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Protein

Malonyl-coenzyme A:anthocyanin 3-O-glucoside-6''-O-malonyltransferase

Gene

3MAT

Organism
Dahlia pinnata (Pinnate dahlia) (Dahlia variabilis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of the malonyl group from malonyl-CoA to pelargonidin 3-O-glucoside to produce pelargonidin 3-O-6''-O-malonylglucoside. Can also transfer the malonyl group from malonyl-CoA to cyanidin 3-O-glucoside, delphinidin 3-O-glucoside and quercetin 3-O-glucoside.1 Publication

Catalytic activityi

Malonyl-CoA + an anthocyanidin 3-O-beta-D-glucoside = CoA + an anthocyanidin 3-O-(6-O-malonyl-beta-D-glucoside).1 Publication

Enzyme regulationi

Completely inhibited by 5 mM N-ethylmaleimide or 0.1 mM Cu2+. Partially inhibited by 0.1 mM Fe2+ or 0.1 mM Hg2+.1 Publication

Kineticsi

  1. KM=18.8 µM for malonyl-CoA1 Publication
  2. KM=46.7 µM for pelargonidin1 Publication

    pH dependencei

    Optimum pH is 8.5. Active over the pH range 5.5-11.0. Stable from pH 6.5 to 7.5 for 20 hours at 20 degrees Celsius.1 Publication

    Temperature dependencei

    Stable below 45 degrees Celsius for 20 minutes at pH 7.0.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei173Proton acceptorSequence analysis1
    Active sitei400Proton acceptorSequence analysis1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionAcyltransferase, Transferase
    Biological processPhenylpropanoid metabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-12015.
    BRENDAi2.3.1.171. 1828.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malonyl-coenzyme A:anthocyanin 3-O-glucoside-6''-O-malonyltransferase1 Publication (EC:2.3.1.171)
    Short name:
    Dv3MaT1 Publication
    Short name:
    Malonyl CoA:anthocyanin 3-O-glucoside-6''-O-malonyltransferaseImported
    Gene namesi
    Name:3MAT1 Publication
    OrganismiDahlia pinnata (Pinnate dahlia) (Dahlia variabilis)
    Taxonomic identifieri101596 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesAsteraceaeAsteroideaeHeliantheae allianceCoreopsideaeDahlia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004051261 – 460Malonyl-coenzyme A:anthocyanin 3-O-glucoside-6''-O-malonyltransferaseAdd BLAST460

    Expressioni

    Developmental stagei

    Expression is highest in petals at stage 2 of flower development (when the top of the bud is open, flowers are 6-9 mm in length and almost pigmented).1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ8GSN8.
    SMRiQ8GSN8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the plant acyltransferase family.Sequence analysis

    Phylogenomic databases

    KOiK12931.

    Family and domain databases

    InterProiView protein in InterPro
    IPR003480. Transferase.
    PfamiView protein in Pfam
    PF02458. Transferase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8GSN8-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDNIPNLTIL EHSRISPPPS TIGHRSLPLT FFDIAWLLFP PVHHLYFYHF
    60 70 80 90 100
    PYSKSHFTET VIPNLKHSLS ITLQHYFPFV GKLIVYPNPH DSTRKPEIRH
    110 120 130 140 150
    VEGDSVALTF AETTLDFNDL SANHPRKCEN FYPLVPPLGN AVKESDYVTL
    160 170 180 190 200
    PVFSVQVTYF PNSGISIGLT NHHSLSDANT RFGFLKAWAS VCETGEDQPF
    210 220 230 240 250
    LKNGSPPVFD RVVVNPQLYE NRLNQTRLGT FYQAPSLVGS SSDRVRATFV
    260 270 280 290 300
    LARTHISGLK KQVLTQLPML EYTSSFTVTC GYIWSCIVKS LVNMGEKKGE
    310 320 330 340 350
    DELEQFIVSV GCRSRLDPPL PENYFGNCSA PCIVTIKNGV LKGENGFVMA
    360 370 380 390 400
    AKLIGEGISK MVNKKGGILE YADRWYDGFK IPARKMGISG TPKLNFYDID
    410 420 430 440 450
    FGWGKAMKYE VVSIDYSASV SLSACKESAQ DFEIGVCFPS MQMEAFGKIF
    460
    NDGLESAIAS
    Length:460
    Mass (Da):51,302
    Last modified:March 1, 2003 - v1
    Checksum:i421F2B012B3B020E
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF489108 mRNA. Translation: AAO12206.1.

    Genome annotation databases

    KEGGiag:AAO12206.

    Similar proteinsi

    Entry informationi

    Entry namei3MAT_DAHPI
    AccessioniPrimary (citable) accession number: Q8GSN8
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 8, 2011
    Last sequence update: March 1, 2003
    Last modified: May 10, 2017
    This is version 38 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families