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Q8GQC5

- GLGB_DICCH

UniProt

Q8GQC5 - GLGB_DICCH

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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

pH dependencei

Optimum pH is 7.

Temperature dependencei

Optimum temperature is 30 degrees Celsius.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei409 – 4091NucleophileBy similarity
Active sitei462 – 4621Proton donorBy similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name:
BE
Gene namesi
Name:glgB
OrganismiDickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi)
Taxonomic identifieri556 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeDickeya

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7317311,4-alpha-glucan branching enzyme GlgBPRO_0000188705Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ8GQC5.
SMRiQ8GQC5. Positions 122-731.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8GQC5 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFVAAMTESD QNIINLLFSG HYADPFAVLG MHDTASGLEV RALLPDAIDV
60 70 80 90 100
WVVDAHSGRK VANLQCRDPR GFFASAIPRR KKPFSYRLAV TWPQDTQVID
110 120 130 140 150
DPYRFGTLLQ ELDIWLLAEG RHLRPFETLG AHPSTLDGVV GTCFAVWAPN
160 170 180 190 200
AQRVSVVGDF NFWDGRRHPM RRRRENGVWE LFVPGVGPGQ LYKFEIIDCY
210 220 230 240 250
GNVLVKSDPY AFESQMRPDT ASVVSRLPPA LPVDEARQHA NELQSPISIY
260 270 280 290 300
EVHLGSWRRH THNNFWLSYR ELADQLVPYV KEMGFTHVEL MPVHKHPFDG
310 320 330 340 350
SWGYQPLGLY APTRRFGSPD DFRYLVSAFH EAGINVLLDW VSGHFPADSY
360 370 380 390 400
GLARFDGPAL YEYADPKEGY HQDWNTLIYN FDRHEVRNYL AGNALYWTER
410 420 430 440 450
FGVDGLRVDA VASMIYRDYS RRDGEWVPNY FGGKENLEAI GFLRYTNQML
460 470 480 490 500
GQHHAGAVTI AEESTDYAGV TLPPEHGGLG FHYKWNMGWM HDSLAYMQLD
510 520 530 540 550
PVHRKYHHDL LTFGMLYAYS ENFVLPLSHD EVVHGKRSLL DRMPGDVWQK
560 570 580 590 600
FANLRAYYGF MWAYPGKKLL FMGGEFAQGR EWNHDTSLDW HLLDEPEGWH
610 620 630 640 650
AGVQQLVRDL NHCYRQHPPL YQCDYLHQGF EWVVVDDREN SVFAFIRRDA
660 670 680 690 700
DGNEMLIISN FTPVPRDSYR VGINQPGAWR EVLNTDSWHY HGGNLGNQGL
710 720 730
VYSETVGSHS RPQSLVLALP PLATLYLVKE A
Length:731
Mass (Da):83,859
Last modified:March 1, 2003 - v1
Checksum:i976E2777C7FC5F0F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF434710 Genomic DNA. Translation: AAN64024.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF434710 Genomic DNA. Translation: AAN64024.1 .

3D structure databases

ProteinModelPortali Q8GQC5.
SMRi Q8GQC5. Positions 122-731.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00164 .

Family and domain databases

Gene3Di 2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_00685. GlgB.
InterProi IPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view ]
PIRSFi PIRSF000463. GlgB. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of the glycogen branching enzyme gene existing in tandem with the glycogen debranching enzyme from Pectobacterium chrysanthemi PY35."
    Lim W.-J., Park S.-R., Kim M.-K., An C.-L., Yun H.-J., Hong S.-Y., Kim E.-J., Shin E.-C., Lee S.-W., Lim Y.-P., Yun H.-D.
    Biochem. Biophys. Res. Commun. 300:93-101(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-6, CHARACTERIZATION.
    Strain: PY35.

Entry informationi

Entry nameiGLGB_DICCH
AccessioniPrimary (citable) accession number: Q8GQC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: March 1, 2003
Last modified: October 1, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3