Q8GVE8 (CAPP4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate carboxylase 4
Short name=AtPPC4
Short name=PEPC 4
Short name=PEPCase 4
EC=4.1.1.31

Gene names

Name:	PPC4
Ordered Locus Names:	At1g68750
ORF Names:	F14K14.14, F24J5.1

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1032 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Through the carboxylation of phosphoenolpyruvate (PEP) it forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.
Catalytic activity	Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.
Cofactor	Magnesium By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Expressed at low levels in flowers and siliques, and detectable in roots. Ref.1
Sequence similarities	Belongs to the PEPCase type 1 family.
Sequence caution	The sequence AAD49968.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAG52040.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Carbon dioxide fixation Photosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbon fixation Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from sequence or structural similarity Ref.1. Source: TAIR
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phosphoenolpyruvate carboxylase activity Inferred from sequence or structural similarity Ref.1. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1032	1032	Phosphoenolpyruvate carboxylase 4		PRO_0000166660
Sites
	Active site	154	1	By similarity
	Active site	699	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8GVE8 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 750116B15B9ECA3E	FASTA	1,032	116,586
	10 20 30 40 50 60 MTDTTDDIAE EISFQSFEDD CKLLGSLFHD VLQREVGNPF MEKVERIRIL AQSALNLRMA 70 80 90 100 110 120 GIEDTANLLE KQLTSEISKM PLEEALTLAR TFTHSLNLMG IADTHHRMHK VHNVTQLARS 130 140 150 160 170 180 CDDIFSQLLQ SGISPDELYK TVCKQEVEIV LTAHPTQINR RTLQYKHIRI AHLLEYNTRS 190 200 210 220 230 240 DLSVEDRETL IEDLVREITS LWQTDELRRQ KPTPVDEARA GLNIVEQSLW KAVPQYLRRV 250 260 270 280 290 300 SNSLKKFTGK PLPLTCTPMK FGSWMGGDRD GNPNVTAKVT KEVSLLSRWM AIDLYIREVD 310 320 330 340 350 360 SLRFELSTDR CSDRFSRLAD KILEKDYDRG KSNFQKQQSS SCLPTQLPAR AHLPACIDFG 370 380 390 400 410 420 ESRHTKFEIA TTDYMPPNLQ KQNEQDFSES DWEKIDNGSR SGLTSRGSFS STSQLLLQRK 430 440 450 460 470 480 LFEESQVGKT SFQKLLEPPP LKRAGSAPYR IVLGEVKEKL VKTRRLLELL IEGLPCEYDP 490 500 510 520 530 540 KNSYETSDQL LEPLLLCYES LQSSGARVLA DGRLADLIRR VSTFGMVLVK LDLRQEAARH 550 560 570 580 590 600 SEALDAITTY LDMGTYSEWD EEKKLEFLTR ELKGKRPLVP QCIKVGPDVK EVLDTFRVAA 610 620 630 640 650 660 ELGSESLGAY VISMASNASD VLAVELLQKD ARLALTSEHG KPCPGGTLRV VPLFETVNDL 670 680 690 700 710 720 RAAGPSIRKL LSIDWYREHI QKNHNGHQEV MVGYSDSGKD AGRFTAAWEL YKAQENVVAA 730 740 750 760 770 780 CNEFGIKITL FHGRGGSIGR GGGPTYLAIQ SQPPGSVMGS LRSTEQGEMV QAKFGIPQTA 790 800 810 820 830 840 VRQLEVYTTA VLLATLKPPQ PPREEKWRNL MEEISGISCQ HYRSTVYENP EFLSYFHEAT 850 860 870 880 890 900 PQAELGFLNI GSRPTRRKSS SGIGHLRAIP WVFAWTQTRF VLPAWLGVGA GLKGVSEKGH 910 920 930 940 950 960 ADDLKEMYKE WPFFQSTLEL IEMVLAKADI PMTKHYDEQL VSEKRRGLGT ELRKELMTTE 970 980 990 1000 1010 1020 KYVLVISGHE KLLQDNKSLK KLIDSRLPYL NAMNMLQVEI LKRLRRDEDN NKLRDALLIT 1030 INGIAAGMRN TG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and expression analysis of a gene encoding a bacterial-type phosphoenolpyruvate carboxylase from Arabidopsis and rice." Sanchez R., Cejudo F.J. Plant Physiol. 132:949-957(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ532903 mRNA. Translation: CAD58727.1. AC008075 Genomic DNA. Translation: AAD49968.1. Sequence problems. AC011914 Genomic DNA. Translation: AAG52040.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE34835.1.
IPI	IPI00548075.
PIR	C96712.
RefSeq	NP_177043.2. NM_105548.4.
UniGene	At.45175.
3D structure databases
ProteinModelPortal	Q8GVE8.
SMR	Q8GVE8. Positions 14-322, 442-1032.
ModBase	Search...
Proteomic databases
PaxDb	Q8GVE8.
PRIDE	Q8GVE8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G68750.1; AT1G68750.1; AT1G68750.
GeneID	843206.
KEGG	ath:AT1G68750.
Organism-specific databases
GeneFarm	5063. 479.
TAIR	At1g68750.
Phylogenomic databases
eggNOG	COG2352.
HOGENOM	HOG000238648.
InParanoid	Q8GVE8.
KO	K01595.
OMA	RENQMWT.
PhylomeDB	Q8GVE8.
ProtClustDB	CLSN2680596.
Enzyme and pathway databases
BRENDA	4.1.1.31. 399.
Gene expression databases
Genevestigator	Q8GVE8.
GermOnline	AT1G68750. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR021135. PEP_COase. IPR018129. PEP_COase_AS. IPR022805. PEP_COase_bac/pln-type. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Pfam	PF00311. PEPcase. 2 hits. [Graphical view]
PRINTS	PR00150. PEPCARBXLASE.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PROSITE	PS00781. PEPCASE_1. 1 hit. PS00393. PEPCASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAPP4_ARATH

Accession

Primary (citable) accession number: Q8GVE8
Secondary accession number(s): Q9CA39, Q9SX35

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program