Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q8GQS5 (IPYR_AQUPY)

Last modified June 16, 2009. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Inorganic pyrophosphatase
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate phospho-hydrolase
      Short name=PPase
Gene names
Name: ppa
OrganismAquifex pyrophilus
Taxonomic identifier2714 [NCBI]
Taxonomic lineageBacteriaAquificaeAquificalesAquificaceaeAquifex

Protein attributes

Sequence length177 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Catalytic activity

Diphosphate + H2O = 2 phosphate. HAMAP MF_00209

Cofactor

Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the PPase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandMagnesium
Metal-binding
   Molecular functionHydrolase
Gene Ontology (GO)
   Biological processphosphate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioninorganic diphosphatase activity

Inferred from electronic annotation. Source: HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 177177Inorganic pyrophosphatase HAMAP MF_00209
PRO_0000137476

Sites

Metal binding651Magnesium 1 By similarity
Metal binding701Magnesium 1 By similarity
Metal binding701Magnesium 2 By similarity
Metal binding1021Magnesium 1 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8GQS5-1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 3B682C5DE65AB075

FASTA17720,330
        10         20         30         40         50         60 
MGYKDLPPGK NPPEDIYVVI EIPQGSGIKY ELDKDTGVIF VDRFLFTAMY YPFNYGFIPQ 

        70         80         90        100        110        120 
TLADDGDPVD VLVISREPVA PGSVMRCRPI GMLEMRDEEG IDTKLIAVPH EKLDPTYSDI 

       130        140        150        160        170 
KTVDQLPEII RERIKHFFEH YKELEPGKWV KVENWRGLQD AIEEIKKGIE NYKKGGK 

« Hide

References

[1]"Molecular cloning, expression, and characterization of inorganic pyrophosphatase from Aquifex pyrophilus."
Hoe H.-S., Kim H.-K., Kwon S.-T.
Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

AF312862 Genomic DNA. Translation: AAN39919.1.

3D structure databases

HSSPHSSP built from PDB template 1FAJ based on UniProtKB P17288.
ModBaseSearch...

Enzyme and pathway databases

BRENDA3.6.1.1. 271525.

Family and domain databases

HAMAPMF_00209.
[Tree]
InterProIPR008162. Pyrophosphatase.
[Graphical view]
Gene3DG3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
PANTHERPTHR10286. Pyrophosphatase. 1 hit.
PfamPF00719. Pyrophosphatase. 1 hit.
[Graphical view]
ProDomPD002014. Inorg_pphsph. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS00387. PPASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIPYR_AQUPY
AccessionPrimary (citable) accession number: Q8GQS5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 11, 2003
Last sequence update: March 1, 2003
Last modified: June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents