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Protein

Lipoxygenase 2.3, chloroplastic

Gene

LOX2.3

Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. This enzyme exhibits linoleate 13-lipoxygenase activity.

Catalytic activityi

Linoleate + O2 = (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate.
Alpha-linolenate + O2 = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate.

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit. Iron is tightly bound.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi556 – 5561Iron; catalyticPROSITE-ProRule annotation
Metal bindingi561 – 5611Iron; catalyticPROSITE-ProRule annotation
Metal bindingi746 – 7461Iron; catalyticPROSITE-ProRule annotation
Metal bindingi750 – 7501Iron; catalyticPROSITE-ProRule annotation
Metal bindingi896 – 8961Iron; via carboxylate; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

  1. linoleate 13S-lipoxygenase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. oxylipin biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00382.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoxygenase 2.3, chloroplastic (EC:1.13.11.12)
Alternative name(s):
LOX2:Hv:3
Gene namesi
Name:LOX2.3
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Organism-specific databases

GrameneiQ8GSM2.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 896Lipoxygenase 2.3, chloroplasticPRO_0000018325
Transit peptidei1 – ?ChloroplastSequence Analysis

Proteomic databases

PRIDEiQ8GSM2.

Expressioni

Gene expression databases

ExpressionAtlasiQ8GSM2. baseline.
GenevestigatoriQ8GSM2.

Structurei

3D structure databases

ProteinModelPortaliQ8GSM2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 199120PLATPROSITE-ProRule annotationAdd
BLAST
Domaini202 – 896695LipoxygenasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated
Contains 1 lipoxygenase domain.PROSITE-ProRule annotation
Contains 1 PLAT domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiIPR008976. Lipase_LipOase.
IPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_plant.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 1 hit.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8GSM2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIHLKQPLVL SAQSSNVASP LFVAGGQQRR ASGAGRTCSG RRLSARRISC
60 70 80 90 100
ASTEEAVGVS TSVTTKERAL TVTAIVTAQV PTSVYVARGL DDIQDLFGKT
110 120 130 140 150
LLLELVSSEL DPKTGRERER VKGFAHMTLK EGTYEAKMSV PASFGPVGAV
160 170 180 190 200
LVENEHHREM FIKDIKLITG GDESTAITFD VASWVHSKFD DPEPRAFFTV
210 220 230 240 250
KSYLPSQTPP GIEALRKKEL ETLRGDGHSE RKFHERVYDY DTYNDLGDPD
260 270 280 290 300
KNIDHKRPVL GTKEHPYPRR CRTGRPKTLY DPETETRSSP VYVPRDEQFS
310 320 330 340 350
DVKGRTFSAT TLRSGLHAIL PAVAPLLNNS HGFSHFPAID ALYSDGIPLP
360 370 380 390 400
VDGHGGNSFN VINDVIPRVV QMIEDTTEHV LRFEVPEMLE RDRFSWFRDE
410 420 430 440 450
EFARQTLAGL NPICIRRLTE FPIVSKLDPA VYGPAESALS KEILEKMMNG
460 470 480 490 500
RMTVEEAMEK KRLFLLDYHD VFLPYVHRVR ELPDTTLYGS RTVFFLSDEG
510 520 530 540 550
TLMPLAIELT RPQSPTKPQW KRAFTHGSDA TESWLWKLAK AHVLTHDTGY
560 570 580 590 600
HQLVSHWLRT HACVEPYIIA TNRQLSRMHP VYRLLHPHFR YTMEINALAR
610 620 630 640 650
EALINADGII EEAFLAGKYS IELSSVAYGA AWQFNTEALP EDLINRGLAV
660 670 680 690 700
RRDDGELELA IKDYPYADDG LLIWGSIKQW ASDYVDFYYK SDGDVAGDEE
710 720 730 740 750
LRAWWEEVRT KGHADKKDEP WWPVCDTKEN LVQILTIIMW VTSGHHAAVN
760 770 780 790 800
FGQYHYAGYF PNRPTVVRRN IPVEENRDDE MKKFMARPEE VLLQSLPSQM
810 820 830 840 850
QAIKVMATLD ILSSHSPDEE YMGEYAEPAW LAEPMVKAAF EKFSGRLKEA
860 870 880 890
EGTIDMRNNN PENKNRCGAG IVPYELLKPF SEPGVTGRGI PNSISI
Length:896
Mass (Da):101,234
Last modified:March 1, 2003 - v1
Checksum:i31511189946B866A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ507213 mRNA. Translation: CAD45187.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ507213 mRNA. Translation: CAD45187.1.

3D structure databases

ProteinModelPortaliQ8GSM2.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ8GSM2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

GrameneiQ8GSM2.

Enzyme and pathway databases

UniPathwayiUPA00382.

Gene expression databases

ExpressionAtlasiQ8GSM2. baseline.
GenevestigatoriQ8GSM2.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiIPR008976. Lipase_LipOase.
IPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_plant.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 1 hit.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Jasmonate-induced lipid peroxidation in barley leaves initiated by distinct 13-LOX forms of the chloroplast."
    Bachmann A., Hause B., Maucher H., Garbe E., Voeroes K., Weichert H., Wasternack C., Feussner I.
    Biol. Chem. 383:1645-1657(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
    Strain: cv. Salome.
    Tissue: Leaf.

Entry informationi

Entry nameiLOX23_HORVU
AccessioniPrimary (citable) accession number: Q8GSM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: March 1, 2003
Last modified: March 4, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.