Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8GV43

- PLCD6_ARATH

UniProt

Q8GV43 - PLCD6_ARATH

Protein

Phosphoinositide phospholipase C 6

Gene

PLC6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (18 Mar 2008)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.By similarity

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

    Cofactori

    Calcium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei152 – 1521PROSITE-ProRule annotation
    Active sitei198 – 1981PROSITE-ProRule annotation

    GO - Molecular functioni

    1. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
    2. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. lipid catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Transducer

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Enzyme and pathway databases

    BioCyciARA:AT2G40116-MONOMER.
    ReactomeiREACT_187614. Synthesis of IP3 and IP4 in the cytosol.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoinositide phospholipase C 6 (EC:3.1.4.11)
    Alternative name(s):
    Phosphoinositide phospholipase PLC6
    Short name:
    AtPLC6
    Short name:
    AtPLC8
    Short name:
    PI-PLC6
    Gene namesi
    Name:PLC6
    Synonyms:PLC8
    Ordered Locus Names:At2g40116
    ORF Names:F27I1
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G40116.

    Subcellular locationi

    Cell membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 613613Phosphoinositide phospholipase C 6PRO_0000324131Add
    BLAST

    Proteomic databases

    PaxDbiQ8GV43.
    PRIDEiQ8GV43.

    Expressioni

    Tissue specificityi

    Expressed in leaves, flowers and siliques, but not in roots.1 Publication

    Gene expression databases

    GenevestigatoriQ8GV43.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G40116.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8GV43.
    SMRiQ8GV43. Positions 65-609.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini137 – 281145PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini349 – 465117PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini471 – 578108C2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi50 – 7122Gly-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG149692.
    HOGENOMiHOG000244119.
    InParanoidiQ8GV43.
    KOiK05857.
    OMAiRDYAFSS.
    PhylomeDBiQ8GV43.

    Family and domain databases

    Gene3Di2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view]
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8GV43-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKEKKTESY NNDSGSYNYR MFKFYNRKFK INEVTPTDDV RDAFCQFAVG    50
    GGGGGTDGDS SDGDGSTGVM GAEQLCSFLD DHGESTTVAE AQRLIDEVIR 100
    RRHHVTRFTR HGLDLDDFFN FLFYDDLNPP ITPHVHQDMT APLSHYFIYT 150
    GHNSYLTGNQ LSSDCSEVPV IKALQRGVRV IELDLWPNST GTDINVLHGR 200
    TLTTPVPLMK CLKSIRDYAF SSSPYPVIIT LEDHLTPDLQ AKVAEMATQI 250
    FGQMLYYPES DSLLEFPSPA SLLHRIIIST KPPKEYLESR NPIVKQKDNN 300
    VSPSSEDETP RTEEIQTLES MLFDQDFESK SDSDQEDEEA SEDQKPAYKR 350
    LITIHAGKPK GTVKEEMKVV VDKVRRLSLS EQELDRTCSS NSQDVVRFTQ 400
    RNLLRIYPKG TRFNSSNYKP LIGWTHGAQM IAFNMQGYGK SLWLMHGMFR 450
    ANGGCGYVKK PNFLMKKGFH DEVFDPRKKL PVKETLKVKV YMGDGWRMDF 500
    SHTHFDAYSP PDFYTKMFIV GVPADNAKKK TKIIEDNWYP IWDEEFSFPL 550
    TVPELALLRI EVREYDMSEK DDFGGQTCLP VAELRPGIRS VPLYDKKGEK 600
    MKSVRLLMRF IFE 613
    Length:613
    Mass (Da):70,038
    Last modified:March 18, 2008 - v2
    Checksum:iF248C0B821903E6D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002685 Genomic DNA. Translation: AEC09783.1.
    BX819512 mRNA. No translation available.
    AY150803 Genomic DNA. Translation: AAN75042.1.
    RefSeqiNP_850327.2. NM_179996.2.
    UniGeneiAt.50126.

    Genome annotation databases

    EnsemblPlantsiAT2G40116.1; AT2G40116.1; AT2G40116.
    GeneIDi818602.
    KEGGiath:AT2G40116.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002685 Genomic DNA. Translation: AEC09783.1 .
    BX819512 mRNA. No translation available.
    AY150803 Genomic DNA. Translation: AAN75042.1 .
    RefSeqi NP_850327.2. NM_179996.2.
    UniGenei At.50126.

    3D structure databases

    ProteinModelPortali Q8GV43.
    SMRi Q8GV43. Positions 65-609.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G40116.1-P.

    Proteomic databases

    PaxDbi Q8GV43.
    PRIDEi Q8GV43.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G40116.1 ; AT2G40116.1 ; AT2G40116 .
    GeneIDi 818602.
    KEGGi ath:AT2G40116.

    Organism-specific databases

    TAIRi AT2G40116.

    Phylogenomic databases

    eggNOGi NOG149692.
    HOGENOMi HOG000244119.
    InParanoidi Q8GV43.
    KOi K05857.
    OMAi RDYAFSS.
    PhylomeDBi Q8GV43.

    Enzyme and pathway databases

    BioCyci ARA:AT2G40116-MONOMER.
    Reactomei REACT_187614. Synthesis of IP3 and IP4 in the cytosol.

    Gene expression databases

    Genevestigatori Q8GV43.

    Family and domain databases

    Gene3Di 2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view ]
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
      Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
      Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Arabidopsis thaliana phosphoinositide-specific phospholipase C 8 (AtPLC8)."
      Tasma I.M., Brendel V., Bhattacharyya M.K.
      Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 70-613.
    5. "Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
      Mueller-Roeber B., Pical C.
      Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    6. "Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms."
      Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., Gray J.E.
      New Phytol. 162:643-654(2004)
      [AGRICOLA] [Europe PMC]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiPLCD6_ARATH
    AccessioniPrimary (citable) accession number: Q8GV43
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: March 18, 2008
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3