Reviewed, UniProtKB/Swiss-Prot Q8GQS5 (IPYR_AQUPY)

Names and origin

Protein names

Recommended name:
    Inorganic pyrophosphatase
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate phospho-hydrolase
      Short name=PPase

Gene names

Name:

ppa

Organism

Aquifex pyrophilus

Taxonomic identifier

2714 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_00209
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity. HAMAP MF_00209
Subcellular location	Cytoplasm By similarity HAMAP MF_00209.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 177	177	Inorganic pyrophosphatase HAMAP MF_00209		PRO_0000137476
Sites
	Metal binding	65	1	Magnesium 1 By similarity
	Metal binding	70	1	Magnesium 1 By similarity
	Metal binding	70	1	Magnesium 2 By similarity
	Metal binding	102	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8GQS5-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 3B682C5DE65AB075	FASTA	177	20,330
	10 20 30 40 50 60 MGYKDLPPGK NPPEDIYVVI EIPQGSGIKY ELDKDTGVIF VDRFLFTAMY YPFNYGFIPQ 70 80 90 100 110 120 TLADDGDPVD VLVISREPVA PGSVMRCRPI GMLEMRDEEG IDTKLIAVPH EKLDPTYSDI 130 140 150 160 170 KTVDQLPEII RERIKHFFEH YKELEPGKWV KVENWRGLQD AIEEIKKGIE NYKKGGK

References

[1]	"Molecular cloning, expression, and characterization of inorganic pyrophosphatase from Aquifex pyrophilus." Hoe H.-S., Kim H.-K., Kwon S.-T. Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF312862 Genomic DNA. Translation: AAN39919.1.
3D structure databases
SMR	Q8GQS5. Positions 4-170.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.6.1.1. 271525.
Family and domain databases
HAMAP	MF_00209. Inorganic_PPase. [Tree]
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_AQUPY

Accession

Primary (citable) accession number: Q8GQS5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	March 1, 2003
Last modified:	January 19, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)