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Q8GTR4

- PULA1_ARATH

UniProt

Q8GTR4 - PULA1_ARATH

Protein

Pullulanase 1, chloroplastic

Gene

PU1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 2 (03 May 2011)
      Previous versions | rss
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    Functioni

    Involved in starch degradation and also probably in the trimming of pre-amylopectin chains during starch synthesis.3 Publications

    Catalytic activityi

    Hydrolysis of (1->6)-alpha-D-glucosidic linkages in alpha- and beta-limit dextrins of amylopectin and glycogen, and in amylopectin and pullulan.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei552 – 5521NucleophileBy similarity
    Active sitei589 – 5891Proton donorBy similarity
    Sitei721 – 7211Transition state stabilizerBy similarity

    GO - Molecular functioni

    1. cation binding Source: InterPro
    2. limit dextrinase activity Source: TAIR
    3. pullulanase activity Source: TAIR

    GO - Biological processi

    1. starch biosynthetic process Source: TAIR
    2. starch catabolic process Source: TAIR

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Starch biosynthesis

    Enzyme and pathway databases

    BioCyciARA:AT5G04360-MONOMER.
    UniPathwayiUPA00152.
    UPA00153.

    Protein family/group databases

    CAZyiCBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pullulanase 1, chloroplastic (EC:3.2.1.142)
    Short name:
    AtPU1
    Alternative name(s):
    Protein LIMIT DEXTRINASE
    Short name:
    AtLDA
    Gene namesi
    Name:PU1
    Synonyms:LDA
    Ordered Locus Names:At5g04360
    ORF Names:T19N18.90
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G04360.

    Subcellular locationi

    Plastidchloroplast stroma 2 Publications

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast stroma Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    Pathology & Biotechi

    Disruption phenotypei

    No effect on the starch level in leaves and slight increase of water-soluble polysaccharides. No alteration of the amylase-to-amylopectin ratio. ISA3 is able to fully compensate for the loss of PU1.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 6262ChloroplastSequence AnalysisAdd
    BLAST
    Chaini63 – 965903Pullulanase 1, chloroplasticPRO_0000379530Add
    BLAST

    Proteomic databases

    PaxDbiQ8GTR4.
    PRIDEiQ8GTR4.

    Expressioni

    Gene expression databases

    GenevestigatoriQ8GTR4.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT5G04360.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8GTR4.
    SMRiQ8GTR4. Positions 88-963.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi62 – 687Poly-Ser

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1523.
    HOGENOMiHOG000251916.
    InParanoidiQ8GTR4.
    OMAiERCRINH.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    3.20.20.80. 3 hits.
    InterProiIPR015902. Glyco_hydro_13.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR011839. Pullul_strch.
    IPR024561. Pullul_strch_C.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF02922. CBM_48. 1 hit.
    PF11852. DUF3372. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 2 hits.
    TIGRFAMsiTIGR02103. pullul_strch. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8GTR4-1 [UniParc]FASTAAdd to Basket

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    MALTLTPTSS VHLLSSISVA RPRIFAADFN LRSRWRRRRP VTSISNFRLR    50
    LPSKTSLHCL CSSSSASSPM SLEVSSPNSQ FLDCLIYSRA YWVTQGVIAW 100
    NVDVGEGSCY FYASKSAGLS FSEDGIDGYD LRIKLEAESG SLPADVIEKF 150
    PHIRNYKSFK VPKDLDIRDL VKSQLAVVCF DAEGRLIQGT GLQLPGVLDE 200
    LFSYDGPLGA HFTPEGVSLH LWAPTAQAVS VCIYKNPLDK SPMEICPLKE 250
    ANGVWSTEGA CSWGGCYYVY KVSVYHPSTM KLETCYANDP YARGLSADGR 300
    KTFLVNLDSD DLKPEGWDNL ADKKPCLRSF SDISIYELHV RDFSANDETV 350
    EPENRGGYLA FTSKDSAGVK HLQKLVDAGL THLHLLPTFQ FGDVDDEKEN 400
    WKSVDTSLLE GLRPDSTEAQ ARITEIQNDD GYNWGYNPVL WGVPKGSYAS 450
    DPTGPCRIIE FRKMVQALNC TGLNVVLDVV YNHLHASGPH DKESVLDKIV 500
    PGYYLRRNSD GFIENSTCVN NTASEHYMVD RLIRDDLLNW VVNYKVDGFR 550
    FDLMGHIMKA TIVNAKSAIG SLRKETDGVD GSRIYLYGEG WNFGEVAENG 600
    RGINASQFNL GGTGIGSFND RIRDATLGGS PFGHPLQQGF ITGLLLQPNA 650
    HDHGSEATQE LMLSTAKNHI QTGMAANLKD YMLTNHEGKE VKGSEVLMHD 700
    ATPVAYASLP TETINYVSAH DNETLFDIIS LKTPMEISVD ERCRINHLAS 750
    SMIALSQGIP FFHAGDEILR SKSLDRDSYN SGDWFNRLDF SYSSNNWGVG 800
    LPPKGKNEHN WPLIKPRLQD PSFKPKSSHI VATLHNFLDL LRIRYSSPLF 850
    RLDTARAIQE RVRFHNTGPS SIPGAIVMSI EDGHRGIPSV SQIDPIYSLI 900
    VVIFNARPSE FSYPSPALKD RKLELHPVQV MSADEIVKKS VYDSFSGGFT 950
    VPARTTTVFV ESRNG 965
    Length:965
    Mass (Da):107,067
    Last modified:May 3, 2011 - v2
    Checksum:i27074C8B5D71BDAC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti859 – 8591Q → K in AAO00771. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002688 Genomic DNA. Translation: AED90732.1.
    BT002411 mRNA. Translation: AAO00771.1.
    RefSeqiNP_196056.2. NM_120518.4.
    UniGeneiAt.26109.

    Genome annotation databases

    EnsemblPlantsiAT5G04360.1; AT5G04360.1; AT5G04360.
    GeneIDi830315.
    KEGGiath:AT5G04360.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002688 Genomic DNA. Translation: AED90732.1 .
    BT002411 mRNA. Translation: AAO00771.1 .
    RefSeqi NP_196056.2. NM_120518.4.
    UniGenei At.26109.

    3D structure databases

    ProteinModelPortali Q8GTR4.
    SMRi Q8GTR4. Positions 88-963.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT5G04360.1-P.

    Protein family/group databases

    CAZyi CBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Proteomic databases

    PaxDbi Q8GTR4.
    PRIDEi Q8GTR4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G04360.1 ; AT5G04360.1 ; AT5G04360 .
    GeneIDi 830315.
    KEGGi ath:AT5G04360.

    Organism-specific databases

    TAIRi AT5G04360.

    Phylogenomic databases

    eggNOGi COG1523.
    HOGENOMi HOG000251916.
    InParanoidi Q8GTR4.
    OMAi ERCRINH.

    Enzyme and pathway databases

    UniPathwayi UPA00152 .
    UPA00153 .
    BioCyci ARA:AT5G04360-MONOMER.

    Gene expression databases

    Genevestigatori Q8GTR4.

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    3.20.20.80. 3 hits.
    InterProi IPR015902. Glyco_hydro_13.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR011839. Pullul_strch.
    IPR024561. Pullul_strch_C.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF02922. CBM_48. 1 hit.
    PF11852. DUF3372. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 2 hits.
    TIGRFAMsi TIGR02103. pullul_strch. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Mutants of Arabidopsis lacking a chloroplastic isoamylase accumulate phytoglycogen and an abnormal form of amylopectin."
      Wattebled F., Dong Y., Dumez S., Delvalle D., Planchot V., Berbezy P., Vyas D., Colonna P., Chatterjee M., Ball S., D'Hulst C.
      Plant Physiol. 138:184-195(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    5. "Evidence for distinct mechanisms of starch granule breakdown in plants."
      Delatte T., Umhang M., Trevisan M., Eicke S., Thorneycroft D., Smith S.M., Zeeman S.C.
      J. Biol. Chem. 281:12050-12059(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    6. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
      Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
      PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. "Starch granule biosynthesis in Arabidopsis is abolished by removal of all debranching enzymes but restored by the subsequent removal of an endoamylase."
      Streb S., Delatte T., Umhang M., Eicke S., Schorderet M., Reinhardt D., Zeeman S.C.
      Plant Cell 20:3448-3466(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Further evidence for the mandatory nature of polysaccharide debranching for the aggregation of semicrystalline starch and for overlapping functions of debranching enzymes in Arabidopsis leaves."
      Wattebled F., Planchot V., Dong Y., Szydlowski N., Pontoire B., Devin A., Ball S., D'Hulst C.
      Plant Physiol. 148:1309-1323(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiPULA1_ARATH
    AccessioniPrimary (citable) accession number: Q8GTR4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 7, 2009
    Last sequence update: May 3, 2011
    Last modified: October 1, 2014
    This is version 75 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Double mutant shows that PU1 and ISA3 have redundant function for starch degradation. The involvement of PU1 in amylopectin synthesis is infered from the phenotype of double mutant in PU1 and ISA2.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3