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Protein

Lipoxygenase 2.3, chloroplastic

Gene

LOX2.3

Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. This enzyme exhibits linoleate 13-lipoxygenase activity.

Catalytic activityi

Linoleate + O2 = (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate.
Alpha-linolenate + O2 = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate.

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit. Iron is tightly bound.PROSITE-ProRule annotation

Pathwayi: oxylipin biosynthesis

This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi556Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi561Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi746Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi750Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi896Iron; via carboxylate; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • green leaf volatile biosynthetic process Source: AgBase
  • oxylipin biosynthetic process Source: UniProtKB-UniPathway
  • response to jasmonic acid Source: AgBase
  • response to salicylic acid Source: AgBase

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis
LigandIron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00382

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoxygenase 2.3, chloroplastic (EC:1.13.11.12)
Alternative name(s):
LOX2:Hv:3
Gene namesi
Name:LOX2.3
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000018325? – 896Lipoxygenase 2.3, chloroplastic
Transit peptidei1 – ?ChloroplastSequence analysis

Proteomic databases

PRIDEiQ8GSM2

Expressioni

Gene expression databases

ExpressionAtlasiQ8GSM2 differential

Structurei

3D structure databases

ProteinModelPortaliQ8GSM2
SMRiQ8GSM2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini80 – 199PLATPROSITE-ProRule annotationAdd BLAST120
Domaini202 – 896LipoxygenasePROSITE-ProRule annotationAdd BLAST695

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

KOiK00454

Family and domain databases

Gene3Di4.10.372.10, 1 hit
InterProiView protein in InterPro
IPR000907 LipOase
IPR013819 LipOase_C
IPR036226 LipOase_C_sf
IPR020834 LipOase_CS
IPR020833 LipOase_Fe_BS
IPR001246 LipOase_plant
IPR027433 Lipoxygenase_dom_3
IPR001024 PLAT/LH2_dom
IPR036392 PLAT/LH2_dom_sf
PANTHERiPTHR11771 PTHR11771, 1 hit
PfamiView protein in Pfam
PF00305 Lipoxygenase, 1 hit
PF01477 PLAT, 1 hit
PRINTSiPR00087 LIPOXYGENASE
PR00468 PLTLPOXGNASE
SMARTiView protein in SMART
SM00308 LH2, 1 hit
SUPFAMiSSF48484 SSF48484, 1 hit
SSF49723 SSF49723, 1 hit
PROSITEiView protein in PROSITE
PS00711 LIPOXYGENASE_1, 1 hit
PS00081 LIPOXYGENASE_2, 1 hit
PS51393 LIPOXYGENASE_3, 1 hit
PS50095 PLAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8GSM2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIHLKQPLVL SAQSSNVASP LFVAGGQQRR ASGAGRTCSG RRLSARRISC
60 70 80 90 100
ASTEEAVGVS TSVTTKERAL TVTAIVTAQV PTSVYVARGL DDIQDLFGKT
110 120 130 140 150
LLLELVSSEL DPKTGRERER VKGFAHMTLK EGTYEAKMSV PASFGPVGAV
160 170 180 190 200
LVENEHHREM FIKDIKLITG GDESTAITFD VASWVHSKFD DPEPRAFFTV
210 220 230 240 250
KSYLPSQTPP GIEALRKKEL ETLRGDGHSE RKFHERVYDY DTYNDLGDPD
260 270 280 290 300
KNIDHKRPVL GTKEHPYPRR CRTGRPKTLY DPETETRSSP VYVPRDEQFS
310 320 330 340 350
DVKGRTFSAT TLRSGLHAIL PAVAPLLNNS HGFSHFPAID ALYSDGIPLP
360 370 380 390 400
VDGHGGNSFN VINDVIPRVV QMIEDTTEHV LRFEVPEMLE RDRFSWFRDE
410 420 430 440 450
EFARQTLAGL NPICIRRLTE FPIVSKLDPA VYGPAESALS KEILEKMMNG
460 470 480 490 500
RMTVEEAMEK KRLFLLDYHD VFLPYVHRVR ELPDTTLYGS RTVFFLSDEG
510 520 530 540 550
TLMPLAIELT RPQSPTKPQW KRAFTHGSDA TESWLWKLAK AHVLTHDTGY
560 570 580 590 600
HQLVSHWLRT HACVEPYIIA TNRQLSRMHP VYRLLHPHFR YTMEINALAR
610 620 630 640 650
EALINADGII EEAFLAGKYS IELSSVAYGA AWQFNTEALP EDLINRGLAV
660 670 680 690 700
RRDDGELELA IKDYPYADDG LLIWGSIKQW ASDYVDFYYK SDGDVAGDEE
710 720 730 740 750
LRAWWEEVRT KGHADKKDEP WWPVCDTKEN LVQILTIIMW VTSGHHAAVN
760 770 780 790 800
FGQYHYAGYF PNRPTVVRRN IPVEENRDDE MKKFMARPEE VLLQSLPSQM
810 820 830 840 850
QAIKVMATLD ILSSHSPDEE YMGEYAEPAW LAEPMVKAAF EKFSGRLKEA
860 870 880 890
EGTIDMRNNN PENKNRCGAG IVPYELLKPF SEPGVTGRGI PNSISI
Length:896
Mass (Da):101,234
Last modified:March 1, 2003 - v1
Checksum:i31511189946B866A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ507213 mRNA Translation: CAD45187.1

Genome annotation databases

KEGGiag:CAD45187

Similar proteinsi

Entry informationi

Entry nameiLOX23_HORVU
AccessioniPrimary (citable) accession number: Q8GSM2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: March 1, 2003
Last modified: March 28, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health