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Q8GQC5

- GLGB_DICCH

UniProt

Q8GQC5 - GLGB_DICCH

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Protein
1,4-alpha-glucan branching enzyme GlgB
Gene
glgB
Organism
Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

pH dependencei

Optimum pH is 7.UniRule annotation

Temperature dependencei

Optimum temperature is 30 degrees Celsius.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei409 – 4091Nucleophile By similarity
Active sitei462 – 4621Proton donor By similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name:
BE
Gene namesi
Name:glgB
OrganismiDickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi)
Taxonomic identifieri556 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeDickeya

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7317311,4-alpha-glucan branching enzyme GlgBUniRule annotation
PRO_0000188705Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ8GQC5.
SMRiQ8GQC5. Positions 122-731.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8GQC5-1 [UniParc]FASTAAdd to Basket

« Hide

MFVAAMTESD QNIINLLFSG HYADPFAVLG MHDTASGLEV RALLPDAIDV    50
WVVDAHSGRK VANLQCRDPR GFFASAIPRR KKPFSYRLAV TWPQDTQVID 100
DPYRFGTLLQ ELDIWLLAEG RHLRPFETLG AHPSTLDGVV GTCFAVWAPN 150
AQRVSVVGDF NFWDGRRHPM RRRRENGVWE LFVPGVGPGQ LYKFEIIDCY 200
GNVLVKSDPY AFESQMRPDT ASVVSRLPPA LPVDEARQHA NELQSPISIY 250
EVHLGSWRRH THNNFWLSYR ELADQLVPYV KEMGFTHVEL MPVHKHPFDG 300
SWGYQPLGLY APTRRFGSPD DFRYLVSAFH EAGINVLLDW VSGHFPADSY 350
GLARFDGPAL YEYADPKEGY HQDWNTLIYN FDRHEVRNYL AGNALYWTER 400
FGVDGLRVDA VASMIYRDYS RRDGEWVPNY FGGKENLEAI GFLRYTNQML 450
GQHHAGAVTI AEESTDYAGV TLPPEHGGLG FHYKWNMGWM HDSLAYMQLD 500
PVHRKYHHDL LTFGMLYAYS ENFVLPLSHD EVVHGKRSLL DRMPGDVWQK 550
FANLRAYYGF MWAYPGKKLL FMGGEFAQGR EWNHDTSLDW HLLDEPEGWH 600
AGVQQLVRDL NHCYRQHPPL YQCDYLHQGF EWVVVDDREN SVFAFIRRDA 650
DGNEMLIISN FTPVPRDSYR VGINQPGAWR EVLNTDSWHY HGGNLGNQGL 700
VYSETVGSHS RPQSLVLALP PLATLYLVKE A 731
Length:731
Mass (Da):83,859
Last modified:March 1, 2003 - v1
Checksum:i976E2777C7FC5F0F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF434710 Genomic DNA. Translation: AAN64024.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF434710 Genomic DNA. Translation: AAN64024.1 .

3D structure databases

ProteinModelPortali Q8GQC5.
SMRi Q8GQC5. Positions 122-731.
ModBasei Search...

Protein family/group databases

CAZyi CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00164 .

Family and domain databases

Gene3Di 2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_00685. GlgB.
InterProi IPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view ]
PIRSFi PIRSF000463. GlgB. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of the glycogen branching enzyme gene existing in tandem with the glycogen debranching enzyme from Pectobacterium chrysanthemi PY35."
    Lim W.-J., Park S.-R., Kim M.-K., An C.-L., Yun H.-J., Hong S.-Y., Kim E.-J., Shin E.-C., Lee S.-W., Lim Y.-P., Yun H.-D.
    Biochem. Biophys. Res. Commun. 300:93-101(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-6, CHARACTERIZATION.
    Strain: PY35.

Entry informationi

Entry nameiGLGB_DICCH
AccessioniPrimary (citable) accession number: Q8GQC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: March 1, 2003
Last modified: June 11, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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