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Q8GSM3 (LOX22_HORVU) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lipoxygenase 2.2, chloroplastic

EC=1.13.11.12
Alternative name(s):
LOX2:Hv:2
Gene names
Name:LOX2.2
OrganismHordeum vulgare (Barley)
Taxonomic identifier4513 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Protein attributes

Sequence length932 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. This enzyme exhibits linoleate 13-lipoxygenase activity.

Catalytic activity

Linoleate + O2 = (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate.

Alpha-linolenate + O2 = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate.

Cofactor

Binds 1 iron ion per subunit. Iron is tightly bound By similarity.

Pathway

Lipid metabolism; oxylipin biosynthesis.

Subcellular location

Plastidchloroplast.

Sequence similarities

Belongs to the lipoxygenase family.

Contains 1 lipoxygenase domain.

Contains 1 PLAT domain.

Ontologies

Keywords
   Biological processFatty acid biosynthesis
Fatty acid metabolism
Lipid biosynthesis
Lipid metabolism
Oxylipin biosynthesis
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandIron
Metal-binding
   Molecular functionDioxygenase
Oxidoreductase
Gene Ontology (GO)
   Biological_processoxylipin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioniron ion binding

Inferred from electronic annotation. Source: InterPro

linoleate 13S-lipoxygenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Chloroplast Potential
Chain? – 932Lipoxygenase 2.2, chloroplasticPRO_0000018324

Regions

Domain79 – 219141PLAT
Domain223 – 932710Lipoxygenase

Sites

Metal binding5881Iron; catalytic By similarity
Metal binding5931Iron; catalytic By similarity
Metal binding7781Iron; catalytic By similarity
Metal binding7821Iron; catalytic By similarity
Metal binding9321Iron; via carboxylate; catalytic By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8GSM3 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 64D80DF4370F30E0

FASTA932106,493
        10         20         30         40         50         60 
MQTATKPLVG ARAVPLSRRA SFLVAEARRK PSTNARRTRV GSTSTTTTTT TILTDVNGPA 

        70         80         90        100        110        120 
LTTVAKPGHQ YDLKQTVEMK ATVSVHMKSF WWSDEKKERA RDWAYDLILG SWLTLELVSS 

       130        140        150        160        170        180 
ELDPKTGQEH DVISGKLKHS RETEKDYDLY EAIFTCRHRL APSGAVRLVN YHHTEMLLGE 

       190        200        210        220        230        240 
VKIFPAGEDP TKSSAVTLFH CQSWIDPSHC SPDKRTFFPV EKSYIPSQTP KGVEKLRKSE 

       250        260        270        280        290        300 
LEALRGNGCG ERKKHDRIYD YDVYNDLGKP ESKRPVLGGK EHPYPRRCRT GRPRSKTDPS 

       310        320        330        340        350        360 
SEEESHKKGE MYVPRDETFT ERKEQAFLTK QLLSQLHGLC TGLKVNKDIL PSFPTLASID 

       370        380        390        400        410        420 
ALYDDDFRNQ PVQPEGGKVR LILDLLAKEL VHLVKLEGAE FVEGIRRVFK FETPEIHDMD 

       430        440        450        460        470        480 
KLAWFRDEEF ARQTLAGMNP LSIQLVTELP IVSKLDELKY GPADSLITKE LIEKQINRIM 

       490        500        510        520        530        540 
TAEEAVAQKK LFMLDYHDLL LPYVHRVRKL DNKTMYGSRT LFFLADDGTL RPIAIELTRP 

       550        560        570        580        590        600 
KSPHKQQWRK VFTPGSGYSG SVTGSWEWQL AKIHVLSHDT GYHQLVSHWL RTHCCVEPYV 

       610        620        630        640        650        660 
IAANRQLSQM HPIYRLLHPH FRFTMEINAQ ARGMLICADG IIEKTFSPGE FSMEISSAAY 

       670        680        690        700        710        720 
DKQWRFDMEA LPEDLIRRGM AVRGEDGKLE LAIEDYPYAN DGLLVWDAIK QWASDYVAHY 

       730        740        750        760        770        780 
YPCAVDIVDD EELQDWWTEV RTKGHPDKQD EPWWPELDCH ESLVQVLATI MWVTSAHHAA 

       790        800        810        820        830        840 
VNFGQYPMAG YVPNHPSIAR RNMPCEMGPE EMLAFKAAPE KVWLDTLPSQ LQTVMVMATL 

       850        860        870        880        890        900 
DLLSSHASDE EYMGTHQEPA WQRDGEVDKA FQVFQKKMRD IAEQVEEWNK DDSRRNRHGA 

       910        920        930 
GVVPYVLLRP LNGNPMDAKT VMEMGIPNSI SI 

« Hide

References

[1]"Jasmonate-induced lipid peroxidation in barley leaves initiated by distinct 13-LOX forms of the chloroplast."
Bachmann A., Hause B., Maucher H., Garbe E., Voeroes K., Weichert H., Wasternack C., Feussner I.
Biol. Chem. 383:1645-1657(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
Strain: cv. Salome.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ507212 mRNA. Translation: CAD45186.1.

3D structure databases

ProteinModelPortalQ8GSM3.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneQ8GSM3.

Enzyme and pathway databases

UniPathwayUPA00382.

Gene expression databases

GenevestigatorQ8GSM3.

Family and domain databases

Gene3D2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProIPR008976. Lipase_LipOase.
IPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_pln.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERPTHR11771. PTHR11771. 1 hit.
PfamPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLOX22_HORVU
AccessionPrimary (citable) accession number: Q8GSM3
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: March 1, 2003
Last modified: October 16, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways