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Protein

Flower-specific defensin

Gene

D1

Organism
Nicotiana alata (Winged tobacco) (Persian tobacco)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plant defense peptide with antifungal activity against F.oxysporum and B.cinerea. Retards the growth of the Lepidopteran insect pests H.armigera and H.punctigera.

pH dependencei

Stable under extremes of pH.

Temperature dependencei

Stable under extremes of temperature.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Fungicide

Keywords - Biological processi

Plant defense

Names & Taxonomyi

Protein namesi
Recommended name:
Flower-specific defensin
Alternative name(s):
NaD1
Gene namesi
Name:D1
OrganismiNicotiana alata (Winged tobacco) (Persian tobacco)
Taxonomic identifieri4087 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 251 PublicationAdd BLAST25
ChainiPRO_000000704226 – 72Flower-specific defensinAdd BLAST47
PropeptideiPRO_000000704373 – 105Removed in mature formAdd BLAST33

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi28 ↔ 72
Disulfide bondi39 ↔ 59
Disulfide bondi45 ↔ 66
Disulfide bondi49 ↔ 68

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Most abundant in the epidermal cell layers of the petals and sepals, within the connective cells of the anthers, and the cortical cells of the style. Not detected in the tapetum, pollen mother cells, the transmitting tissue, the vascular bundles of the anther and style or in leaves. Expressed also in ovaries, but barley detectable in roots.

Structurei

Secondary structure

1105
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi27 – 31Combined sources5
Helixi42 – 51Combined sources10
Beta strandi55 – 59Combined sources5
Beta strandi61 – 63Combined sources3
Beta strandi66 – 71Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AAZX-ray1.40A/B26-72[»]
4AB0X-ray1.64A/B26-72[»]
4CQKX-ray1.60A/B/C/D/E/F/G/H/I/J/K/L/M/N26-72[»]
ProteinModelPortaliQ8GTM0.
SMRiQ8GTM0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DEFL family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR008176. Defensin_plant.
IPR003614. Scorpion_toxin-like.
[Graphical view]
PRINTSiPR00288. PUROTHIONIN.
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS00940. GAMMA_THIONIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8GTM0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARSLCFMAF AILAMMLFVA YEVQARECKT ESNTFPGICI TKPPCRKACI
60 70 80 90 100
SEKFTDGHCS KILRRCLCTK PCVFDEKMTK TGAEILAEEA KTLAAALLEE

EIMDN
Length:105
Mass (Da):11,722
Last modified:March 1, 2003 - v1
Checksum:iDA7F41736CEE6AC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF509566 mRNA. Translation: AAN70999.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF509566 mRNA. Translation: AAN70999.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AAZX-ray1.40A/B26-72[»]
4AB0X-ray1.64A/B26-72[»]
4CQKX-ray1.60A/B/C/D/E/F/G/H/I/J/K/L/M/N26-72[»]
ProteinModelPortaliQ8GTM0.
SMRiQ8GTM0.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR008176. Defensin_plant.
IPR003614. Scorpion_toxin-like.
[Graphical view]
PRINTSiPR00288. PUROTHIONIN.
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS00940. GAMMA_THIONIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDEF_NICAL
AccessioniPrimary (citable) accession number: Q8GTM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.