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Protein

Tubulin-folding cofactor E

Gene

TFCE

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Essential tubulin-folding protein involved in the tubulin folding pathway. Not essential for cell viability. Probably involved in the binding of alpha-tubulin in the multimeric supercomplex.2 Publications

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Names & Taxonomyi

Protein namesi
Recommended name:
Tubulin-folding cofactor E
Short name:
AtTFCE
Alternative name(s):
Protein PFIFFERLING
Gene namesi
Name:TFCE
Synonyms:PFI, TBCE
Ordered Locus Names:At1g71440
ORF Names:F26A9.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G71440.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Embryo lethality. Embryo development limited to the formation of a few giant cells lacking microtubules but not actin filaments. Failure to localize KNOLLE in mitotic cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004235011 – 531Tubulin-folding cofactor EAdd BLAST531

Proteomic databases

PaxDbiQ8GRL7.
PRIDEiQ8GRL7.

Expressioni

Gene expression databases

GenevisibleiQ8GRL7. AT.

Interactioni

Subunit structurei

Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state (By similarity).By similarity

Protein-protein interaction databases

STRINGi3702.AT1G71440.1.

Structurei

3D structure databases

ProteinModelPortaliQ8GRL7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 76CAP-GlyPROSITE-ProRule annotationAdd BLAST45
Repeati84 – 109LRR 1Add BLAST26
Repeati159 – 183LRR 2Add BLAST25
Repeati185 – 213LRR 3Add BLAST29
Repeati233 – 256LRR 4Add BLAST24
Repeati260 – 284LRR 5Add BLAST25
Repeati285 – 308LRR 6Add BLAST24
Repeati318 – 342LRR 7Add BLAST25
Repeati344 – 366LRR 8Add BLAST23
Repeati474 – 497LRR 9Add BLAST24

Sequence similaritiesi

Belongs to the TBCE family.Curated
Contains 1 CAP-Gly domain.PROSITE-ProRule annotation
Contains 9 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiKOG2982. Eukaryota.
ENOG41103AE. LUCA.
HOGENOMiHOG000241155.
InParanoidiQ8GRL7.
OMAiVSLRNCA.
OrthoDBiEOG09360CWE.
PhylomeDBiQ8GRL7.

Family and domain databases

Gene3Di2.30.30.190. 1 hit.
3.80.10.10. 3 hits.
InterProiIPR000938. CAP-Gly_domain.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF01302. CAP_GLY. 1 hit.
[Graphical view]
SMARTiSM01052. CAP_GLY. 1 hit.
SM00369. LRR_TYP. 6 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF74924. SSF74924. 1 hit.
PROSITEiPS00845. CAP_GLY_1. 1 hit.
PS50245. CAP_GLY_2. 1 hit.
PS51450. LRR. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8GRL7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAESSNESF IIGQRVHSLN DSRRVGTVKY VGDVEGYSGT WIGVDWDQDG
60 70 80 90 100
DGKHNGSVNG VFYFNGRSQS SASFVRSQNL SRGITLLQAL ELRYRTISTK
110 120 130 140 150
DEEDEMYVLS AGNRRVSIQL LGGDKIQDKL SRFEELTSAS LSYLGVSSLG
160 170 180 190 200
VSSDLGSILP NLKLLDLTGN LISDWEEIGA LCEQLPALTT LNLSCNSLSS
210 220 230 240 250
DIKSLPQLKN IRVLVLNNSG LSWTQVEILR RSLPGIEELH LMGNMISTIT
260 270 280 290 300
STSSSDDQAF NSLRLLNLDD NCISDWSEVL KLSQLPCLEQ LYLNKNKLSR
310 320 330 340 350
IFQSVNGTES SEKGSDPFPS LSCLLLGANN IGDLASVDAL NGFPQLVDIR
360 370 380 390 400
LSENPISDPV RGGVPRFVLV ARLTKVQVLN GSEVRAREKK DSEIRYVRMV
410 420 430 440 450
MSKLNDKSGE IELLHPRFYE LKKLHGIEDE RASAENSGPK NIASGLISIT
460 470 480 490 500
LKCVGPSMGE KPHLTKKLPG SITVGKLKIL SENFFKLKSI KPRLFLQEEG
510 520 530
SPFPTALDDE TATLLDVGIC DGSTLLVDEE S
Length:531
Mass (Da):58,242
Last modified:March 1, 2003 - v1
Checksum:iDFED24FF5946B9CF
GO

Sequence cautioni

The sequence AAG51824 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti207Q → E in AAM22962 (PubMed:11959844).Curated1
Sequence conflicti207Q → E in AAM63142 (Ref. 6) Curated1
Sequence conflicti347V → L in AAM22962 (PubMed:11959844).Curated1
Sequence conflicti347V → L in AAM63142 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF486853 mRNA. Translation: AAM22962.1.
AC016163 Genomic DNA. Translation: AAG51824.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE35201.1.
AK118835 mRNA. Translation: BAC43424.1.
BT002403 mRNA. Translation: AAO00763.1.
BT008888 mRNA. Translation: AAP68327.1.
AY085930 mRNA. Translation: AAM63142.1.
RefSeqiNP_565017.1. NM_105813.4.
UniGeneiAt.35185.
At.48359.

Genome annotation databases

EnsemblPlantsiAT1G71440.1; AT1G71440.1; AT1G71440.
GeneIDi843485.
GrameneiAT1G71440.1; AT1G71440.1; AT1G71440.
KEGGiath:AT1G71440.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF486853 mRNA. Translation: AAM22962.1.
AC016163 Genomic DNA. Translation: AAG51824.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE35201.1.
AK118835 mRNA. Translation: BAC43424.1.
BT002403 mRNA. Translation: AAO00763.1.
BT008888 mRNA. Translation: AAP68327.1.
AY085930 mRNA. Translation: AAM63142.1.
RefSeqiNP_565017.1. NM_105813.4.
UniGeneiAt.35185.
At.48359.

3D structure databases

ProteinModelPortaliQ8GRL7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G71440.1.

Proteomic databases

PaxDbiQ8GRL7.
PRIDEiQ8GRL7.

Protocols and materials databases

DNASUi843485.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G71440.1; AT1G71440.1; AT1G71440.
GeneIDi843485.
GrameneiAT1G71440.1; AT1G71440.1; AT1G71440.
KEGGiath:AT1G71440.

Organism-specific databases

TAIRiAT1G71440.

Phylogenomic databases

eggNOGiKOG2982. Eukaryota.
ENOG41103AE. LUCA.
HOGENOMiHOG000241155.
InParanoidiQ8GRL7.
OMAiVSLRNCA.
OrthoDBiEOG09360CWE.
PhylomeDBiQ8GRL7.

Miscellaneous databases

PROiQ8GRL7.

Gene expression databases

GenevisibleiQ8GRL7. AT.

Family and domain databases

Gene3Di2.30.30.190. 1 hit.
3.80.10.10. 3 hits.
InterProiIPR000938. CAP-Gly_domain.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF01302. CAP_GLY. 1 hit.
[Graphical view]
SMARTiSM01052. CAP_GLY. 1 hit.
SM00369. LRR_TYP. 6 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF74924. SSF74924. 1 hit.
PROSITEiPS00845. CAP_GLY_1. 1 hit.
PS50245. CAP_GLY_2. 1 hit.
PS51450. LRR. 6 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTBCE_ARATH
AccessioniPrimary (citable) accession number: Q8GRL7
Secondary accession number(s): Q8L405, Q9C9I1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 18, 2013
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Belongs to the PILZ group of genes that disrupt, when mutated, the microtubule cytoskeleton and produce mushroom-shaped ('pilz' in German) embryos.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.