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Protein

Calmodulin-binding transcription activator 3

Gene

CAMTA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription activator that binds to the DNA consensus sequence 5'-[ACG]CGCG[GTC]-3'. Binds calmodulin in a calcium-dependent manner in vitro (PubMed:12218065). Regulates transcriptional activity in response to calcium signals (Probable). Involved in freezing tolerance in association with CAMTA1 and CAMTA2 (PubMed:23581962). Required for the cold-induced expression of DREB1B/CBF1, DREB1C/CBF2, ZAT12 and GOLS3 (PubMed:19270186). Involved in response to cold. Contributes together with CAMTA5 to the positive regulation of the cold-induced expression of DREB1A/CBF3, DREB1B/CBF1 and DREB1C/CBF2 (PubMed:28351986). Involved together with CAMTA2 and CAMTA4 in the positive regulation of a general stress response (GSR) (PubMed:25039701). Involved in the regulation of GSR amplitude downstream of MEKK1 (PubMed:25157030). Involved in the regulation of a set of genes involved in defense responses against pathogens (PubMed:18298954). Involved in the regulation of both basal resistance and systemic acquired resistance (SAR) (PubMed:21900483). Acts as negative regulator of plant immunity (PubMed:19122675, PubMed:21900483, PubMed:22345509, PubMed:28407487). Binds to the promoter of the defense-related gene EDS1 and represses its expression (PubMed:19122675). Binds to the promoter of the defense-related gene NDR1 and represses its expression (PubMed:22345509). Involved in defense against insects (PubMed:23072934, PubMed:22371088). Required for tolerance to the generalist herbivore Trichoplusia ni, and contributes to the positive regulation of genes associated with glucosinolate metabolism (PubMed:23072934). Required for tolerance to Bradysia impatiens larvae. Mediates herbivore-induced wound response (PubMed:22371088). Required for wound-induced jasmonate accumulation (PubMed:23072934, PubMed:22371088). Involved in the regulation of ethylene-induced senescence by binding to the promoter of the senescence-inducer gene EIN3 and repressing its expression (PubMed:22345509).1 Publication13 Publications

Miscellaneous

The camta3-3D activation tagging mutant plants exhibit compromised systemic acquired resistance (SAR) and enhanced susceptibility to virulent pathogens.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi15 – 141CG-1PROSITE-ProRule annotationAdd BLAST127

GO - Molecular functioni

GO - Biological processi

  • cellular response to cold Source: TAIR
  • defense response to bacterium Source: TAIR
  • defense response to fungus Source: TAIR
  • leaf senescence Source: TAIR
  • positive regulation of defense response to insect Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: GO_Central
  • regulation of transcription, DNA-templated Source: TAIR
  • response to cold Source: UniProtKB

Keywordsi

Molecular functionActivator, Calmodulin-binding, DNA-binding, Repressor
Biological processPlant defense, Stress response, Transcription, Transcription regulation
LigandCalcium

Names & Taxonomyi

Protein namesi
Recommended name:
Calmodulin-binding transcription activator 31 Publication
Short name:
AtCAMTA31 Publication
Alternative name(s):
Ethylene-induced calmodulin-binding protein 11 Publication
Short name:
EICBP11 Publication
Ethylene-induced calmodulin-binding protein a1 Publication
Short name:
EICBP.a1 Publication
Protein SAR-DEFICIENT 31 Publication
Signal-responsive protein 11 Publication
Short name:
AtSR11 Publication
Gene namesi
Name:CAMTA31 Publication
Synonyms:CMTA3Curated, SARD31 Publication, SR11 Publication
Ordered Locus Names:At2g22300Imported
ORF Names:T26C19.4Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G22300.
TAIRilocus:2060405. AT2G22300.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but the double mutants camta1 and camta3 are impaired in freezing tolerance (PubMed:19270186). No visible phenotype under normal growth conditions, but the double mutants camt1 and camt3 exhibit semi-dwarf phenotypes (PubMed:19270186, PubMed:23581962). No visible phenotype under normal growth conditions, but the double mutants camt2 and camt3 exhibit dwarf phenotypes (PubMed:23581962). Reduced growth, chlorosis, spontaneous lesions and constitutive expression of defense-related genes when grown under 22 degrees Celsius (PubMed:18298954, PubMed:19122675, PubMed:28407487). Enhanced sensitivity to insect herbivores (PubMed:23072934, PubMed:22371088).7 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi855A → V: Gain-of-function mutant. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001144881 – 1032Calmodulin-binding transcription activator 3Add BLAST1032

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei272PhosphoserineCombined sources1
Modified residuei964PhosphoserineBy similarity1

Post-translational modificationi

Ubiquinated during pathogen infection. Ubiquitination leads to its subsequent proteasome-dependent degradation, thus allowing the establishment of plant defense response.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8GSA7.
PRIDEiQ8GSA7.

PTM databases

iPTMnetiQ8GSA7.

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, carpels, and siliques, but not in stigmas or other parts of the flower.3 Publications

Inductioni

By heat shock, UVB, salt, wounding, ethylene and methyl jasmonate (PubMed:11162426, PubMed:12218065). Induced by infection with the fungal pathogen Golovinomyces cichoracearum (powdery mildew) and the bacterial pathogen Pseudomonas syringae pv tomato strain DC3000 (PubMed:22345509).3 Publications

Gene expression databases

ExpressionAtlasiQ8GSA7. baseline and differential.
GenevisibleiQ8GSA7. AT.

Interactioni

Subunit structurei

Interacts with SR1IP1 (PubMed:24528504). Interacts with DSC1 (PubMed:28407487).2 Publications

GO - Molecular functioni

  • calmodulin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi2115. 2 interactors.
DIPiDIP-59735N.
IntActiQ8GSA7. 1 interactor.
MINTiMINT-8064185.
STRINGi3702.AT2G22300.1.

Structurei

3D structure databases

ProteinModelPortaliQ8GSA7.
SMRiQ8GSA7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati661 – 690ANK 1Add BLAST30
Repeati694 – 723ANK 2Add BLAST30
Repeati733 – 762ANK 3Add BLAST30
Domaini852 – 881IQ 1PROSITE-ProRule annotationAdd BLAST30
Domaini875 – 904IQ 2PROSITE-ProRule annotationAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni900 – 922Calmodulin-binding1 PublicationAdd BLAST23

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili945 – 987Sequence analysisAdd BLAST43

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi780 – 785Poly-Ser6
Compositional biasi1003 – 1019Asp-richAdd BLAST17

Sequence similaritiesi

Belongs to the CAMTA family.Curated

Keywords - Domaini

ANK repeat, Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IUMH. Eukaryota.
ENOG4112848. LUCA.
HOGENOMiHOG000240102.
InParanoidiQ8GSA7.
KOiK21596.
OMAiYWEEVES.
OrthoDBiEOG0936043Z.
PhylomeDBiQ8GSA7.

Family and domain databases

CDDicd00204. ANK. 1 hit.
Gene3Di1.25.40.20. 1 hit.
2.60.40.10. 1 hit.
InterProiView protein in InterPro
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR036770. Ankyrin_rpt-contain_sf.
IPR005559. CG-1_dom.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF03859. CG-1. 1 hit.
PF00612. IQ. 2 hits.
PF01833. TIG. 1 hit.
SMARTiView protein in SMART
SM01076. CG-1. 1 hit.
SM00015. IQ. 2 hits.
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiView protein in PROSITE
PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS51437. CG_1. 1 hit.
PS50096. IQ. 2 hits.

Sequencei

Sequence statusi: Complete.

Q8GSA7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEARRFSPV HELDVGQILS EARHRWLRPP EICEILQNYQ RFQISTEPPT
60 70 80 90 100
TPSSGSVFMF DRKVLRYFRK DGHNWRKKKD GKTVKEAHER LKAGSVDVLH
110 120 130 140 150
CYYAHGQDNE NFQRRSYWLL QEELSHIVFV HYLEVKGSRV STSFNRMQRT
160 170 180 190 200
EDAARSPQET GDALTSEHDG YASCSFNQND HSNHSQTTDS ASVNGFHSPE
210 220 230 240 250
LEDAESAYNQ HGSSTAYSHQ ELQQPATGGN LTGFDPYYQI SLTPRDSYQK
260 270 280 290 300
ELRTIPVTDS SIMVDKSKTI NSPGVTNGLK NRKSIDSQTW EEILGNCGSG
310 320 330 340 350
VEALPLQPNS EHEVLDQILE SSFTMQDFAS LQESMVKSQN QELNSGLTSD
360 370 380 390 400
RTVWFQGQDM ELNAISNLAS NEKAPYLSTM KQHLLHGALG EEGLKKMDSF
410 420 430 440 450
NRWMSKELGD VGVIADANES FTQSSSRTYW EEVESEDGSN GHNSRRDMDG
460 470 480 490 500
YVMSPSLSKE QLFSINDFSP SWAYVGCEVV VFVTGKFLKT REETEIGEWS
510 520 530 540 550
CMFGQTEVPA DVISNGILQC VAPMHEAGRV PFYVTCSNRL ACSEVREFEY
560 570 580 590 600
KVAESQVFDR EADDESTIDI LEARFVKLLC SKSENTSPVS GNDSDLSQLS
610 620 630 640 650
EKISLLLFEN DDQLDQMLMN EISQENMKNN LLQEFLKESL HSWLLQKIAE
660 670 680 690 700
GGKGPSVLDE GGQGVLHFAA SLGYNWALEP TIIAGVSVDF RDVNGWTALH
710 720 730 740 750
WAAFFGRERI IGSLIALGAA PGTLTDPNPD FPSGSTPSDL AYANGHKGIA
760 770 780 790 800
GYLSEYALRA HVSLLSLNDK NAETVEMAPS PSSSSLTDSL TAVRNATQAA
810 820 830 840 850
ARIHQVFRAQ SFQKKQLKEF GDKKLGMSEE RALSMLAPKT HKSGRAHSDD
860 870 880 890 900
SVQAAAIRIQ NKFRGYKGRK DYLITRQRII KIQAHVRGYQ FRKNYRKIIW
910 920 930 940 950
SVGVLEKVIL RWRRKGAGLR GFKSEALVEK MQDGTEKEED DDFFKQGRKQ
960 970 980 990 1000
TEDRLQKALA RVKSMVQYPE ARDQYRRLLN VVNDIQESKV EKALENSEAT
1010 1020 1030
CFDDDDDLID IEALLEDDDT LMLPMSSSLW TS
Length:1,032
Mass (Da):116,111
Last modified:March 1, 2003 - v1
Checksum:i4C5E9775A3795862
GO

Sequence cautioni

The sequence AAD23613 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti382Q → E in BAE98628 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY510025 mRNA. Translation: AAR98746.1.
AF303397 mRNA. Translation: AAG37879.1.
AF506697 mRNA. Translation: AAN74651.1.
AC007168 Genomic DNA. Translation: AAD23613.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC07289.1.
CP002685 Genomic DNA. Translation: AEC07290.1.
BT002459 mRNA. Translation: AAO00819.1.
AK226486 mRNA. Translation: BAE98628.1.
PIRiA84611.
RefSeqiNP_001118361.1. NM_001124889.2.
NP_850023.1. NM_179692.3.
UniGeneiAt.48505.
At.71036.

Genome annotation databases

EnsemblPlantsiAT2G22300.1; AT2G22300.1; AT2G22300.
AT2G22300.2; AT2G22300.2; AT2G22300.
GeneIDi816762.
GrameneiAT2G22300.1; AT2G22300.1; AT2G22300.
AT2G22300.2; AT2G22300.2; AT2G22300.
KEGGiath:AT2G22300.

Similar proteinsi

Entry informationi

Entry nameiCMTA3_ARATH
AccessioniPrimary (citable) accession number: Q8GSA7
Secondary accession number(s): Q0WW70, Q9FPR9, Q9SID7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: March 1, 2003
Last modified: December 20, 2017
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families