Reviewed, UniProtKB/Swiss-Prot Q8GVE3 (FLRT_CITMA)

Names and origin

Protein names

Recommended name:
    Flavanone 7-O-glucoside 2''-O-beta-L-rhamnosyltransferase
    EC=2.4.1.236
Alternative name(s):
    1,2 rhamnosyltransferase

Gene names

Name:	C12RT1
Synonyms:	CM12RHAT

Organism

Citrus maxima (Pummelo) (Citrus grandis)

Taxonomic identifier

37334 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Sapindales › Rutaceae › Citrus

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the production of the bitter neohesperidosides in citrus. Shows a strict specificity for UDP-rhamnose as donor. Ref.2
Catalytic activity	UDP-L-rhamnose + a flavanone 7-O-glucoside = UDP + a flavanone 7-O-(beta-L-rhamnosyl-(1->2)-beta-D-glucoside).
Subunit structure	Monomer. Ref.2
Subcellular location	Membrane; Single-pass membrane protein Potential.
Tissue specificity	Expressed in young fruits and leaves. Ref.1
Induction	Inhibited by 10 µM UDP. Ref.2
Sequence similarities	Belongs to the UDP-glycosyltransferase family.
biophysicochemical properties	Kinetic parameters: KM=2.4 µM for prunin KM=41.5 µM for hesperetin-7-O-glucoside KM=1.3 µM for UDP-rhamnose with prunin as substrat KM=1.1 µM for UDP-rhamnose with hesperetin-7-O-glucoside as substrat

Ontologies

Keywords
Cellular component	Membrane
Domain	Coiled coil Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	flavanone 7-O-glucoside 2''-O-beta-L-rhamnosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 452	452	Flavanone 7-O-glucoside 2''-O-beta-L-rhamnosyltransferase		PRO_0000310730
Regions
	Transmembrane	136 – 156	21	Potential
	Coiled coil	407 – 433	27	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8GVE3-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: F110C6ABD622D635	FASTA	452	51,149
	10 20 30 40 50 60 MDTKHQDKPS ILMLPWLAHG HIAPHLELAK KLSQKNFHIY FCSTPNNLQS FGRNVEKNFS 70 80 90 100 110 120 SSIQLIELQL PNTFPELPSQ NQTTKNLPPH LIYTLVGAFE DAKPAFCNIL ETLKPTLVMY 130 140 150 160 170 180 DLFQPMAAEA AYQYDIAAIL FLPLSAVACS FLLHNIVNPS LKYPFFESDY QDRESKNINY 190 200 210 220 230 240 FLHLTANGTL NKDRFLKAFE LSCKFVFIKT SREIESKYLD YFPSLMGNEI IPVGPLIQEP 250 260 270 280 290 300 TFKVDDTKIM DWLSQKEPRS VVYASFGSEY FPSTDEIHDI AIGLLLTEVN FIWAFRLHPD 310 320 330 340 350 360 EKMTIEEALP QGFAEEIERN NKGMIVQGWV PQAKILRHGS IGGFLSHCGW GSVVEGMVFG 370 380 390 400 410 420 VPIIGVPMAY EQPSNAKVVV DNGMGMVVPR DKINQRLGGE EVARVIKHVV LQEEAKQIRR 430 440 450 KANEISESMK KIGDAQMSVV VEKLLQLVKK SE

References

[1]

"Citrus fruit bitter flavors: isolation and functional characterization of the gene Cm1,2RhaT encoding a 1,2 rhamnosyltransferase, a key enzyme in the biosynthesis of the bitter flavonoids of citrus." Frydman A., Weisshaus O., Bar-Peled M., Huhman D.V., Sumner L.W., Marin F.R., Lewinsohn E., Fluhr R., Gressel J., Eyal Y. Plant J. 40:88-100(2004) [PubMed: 15361143] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 136-141; 178-186; 221-228; 301-308 AND 379-392, TISSUE SPECIFICITY.

[2]

"UDP-rhamnose:flavanone-7-O-glucoside-2''-O-rhamnosyltransferase. Purification and characterization of an enzyme catalyzing the production of bitter compounds in citrus." Bar-Peled M., Lewinsohn E., Fluhr R., Gressel J. J. Biol. Chem. 266:20953-20959(1991) [PubMed: 1939145] [Abstract] Cited for: FUNCTION, INDUCTION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY048882 mRNA. Translation: AAL06646.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Enzyme and pathway databases
BRENDA	2.4.1.236. 191704.
Family and domain databases
InterPro	IPR002213. UDP_glucos_trans. [Graphical view]
PANTHER	PTHR11926. UDP_glucos_trans. 1 hit.
Pfam	PF00201. UDPGT. 1 hit. [Graphical view]
PROSITE	PS00375. UDPGT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FLRT_CITMA

Accession

Primary (citable) accession number: Q8GVE3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	March 1, 2003
Last modified:	April 14, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)