Reviewed, UniProtKB/Swiss-Prot Q8GV43 (PLCD6_ARATH)

Names and origin

Protein names

Recommended name:
    Phosphoinositide phospholipase C 6
    EC=3.1.4.11
Alternative name(s):
    Phosphoinositide phospholipase PLC6
      Short name=PI-PLC6
      Short name=AtPLC6
      Short name=AtPLC8

Gene names

Name:	PLC6
Synonyms:	PLC8
Ordered Locus Names:	At2g40116

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	613 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes By similarity.
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.
Cofactor	Calcium.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Tissue specificity	Expressed in leaves, flowers and siliques, but not in roots. Ref.5
Sequence similarities	Contains 1 C2 domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Cell membrane Membrane
Molecular function	Hydrolase Transducer
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intracellular signaling cascade Inferred from electronic annotation. Source: InterPro lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	phosphoinositide phospholipase C activity Inferred from electronic annotation. Source: EC signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 613	613	Phosphoinositide phospholipase C 6		PRO_0000324131
Regions
	Domain	137 – 281	145	PI-PLC X-box
	Domain	349 – 465	117	PI-PLC Y-box
	Domain	471 – 578	108	C2
	Compositional bias	50 – 71	22	Gly-rich
Sites
	Active site	152	1	By similarity
	Active site	198	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8GV43-1 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: F248C0B821903E6D	FASTA	613	70,038
	10 20 30 40 50 60 MGKEKKTESY NNDSGSYNYR MFKFYNRKFK INEVTPTDDV RDAFCQFAVG GGGGGTDGDS 70 80 90 100 110 120 SDGDGSTGVM GAEQLCSFLD DHGESTTVAE AQRLIDEVIR RRHHVTRFTR HGLDLDDFFN 130 140 150 160 170 180 FLFYDDLNPP ITPHVHQDMT APLSHYFIYT GHNSYLTGNQ LSSDCSEVPV IKALQRGVRV 190 200 210 220 230 240 IELDLWPNST GTDINVLHGR TLTTPVPLMK CLKSIRDYAF SSSPYPVIIT LEDHLTPDLQ 250 260 270 280 290 300 AKVAEMATQI FGQMLYYPES DSLLEFPSPA SLLHRIIIST KPPKEYLESR NPIVKQKDNN 310 320 330 340 350 360 VSPSSEDETP RTEEIQTLES MLFDQDFESK SDSDQEDEEA SEDQKPAYKR LITIHAGKPK 370 380 390 400 410 420 GTVKEEMKVV VDKVRRLSLS EQELDRTCSS NSQDVVRFTQ RNLLRIYPKG TRFNSSNYKP 430 440 450 460 470 480 LIGWTHGAQM IAFNMQGYGK SLWLMHGMFR ANGGCGYVKK PNFLMKKGFH DEVFDPRKKL 490 500 510 520 530 540 PVKETLKVKV YMGDGWRMDF SHTHFDAYSP PDFYTKMFIV GVPADNAKKK TKIIEDNWYP 550 560 570 580 590 600 IWDEEFSFPL TVPELALLRI EVREYDMSEK DDFGGQTCLP VAELRPGIRS VPLYDKKGEK 610 MKSVRLLMRF IFE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation." Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M. Genome Res. 14:406-413(2004) [PubMed: 14993207] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Arabidopsis thaliana phosphoinositide-specific phospholipase C 8 (AtPLC8)." Tasma I.M., Brendel V., Bhattacharyya M.K. Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 70-613.
[4]	"Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C." Mueller-Roeber B., Pical C. Plant Physiol. 130:22-46(2002) [PubMed: 12226484] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[5]	"Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms." Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., Gray J.E. New Phytol. 162:643-654(2004) [Agricola: IND43668249] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC018721 Genomic DNA. No translation available. AC007658 Genomic DNA. No translation available. BX819512 mRNA. No translation available. AY150803 Genomic DNA. Translation: AAN75042.1.
IPI	IPI00523149.
RefSeq	NP_850327.2.
UniGene	At.50126
3D structure databases
HSSP	HSSP built from PDB template 1DJH based on UniProtKB P10688.
ModBase	Search...
Proteomic databases
PRIDE	Q8GV43.
Genome annotation databases
GeneID	818602.
GenomeReviews	Gene locus AT2G40116 in contig CT485783_GR.
KEGG	ath:AT2G40116.
NMPDR	fig|3702.1.peg.11140.
Organism-specific databases
TAIR	At2g40116.
Phylogenomic databases
OMA	RIEVREY.
Enzyme and pathway databases
BRENDA	3.1.4.11. 302.
Gene expression databases
Genevestigator	Q8GV43.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR018029. C2_membr_targeting. IPR015359. Phospholipase_C_EF-hand-like. IPR001192. Phospholipase_C_Pinositol-sp_C. IPR000909. Phospholipase_C_Pinositol-sp_X. IPR001711. Phospholipase_C_Pinositol-sp_Y. IPR017946. PLC-like_Pdiesterase_TIM-brl. [Graphical view]
Gene3D	G3DSA:3.20.20.190. PLC-like_Pdiesterase_TIM-brl. 1 hit.
Pfam	PF00168. C2. 1 hit. PF09279. efhand_like. 1 hit. PF00388. PI-PLC-X. 1 hit. PF00387. PI-PLC-Y. 1 hit. [Graphical view]
PRINTS	PR00390. PHPHLIPASEC.
ProDom	PD001202. PI_PLC_Y. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00239. C2. 1 hit. SM00148. PLCXc. 1 hit. SM00149. PLCYc. 1 hit. [Graphical view]
PROSITE	PS50004. C2. 1 hit. PS50007. PIPLC_X_DOMAIN. 1 hit. PS50008. PIPLC_Y_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLCD6_ARATH

Accession

Primary (citable) accession number: Q8GV43

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	November 3, 2009
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)