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Q8GVE3

- FLRT_CITMA

UniProt

Q8GVE3 - FLRT_CITMA

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Protein

Flavanone 7-O-glucoside 2''-O-beta-L-rhamnosyltransferase

Gene

C12RT1

Organism
Citrus maxima (Pomelo) (Citrus grandis)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the production of the bitter neohesperidosides in citrus. Shows a strict specificity for UDP-rhamnose as donor.1 Publication

Catalytic activityi

UDP-L-rhamnose + a flavanone 7-O-glucoside = UDP + a flavanone 7-O-(beta-L-rhamnosyl-(1->2)-beta-D-glucoside).

Kineticsi

  1. KM=2.4 µM for prunin1 Publication
  2. KM=41.5 µM for hesperetin-7-O-glucoside1 Publication
  3. KM=1.3 µM for UDP-rhamnose with prunin as substrate1 Publication
  4. KM=1.1 µM for UDP-rhamnose with hesperetin-7-O-glucoside as substrate1 Publication

GO - Molecular functioni

  1. flavanone 7-O-glucoside 2''-O-beta-L-rhamnosyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.236. 1417.
SABIO-RKQ8GVE3.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Flavanone 7-O-glucoside 2''-O-beta-L-rhamnosyltransferase (EC:2.4.1.236)
Alternative name(s):
1,2 rhamnosyltransferase
Gene namesi
Name:C12RT1
Synonyms:CM12RHAT
OrganismiCitrus maxima (Pomelo) (Citrus grandis)
Taxonomic identifieri37334 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsSapindalesRutaceaeCitrus

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 452452Flavanone 7-O-glucoside 2''-O-beta-L-rhamnosyltransferasePRO_0000310730Add
BLAST

Expressioni

Tissue specificityi

Expressed in young fruits and leaves.1 Publication

Inductioni

Inhibited by 10 µM UDP.1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei136 – 15621HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili407 – 43630Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8GVE3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDTKHQDKPS ILMLPWLAHG HIAPHLELAK KLSQKNFHIY FCSTPNNLQS
60 70 80 90 100
FGRNVEKNFS SSIQLIELQL PNTFPELPSQ NQTTKNLPPH LIYTLVGAFE
110 120 130 140 150
DAKPAFCNIL ETLKPTLVMY DLFQPWAAEA AYQYDIAAIL FLPLSAVACS
160 170 180 190 200
FLLHNIVNPS LKYPFFESDY QDRESKNINY FLHLTANGTL NKDRFLKAFE
210 220 230 240 250
LSCKFVFIKT SREIESKYLD YFPSLMGNEI IPVGPLIQEP TFKEDDTKIM
260 270 280 290 300
DWLSQKEPRS VVYASFGSEY FPSKDEIHEI ASGLLLSEVN FIWAFRLHPD
310 320 330 340 350
EKMTIEEALP QGFAEEIERN NKGMIVQGWV PQAKILRHGS IGGFLSHCGW
360 370 380 390 400
GSVVEGMVFG VPIIGVPMAY EQPSNAKVVV DNGMGMVVPR DKINQRLGGE
410 420 430 440 450
EVARVIKHVV LQEEAKQIRR KANEISESMK KIGDAEMSVV VEKLLQLVKK

SE
Length:452
Mass (Da):51,236
Last modified:February 22, 2012 - v2
Checksum:iC648E2E5EBBCD8CB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY048882 mRNA. Translation: AAL06646.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY048882 mRNA. Translation: AAL06646.2 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT1. Glycosyltransferase Family 1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BRENDAi 2.4.1.236. 1417.
SABIO-RK Q8GVE3.

Family and domain databases

InterProi IPR002213. UDP_glucos_trans.
[Graphical view ]
PANTHERi PTHR11926. PTHR11926. 1 hit.
Pfami PF00201. UDPGT. 1 hit.
[Graphical view ]
PROSITEi PS00375. UDPGT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Citrus fruit bitter flavors: isolation and functional characterization of the gene Cm1,2RhaT encoding a 1,2 rhamnosyltransferase, a key enzyme in the biosynthesis of the bitter flavonoids of citrus."
    Frydman A., Weisshaus O., Bar-Peled M., Huhman D.V., Sumner L.W., Marin F.R., Lewinsohn E., Fluhr R., Gressel J., Eyal Y.
    Plant J. 40:88-100(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 136-141; 178-186; 221-228; 301-308 AND 379-392, TISSUE SPECIFICITY.
  2. Frydman A., Weisshaus O., Bar-Peled M., Lewinsohn E., Neuman H., Fluhr R., Gressel J., Eyal Y.
    Submitted (JUN-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "UDP-rhamnose:flavanone-7-O-glucoside-2''-O-rhamnosyltransferase. Purification and characterization of an enzyme catalyzing the production of bitter compounds in citrus."
    Bar-Peled M., Lewinsohn E., Fluhr R., Gressel J.
    J. Biol. Chem. 266:20953-20959(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiFLRT_CITMA
AccessioniPrimary (citable) accession number: Q8GVE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: February 22, 2012
Last modified: October 1, 2014
This is version 50 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3