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Q8GVE8 (CAPP4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxylase 4

Short name=AtPPC4
Short name=PEPC 4
Short name=PEPCase 4
EC=4.1.1.31
Gene names
Name:PPC4
Ordered Locus Names:At1g68750
ORF Names:F14K14.14, F24J5.1
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length1032 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Through the carboxylation of phosphoenolpyruvate (PEP) it forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle. HAMAP-Rule MF_00595

Catalytic activity

Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-. HAMAP-Rule MF_00595

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00595

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00595

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00595.

Tissue specificity

Expressed at low levels in flowers and siliques, and detectable in roots. Ref.1

Sequence similarities

Belongs to the PEPCase type 1 family.

Sequence caution

The sequence AAD49968.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence AAG52040.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processCarbon dioxide fixation
Photosynthesis
   Cellular componentCytoplasm
   LigandMagnesium
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbon fixation

Inferred from electronic annotation. Source: UniProtKB-KW

photosynthesis

Inferred from electronic annotation. Source: UniProtKB-KW

tricarboxylic acid cycle

Inferred from sequence or structural similarity Ref.1. Source: TAIR

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionphosphoenolpyruvate carboxylase activity

Inferred from sequence or structural similarity Ref.1. Source: TAIR

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10321032Phosphoenolpyruvate carboxylase 4 HAMAP-Rule MF_00595
PRO_0000166660

Sites

Active site1541 By similarity
Active site6991 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8GVE8 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 750116B15B9ECA3E

FASTA1,032116,586
        10         20         30         40         50         60 
MTDTTDDIAE EISFQSFEDD CKLLGSLFHD VLQREVGNPF MEKVERIRIL AQSALNLRMA 

        70         80         90        100        110        120 
GIEDTANLLE KQLTSEISKM PLEEALTLAR TFTHSLNLMG IADTHHRMHK VHNVTQLARS 

       130        140        150        160        170        180 
CDDIFSQLLQ SGISPDELYK TVCKQEVEIV LTAHPTQINR RTLQYKHIRI AHLLEYNTRS 

       190        200        210        220        230        240 
DLSVEDRETL IEDLVREITS LWQTDELRRQ KPTPVDEARA GLNIVEQSLW KAVPQYLRRV 

       250        260        270        280        290        300 
SNSLKKFTGK PLPLTCTPMK FGSWMGGDRD GNPNVTAKVT KEVSLLSRWM AIDLYIREVD 

       310        320        330        340        350        360 
SLRFELSTDR CSDRFSRLAD KILEKDYDRG KSNFQKQQSS SCLPTQLPAR AHLPACIDFG 

       370        380        390        400        410        420 
ESRHTKFEIA TTDYMPPNLQ KQNEQDFSES DWEKIDNGSR SGLTSRGSFS STSQLLLQRK 

       430        440        450        460        470        480 
LFEESQVGKT SFQKLLEPPP LKRAGSAPYR IVLGEVKEKL VKTRRLLELL IEGLPCEYDP 

       490        500        510        520        530        540 
KNSYETSDQL LEPLLLCYES LQSSGARVLA DGRLADLIRR VSTFGMVLVK LDLRQEAARH 

       550        560        570        580        590        600 
SEALDAITTY LDMGTYSEWD EEKKLEFLTR ELKGKRPLVP QCIKVGPDVK EVLDTFRVAA 

       610        620        630        640        650        660 
ELGSESLGAY VISMASNASD VLAVELLQKD ARLALTSEHG KPCPGGTLRV VPLFETVNDL 

       670        680        690        700        710        720 
RAAGPSIRKL LSIDWYREHI QKNHNGHQEV MVGYSDSGKD AGRFTAAWEL YKAQENVVAA 

       730        740        750        760        770        780 
CNEFGIKITL FHGRGGSIGR GGGPTYLAIQ SQPPGSVMGS LRSTEQGEMV QAKFGIPQTA 

       790        800        810        820        830        840 
VRQLEVYTTA VLLATLKPPQ PPREEKWRNL MEEISGISCQ HYRSTVYENP EFLSYFHEAT 

       850        860        870        880        890        900 
PQAELGFLNI GSRPTRRKSS SGIGHLRAIP WVFAWTQTRF VLPAWLGVGA GLKGVSEKGH 

       910        920        930        940        950        960 
ADDLKEMYKE WPFFQSTLEL IEMVLAKADI PMTKHYDEQL VSEKRRGLGT ELRKELMTTE 

       970        980        990       1000       1010       1020 
KYVLVISGHE KLLQDNKSLK KLIDSRLPYL NAMNMLQVEI LKRLRRDEDN NKLRDALLIT 

      1030 
INGIAAGMRN TG 

« Hide

References

« Hide 'large scale' references
[1]"Identification and expression analysis of a gene encoding a bacterial-type phosphoenolpyruvate carboxylase from Arabidopsis and rice."
Sanchez R., Cejudo F.J.
Plant Physiol. 132:949-957(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ532903 mRNA. Translation: CAD58727.1.
AC008075 Genomic DNA. Translation: AAD49968.1. Sequence problems.
AC011914 Genomic DNA. Translation: AAG52040.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE34835.1.
PIRC96712.
RefSeqNP_177043.2. NM_105548.4.
UniGeneAt.45175.

3D structure databases

ProteinModelPortalQ8GVE8.
SMRQ8GVE8. Positions 14-322, 446-1032.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ8GVE8.
PRIDEQ8GVE8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G68750.1; AT1G68750.1; AT1G68750.
GeneID843206.
KEGGath:AT1G68750.

Organism-specific databases

GeneFarm5063. 479.
TAIRAT1G68750.

Phylogenomic databases

eggNOGCOG2352.
HOGENOMHOG000238648.
InParanoidQ8GVE8.
KOK01595.
OMAFQLLNMA.
PhylomeDBQ8GVE8.
ProtClustDBCLSN2680596.

Enzyme and pathway databases

BioCycARA:AT1G68750-MONOMER.
BRENDA4.1.1.31. 399.

Gene expression databases

GenevestigatorQ8GVE8.

Family and domain databases

HAMAPMF_00595. PEPcase_type1.
InterProIPR021135. PEP_COase.
IPR018129. PEP_COase_AS.
IPR022805. PEP_COase_bac/pln-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamPF00311. PEPcase. 2 hits.
[Graphical view]
PRINTSPR00150. PEPCARBXLASE.
SUPFAMSSF51621. SSF51621. 2 hits.
PROSITEPS00781. PEPCASE_1. 1 hit.
PS00393. PEPCASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCAPP4_ARATH
AccessionPrimary (citable) accession number: Q8GVE8
Secondary accession number(s): Q9CA39, Q9SX35
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: March 1, 2003
Last modified: February 19, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names