Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pullulanase 1, chloroplastic

Gene

PU1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in starch degradation and also probably in the trimming of pre-amylopectin chains during starch synthesis.3 Publications

Miscellaneous

Double mutant shows that PU1 and ISA3 have redundant function for starch degradation. The involvement of PU1 in amylopectin synthesis is infered from the phenotype of double mutant in PU1 and ISA2.

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in alpha- and beta-limit dextrins of amylopectin and glycogen, and in amylopectin and pullulan.

Pathwayi: starch biosynthesis

This protein is involved in the pathway starch biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway starch biosynthesis and in Glycan biosynthesis.

Pathwayi: starch degradation

This protein is involved in the pathway starch degradation, which is part of Glycan degradation.
View all proteins of this organism that are known to be involved in the pathway starch degradation and in Glycan degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei552NucleophileBy similarity1
Active sitei589Proton donorBy similarity1
Sitei721Transition state stabilizerBy similarity1

GO - Molecular functioni

  • limit dextrinase activity Source: TAIR
  • pullulanase activity Source: TAIR

GO - Biological processi

  • starch biosynthetic process Source: TAIR
  • starch catabolic process Source: TAIR

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Starch biosynthesis

Enzyme and pathway databases

BioCyciARA:AT5G04360-MONOMER
UniPathwayiUPA00152
UPA00153

Protein family/group databases

CAZyiCBM48 Carbohydrate-Binding Module Family 48
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Pullulanase 1, chloroplastic (EC:3.2.1.142)
Short name:
AtPU1
Alternative name(s):
Protein LIMIT DEXTRINASE
Short name:
AtLDA
Gene namesi
Name:PU1
Synonyms:LDA
Ordered Locus Names:At5g04360
ORF Names:T19N18.90
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G04360
TAIRilocus:2179919 AT5G04360

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

No effect on the starch level in leaves and slight increase of water-soluble polysaccharides. No alteration of the amylase-to-amylopectin ratio. ISA3 is able to fully compensate for the loss of PU1.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 62ChloroplastSequence analysisAdd BLAST62
ChainiPRO_000037953063 – 965Pullulanase 1, chloroplasticAdd BLAST903

Proteomic databases

PaxDbiQ8GTR4
PRIDEiQ8GTR4

Expressioni

Gene expression databases

ExpressionAtlasiQ8GTR4 baseline and differential
GenevisibleiQ8GTR4 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G04360.1

Structurei

3D structure databases

ProteinModelPortaliQ8GTR4
SMRiQ8GTR4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi62 – 68Poly-Ser7

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IN7N Eukaryota
COG1523 LUCA
HOGENOMiHOG000251916
InParanoidiQ8GTR4
OMAiDKIVPWY
OrthoDBiEOG093609DZ

Family and domain databases

Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR004193 Glyco_hydro_13_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR011839 Pullul_strch
IPR024561 Pullul_strch_C
PfamiView protein in Pfam
PF02922 CBM_48, 1 hit
PF11852 DUF3372, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 2 hits
TIGRFAMsiTIGR02103 pullul_strch, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8GTR4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALTLTPTSS VHLLSSISVA RPRIFAADFN LRSRWRRRRP VTSISNFRLR
60 70 80 90 100
LPSKTSLHCL CSSSSASSPM SLEVSSPNSQ FLDCLIYSRA YWVTQGVIAW
110 120 130 140 150
NVDVGEGSCY FYASKSAGLS FSEDGIDGYD LRIKLEAESG SLPADVIEKF
160 170 180 190 200
PHIRNYKSFK VPKDLDIRDL VKSQLAVVCF DAEGRLIQGT GLQLPGVLDE
210 220 230 240 250
LFSYDGPLGA HFTPEGVSLH LWAPTAQAVS VCIYKNPLDK SPMEICPLKE
260 270 280 290 300
ANGVWSTEGA CSWGGCYYVY KVSVYHPSTM KLETCYANDP YARGLSADGR
310 320 330 340 350
KTFLVNLDSD DLKPEGWDNL ADKKPCLRSF SDISIYELHV RDFSANDETV
360 370 380 390 400
EPENRGGYLA FTSKDSAGVK HLQKLVDAGL THLHLLPTFQ FGDVDDEKEN
410 420 430 440 450
WKSVDTSLLE GLRPDSTEAQ ARITEIQNDD GYNWGYNPVL WGVPKGSYAS
460 470 480 490 500
DPTGPCRIIE FRKMVQALNC TGLNVVLDVV YNHLHASGPH DKESVLDKIV
510 520 530 540 550
PGYYLRRNSD GFIENSTCVN NTASEHYMVD RLIRDDLLNW VVNYKVDGFR
560 570 580 590 600
FDLMGHIMKA TIVNAKSAIG SLRKETDGVD GSRIYLYGEG WNFGEVAENG
610 620 630 640 650
RGINASQFNL GGTGIGSFND RIRDATLGGS PFGHPLQQGF ITGLLLQPNA
660 670 680 690 700
HDHGSEATQE LMLSTAKNHI QTGMAANLKD YMLTNHEGKE VKGSEVLMHD
710 720 730 740 750
ATPVAYASLP TETINYVSAH DNETLFDIIS LKTPMEISVD ERCRINHLAS
760 770 780 790 800
SMIALSQGIP FFHAGDEILR SKSLDRDSYN SGDWFNRLDF SYSSNNWGVG
810 820 830 840 850
LPPKGKNEHN WPLIKPRLQD PSFKPKSSHI VATLHNFLDL LRIRYSSPLF
860 870 880 890 900
RLDTARAIQE RVRFHNTGPS SIPGAIVMSI EDGHRGIPSV SQIDPIYSLI
910 920 930 940 950
VVIFNARPSE FSYPSPALKD RKLELHPVQV MSADEIVKKS VYDSFSGGFT
960
VPARTTTVFV ESRNG
Length:965
Mass (Da):107,067
Last modified:May 3, 2011 - v2
Checksum:i27074C8B5D71BDAC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti859Q → K in AAO00771 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002688 Genomic DNA Translation: AED90732.1
CP002688 Genomic DNA Translation: ANM68325.1
BT002411 mRNA Translation: AAO00771.1
RefSeqiNP_001330089.1, NM_001342767.1
NP_196056.2, NM_120518.5
UniGeneiAt.26109

Genome annotation databases

EnsemblPlantsiAT5G04360.1; AT5G04360.1; AT5G04360
AT5G04360.2; AT5G04360.2; AT5G04360
GeneIDi830315
GrameneiAT5G04360.1; AT5G04360.1; AT5G04360
AT5G04360.2; AT5G04360.2; AT5G04360
KEGGiath:AT5G04360

Similar proteinsi

Entry informationi

Entry nameiPULA1_ARATH
AccessioniPrimary (citable) accession number: Q8GTR4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: May 3, 2011
Last modified: April 25, 2018
This is version 96 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health