Q8GV43 (PLCD6_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoinositide phospholipase C 6
EC=3.1.4.11

Alternative name(s):
Phosphoinositide phospholipase PLC6
Short name=AtPLC6
Short name=AtPLC8
Short name=PI-PLC6

Gene names

Name:	PLC6
Synonyms:	PLC8
Ordered Locus Names:	At2g40116
ORF Names:	F27I1

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	613 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes By similarity.
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.
Cofactor	Calcium.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Tissue specificity	Expressed in leaves, flowers and siliques, but not in roots. Ref.6
Sequence similarities	Contains 1 C2 domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism
Cellular component	Cell membrane Membrane
Molecular function	Hydrolase Transducer
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phosphatidylinositol phospholipase C activity Inferred from electronic annotation. Source: EC signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 613	613	Phosphoinositide phospholipase C 6		PRO_0000324131
Regions
	Domain	137 – 281	145	PI-PLC X-box
	Domain	349 – 465	117	PI-PLC Y-box
	Domain	471 – 578	108	C2
	Compositional bias	50 – 71	22	Gly-rich
Sites
	Active site	152	1	By similarity
	Active site	198	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8GV43 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: F248C0B821903E6D	FASTA	613	70,038
	10 20 30 40 50 60 MGKEKKTESY NNDSGSYNYR MFKFYNRKFK INEVTPTDDV RDAFCQFAVG GGGGGTDGDS 70 80 90 100 110 120 SDGDGSTGVM GAEQLCSFLD DHGESTTVAE AQRLIDEVIR RRHHVTRFTR HGLDLDDFFN 130 140 150 160 170 180 FLFYDDLNPP ITPHVHQDMT APLSHYFIYT GHNSYLTGNQ LSSDCSEVPV IKALQRGVRV 190 200 210 220 230 240 IELDLWPNST GTDINVLHGR TLTTPVPLMK CLKSIRDYAF SSSPYPVIIT LEDHLTPDLQ 250 260 270 280 290 300 AKVAEMATQI FGQMLYYPES DSLLEFPSPA SLLHRIIIST KPPKEYLESR NPIVKQKDNN 310 320 330 340 350 360 VSPSSEDETP RTEEIQTLES MLFDQDFESK SDSDQEDEEA SEDQKPAYKR LITIHAGKPK 370 380 390 400 410 420 GTVKEEMKVV VDKVRRLSLS EQELDRTCSS NSQDVVRFTQ RNLLRIYPKG TRFNSSNYKP 430 440 450 460 470 480 LIGWTHGAQM IAFNMQGYGK SLWLMHGMFR ANGGCGYVKK PNFLMKKGFH DEVFDPRKKL 490 500 510 520 530 540 PVKETLKVKV YMGDGWRMDF SHTHFDAYSP PDFYTKMFIV GVPADNAKKK TKIIEDNWYP 550 560 570 580 590 600 IWDEEFSFPL TVPELALLRI EVREYDMSEK DDFGGQTCLP VAELRPGIRS VPLYDKKGEK 610 MKSVRLLMRF IFE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation." Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M. Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Arabidopsis thaliana phosphoinositide-specific phospholipase C 8 (AtPLC8)." Tasma I.M., Brendel V., Bhattacharyya M.K. Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 70-613.
[5]	"Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C." Mueller-Roeber B., Pical C. Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[6]	"Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms." Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., Gray J.E. New Phytol. 162:643-654(2004) [AGRICOLA] [Europe PMC] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002685 Genomic DNA. Translation: AEC09783.1. BX819512 mRNA. No translation available. AY150803 Genomic DNA. Translation: AAN75042.1.
IPI	IPI00523149.
RefSeq	NP_850327.2. NM_179996.2.
UniGene	At.50126.
3D structure databases
HSSP	HSSP built from PDB template 1DJH based on UniProtKB P10688.
ProteinModelPortal	Q8GV43.
SMR	Q8GV43. Positions 65-609.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT2G40116.1-P.
Proteomic databases
PaxDb	Q8GV43.
PRIDE	Q8GV43.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G40116.1; AT2G40116.1; AT2G40116.
GeneID	818602.
KEGG	ath:AT2G40116.
Organism-specific databases
TAIR	At2g40116.
Phylogenomic databases
eggNOG	NOG149692.
HOGENOM	HOG000244119.
InParanoid	Q8GV43.
KO	K05857.
OMA	VEMDEEY.
PhylomeDB	Q8GV43.
ProtClustDB	PLN02952.
Gene expression databases
Genevestigator	Q8GV43.
Family and domain databases
Gene3D	3.20.20.190. 2 hits.
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR018029. C2_membr_targeting. IPR001192. Pinositol_PLipase_C. IPR017946. PLC-like_Pdiesterase_TIM-brl. IPR015359. PLipase_C_EF-hand-like. IPR000909. PLipase_C_PInositol-sp_X_dom. IPR001711. PLipase_C_Pinositol-sp_Y. [Graphical view]
PANTHER	PTHR10336. PTHR10336. 1 hit.
Pfam	PF00168. C2. 1 hit. PF09279. efhand_like. 1 hit. PF00388. PI-PLC-X. 1 hit. PF00387. PI-PLC-Y. 1 hit. [Graphical view]
PRINTS	PR00390. PHPHLIPASEC.
SMART	SM00239. C2. 1 hit. SM00148. PLCXc. 1 hit. SM00149. PLCYc. 1 hit. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 1 hit. SSF51695. PLC-like_Pdiesterase_TIM-brl. 1 hit.
PROSITE	PS50004. C2. 1 hit. PS50007. PIPLC_X_DOMAIN. 1 hit. PS50008. PIPLC_Y_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLCD6_ARATH

Accession

Primary (citable) accession number: Q8GV43

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	May 1, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program