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Q8GQC5 (GLGB_DICCH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
OrganismDickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi)
Taxonomic identifier556 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeDickeya

Protein attributes

Sequence length731 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Biophysicochemical properties

pH dependence:

Optimum pH is 7. HAMAP-Rule MF_00685

Temperature dependence:

Optimum temperature is 30 degrees Celsius.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7317311,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188705

Sites

Active site4091Nucleophile By similarity
Active site4621Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8GQC5 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 976E2777C7FC5F0F

FASTA73183,859
        10         20         30         40         50         60 
MFVAAMTESD QNIINLLFSG HYADPFAVLG MHDTASGLEV RALLPDAIDV WVVDAHSGRK 

        70         80         90        100        110        120 
VANLQCRDPR GFFASAIPRR KKPFSYRLAV TWPQDTQVID DPYRFGTLLQ ELDIWLLAEG 

       130        140        150        160        170        180 
RHLRPFETLG AHPSTLDGVV GTCFAVWAPN AQRVSVVGDF NFWDGRRHPM RRRRENGVWE 

       190        200        210        220        230        240 
LFVPGVGPGQ LYKFEIIDCY GNVLVKSDPY AFESQMRPDT ASVVSRLPPA LPVDEARQHA 

       250        260        270        280        290        300 
NELQSPISIY EVHLGSWRRH THNNFWLSYR ELADQLVPYV KEMGFTHVEL MPVHKHPFDG 

       310        320        330        340        350        360 
SWGYQPLGLY APTRRFGSPD DFRYLVSAFH EAGINVLLDW VSGHFPADSY GLARFDGPAL 

       370        380        390        400        410        420 
YEYADPKEGY HQDWNTLIYN FDRHEVRNYL AGNALYWTER FGVDGLRVDA VASMIYRDYS 

       430        440        450        460        470        480 
RRDGEWVPNY FGGKENLEAI GFLRYTNQML GQHHAGAVTI AEESTDYAGV TLPPEHGGLG 

       490        500        510        520        530        540 
FHYKWNMGWM HDSLAYMQLD PVHRKYHHDL LTFGMLYAYS ENFVLPLSHD EVVHGKRSLL 

       550        560        570        580        590        600 
DRMPGDVWQK FANLRAYYGF MWAYPGKKLL FMGGEFAQGR EWNHDTSLDW HLLDEPEGWH 

       610        620        630        640        650        660 
AGVQQLVRDL NHCYRQHPPL YQCDYLHQGF EWVVVDDREN SVFAFIRRDA DGNEMLIISN 

       670        680        690        700        710        720 
FTPVPRDSYR VGINQPGAWR EVLNTDSWHY HGGNLGNQGL VYSETVGSHS RPQSLVLALP 

       730 
PLATLYLVKE A 

« Hide

References

[1]"Cloning and characterization of the glycogen branching enzyme gene existing in tandem with the glycogen debranching enzyme from Pectobacterium chrysanthemi PY35."
Lim W.-J., Park S.-R., Kim M.-K., An C.-L., Yun H.-J., Hong S.-Y., Kim E.-J., Shin E.-C., Lee S.-W., Lim Y.-P., Yun H.-D.
Biochem. Biophys. Res. Commun. 300:93-101(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-6, CHARACTERIZATION.
Strain: PY35.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF434710 Genomic DNA. Translation: AAN64024.1.

3D structure databases

ProteinModelPortalQ8GQC5.
SMRQ8GQC5. Positions 122-731.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_DICCH
AccessionPrimary (citable) accession number: Q8GQC5
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: March 1, 2003
Last modified: June 11, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries