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Q8GQC5

- GLGB_DICCH

UniProt

Q8GQC5 - GLGB_DICCH

Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.

    Catalytic activityi

    Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

    pH dependencei

    Optimum pH is 7.

    Temperature dependencei

    Optimum temperature is 30 degrees Celsius.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei409 – 4091NucleophileBy similarity
    Active sitei462 – 4621Proton donorBy similarity

    GO - Molecular functioni

    1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
    2. cation binding Source: InterPro
    3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

    GO - Biological processi

    1. glycogen biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

    Enzyme and pathway databases

    UniPathwayiUPA00164.

    Protein family/group databases

    CAZyiCBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
    Alternative name(s):
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
    Alpha-(1->4)-glucan branching enzyme
    Glycogen branching enzyme
    Short name:
    BE
    Gene namesi
    Name:glgB
    OrganismiDickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi)
    Taxonomic identifieri556 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeDickeya

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7317311,4-alpha-glucan branching enzyme GlgBPRO_0000188705Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ8GQC5.
    SMRiQ8GQC5. Positions 122-731.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di2.60.40.10. 2 hits.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_00685. GlgB.
    InterProiIPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000463. GlgB. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 2 hits.
    TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8GQC5-1 [UniParc]FASTAAdd to Basket

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    MFVAAMTESD QNIINLLFSG HYADPFAVLG MHDTASGLEV RALLPDAIDV    50
    WVVDAHSGRK VANLQCRDPR GFFASAIPRR KKPFSYRLAV TWPQDTQVID 100
    DPYRFGTLLQ ELDIWLLAEG RHLRPFETLG AHPSTLDGVV GTCFAVWAPN 150
    AQRVSVVGDF NFWDGRRHPM RRRRENGVWE LFVPGVGPGQ LYKFEIIDCY 200
    GNVLVKSDPY AFESQMRPDT ASVVSRLPPA LPVDEARQHA NELQSPISIY 250
    EVHLGSWRRH THNNFWLSYR ELADQLVPYV KEMGFTHVEL MPVHKHPFDG 300
    SWGYQPLGLY APTRRFGSPD DFRYLVSAFH EAGINVLLDW VSGHFPADSY 350
    GLARFDGPAL YEYADPKEGY HQDWNTLIYN FDRHEVRNYL AGNALYWTER 400
    FGVDGLRVDA VASMIYRDYS RRDGEWVPNY FGGKENLEAI GFLRYTNQML 450
    GQHHAGAVTI AEESTDYAGV TLPPEHGGLG FHYKWNMGWM HDSLAYMQLD 500
    PVHRKYHHDL LTFGMLYAYS ENFVLPLSHD EVVHGKRSLL DRMPGDVWQK 550
    FANLRAYYGF MWAYPGKKLL FMGGEFAQGR EWNHDTSLDW HLLDEPEGWH 600
    AGVQQLVRDL NHCYRQHPPL YQCDYLHQGF EWVVVDDREN SVFAFIRRDA 650
    DGNEMLIISN FTPVPRDSYR VGINQPGAWR EVLNTDSWHY HGGNLGNQGL 700
    VYSETVGSHS RPQSLVLALP PLATLYLVKE A 731
    Length:731
    Mass (Da):83,859
    Last modified:March 1, 2003 - v1
    Checksum:i976E2777C7FC5F0F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF434710 Genomic DNA. Translation: AAN64024.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF434710 Genomic DNA. Translation: AAN64024.1 .

    3D structure databases

    ProteinModelPortali Q8GQC5.
    SMRi Q8GQC5. Positions 122-731.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00164 .

    Family and domain databases

    Gene3Di 2.60.40.10. 2 hits.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_00685. GlgB.
    InterProi IPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000463. GlgB. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 2 hits.
    TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the glycogen branching enzyme gene existing in tandem with the glycogen debranching enzyme from Pectobacterium chrysanthemi PY35."
      Lim W.-J., Park S.-R., Kim M.-K., An C.-L., Yun H.-J., Hong S.-Y., Kim E.-J., Shin E.-C., Lee S.-W., Lim Y.-P., Yun H.-D.
      Biochem. Biophys. Res. Commun. 300:93-101(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-6, CHARACTERIZATION.
      Strain: PY35.

    Entry informationi

    Entry nameiGLGB_DICCH
    AccessioniPrimary (citable) accession number: Q8GQC5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 15, 2003
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3