Quinohemoprotein alcohol dehydrogenase ADH IIB - Pseudomonas putida

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Q8GR64 (Q8GR64_PSEPU) Unreviewed, UniProtKB/TrEMBL

Last modified July 27, 2011. Version 51. History...

Clusters with 100%, 90%, 50% identity |

Third-party data

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Names·Attributes·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info Customize order

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Names·Attributes·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info Customize order

Names and origin

Protein names

Submitted name:
Quinohemoprotein alcohol dehydrogenase ADH IIB EMBL BAC15559.1

Gene names

Name:

qbdA EMBL BAC15559.1

Organism

Pseudomonas putida (Arthrobacter siderocapsulatus) EMBL BAC15559.1

Taxonomic identifier

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	690 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Calcium PDB 1KV9 Heme PDB 1KV9 Iron Metal-binding
Technical term	3D-structure PDB 1KV9
Gene Ontology (GO)
Cellular component	membrane Inferred from electronic annotation. Source: InterPro outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on CH-OH group of donors Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Region

3

Pyrroloquinoline quinone binding PDB 1KV9

Region

3

Pyrroloquinoline quinone binding PDB 1KV9

Region

2

Pyrroloquinoline quinone binding PDB 1KV9

Sites

Metal binding

1

Calcium PDB 1KV9

Metal binding

1

Calcium PDB 1KV9

Metal binding

1

Calcium PDB 1KV9

Metal binding

1

Iron (heme axial ligand); via tele nitrogen PDB 1KV9

Metal binding

1

Iron (heme axial ligand) PDB 1KV9

Binding site

1

Pyrroloquinoline quinone PDB 1KV9

Binding site

1

Pyrroloquinoline quinone PDB 1KV9

Binding site

1

Pyrroloquinoline quinone PDB 1KV9

Binding site

1

Pyrroloquinoline quinone PDB 1KV9

Binding site

1

Pyrroloquinoline quinone PDB 1KV9

Binding site

1

Heme (covalent) PDB 1KV9

Binding site

1

Heme (covalent) PDB 1KV9

Sequences

Sequence

Length

Mass (Da)

Tools

Q8GR64 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: F5198701D0937613
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690

74,969

        10         20         30         40         50         60 
MKKPLRTSLL MLCLATPLAA LAAGVDEAAI RATEQAGGEW LSHGRTYAEQ RFSPLKQIDA 

        70         80         90        100        110        120 
SNVRSLGLAW YMDLDNTRGL EATPLFHDGV IYTSMSWSRV IAVDAASGKE LWRYDPEVAK 

       130        140        150        160        170        180 
VKARTSCCDA VNRGVALWGD KVYVGTLDGR LIALDAKTGK AIWSQQTTDP AKPYSITGAP 

       190        200        210        220        230        240 
RVVKGKVIIG NGGAEYGVRG FVSAYDADTG KLAWRFYTVP GDPALPYEHP ELREAAKTWQ 

       250        260        270        280        290        300 
GDQYWKLGGG GTVWDSMAYD PELDLLYVGT GNGSPWNREV RSPGGGDNLY LSSILAIRPD 

       310        320        330        340        350        360 
TGKLAWHYQV TPGDSWDFTA TQQITLAELN IDGKPRKVLM QAPKNGFFYV LDRTNGKLIS 

       370        380        390        400        410        420 
AEKFGKVTWA EKVDLATGRP VEAPGVRYEK EPIVMWPSPF GAHNWHSMSF NPGTGLVYIP 

       430        440        450        460        470        480 
YQEVPGVYRN EGKDFVTRKA FNTAAGFADA TDVPAAVVSG ALLAWDPVKQ KAAWKVPYPT 

       490        500        510        520        530        540 
HWNGGTLSTA GNLVFQGTAA GQMHAYSADK GEALWQFEAQ SGIVAAPMTF ELAGRQYVAI 

       550        560        570        580        590        600 
MAGWGGVATL TGGESMNLPG MKNRSRLLVF ALDGKAQLPP PAPAPAKVER VPQPVTAAPE 

       610        620        630        640        650        660 
QVQAGKQLYG QFCSVCHGMG TISGGLIPDL RQSSDATREH FQQIVLQGAL KPLGMPSFDD 

       670        680        690 
SLKPEEVEQI KLYVMSREYE DYMARHKAAP

References

[1]	"Molecular cloning of quinohemoprotein alcohol dehydrogenase, ADH IIB, from Pseudomonas putida HK5." Toyama H., Fujii T., Aoki N., Matsushita K., Adachi O. Biosci. Biotechnol. Biochem. 67:1397-1400(2003) [PubMed: 12843671] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Structure at 1.9 A resolution of a quinohemoprotein alcohol dehydrogenase from Pseudomonas putida HK5." Chen Z.W., Matsushita K., Yamashita T., Fujii T.A., Toyama H., Adachi O., Bellamy H.D., Mathews F.S. Structure 10:837-849(2002) [PubMed: 12057198] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 23-690 IN COMPLEX WITH CALCIUM; HEME AND PYRROLOQUINOLINE QUINONE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB091400 Genomic DNA. Translation: BAC15559.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KV9	X-ray	1.90	A	23-690	[»]

ProteinModelPortal

SMR

Q8GR64. Positions 23-686.

ModBase

Protocols and materials databases

StructuralBiologyKnowledgebase

Family and domain databases

InterPro

IPR009056. Cyt_c_dom.
IPR003088. Cyt_c_I.
IPR019556. PQQ-dependent_C.
IPR019551. PQQ-dependent_N.
IPR018391. PQQ_beta_propeller_repeat.
IPR017512. PQQ_MeOH/EtOH_DH.
IPR002372. PQQ_repeat.
IPR011047. Quinonprotein_ADH-like.
IPR001479. Quinoprotein_DH_CS.
[Graphical view]

Gene3D

G3DSA:1.10.760.10. Cytochrome_c_R. 1 hit.
G3DSA:2.140.10.10. Quinoprotein_alc_DH-like. 1 hit.

Pfam

PF00034. Cytochrom_C. 1 hit.
PF01011. PQQ. 3 hits.
PF10535. PQQ_C. 1 hit.
PF10527. PQQ_N. 1 hit.
[Graphical view]

SMART

SM00564. PQQ. 5 hits.
[Graphical view]

SUPFAM

SSF46626. Cytochrome_c. 1 hit.
SSF50998. Quin_alc_DH_like. 1 hit.

TIGRFAMs

TIGR03075. PQQ_enz_alc_DH. 1 hit.

PROSITE

PS00364. BACTERIAL_PQQ_2. 1 hit.
PS51007. CYTC. 1 hit.
[Graphical view]

ProtoNet

Entry information

Entry name

Q8GR64_PSEPU

Accession

Primary (citable) accession number: Q8GR64

Entry history

Integrated into UniProtKB/TrEMBL:	March 1, 2003
Last sequence update:	March 1, 2003
Last modified:	July 27, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

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Names·Attributes·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info