ID   DKE1_ACIJO              Reviewed;         153 AA.
AC   Q8GNT2;
DT   26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   20-JAN-2009, entry version 29.
DE   RecName: Full=Acetylacetone-cleaving enzyme;
DE            EC=1.13.11.50;
DE   AltName: Full=Acetylacetone dioxygenase;
DE   AltName: Full=Diketone cleaving dioxygenase;
DE   AltName: Full=Diketone cleaving enzyme;
GN   Name=dke1;
OS   Acinetobacter johnsonii.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Moraxellaceae; Acinetobacter.
OX   NCBI_TaxID=40214;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE,
RP   CHARACTERIZATION, AND MASS SPECTROMETRY.
RC   STRAIN=DSMZ 98-849;
RX   PubMed=12379146; DOI=10.1042/BJ20021047;
RA   Straganz G.D., Glieder A., Brecker L., Ribbons D.W., Steiner W.;
RT   "Acetylacetone-cleaving enzyme Dke1: a novel C-C-bond-cleaving enzyme
RT   from Acinetobacter johnsonii.";
RL   Biochem. J. 369:573-581(2003).
CC   -!- FUNCTION: Cleaves acetylacetone to equimolar amounts of
CC       methylglyoxal and acetate, consuming one equivalent of molecular
CC       oxygen.
CC   -!- CATALYTIC ACTIVITY: Pentane-2,4-dione + O(2) = acetate + 2-
CC       oxopropanal.
CC   -!- COFACTOR: Binds 1 iron ion per subunit.
CC   -!- PATHWAY: Xenobiotic degradation; acetylacetone degradation.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- MASS SPECTROMETRY: Mass=16607; Method=MALDI; Range=1-153;
CC       Source=PubMed:12379146;
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DR   EMBL; AF489107; AAN45859.1; -; Genomic_DNA.
DR   PDB; 3BAL; X-ray; 1.95 A; A/B/C/D=1-153.
DR   PDBsum; 3BAL; -.
DR   BRENDA; 1.13.11.50; 74504.
DR   GO; GO:0033752; F:acetylacetone-cleaving enzyme activity; IEA:EC.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   3D-structure; Dioxygenase; Direct protein sequencing; Iron;
KW   Metal-binding; Oxidoreductase.
FT   CHAIN         1    153       Acetylacetone-cleaving enzyme.
FT                                /FTId=PRO_0000079925.
FT   STRAND       12     15
FT   HELIX        18     20
FT   HELIX        26     28
FT   STRAND       29     31
FT   STRAND       34     41
FT   TURN         42     45
FT   STRAND       46     53
FT   STRAND       57     59
FT   STRAND       62     66
FT   STRAND       68     79
FT   HELIX        83     85
FT   STRAND       87     98
FT   STRAND      103    105
FT   STRAND      108    111
FT   STRAND      113    121
FT   STRAND      123    126
FT   STRAND      132    136
FT   HELIX       138    148
SQ   SEQUENCE   153 AA;  16607 MW;  F3FBCB762E8F250F CRC64;
     MDYCNKKHTA EEYVKISDNN YVPFPEAFSD GGITWQLLHS SPETSSWTAI FNCPAGSSFA
     SHIHAGPGEY FLTKGKMEVR GGEQEGGSTA YAPSYGFESS GALHGKTFFP VESQFYMTFL
     GPLNFIDDNG KVIASIGWAE AQGAWLATKN EAA
//