Alpha-amylase A - Halothermothrix orenii

Preferred order of sections

Names and origin

Protein names

Submitted name:
Alpha-amylase A EMBL AAN52525.1

Gene names

Name:

amyA EMBL AAN52525.1

Organism

Halothermothrix orenii EMBL AAN52525.1

Taxonomic identifier

31909 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Halanaerobiales › Halanaerobiaceae › Halothermothrix

Protein attributes

Sequence length	515 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Calcium PDB 1WZA Metal-binding PDB 1WZA
Technical term	3D-structure PDB 1WZA
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	catalytic activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sites

Metal binding

44

1

Calcium 1 PDB 1WZA

Metal binding

46

1

Calcium 1 PDB 1WZA

Metal binding

48

1

Calcium 1 PDB 1WZA

Metal binding

50

1

Calcium 1; via carbonyl oxygen PDB 1WZA

Metal binding

52

1

Calcium 1 PDB 1WZA

Metal binding

65

1

Calcium 2 PDB 1WZA

Metal binding

67

1

Calcium 2; via carbonyl oxygen PDB 1WZA

Metal binding

70

1

Calcium 2 PDB 1WZA

Metal binding

73

1

Calcium 2 PDB 1WZA

Sequences

Sequence

Length

Mass (Da)

Tools

Q8GPL8 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 2BF9FAA5FBD4E0C7
Show »

FASTA

515

59,888

        10         20         30         40         50         60 
MKLKRLSFFM FVTLLVFISV FPVYANDFEK HGTYYEIFVR SFYDSDGDGI GDLKGIIEKL 

        70         80         90        100        110        120 
DYLNDGDPET IADLGVNGIW LMPIFKSPSY HGYDVTDYYK INPDYGTLED FHKLVEAAHQ 

       130        140        150        160        170        180 
RGIKVIIDLP INHTSERHPW FLKASRDKNS EYRDYYVWAG PDTDTKETKL DGGRVWHYSP 

       190        200        210        220        230        240 
TGMYYGYFWS GMPDLNYNNP EVQEKVIGIA KYWLKQGVDG FRLDGAMHIF PPAQYDKNFT 

       250        260        270        280        290        300 
WWEKFRQEIE EVKPVYLVGE VWDISETVAP YFKYGFDSTF NFKLAEAVIA TAKAGFPFGF 

       310        320        330        340        350        360 
NKKAKHIYGV YDREVGFGNY IDAPFLTNHD QNRILDQLGQ DRNKARVAAS IYLTLPGNPF 

       370        380        390        400        410        420 
IYYGEEIGMR GQGPHEVIRE PFQWYNGSGE GETYWEPAMY NDGFTSVEQE EKNLDSLLNH 

       430        440        450        460        470        480 
YRRLIHFRNE NPVFYTGKIE IINGGLNVVA FRRYNDKRDL YVYHNLVNRP VKIKVASGNW 

       490        500        510 
TLLFNSGDKE ITPVEDNNKL MYTIPAYTTI VLEKE

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References

[1]	"Cloning, sequencing and expression of an alpha-amylase gene, amyA, from the thermophilic halophile Halothermothrix orenii and purification and biochemical characterization of the recombinant enzyme." Mijts B.N., Patel B.K. Microbiology 148:2343-2349(2002) [PubMed: 12177328] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Crystal structure of AmyA lacks acidic surface and provide insights into protein stability at poly-extreme condition." Sivakumar N., Li N., Tang J.W., Patel B.K., Swaminathan K. FEBS Lett. 580:2646-2652(2006) [PubMed: 16647060] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) OF 28-515 IN COMPLEX WITH CALCIUM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF442961 Genomic DNA. Translation: AAN52525.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WZA	X-ray	1.60	A	28-515	[»]

ProteinModelPortal

Q8GPL8.

SMR

Q8GPL8. Positions 28-515.

ModBase

Search...

Protein family/group databases

CAZy

GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR006048. A-amylase_b_C.
IPR015902. Alpha_amylase.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits.

PANTHER

PTHR10357. Alpha_amylase. 1 hit.

Pfam

PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]

SMART

SM00642. Aamy. 1 hit.
[Graphical view]

SUPFAM

SSF51445. Glyco_hydro_cat. 1 hit.

ProtoNet

Search...

Entry information

Entry name

Q8GPL8_9FIRM

Accession

Primary (citable) accession number: Q8GPL8

Entry history

Integrated into UniProtKB/TrEMBL:	March 1, 2003
Last sequence update:	March 1, 2003
Last modified:	May 31, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)