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Q8GSM3

- LOX22_HORVU

UniProt

Q8GSM3 - LOX22_HORVU

Protein

Lipoxygenase 2.2, chloroplastic

Gene

LOX2.2

Organism
Hordeum vulgare (Barley)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. This enzyme exhibits linoleate 13-lipoxygenase activity.

    Catalytic activityi

    Linoleate + O2 = (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate.
    Alpha-linolenate + O2 = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate.

    Cofactori

    Binds 1 iron ion per subunit. Iron is tightly bound By similarity.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi588 – 5881Iron; catalyticPROSITE-ProRule annotation
    Metal bindingi593 – 5931Iron; catalyticPROSITE-ProRule annotation
    Metal bindingi778 – 7781Iron; catalyticPROSITE-ProRule annotation
    Metal bindingi782 – 7821Iron; catalyticPROSITE-ProRule annotation
    Metal bindingi932 – 9321Iron; via carboxylate; catalyticPROSITE-ProRule annotation

    GO - Molecular functioni

    1. iron ion binding Source: InterPro
    2. linoleate 13S-lipoxygenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. oxylipin biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Dioxygenase, Oxidoreductase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00382.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoxygenase 2.2, chloroplastic (EC:1.13.11.12)
    Alternative name(s):
    LOX2:Hv:2
    Gene namesi
    Name:LOX2.2
    OrganismiHordeum vulgare (Barley)
    Taxonomic identifieri4513 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

    Organism-specific databases

    GrameneiQ8GSM3.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 932Lipoxygenase 2.2, chloroplasticPRO_0000018324
    Transit peptidei1 – ?ChloroplastSequence Analysis

    Expressioni

    Gene expression databases

    GenevestigatoriQ8GSM3.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8GSM3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini79 – 219141PLATPROSITE-ProRule annotationAdd
    BLAST
    Domaini223 – 932710LipoxygenasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the lipoxygenase family.Curated
    Contains 1 lipoxygenase domain.PROSITE-ProRule annotation
    Contains 1 PLAT domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di2.60.60.20. 1 hit.
    4.10.372.10. 1 hit.
    InterProiIPR008976. Lipase_LipOase.
    IPR000907. LipOase.
    IPR013819. LipOase_C.
    IPR020834. LipOase_CS.
    IPR020833. LipOase_Fe_BS.
    IPR001246. LipOase_pln.
    IPR027433. Lipoxygenase_domain_3.
    IPR001024. PLAT/LH2_dom.
    [Graphical view]
    PANTHERiPTHR11771. PTHR11771. 1 hit.
    PfamiPF00305. Lipoxygenase. 1 hit.
    PF01477. PLAT. 1 hit.
    [Graphical view]
    PRINTSiPR00087. LIPOXYGENASE.
    PR00468. PLTLPOXGNASE.
    SMARTiSM00308. LH2. 1 hit.
    [Graphical view]
    SUPFAMiSSF48484. SSF48484. 1 hit.
    SSF49723. SSF49723. 1 hit.
    PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
    PS00081. LIPOXYGENASE_2. 1 hit.
    PS51393. LIPOXYGENASE_3. 1 hit.
    PS50095. PLAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8GSM3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQTATKPLVG ARAVPLSRRA SFLVAEARRK PSTNARRTRV GSTSTTTTTT    50
    TILTDVNGPA LTTVAKPGHQ YDLKQTVEMK ATVSVHMKSF WWSDEKKERA 100
    RDWAYDLILG SWLTLELVSS ELDPKTGQEH DVISGKLKHS RETEKDYDLY 150
    EAIFTCRHRL APSGAVRLVN YHHTEMLLGE VKIFPAGEDP TKSSAVTLFH 200
    CQSWIDPSHC SPDKRTFFPV EKSYIPSQTP KGVEKLRKSE LEALRGNGCG 250
    ERKKHDRIYD YDVYNDLGKP ESKRPVLGGK EHPYPRRCRT GRPRSKTDPS 300
    SEEESHKKGE MYVPRDETFT ERKEQAFLTK QLLSQLHGLC TGLKVNKDIL 350
    PSFPTLASID ALYDDDFRNQ PVQPEGGKVR LILDLLAKEL VHLVKLEGAE 400
    FVEGIRRVFK FETPEIHDMD KLAWFRDEEF ARQTLAGMNP LSIQLVTELP 450
    IVSKLDELKY GPADSLITKE LIEKQINRIM TAEEAVAQKK LFMLDYHDLL 500
    LPYVHRVRKL DNKTMYGSRT LFFLADDGTL RPIAIELTRP KSPHKQQWRK 550
    VFTPGSGYSG SVTGSWEWQL AKIHVLSHDT GYHQLVSHWL RTHCCVEPYV 600
    IAANRQLSQM HPIYRLLHPH FRFTMEINAQ ARGMLICADG IIEKTFSPGE 650
    FSMEISSAAY DKQWRFDMEA LPEDLIRRGM AVRGEDGKLE LAIEDYPYAN 700
    DGLLVWDAIK QWASDYVAHY YPCAVDIVDD EELQDWWTEV RTKGHPDKQD 750
    EPWWPELDCH ESLVQVLATI MWVTSAHHAA VNFGQYPMAG YVPNHPSIAR 800
    RNMPCEMGPE EMLAFKAAPE KVWLDTLPSQ LQTVMVMATL DLLSSHASDE 850
    EYMGTHQEPA WQRDGEVDKA FQVFQKKMRD IAEQVEEWNK DDSRRNRHGA 900
    GVVPYVLLRP LNGNPMDAKT VMEMGIPNSI SI 932
    Length:932
    Mass (Da):106,493
    Last modified:March 1, 2003 - v1
    Checksum:i64D80DF4370F30E0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ507212 mRNA. Translation: CAD45186.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ507212 mRNA. Translation: CAD45186.1 .

    3D structure databases

    ProteinModelPortali Q8GSM3.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    Gramenei Q8GSM3.

    Enzyme and pathway databases

    UniPathwayi UPA00382 .

    Gene expression databases

    Genevestigatori Q8GSM3.

    Family and domain databases

    Gene3Di 2.60.60.20. 1 hit.
    4.10.372.10. 1 hit.
    InterProi IPR008976. Lipase_LipOase.
    IPR000907. LipOase.
    IPR013819. LipOase_C.
    IPR020834. LipOase_CS.
    IPR020833. LipOase_Fe_BS.
    IPR001246. LipOase_pln.
    IPR027433. Lipoxygenase_domain_3.
    IPR001024. PLAT/LH2_dom.
    [Graphical view ]
    PANTHERi PTHR11771. PTHR11771. 1 hit.
    Pfami PF00305. Lipoxygenase. 1 hit.
    PF01477. PLAT. 1 hit.
    [Graphical view ]
    PRINTSi PR00087. LIPOXYGENASE.
    PR00468. PLTLPOXGNASE.
    SMARTi SM00308. LH2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48484. SSF48484. 1 hit.
    SSF49723. SSF49723. 1 hit.
    PROSITEi PS00711. LIPOXYGENASE_1. 1 hit.
    PS00081. LIPOXYGENASE_2. 1 hit.
    PS51393. LIPOXYGENASE_3. 1 hit.
    PS50095. PLAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Jasmonate-induced lipid peroxidation in barley leaves initiated by distinct 13-LOX forms of the chloroplast."
      Bachmann A., Hause B., Maucher H., Garbe E., Voeroes K., Weichert H., Wasternack C., Feussner I.
      Biol. Chem. 383:1645-1657(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
      Strain: cv. Salome.
      Tissue: Leaf.

    Entry informationi

    Entry nameiLOX22_HORVU
    AccessioniPrimary (citable) accession number: Q8GSM3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2003
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3