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Q8GSM2

- LOX23_HORVU

UniProt

Q8GSM2 - LOX23_HORVU

Protein

Lipoxygenase 2.3, chloroplastic

Gene

LOX2.3

Organism
Hordeum vulgare (Barley)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. This enzyme exhibits linoleate 13-lipoxygenase activity.

    Catalytic activityi

    Linoleate + O2 = (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate.
    Alpha-linolenate + O2 = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate.

    Cofactori

    Binds 1 iron ion per subunit. Iron is tightly bound By similarity.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi556 – 5561Iron; catalyticPROSITE-ProRule annotation
    Metal bindingi561 – 5611Iron; catalyticPROSITE-ProRule annotation
    Metal bindingi746 – 7461Iron; catalyticPROSITE-ProRule annotation
    Metal bindingi750 – 7501Iron; catalyticPROSITE-ProRule annotation
    Metal bindingi896 – 8961Iron; via carboxylate; catalyticPROSITE-ProRule annotation

    GO - Molecular functioni

    1. iron ion binding Source: InterPro
    2. linoleate 13S-lipoxygenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. lipid oxidation Source: EnsemblPlants/Gramene
    2. oxylipin biosynthetic process Source: UniProtKB-UniPathway
    3. response to bacterium Source: EnsemblPlants/Gramene
    4. response to fungus Source: EnsemblPlants/Gramene
    5. response to herbivore Source: EnsemblPlants/Gramene
    6. response to jasmonic acid Source: EnsemblPlants/Gramene
    7. response to wounding Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Dioxygenase, Oxidoreductase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00382.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoxygenase 2.3, chloroplastic (EC:1.13.11.12)
    Alternative name(s):
    LOX2:Hv:3
    Gene namesi
    Name:LOX2.3
    OrganismiHordeum vulgare (Barley)
    Taxonomic identifieri4513 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

    Organism-specific databases

    GrameneiQ8GSM2.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast envelope Source: EnsemblPlants/Gramene
    2. chloroplast stroma Source: EnsemblPlants/Gramene
    3. chloroplast thylakoid membrane Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 896Lipoxygenase 2.3, chloroplasticPRO_0000018325
    Transit peptidei1 – ?ChloroplastSequence Analysis

    Proteomic databases

    PRIDEiQ8GSM2.

    Expressioni

    Gene expression databases

    GenevestigatoriQ8GSM2.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8GSM2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini80 – 199120PLATPROSITE-ProRule annotationAdd
    BLAST
    Domaini202 – 896695LipoxygenasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the lipoxygenase family.Curated
    Contains 1 lipoxygenase domain.PROSITE-ProRule annotation
    Contains 1 PLAT domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di2.60.60.20. 1 hit.
    4.10.372.10. 1 hit.
    InterProiIPR008976. Lipase_LipOase.
    IPR000907. LipOase.
    IPR013819. LipOase_C.
    IPR020834. LipOase_CS.
    IPR020833. LipOase_Fe_BS.
    IPR001246. LipOase_pln.
    IPR027433. Lipoxygenase_domain_3.
    IPR001024. PLAT/LH2_dom.
    [Graphical view]
    PANTHERiPTHR11771. PTHR11771. 1 hit.
    PfamiPF00305. Lipoxygenase. 1 hit.
    PF01477. PLAT. 1 hit.
    [Graphical view]
    PRINTSiPR00087. LIPOXYGENASE.
    PR00468. PLTLPOXGNASE.
    SMARTiSM00308. LH2. 1 hit.
    [Graphical view]
    SUPFAMiSSF48484. SSF48484. 1 hit.
    SSF49723. SSF49723. 1 hit.
    PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
    PS00081. LIPOXYGENASE_2. 1 hit.
    PS51393. LIPOXYGENASE_3. 1 hit.
    PS50095. PLAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8GSM2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIHLKQPLVL SAQSSNVASP LFVAGGQQRR ASGAGRTCSG RRLSARRISC    50
    ASTEEAVGVS TSVTTKERAL TVTAIVTAQV PTSVYVARGL DDIQDLFGKT 100
    LLLELVSSEL DPKTGRERER VKGFAHMTLK EGTYEAKMSV PASFGPVGAV 150
    LVENEHHREM FIKDIKLITG GDESTAITFD VASWVHSKFD DPEPRAFFTV 200
    KSYLPSQTPP GIEALRKKEL ETLRGDGHSE RKFHERVYDY DTYNDLGDPD 250
    KNIDHKRPVL GTKEHPYPRR CRTGRPKTLY DPETETRSSP VYVPRDEQFS 300
    DVKGRTFSAT TLRSGLHAIL PAVAPLLNNS HGFSHFPAID ALYSDGIPLP 350
    VDGHGGNSFN VINDVIPRVV QMIEDTTEHV LRFEVPEMLE RDRFSWFRDE 400
    EFARQTLAGL NPICIRRLTE FPIVSKLDPA VYGPAESALS KEILEKMMNG 450
    RMTVEEAMEK KRLFLLDYHD VFLPYVHRVR ELPDTTLYGS RTVFFLSDEG 500
    TLMPLAIELT RPQSPTKPQW KRAFTHGSDA TESWLWKLAK AHVLTHDTGY 550
    HQLVSHWLRT HACVEPYIIA TNRQLSRMHP VYRLLHPHFR YTMEINALAR 600
    EALINADGII EEAFLAGKYS IELSSVAYGA AWQFNTEALP EDLINRGLAV 650
    RRDDGELELA IKDYPYADDG LLIWGSIKQW ASDYVDFYYK SDGDVAGDEE 700
    LRAWWEEVRT KGHADKKDEP WWPVCDTKEN LVQILTIIMW VTSGHHAAVN 750
    FGQYHYAGYF PNRPTVVRRN IPVEENRDDE MKKFMARPEE VLLQSLPSQM 800
    QAIKVMATLD ILSSHSPDEE YMGEYAEPAW LAEPMVKAAF EKFSGRLKEA 850
    EGTIDMRNNN PENKNRCGAG IVPYELLKPF SEPGVTGRGI PNSISI 896
    Length:896
    Mass (Da):101,234
    Last modified:March 1, 2003 - v1
    Checksum:i31511189946B866A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ507213 mRNA. Translation: CAD45187.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ507213 mRNA. Translation: CAD45187.1 .

    3D structure databases

    ProteinModelPortali Q8GSM2.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q8GSM2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    Gramenei Q8GSM2.

    Enzyme and pathway databases

    UniPathwayi UPA00382 .

    Gene expression databases

    Genevestigatori Q8GSM2.

    Family and domain databases

    Gene3Di 2.60.60.20. 1 hit.
    4.10.372.10. 1 hit.
    InterProi IPR008976. Lipase_LipOase.
    IPR000907. LipOase.
    IPR013819. LipOase_C.
    IPR020834. LipOase_CS.
    IPR020833. LipOase_Fe_BS.
    IPR001246. LipOase_pln.
    IPR027433. Lipoxygenase_domain_3.
    IPR001024. PLAT/LH2_dom.
    [Graphical view ]
    PANTHERi PTHR11771. PTHR11771. 1 hit.
    Pfami PF00305. Lipoxygenase. 1 hit.
    PF01477. PLAT. 1 hit.
    [Graphical view ]
    PRINTSi PR00087. LIPOXYGENASE.
    PR00468. PLTLPOXGNASE.
    SMARTi SM00308. LH2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48484. SSF48484. 1 hit.
    SSF49723. SSF49723. 1 hit.
    PROSITEi PS00711. LIPOXYGENASE_1. 1 hit.
    PS00081. LIPOXYGENASE_2. 1 hit.
    PS51393. LIPOXYGENASE_3. 1 hit.
    PS50095. PLAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Jasmonate-induced lipid peroxidation in barley leaves initiated by distinct 13-LOX forms of the chloroplast."
      Bachmann A., Hause B., Maucher H., Garbe E., Voeroes K., Weichert H., Wasternack C., Feussner I.
      Biol. Chem. 383:1645-1657(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
      Strain: cv. Salome.
      Tissue: Leaf.

    Entry informationi

    Entry nameiLOX23_HORVU
    AccessioniPrimary (citable) accession number: Q8GSM2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2003
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3