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Protein

Tubulin-folding cofactor E

Gene

TFCE

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Essential tubulin-folding protein involved in the tubulin folding pathway. Not essential for cell viability. Probably involved in the binding of alpha-tubulin in the multimeric supercomplex.2 Publications

Miscellaneous

Belongs to the PILZ group of genes that disrupt, when mutated, the microtubule cytoskeleton and produce mushroom-shaped ('pilz' in German) embryos.

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR

Keywordsi

Molecular functionChaperone

Names & Taxonomyi

Protein namesi
Recommended name:
Tubulin-folding cofactor E
Short name:
AtTFCE
Alternative name(s):
Protein PFIFFERLING
Gene namesi
Name:TFCE
Synonyms:PFI, TBCE
Ordered Locus Names:At1g71440
ORF Names:F26A9.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G71440
TAIRilocus:2825339 AT1G71440

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Embryo lethality. Embryo development limited to the formation of a few giant cells lacking microtubules but not actin filaments. Failure to localize KNOLLE in mitotic cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004235011 – 531Tubulin-folding cofactor EAdd BLAST531

Proteomic databases

PaxDbiQ8GRL7
PRIDEiQ8GRL7

Expressioni

Gene expression databases

ExpressionAtlasiQ8GRL7 baseline and differential
GenevisibleiQ8GRL7 AT

Interactioni

Subunit structurei

Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state (By similarity).By similarity

Protein-protein interaction databases

STRINGi3702.AT1G71440.1

Structurei

3D structure databases

ProteinModelPortaliQ8GRL7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 76CAP-GlyPROSITE-ProRule annotationAdd BLAST45
Repeati84 – 109LRR 1Add BLAST26
Repeati159 – 183LRR 2Add BLAST25
Repeati185 – 213LRR 3Add BLAST29
Repeati233 – 256LRR 4Add BLAST24
Repeati260 – 284LRR 5Add BLAST25
Repeati285 – 308LRR 6Add BLAST24
Repeati318 – 342LRR 7Add BLAST25
Repeati344 – 366LRR 8Add BLAST23
Repeati474 – 497LRR 9Add BLAST24

Sequence similaritiesi

Belongs to the TBCE family.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiKOG2982 Eukaryota
ENOG41103AE LUCA
HOGENOMiHOG000241155
InParanoidiQ8GRL7
KOiK21768
OMAiRMITVQT
OrthoDBiEOG09360CWE
PhylomeDBiQ8GRL7

Family and domain databases

Gene3Di2.30.30.190, 1 hit
3.80.10.10, 2 hits
InterProiView protein in InterPro
IPR036859 CAP-Gly_dom_sf
IPR000938 CAP-Gly_domain
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
IPR029071 Ubiquitin-like_domsf
PfamiView protein in Pfam
PF01302 CAP_GLY, 1 hit
SMARTiView protein in SMART
SM01052 CAP_GLY, 1 hit
SM00369 LRR_TYP, 6 hits
SUPFAMiSSF54236 SSF54236, 1 hit
SSF74924 SSF74924, 1 hit
PROSITEiView protein in PROSITE
PS00845 CAP_GLY_1, 1 hit
PS50245 CAP_GLY_2, 1 hit
PS51450 LRR, 6 hits

Sequencei

Sequence statusi: Complete.

Q8GRL7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAESSNESF IIGQRVHSLN DSRRVGTVKY VGDVEGYSGT WIGVDWDQDG
60 70 80 90 100
DGKHNGSVNG VFYFNGRSQS SASFVRSQNL SRGITLLQAL ELRYRTISTK
110 120 130 140 150
DEEDEMYVLS AGNRRVSIQL LGGDKIQDKL SRFEELTSAS LSYLGVSSLG
160 170 180 190 200
VSSDLGSILP NLKLLDLTGN LISDWEEIGA LCEQLPALTT LNLSCNSLSS
210 220 230 240 250
DIKSLPQLKN IRVLVLNNSG LSWTQVEILR RSLPGIEELH LMGNMISTIT
260 270 280 290 300
STSSSDDQAF NSLRLLNLDD NCISDWSEVL KLSQLPCLEQ LYLNKNKLSR
310 320 330 340 350
IFQSVNGTES SEKGSDPFPS LSCLLLGANN IGDLASVDAL NGFPQLVDIR
360 370 380 390 400
LSENPISDPV RGGVPRFVLV ARLTKVQVLN GSEVRAREKK DSEIRYVRMV
410 420 430 440 450
MSKLNDKSGE IELLHPRFYE LKKLHGIEDE RASAENSGPK NIASGLISIT
460 470 480 490 500
LKCVGPSMGE KPHLTKKLPG SITVGKLKIL SENFFKLKSI KPRLFLQEEG
510 520 530
SPFPTALDDE TATLLDVGIC DGSTLLVDEE S
Length:531
Mass (Da):58,242
Last modified:March 1, 2003 - v1
Checksum:iDFED24FF5946B9CF
GO

Sequence cautioni

The sequence AAG51824 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti207Q → E in AAM22962 (PubMed:11959844).Curated1
Sequence conflicti207Q → E in AAM63142 (Ref. 6) Curated1
Sequence conflicti347V → L in AAM22962 (PubMed:11959844).Curated1
Sequence conflicti347V → L in AAM63142 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF486853 mRNA Translation: AAM22962.1
AC016163 Genomic DNA Translation: AAG51824.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE35201.1
AK118835 mRNA Translation: BAC43424.1
BT002403 mRNA Translation: AAO00763.1
BT008888 mRNA Translation: AAP68327.1
AY085930 mRNA Translation: AAM63142.1
RefSeqiNP_565017.1, NM_105813.4
UniGeneiAt.35185
At.48359

Genome annotation databases

EnsemblPlantsiAT1G71440.1; AT1G71440.1; AT1G71440
GeneIDi843485
GrameneiAT1G71440.1; AT1G71440.1; AT1G71440
KEGGiath:AT1G71440

Similar proteinsi

Entry informationi

Entry nameiTBCE_ARATH
AccessioniPrimary (citable) accession number: Q8GRL7
Secondary accession number(s): Q8L405, Q9C9I1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 18, 2013
Last sequence update: March 1, 2003
Last modified: April 25, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health