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Protein

Lipoxygenase 2.2, chloroplastic

Gene

LOX2.2

Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. This enzyme exhibits linoleate 13-lipoxygenase activity.

Catalytic activityi

Linoleate + O2 = (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate.
Alpha-linolenate + O2 = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate.

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit. Iron is tightly bound.PROSITE-ProRule annotation

Pathway: oxylipin biosynthesis

This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi588 – 5881Iron; catalyticPROSITE-ProRule annotation
Metal bindingi593 – 5931Iron; catalyticPROSITE-ProRule annotation
Metal bindingi778 – 7781Iron; catalyticPROSITE-ProRule annotation
Metal bindingi782 – 7821Iron; catalyticPROSITE-ProRule annotation
Metal bindingi932 – 9321Iron; via carboxylate; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00382.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoxygenase 2.2, chloroplastic (EC:1.13.11.12)
Alternative name(s):
LOX2:Hv:2
Gene namesi
Name:LOX2.2
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticeaeHordeum

Organism-specific databases

GrameneiQ8GSM3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 932Lipoxygenase 2.2, chloroplasticPRO_0000018324
Transit peptidei1 – ?ChloroplastSequence Analysis

Interactioni

Protein-protein interaction databases

STRINGi4513.MLOC_69781.2.

Structurei

3D structure databases

ProteinModelPortaliQ8GSM3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini79 – 219141PLATPROSITE-ProRule annotationAdd
BLAST
Domaini223 – 932710LipoxygenasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated
Contains 1 lipoxygenase domain.PROSITE-ProRule annotation
Contains 1 PLAT domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiIPR008976. Lipase_LipOase.
IPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_plant.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 1 hit.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8GSM3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTATKPLVG ARAVPLSRRA SFLVAEARRK PSTNARRTRV GSTSTTTTTT
60 70 80 90 100
TILTDVNGPA LTTVAKPGHQ YDLKQTVEMK ATVSVHMKSF WWSDEKKERA
110 120 130 140 150
RDWAYDLILG SWLTLELVSS ELDPKTGQEH DVISGKLKHS RETEKDYDLY
160 170 180 190 200
EAIFTCRHRL APSGAVRLVN YHHTEMLLGE VKIFPAGEDP TKSSAVTLFH
210 220 230 240 250
CQSWIDPSHC SPDKRTFFPV EKSYIPSQTP KGVEKLRKSE LEALRGNGCG
260 270 280 290 300
ERKKHDRIYD YDVYNDLGKP ESKRPVLGGK EHPYPRRCRT GRPRSKTDPS
310 320 330 340 350
SEEESHKKGE MYVPRDETFT ERKEQAFLTK QLLSQLHGLC TGLKVNKDIL
360 370 380 390 400
PSFPTLASID ALYDDDFRNQ PVQPEGGKVR LILDLLAKEL VHLVKLEGAE
410 420 430 440 450
FVEGIRRVFK FETPEIHDMD KLAWFRDEEF ARQTLAGMNP LSIQLVTELP
460 470 480 490 500
IVSKLDELKY GPADSLITKE LIEKQINRIM TAEEAVAQKK LFMLDYHDLL
510 520 530 540 550
LPYVHRVRKL DNKTMYGSRT LFFLADDGTL RPIAIELTRP KSPHKQQWRK
560 570 580 590 600
VFTPGSGYSG SVTGSWEWQL AKIHVLSHDT GYHQLVSHWL RTHCCVEPYV
610 620 630 640 650
IAANRQLSQM HPIYRLLHPH FRFTMEINAQ ARGMLICADG IIEKTFSPGE
660 670 680 690 700
FSMEISSAAY DKQWRFDMEA LPEDLIRRGM AVRGEDGKLE LAIEDYPYAN
710 720 730 740 750
DGLLVWDAIK QWASDYVAHY YPCAVDIVDD EELQDWWTEV RTKGHPDKQD
760 770 780 790 800
EPWWPELDCH ESLVQVLATI MWVTSAHHAA VNFGQYPMAG YVPNHPSIAR
810 820 830 840 850
RNMPCEMGPE EMLAFKAAPE KVWLDTLPSQ LQTVMVMATL DLLSSHASDE
860 870 880 890 900
EYMGTHQEPA WQRDGEVDKA FQVFQKKMRD IAEQVEEWNK DDSRRNRHGA
910 920 930
GVVPYVLLRP LNGNPMDAKT VMEMGIPNSI SI
Length:932
Mass (Da):106,493
Last modified:March 1, 2003 - v1
Checksum:i64D80DF4370F30E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ507212 mRNA. Translation: CAD45186.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ507212 mRNA. Translation: CAD45186.1.

3D structure databases

ProteinModelPortaliQ8GSM3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4513.MLOC_69781.2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

GrameneiQ8GSM3.

Enzyme and pathway databases

UniPathwayiUPA00382.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiIPR008976. Lipase_LipOase.
IPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_plant.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 1 hit.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Jasmonate-induced lipid peroxidation in barley leaves initiated by distinct 13-LOX forms of the chloroplast."
    Bachmann A., Hause B., Maucher H., Garbe E., Voeroes K., Weichert H., Wasternack C., Feussner I.
    Biol. Chem. 383:1645-1657(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
    Strain: cv. Salome.
    Tissue: Leaf.

Entry informationi

Entry nameiLOX22_HORVU
AccessioniPrimary (citable) accession number: Q8GSM3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: March 1, 2003
Last modified: June 24, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.