Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Myrosinase 4

Gene

TGG4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes sinigrin and, with lower efficiency, p-nitrophenyl beta-D-glucoside.1 Publication

Catalytic activityi

A thioglucoside + H2O = a sugar + a thiol.
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Kineticsi

  1. KM=245 µM for sinigrin (at pH 4.5)1 Publication
  2. KM=28 mM for p-nitrophenyl beta-D-glucoside (at pH 4.5)1 Publication
  1. Vmax=12.2 µmol/min/mg enzyme with sinigrin as substrate (at pH 4.5)1 Publication
  2. Vmax=7.3 µmol/min/mg enzyme with p-nitrophenyl beta-D-glucoside as substrate (at pH 4.5)1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei64SubstrateBy similarity1
Binding sitei165SubstrateBy similarity1
Binding sitei210SubstrateBy similarity1
Binding sitei211AscorbateBy similarity1
Binding sitei280AscorbateBy similarity1
Binding sitei351SubstrateBy similarity1
Active sitei418NucleophilePROSITE-ProRule annotation1
Binding sitei467SubstrateBy similarity1

GO - Molecular functioni

  • beta-glucosidase activity Source: TAIR
  • scopolin beta-glucosidase activity Source: UniProtKB-EC
  • thioglucosidase activity Source: TAIR

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • glucosinolate catabolic process Source: GO_Central
  • glucosinolate metabolic process Source: TAIR
  • response to hormone Source: GO_Central
  • response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

SABIO-RKQ8GRX1.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Myrosinase 4 (EC:3.2.1.147)
Alternative name(s):
Beta-glucosidase 34 (EC:3.2.1.21)
Short name:
AtBGLU34
Sinigrinase 4
Thioglucosidase 4
Gene namesi
Name:TGG4
Synonyms:BGLU34
Ordered Locus Names:At1g47600
ORF Names:F16N3.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G47600.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000038959624 – 511Myrosinase 4Add BLAST488

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 450By similarity
Disulfide bondi39 ↔ 445By similarity
Glycosylationi46N-linked (GlcNAc...)Sequence analysis1
Glycosylationi53N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi230 ↔ 233By similarity
Glycosylationi428N-linked (GlcNAc...)Sequence analysis1
Glycosylationi489N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8GRX1.

Expressioni

Tissue specificityi

Specifically expressed in roots.1 Publication

Gene expression databases

ExpressionAtlasiQ8GRX1. baseline and differential.
GenevisibleiQ8GRX1. AT.

Interactioni

Protein-protein interaction databases

BioGridi26394. 1 interactor.
STRINGi3702.AT1G47600.1.

Structurei

3D structure databases

ProteinModelPortaliQ8GRX1.
SMRiQ8GRX1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni474 – 475Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0626. Eukaryota.
COG2723. LUCA.
HOGENOMiHOG000088630.
InParanoidiQ8GRX1.
KOiK01237.
OMAiWANTAIS.
OrthoDBiEOG0936077Q.
PhylomeDBiQ8GRX1.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q8GRX1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAIPKAHYSL AVLVLLFVVV SSSQKVCNPE CKAKEPFHCD NTHAFNRTGF
60 70 80 90 100
PRNFTFGAAT SAYQIEGAAH RALNGWDYFT HRYPEKVPDR SSGDLACDSY
110 120 130 140 150
DLYKDDVKLL KRMNVQAYRL SIAWSRVLPK GRLTGGVDEN GITYYNNLIN
160 170 180 190 200
ELKANGIEPY VTIFHWDVPQ TLEDEYGGFL STRIVEDYTN YAELLFQRFG
210 220 230 240 250
DRVKFWITLN QPFSLATKGY GDGSYPPGRC TGCELGGDSG VEPYTVAHNQ
260 270 280 290 300
LLAHAKTVSL YRKRYQKFQG GKIGTTLIGR WFAPLNEFSE LDKAAAKRAF
310 320 330 340 350
DFFVGWFLDP LVYGKYPTIM REMVGDRLPE FTPEQSALVK GSLDFLGLNY
360 370 380 390 400
YVTQYATDAP PPTQLNAITD ARVTLGFYRN GVPIGVVAPS FVYYPPGFRQ
410 420 430 440 450
ILNYIKDNYK NPLTYITENG VADLDLGNVT LATALADNGR IQNHCSHLSC
460 470 480 490 500
LKCAMKDGCN VAGYFAWSLM DNYEFGNGYT LRFGMNWVNF TNPADRKEKA
510
SGKWFSKFLA K
Length:511
Mass (Da):57,542
Last modified:March 1, 2003 - v1
Checksum:iA977148B22E21D0E
GO

Sequence cautioni

The sequence AAD46026 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ268795 Genomic DNA. Translation: ACO95139.1.
AC007519 Genomic DNA. Translation: AAD46026.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32191.1.
BT000471 mRNA. Translation: AAN17448.1.
BT002202 mRNA. Translation: AAN72213.1.
BT002458 mRNA. Translation: AAO00818.1.
PIRiG96516.
RefSeqiNP_175191.2. NM_103653.3. [Q8GRX1-1]
UniGeneiAt.25235.

Genome annotation databases

EnsemblPlantsiAT1G47600.1; AT1G47600.1; AT1G47600. [Q8GRX1-1]
GeneIDi841169.
GrameneiAT1G47600.1; AT1G47600.1; AT1G47600.
KEGGiath:AT1G47600.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ268795 Genomic DNA. Translation: ACO95139.1.
AC007519 Genomic DNA. Translation: AAD46026.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32191.1.
BT000471 mRNA. Translation: AAN17448.1.
BT002202 mRNA. Translation: AAN72213.1.
BT002458 mRNA. Translation: AAO00818.1.
PIRiG96516.
RefSeqiNP_175191.2. NM_103653.3. [Q8GRX1-1]
UniGeneiAt.25235.

3D structure databases

ProteinModelPortaliQ8GRX1.
SMRiQ8GRX1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi26394. 1 interactor.
STRINGi3702.AT1G47600.1.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbiQ8GRX1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G47600.1; AT1G47600.1; AT1G47600. [Q8GRX1-1]
GeneIDi841169.
GrameneiAT1G47600.1; AT1G47600.1; AT1G47600.
KEGGiath:AT1G47600.

Organism-specific databases

TAIRiAT1G47600.

Phylogenomic databases

eggNOGiKOG0626. Eukaryota.
COG2723. LUCA.
HOGENOMiHOG000088630.
InParanoidiQ8GRX1.
KOiK01237.
OMAiWANTAIS.
OrthoDBiEOG0936077Q.
PhylomeDBiQ8GRX1.

Enzyme and pathway databases

SABIO-RKQ8GRX1.

Miscellaneous databases

PROiQ8GRX1.

Gene expression databases

ExpressionAtlasiQ8GRX1. baseline and differential.
GenevisibleiQ8GRX1. AT.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGL34_ARATH
AccessioniPrimary (citable) accession number: Q8GRX1
Secondary accession number(s): Q9SX92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

It seems that the absence of a catalytic proton donor in plant myrosinases is not impairing the hydrolysis of glucosinolates.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.