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Q8GVE3 (FLRT_CITMA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Flavanone 7-O-glucoside 2''-O-beta-L-rhamnosyltransferase

EC=2.4.1.236
Alternative name(s):
1,2 rhamnosyltransferase
Gene names
Name:C12RT1
Synonyms:CM12RHAT
OrganismCitrus maxima (Pomelo) (Citrus grandis)
Taxonomic identifier37334 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsSapindalesRutaceaeCitrus

Protein attributes

Sequence length452 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the production of the bitter neohesperidosides in citrus. Shows a strict specificity for UDP-rhamnose as donor. Ref.3

Catalytic activity

UDP-L-rhamnose + a flavanone 7-O-glucoside = UDP + a flavanone 7-O-(beta-L-rhamnosyl-(1->2)-beta-D-glucoside).

Subunit structure

Monomer. Ref.3

Subcellular location

Membrane; Single-pass membrane protein Potential.

Tissue specificity

Expressed in young fruits and leaves. Ref.1

Induction

Inhibited by 10 µM UDP. Ref.3

Sequence similarities

Belongs to the UDP-glycosyltransferase family.

Biophysicochemical properties

Kinetic parameters:

KM=2.4 µM for prunin Ref.3

KM=41.5 µM for hesperetin-7-O-glucoside

KM=1.3 µM for UDP-rhamnose with prunin as substrate

KM=1.1 µM for UDP-rhamnose with hesperetin-7-O-glucoside as substrate

Ontologies

Keywords
   Cellular componentMembrane
   DomainCoiled coil
Transmembrane
Transmembrane helix
   Molecular functionGlycosyltransferase
Transferase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionflavanone 7-O-glucoside 2''-O-beta-L-rhamnosyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 452452Flavanone 7-O-glucoside 2''-O-beta-L-rhamnosyltransferase
PRO_0000310730

Regions

Transmembrane136 – 15621Helical; Potential
Coiled coil407 – 43630 Potential

Sequences

Sequence LengthMass (Da)Tools
Q8GVE3 [UniParc].

Last modified February 22, 2012. Version 2.
Checksum: C648E2E5EBBCD8CB

FASTA45251,236
        10         20         30         40         50         60 
MDTKHQDKPS ILMLPWLAHG HIAPHLELAK KLSQKNFHIY FCSTPNNLQS FGRNVEKNFS 

        70         80         90        100        110        120 
SSIQLIELQL PNTFPELPSQ NQTTKNLPPH LIYTLVGAFE DAKPAFCNIL ETLKPTLVMY 

       130        140        150        160        170        180 
DLFQPWAAEA AYQYDIAAIL FLPLSAVACS FLLHNIVNPS LKYPFFESDY QDRESKNINY 

       190        200        210        220        230        240 
FLHLTANGTL NKDRFLKAFE LSCKFVFIKT SREIESKYLD YFPSLMGNEI IPVGPLIQEP 

       250        260        270        280        290        300 
TFKEDDTKIM DWLSQKEPRS VVYASFGSEY FPSKDEIHEI ASGLLLSEVN FIWAFRLHPD 

       310        320        330        340        350        360 
EKMTIEEALP QGFAEEIERN NKGMIVQGWV PQAKILRHGS IGGFLSHCGW GSVVEGMVFG 

       370        380        390        400        410        420 
VPIIGVPMAY EQPSNAKVVV DNGMGMVVPR DKINQRLGGE EVARVIKHVV LQEEAKQIRR 

       430        440        450 
KANEISESMK KIGDAEMSVV VEKLLQLVKK SE 

« Hide

References

[1]"Citrus fruit bitter flavors: isolation and functional characterization of the gene Cm1,2RhaT encoding a 1,2 rhamnosyltransferase, a key enzyme in the biosynthesis of the bitter flavonoids of citrus."
Frydman A., Weisshaus O., Bar-Peled M., Huhman D.V., Sumner L.W., Marin F.R., Lewinsohn E., Fluhr R., Gressel J., Eyal Y.
Plant J. 40:88-100(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 136-141; 178-186; 221-228; 301-308 AND 379-392, TISSUE SPECIFICITY.
[2]Frydman A., Weisshaus O., Bar-Peled M., Lewinsohn E., Neuman H., Fluhr R., Gressel J., Eyal Y.
Submitted (JUN-2011) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]"UDP-rhamnose:flavanone-7-O-glucoside-2''-O-rhamnosyltransferase. Purification and characterization of an enzyme catalyzing the production of bitter compounds in citrus."
Bar-Peled M., Lewinsohn E., Fluhr R., Gressel J.
J. Biol. Chem. 266:20953-20959(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY048882 mRNA. Translation: AAL06646.2.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT1. Glycosyltransferase Family 1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA2.4.1.236. 1417.
SABIO-RKQ8GVE3.

Family and domain databases

InterProIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERPTHR11926. PTHR11926. 1 hit.
PfamPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEPS00375. UDPGT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFLRT_CITMA
AccessionPrimary (citable) accession number: Q8GVE3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: February 22, 2012
Last modified: February 19, 2014
This is version 49 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families