Reviewed, UniProtKB/Swiss-Prot Q8GSM3 (LOX22_HORVU)

Names and origin

Protein names

Recommended name:
    Lipoxygenase 2.2, chloroplastic
    EC=1.13.11.12
Alternative name(s):
    LOX2:Hv:2

Gene names

Name:

LOX2.2

Organism

Hordeum vulgare (Barley)

Taxonomic identifier

4513 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	932 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. This enzyme exhibits linoleate 13-lipoxygenase activity.
Catalytic activity	Linoleate + O2 = (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoate.
Cofactor	Binds 1 iron ion per subunit. Iron is tightly bound By similarity.
Pathway	Lipid metabolism; oxylipin biosynthesis.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis Oxylipin biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW oxylipin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW lipoxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 932		Lipoxygenase 2.2, chloroplastic		PRO_0000018324
Regions
	Domain	79 – 219	141	PLAT
	Domain	223 – 932	710	Lipoxygenase
Sites
	Metal binding	588	1	Iron; catalytic By similarity
	Metal binding	593	1	Iron; catalytic By similarity
	Metal binding	778	1	Iron; catalytic By similarity
	Metal binding	782	1	Iron; catalytic By similarity
	Metal binding	932	1	Iron; via carboxylate; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8GSM3-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 64D80DF4370F30E0	FASTA	932	106,493
	10 20 30 40 50 60 MQTATKPLVG ARAVPLSRRA SFLVAEARRK PSTNARRTRV GSTSTTTTTT TILTDVNGPA 70 80 90 100 110 120 LTTVAKPGHQ YDLKQTVEMK ATVSVHMKSF WWSDEKKERA RDWAYDLILG SWLTLELVSS 130 140 150 160 170 180 ELDPKTGQEH DVISGKLKHS RETEKDYDLY EAIFTCRHRL APSGAVRLVN YHHTEMLLGE 190 200 210 220 230 240 VKIFPAGEDP TKSSAVTLFH CQSWIDPSHC SPDKRTFFPV EKSYIPSQTP KGVEKLRKSE 250 260 270 280 290 300 LEALRGNGCG ERKKHDRIYD YDVYNDLGKP ESKRPVLGGK EHPYPRRCRT GRPRSKTDPS 310 320 330 340 350 360 SEEESHKKGE MYVPRDETFT ERKEQAFLTK QLLSQLHGLC TGLKVNKDIL PSFPTLASID 370 380 390 400 410 420 ALYDDDFRNQ PVQPEGGKVR LILDLLAKEL VHLVKLEGAE FVEGIRRVFK FETPEIHDMD 430 440 450 460 470 480 KLAWFRDEEF ARQTLAGMNP LSIQLVTELP IVSKLDELKY GPADSLITKE LIEKQINRIM 490 500 510 520 530 540 TAEEAVAQKK LFMLDYHDLL LPYVHRVRKL DNKTMYGSRT LFFLADDGTL RPIAIELTRP 550 560 570 580 590 600 KSPHKQQWRK VFTPGSGYSG SVTGSWEWQL AKIHVLSHDT GYHQLVSHWL RTHCCVEPYV 610 620 630 640 650 660 IAANRQLSQM HPIYRLLHPH FRFTMEINAQ ARGMLICADG IIEKTFSPGE FSMEISSAAY 670 680 690 700 710 720 DKQWRFDMEA LPEDLIRRGM AVRGEDGKLE LAIEDYPYAN DGLLVWDAIK QWASDYVAHY 730 740 750 760 770 780 YPCAVDIVDD EELQDWWTEV RTKGHPDKQD EPWWPELDCH ESLVQVLATI MWVTSAHHAA 790 800 810 820 830 840 VNFGQYPMAG YVPNHPSIAR RNMPCEMGPE EMLAFKAAPE KVWLDTLPSQ LQTVMVMATL 850 860 870 880 890 900 DLLSSHASDE EYMGTHQEPA WQRDGEVDKA FQVFQKKMRD IAEQVEEWNK DDSRRNRHGA 910 920 930 GVVPYVLLRP LNGNPMDAKT VMEMGIPNSI SI

References

[1]

"Jasmonate-induced lipid peroxidation in barley leaves initiated by distinct 13-LOX forms of the chloroplast." Bachmann A., Hause B., Maucher H., Garbe E., Voeroes K., Weichert H., Wasternack C., Feussner I. Biol. Chem. 383:1645-1657(2002) [PubMed: 12452441] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Strain: cv. Salome. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ507212 mRNA. Translation: CAD45186.1.
3D structure databases
HSSP	HSSP built from PDB template 2SBL based on UniProtKB P08170.
ModBase	Search...
Organism-specific databases
Gramene	Q8GSM3.
Enzyme and pathway databases
BRENDA	1.13.11.12. 283.
Family and domain databases
InterPro	IPR000907. LipOase. IPR013819. LipOase_C. IPR001024. LipOase_LH2. IPR001246. LipOase_pln. [Graphical view]
Gene3D	G3DSA:2.60.60.20. Lipase_LipOase. 1 hit.
PANTHER	PTHR11771. LipOase. 1 hit.
Pfam	PF00305. Lipoxygenase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PRINTS	PR00087. LIPOXYGENASE. PR00468. PLTLPOXGNASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
PROSITE	PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LOX22_HORVU

Accession

Primary (citable) accession number: Q8GSM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)