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Q8GSM2 (LOX23_HORVU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lipoxygenase 2.3, chloroplastic

EC=1.13.11.12
Alternative name(s):
LOX2:Hv:3
Gene names
Name:LOX2.3
OrganismHordeum vulgare (Barley)
Taxonomic identifier4513 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Protein attributes

Sequence length896 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. This enzyme exhibits linoleate 13-lipoxygenase activity.

Catalytic activity

Linoleate + O2 = (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate.

Alpha-linolenate + O2 = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate.

Cofactor

Binds 1 iron ion per subunit. Iron is tightly bound By similarity.

Pathway

Lipid metabolism; oxylipin biosynthesis.

Subcellular location

Plastidchloroplast.

Sequence similarities

Belongs to the lipoxygenase family.

Contains 1 lipoxygenase domain.

Contains 1 PLAT domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Chloroplast Potential
Chain? – 896Lipoxygenase 2.3, chloroplasticPRO_0000018325

Regions

Domain80 – 199120PLAT
Domain202 – 896695Lipoxygenase

Sites

Metal binding5561Iron; catalytic By similarity
Metal binding5611Iron; catalytic By similarity
Metal binding7461Iron; catalytic By similarity
Metal binding7501Iron; catalytic By similarity
Metal binding8961Iron; via carboxylate; catalytic By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8GSM2 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 31511189946B866A

FASTA896101,234
        10         20         30         40         50         60 
MIHLKQPLVL SAQSSNVASP LFVAGGQQRR ASGAGRTCSG RRLSARRISC ASTEEAVGVS 

        70         80         90        100        110        120 
TSVTTKERAL TVTAIVTAQV PTSVYVARGL DDIQDLFGKT LLLELVSSEL DPKTGRERER 

       130        140        150        160        170        180 
VKGFAHMTLK EGTYEAKMSV PASFGPVGAV LVENEHHREM FIKDIKLITG GDESTAITFD 

       190        200        210        220        230        240 
VASWVHSKFD DPEPRAFFTV KSYLPSQTPP GIEALRKKEL ETLRGDGHSE RKFHERVYDY 

       250        260        270        280        290        300 
DTYNDLGDPD KNIDHKRPVL GTKEHPYPRR CRTGRPKTLY DPETETRSSP VYVPRDEQFS 

       310        320        330        340        350        360 
DVKGRTFSAT TLRSGLHAIL PAVAPLLNNS HGFSHFPAID ALYSDGIPLP VDGHGGNSFN 

       370        380        390        400        410        420 
VINDVIPRVV QMIEDTTEHV LRFEVPEMLE RDRFSWFRDE EFARQTLAGL NPICIRRLTE 

       430        440        450        460        470        480 
FPIVSKLDPA VYGPAESALS KEILEKMMNG RMTVEEAMEK KRLFLLDYHD VFLPYVHRVR 

       490        500        510        520        530        540 
ELPDTTLYGS RTVFFLSDEG TLMPLAIELT RPQSPTKPQW KRAFTHGSDA TESWLWKLAK 

       550        560        570        580        590        600 
AHVLTHDTGY HQLVSHWLRT HACVEPYIIA TNRQLSRMHP VYRLLHPHFR YTMEINALAR 

       610        620        630        640        650        660 
EALINADGII EEAFLAGKYS IELSSVAYGA AWQFNTEALP EDLINRGLAV RRDDGELELA 

       670        680        690        700        710        720 
IKDYPYADDG LLIWGSIKQW ASDYVDFYYK SDGDVAGDEE LRAWWEEVRT KGHADKKDEP 

       730        740        750        760        770        780 
WWPVCDTKEN LVQILTIIMW VTSGHHAAVN FGQYHYAGYF PNRPTVVRRN IPVEENRDDE 

       790        800        810        820        830        840 
MKKFMARPEE VLLQSLPSQM QAIKVMATLD ILSSHSPDEE YMGEYAEPAW LAEPMVKAAF 

       850        860        870        880        890 
EKFSGRLKEA EGTIDMRNNN PENKNRCGAG IVPYELLKPF SEPGVTGRGI PNSISI 

« Hide

References

[1]"Jasmonate-induced lipid peroxidation in barley leaves initiated by distinct 13-LOX forms of the chloroplast."
Bachmann A., Hause B., Maucher H., Garbe E., Voeroes K., Weichert H., Wasternack C., Feussner I.
Biol. Chem. 383:1645-1657(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
Strain: cv. Salome.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ507213 mRNA. Translation: CAD45187.1.

3D structure databases

ProteinModelPortalQ8GSM2.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ8GSM2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneQ8GSM2.

Enzyme and pathway databases

UniPathwayUPA00382.

Gene expression databases

GenevestigatorQ8GSM2.

Family and domain databases

Gene3D2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProIPR008976. Lipase_LipOase.
IPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_pln.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERPTHR11771. PTHR11771. 1 hit.
PfamPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLOX23_HORVU
AccessionPrimary (citable) accession number: Q8GSM2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: March 1, 2003
Last modified: June 11, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways