-type.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view ]
    Pfami PF00311. PEPcase. 2 hits.
    [Graphical view ]
    PRINTSi PR00150. PEPCARBXLASE.
    SUPFAMi SSF51621. SSF51621. 2 hits.
    PROSITEi PS00781. PEPCASE_1. 1 hit.
    PS00393. PEPCASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and expression analysis of a gene encoding a bacterial-type phosphoenolpyruvate carboxylase from Arabidopsis and rice."
      Sanchez R., Cejudo F.J.
      Plant Physiol. 132:949-957(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiCAPP4_ARATH
    AccessioniPrimary (citable) accession number: Q8GVE8
    Secondary accession number(s): Q9CA39, Q9SX35
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2005
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3