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Q8GV43 (PLCD6_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoinositide phospholipase C 6

EC=3.1.4.11
Alternative name(s):
Phosphoinositide phospholipase PLC6
Short name=AtPLC6
Short name=AtPLC8
Short name=PI-PLC6
Gene names
Name:PLC6
Synonyms:PLC8
Ordered Locus Names:At2g40116
ORF Names:F27I1
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length613 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes By similarity.

Catalytic activity

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactor

Calcium.

Subcellular location

Cell membrane; Peripheral membrane protein By similarity.

Tissue specificity

Expressed in leaves, flowers and siliques, but not in roots. Ref.6

Sequence similarities

Contains 1 C2 domain.

Contains 1 PI-PLC X-box domain.

Contains 1 PI-PLC Y-box domain.

Ontologies

Keywords
   Biological processLipid degradation
Lipid metabolism
   Cellular componentCell membrane
Membrane
   Molecular functionHydrolase
Transducer
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processintracellular signal transduction

Inferred from electronic annotation. Source: InterPro

lipid catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionphosphatidylinositol phospholipase C activity

Inferred from electronic annotation. Source: UniProtKB-EC

signal transducer activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 613613Phosphoinositide phospholipase C 6
PRO_0000324131

Regions

Domain137 – 281145PI-PLC X-box
Domain349 – 465117PI-PLC Y-box
Domain471 – 578108C2
Compositional bias50 – 7122Gly-rich

Sites

Active site1521 By similarity
Active site1981 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8GV43 [UniParc].

Last modified March 18, 2008. Version 2.
Checksum: F248C0B821903E6D

FASTA61370,038
        10         20         30         40         50         60 
MGKEKKTESY NNDSGSYNYR MFKFYNRKFK INEVTPTDDV RDAFCQFAVG GGGGGTDGDS 

        70         80         90        100        110        120 
SDGDGSTGVM GAEQLCSFLD DHGESTTVAE AQRLIDEVIR RRHHVTRFTR HGLDLDDFFN 

       130        140        150        160        170        180 
FLFYDDLNPP ITPHVHQDMT APLSHYFIYT GHNSYLTGNQ LSSDCSEVPV IKALQRGVRV 

       190        200        210        220        230        240 
IELDLWPNST GTDINVLHGR TLTTPVPLMK CLKSIRDYAF SSSPYPVIIT LEDHLTPDLQ 

       250        260        270        280        290        300 
AKVAEMATQI FGQMLYYPES DSLLEFPSPA SLLHRIIIST KPPKEYLESR NPIVKQKDNN 

       310        320        330        340        350        360 
VSPSSEDETP RTEEIQTLES MLFDQDFESK SDSDQEDEEA SEDQKPAYKR LITIHAGKPK 

       370        380        390        400        410        420 
GTVKEEMKVV VDKVRRLSLS EQELDRTCSS NSQDVVRFTQ RNLLRIYPKG TRFNSSNYKP 

       430        440        450        460        470        480 
LIGWTHGAQM IAFNMQGYGK SLWLMHGMFR ANGGCGYVKK PNFLMKKGFH DEVFDPRKKL 

       490        500        510        520        530        540 
PVKETLKVKV YMGDGWRMDF SHTHFDAYSP PDFYTKMFIV GVPADNAKKK TKIIEDNWYP 

       550        560        570        580        590        600 
IWDEEFSFPL TVPELALLRI EVREYDMSEK DDFGGQTCLP VAELRPGIRS VPLYDKKGEK 

       610 
MKSVRLLMRF IFE 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Arabidopsis thaliana phosphoinositide-specific phospholipase C 8 (AtPLC8)."
Tasma I.M., Brendel V., Bhattacharyya M.K.
Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 70-613.
[5]"Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
Mueller-Roeber B., Pical C.
Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[6]"Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms."
Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., Gray J.E.
New Phytol. 162:643-654(2004) [AGRICOLA] [Europe PMC]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002685 Genomic DNA. Translation: AEC09783.1.
BX819512 mRNA. No translation available.
AY150803 Genomic DNA. Translation: AAN75042.1.
RefSeqNP_850327.2. NM_179996.2.
UniGeneAt.50126.

3D structure databases

ProteinModelPortalQ8GV43.
SMRQ8GV43. Positions 65-609.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT2G40116.1-P.

Proteomic databases

PaxDbQ8GV43.
PRIDEQ8GV43.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G40116.1; AT2G40116.1; AT2G40116.
GeneID818602.
KEGGath:AT2G40116.

Organism-specific databases

TAIRAT2G40116.

Phylogenomic databases

eggNOGNOG149692.
HOGENOMHOG000244119.
InParanoidQ8GV43.
KOK05857.
OMARDYAFSS.
PhylomeDBQ8GV43.

Enzyme and pathway databases

BioCycARA:AT2G40116-MONOMER.

Gene expression databases

GenevestigatorQ8GV43.

Family and domain databases

Gene3D2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProIPR000008. C2_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERPTHR10336. PTHR10336. 1 hit.
PfamPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSPR00390. PHPHLIPASEC.
SMARTSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePLCD6_ARATH
AccessionPrimary (citable) accession number: Q8GV43
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: June 11, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names