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Q8GRX1

- BGL34_ARATH

UniProt

Q8GRX1 - BGL34_ARATH

Protein

Myrosinase 4

Gene

TGG4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Hydrolyzes sinigrin and, with lower efficiency, p-nitrophenyl beta-D-glucoside.1 Publication

    Catalytic activityi

    A thioglucoside + H2O = a sugar + a thiol.
    Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

    Kineticsi

    1. KM=245 µM for sinigrin (at pH 4.5)1 Publication
    2. KM=28 mM for p-nitrophenyl beta-D-glucoside (at pH 4.5)1 Publication

    Vmax=12.2 µmol/min/mg enzyme with sinigrin as substrate (at pH 4.5)1 Publication

    Vmax=7.3 µmol/min/mg enzyme with p-nitrophenyl beta-D-glucoside as substrate (at pH 4.5)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei64 – 641SubstrateBy similarity
    Binding sitei165 – 1651SubstrateBy similarity
    Binding sitei210 – 2101SubstrateBy similarity
    Binding sitei211 – 2111AscorbateBy similarity
    Binding sitei280 – 2801AscorbateBy similarity
    Binding sitei351 – 3511SubstrateBy similarity
    Active sitei418 – 4181NucleophilePROSITE-ProRule annotation
    Binding sitei467 – 4671SubstrateBy similarity

    GO - Molecular functioni

    1. beta-glucosidase activity Source: TAIR
    2. thioglucosidase activity Source: TAIR

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. glucosinolate metabolic process Source: TAIR
    3. response to salt stress Source: TAIR

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:GQT-1575-MONOMER.
    SABIO-RKQ8GRX1.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myrosinase 4 (EC:3.2.1.147)
    Alternative name(s):
    Beta-glucosidase 34 (EC:3.2.1.21)
    Short name:
    AtBGLU34
    Sinigrinase 4
    Thioglucosidase 4
    Gene namesi
    Name:TGG4
    Synonyms:BGLU34
    Ordered Locus Names:At1g47600
    ORF Names:F16N3.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G47600.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 511488Myrosinase 4PRO_0000389596Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi31 ↔ 450By similarity
    Disulfide bondi39 ↔ 445By similarity
    Glycosylationi46 – 461N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi53 – 531N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi230 ↔ 233By similarity
    Glycosylationi428 – 4281N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi489 – 4891N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ8GRX1.
    PRIDEiQ8GRX1.

    Expressioni

    Tissue specificityi

    Specifically expressed in roots.1 Publication

    Gene expression databases

    GenevestigatoriQ8GRX1.

    Interactioni

    Protein-protein interaction databases

    BioGridi26394. 1 interaction.
    STRINGi3702.AT1G47600.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8GRX1.
    SMRiQ8GRX1. Positions 46-511.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni474 – 4752Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2723.
    HOGENOMiHOG000088630.
    InParanoidiQ8GRX1.
    KOiK01237.
    OMAiNWSWINS.
    PhylomeDBiQ8GRX1.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q8GRX1-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAIPKAHYSL AVLVLLFVVV SSSQKVCNPE CKAKEPFHCD NTHAFNRTGF    50
    PRNFTFGAAT SAYQIEGAAH RALNGWDYFT HRYPEKVPDR SSGDLACDSY 100
    DLYKDDVKLL KRMNVQAYRL SIAWSRVLPK GRLTGGVDEN GITYYNNLIN 150
    ELKANGIEPY VTIFHWDVPQ TLEDEYGGFL STRIVEDYTN YAELLFQRFG 200
    DRVKFWITLN QPFSLATKGY GDGSYPPGRC TGCELGGDSG VEPYTVAHNQ 250
    LLAHAKTVSL YRKRYQKFQG GKIGTTLIGR WFAPLNEFSE LDKAAAKRAF 300
    DFFVGWFLDP LVYGKYPTIM REMVGDRLPE FTPEQSALVK GSLDFLGLNY 350
    YVTQYATDAP PPTQLNAITD ARVTLGFYRN GVPIGVVAPS FVYYPPGFRQ 400
    ILNYIKDNYK NPLTYITENG VADLDLGNVT LATALADNGR IQNHCSHLSC 450
    LKCAMKDGCN VAGYFAWSLM DNYEFGNGYT LRFGMNWVNF TNPADRKEKA 500
    SGKWFSKFLA K 511
    Length:511
    Mass (Da):57,542
    Last modified:March 1, 2003 - v1
    Checksum:iA977148B22E21D0E
    GO

    Sequence cautioni

    The sequence AAD46026.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FJ268795 Genomic DNA. Translation: ACO95139.1.
    AC007519 Genomic DNA. Translation: AAD46026.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE32191.1.
    BT000471 mRNA. Translation: AAN17448.1.
    BT002202 mRNA. Translation: AAN72213.1.
    BT002458 mRNA. Translation: AAO00818.1.
    PIRiG96516.
    RefSeqiNP_175191.2. NM_103653.2. [Q8GRX1-1]
    UniGeneiAt.25235.

    Genome annotation databases

    EnsemblPlantsiAT1G47600.1; AT1G47600.1; AT1G47600. [Q8GRX1-1]
    GeneIDi841169.
    KEGGiath:AT1G47600.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FJ268795 Genomic DNA. Translation: ACO95139.1 .
    AC007519 Genomic DNA. Translation: AAD46026.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE32191.1 .
    BT000471 mRNA. Translation: AAN17448.1 .
    BT002202 mRNA. Translation: AAN72213.1 .
    BT002458 mRNA. Translation: AAO00818.1 .
    PIRi G96516.
    RefSeqi NP_175191.2. NM_103653.2. [Q8GRX1-1 ]
    UniGenei At.25235.

    3D structure databases

    ProteinModelPortali Q8GRX1.
    SMRi Q8GRX1. Positions 46-511.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 26394. 1 interaction.
    STRINGi 3702.AT1G47600.1-P.

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Proteomic databases

    PaxDbi Q8GRX1.
    PRIDEi Q8GRX1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G47600.1 ; AT1G47600.1 ; AT1G47600 . [Q8GRX1-1 ]
    GeneIDi 841169.
    KEGGi ath:AT1G47600.

    Organism-specific databases

    TAIRi AT1G47600.

    Phylogenomic databases

    eggNOGi COG2723.
    HOGENOMi HOG000088630.
    InParanoidi Q8GRX1.
    KOi K01237.
    OMAi NWSWINS.
    PhylomeDBi Q8GRX1.

    Enzyme and pathway databases

    BioCyci ARA:GQT-1575-MONOMER.
    SABIO-RK Q8GRX1.

    Gene expression databases

    Genevestigatori Q8GRX1.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a new subfamily of thioglucoside glucohydrolases."
      Zhang J.
      Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. Cited for: GENE FAMILY, NOMENCLATURE.
    6. "Myrosinases from root and leaves of Arabidopsis thaliana have different catalytic properties."
      Andersson D., Chakrabarty R., Bejai S., Zhang J., Rask L., Meijer J.
      Phytochemistry 70:1345-1354(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiBGL34_ARATH
    AccessioniPrimary (citable) accession number: Q8GRX1
    Secondary accession number(s): Q9SX92
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    It seems that the absence of a catalytic proton donor in plant myrosinases is not impairing the hydrolysis of glucosinolates.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3