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Q8GVE3

- FLRT_CITMA

UniProt

Q8GVE3 - FLRT_CITMA

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Protein
Flavanone 7-O-glucoside 2''-O-beta-L-rhamnosyltransferase
Gene
C12RT1, CM12RHAT
Organism
Citrus maxima (Pomelo) (Citrus grandis)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the production of the bitter neohesperidosides in citrus. Shows a strict specificity for UDP-rhamnose as donor.1 Publication

Catalytic activityi

UDP-L-rhamnose + a flavanone 7-O-glucoside = UDP + a flavanone 7-O-(beta-L-rhamnosyl-(1->2)-beta-D-glucoside).

Kineticsi

  1. KM=2.4 µM for prunin1 Publication
  2. KM=41.5 µM for hesperetin-7-O-glucoside
  3. KM=1.3 µM for UDP-rhamnose with prunin as substrate
  4. KM=1.1 µM for UDP-rhamnose with hesperetin-7-O-glucoside as substrate

GO - Molecular functioni

  1. flavanone 7-O-glucoside 2''-O-beta-L-rhamnosyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.236. 1417.
SABIO-RKQ8GVE3.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Flavanone 7-O-glucoside 2''-O-beta-L-rhamnosyltransferase (EC:2.4.1.236)
Alternative name(s):
1,2 rhamnosyltransferase
Gene namesi
Name:C12RT1
Synonyms:CM12RHAT
OrganismiCitrus maxima (Pomelo) (Citrus grandis)
Taxonomic identifieri37334 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsSapindalesRutaceaeCitrus

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei136 – 15621Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 452452Flavanone 7-O-glucoside 2''-O-beta-L-rhamnosyltransferase
PRO_0000310730Add
BLAST

Expressioni

Tissue specificityi

Expressed in young fruits and leaves.1 Publication

Inductioni

Inhibited by 10 µM UDP.1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili407 – 43630 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8GVE3-1 [UniParc]FASTAAdd to Basket

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MDTKHQDKPS ILMLPWLAHG HIAPHLELAK KLSQKNFHIY FCSTPNNLQS    50
FGRNVEKNFS SSIQLIELQL PNTFPELPSQ NQTTKNLPPH LIYTLVGAFE 100
DAKPAFCNIL ETLKPTLVMY DLFQPWAAEA AYQYDIAAIL FLPLSAVACS 150
FLLHNIVNPS LKYPFFESDY QDRESKNINY FLHLTANGTL NKDRFLKAFE 200
LSCKFVFIKT SREIESKYLD YFPSLMGNEI IPVGPLIQEP TFKEDDTKIM 250
DWLSQKEPRS VVYASFGSEY FPSKDEIHEI ASGLLLSEVN FIWAFRLHPD 300
EKMTIEEALP QGFAEEIERN NKGMIVQGWV PQAKILRHGS IGGFLSHCGW 350
GSVVEGMVFG VPIIGVPMAY EQPSNAKVVV DNGMGMVVPR DKINQRLGGE 400
EVARVIKHVV LQEEAKQIRR KANEISESMK KIGDAEMSVV VEKLLQLVKK 450
SE 452
Length:452
Mass (Da):51,236
Last modified:February 22, 2012 - v2
Checksum:iC648E2E5EBBCD8CB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY048882 mRNA. Translation: AAL06646.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY048882 mRNA. Translation: AAL06646.2 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT1. Glycosyltransferase Family 1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BRENDAi 2.4.1.236. 1417.
SABIO-RK Q8GVE3.

Family and domain databases

InterProi IPR002213. UDP_glucos_trans.
[Graphical view ]
PANTHERi PTHR11926. PTHR11926. 1 hit.
Pfami PF00201. UDPGT. 1 hit.
[Graphical view ]
PROSITEi PS00375. UDPGT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Citrus fruit bitter flavors: isolation and functional characterization of the gene Cm1,2RhaT encoding a 1,2 rhamnosyltransferase, a key enzyme in the biosynthesis of the bitter flavonoids of citrus."
    Frydman A., Weisshaus O., Bar-Peled M., Huhman D.V., Sumner L.W., Marin F.R., Lewinsohn E., Fluhr R., Gressel J., Eyal Y.
    Plant J. 40:88-100(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 136-141; 178-186; 221-228; 301-308 AND 379-392, TISSUE SPECIFICITY.
  2. Frydman A., Weisshaus O., Bar-Peled M., Lewinsohn E., Neuman H., Fluhr R., Gressel J., Eyal Y.
    Submitted (JUN-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "UDP-rhamnose:flavanone-7-O-glucoside-2''-O-rhamnosyltransferase. Purification and characterization of an enzyme catalyzing the production of bitter compounds in citrus."
    Bar-Peled M., Lewinsohn E., Fluhr R., Gressel J.
    J. Biol. Chem. 266:20953-20959(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiFLRT_CITMA
AccessioniPrimary (citable) accession number: Q8GVE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: February 22, 2012
Last modified: February 19, 2014
This is version 49 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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