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Protein

Flavanone 7-O-glucoside 2''-O-beta-L-rhamnosyltransferase

Gene

C12RT1

Organism
Citrus maxima (Pomelo) (Citrus grandis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the production of the bitter neohesperidosides in citrus. Shows a strict specificity for UDP-rhamnose as donor.1 Publication

Catalytic activityi

UDP-beta-L-rhamnose + a flavanone 7-O-beta-D-glucoside = UDP + a flavanone 7-O-(alpha-L-rhamnosyl-(1->2)-beta-D-glucoside).1 Publication

Kineticsi

  1. KM=2.4 µM for prunin1 Publication
  2. KM=41.5 µM for hesperetin-7-O-glucoside1 Publication
  3. KM=1.3 µM for UDP-rhamnose with prunin as substrate1 Publication
  4. KM=1.1 µM for UDP-rhamnose with hesperetin-7-O-glucoside as substrate1 Publication

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.4.1.236. 1410.
    SABIO-RKQ8GVE3.

    Protein family/group databases

    CAZyiGT1. Glycosyltransferase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Flavanone 7-O-glucoside 2''-O-beta-L-rhamnosyltransferase (EC:2.4.1.2361 Publication)
    Alternative name(s):
    1,2 rhamnosyltransferase
    Gene namesi
    Name:C12RT1
    Synonyms:CM12RHAT
    OrganismiCitrus maxima (Pomelo) (Citrus grandis)
    Taxonomic identifieri37334 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsSapindalesRutaceaeCitrus

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei136 – 15621HelicalSequence AnalysisAdd
    BLAST

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 452452Flavanone 7-O-glucoside 2''-O-beta-L-rhamnosyltransferasePRO_0000310730Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed in young fruits and leaves.1 Publication

    Inductioni

    Inhibited by 10 µM UDP.1 Publication

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili407 – 43630Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the UDP-glycosyltransferase family.Curated

    Keywords - Domaini

    Coiled coil, Transmembrane, Transmembrane helix

    Family and domain databases

    InterProiIPR002213. UDP_glucos_trans.
    [Graphical view]
    PANTHERiPTHR11926. PTHR11926. 1 hit.
    PfamiPF00201. UDPGT. 1 hit.
    [Graphical view]
    PROSITEiPS00375. UDPGT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8GVE3-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDTKHQDKPS ILMLPWLAHG HIAPHLELAK KLSQKNFHIY FCSTPNNLQS
    60 70 80 90 100
    FGRNVEKNFS SSIQLIELQL PNTFPELPSQ NQTTKNLPPH LIYTLVGAFE
    110 120 130 140 150
    DAKPAFCNIL ETLKPTLVMY DLFQPWAAEA AYQYDIAAIL FLPLSAVACS
    160 170 180 190 200
    FLLHNIVNPS LKYPFFESDY QDRESKNINY FLHLTANGTL NKDRFLKAFE
    210 220 230 240 250
    LSCKFVFIKT SREIESKYLD YFPSLMGNEI IPVGPLIQEP TFKEDDTKIM
    260 270 280 290 300
    DWLSQKEPRS VVYASFGSEY FPSKDEIHEI ASGLLLSEVN FIWAFRLHPD
    310 320 330 340 350
    EKMTIEEALP QGFAEEIERN NKGMIVQGWV PQAKILRHGS IGGFLSHCGW
    360 370 380 390 400
    GSVVEGMVFG VPIIGVPMAY EQPSNAKVVV DNGMGMVVPR DKINQRLGGE
    410 420 430 440 450
    EVARVIKHVV LQEEAKQIRR KANEISESMK KIGDAEMSVV VEKLLQLVKK

    SE
    Length:452
    Mass (Da):51,236
    Last modified:February 22, 2012 - v2
    Checksum:iC648E2E5EBBCD8CB
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY048882 mRNA. Translation: AAL06646.2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY048882 mRNA. Translation: AAL06646.2.

    3D structure databases

    ModBaseiSearch...
    MobiDBiSearch...

    Protein family/group databases

    CAZyiGT1. Glycosyltransferase Family 1.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Enzyme and pathway databases

    BRENDAi2.4.1.236. 1410.
    SABIO-RKQ8GVE3.

    Family and domain databases

    InterProiIPR002213. UDP_glucos_trans.
    [Graphical view]
    PANTHERiPTHR11926. PTHR11926. 1 hit.
    PfamiPF00201. UDPGT. 1 hit.
    [Graphical view]
    PROSITEiPS00375. UDPGT. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    1. "Citrus fruit bitter flavors: isolation and functional characterization of the gene Cm1,2RhaT encoding a 1,2 rhamnosyltransferase, a key enzyme in the biosynthesis of the bitter flavonoids of citrus."
      Frydman A., Weisshaus O., Bar-Peled M., Huhman D.V., Sumner L.W., Marin F.R., Lewinsohn E., Fluhr R., Gressel J., Eyal Y.
      Plant J. 40:88-100(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 136-141; 178-186; 221-228; 301-308 AND 379-392, TISSUE SPECIFICITY.
    2. Frydman A., Weisshaus O., Bar-Peled M., Lewinsohn E., Neuman H., Fluhr R., Gressel J., Eyal Y.
      Submitted (JUN-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. "UDP-rhamnose:flavanone-7-O-glucoside-2''-O-rhamnosyltransferase. Purification and characterization of an enzyme catalyzing the production of bitter compounds in citrus."
      Bar-Peled M., Lewinsohn E., Fluhr R., Gressel J.
      J. Biol. Chem. 266:20953-20959(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, INDUCTION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiFLRT_CITMA
    AccessioniPrimary (citable) accession number: Q8GVE3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 13, 2007
    Last sequence update: February 22, 2012
    Last modified: April 1, 2015
    This is version 52 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.