Reviewed, UniProtKB/Swiss-Prot Q8GNT2 (DKE1_ACIJO)

Names and origin

Protein names

Recommended name:
    Acetylacetone-cleaving enzyme
    EC=1.13.11.50
Alternative name(s):
    Acetylacetone dioxygenase
    Diketone cleaving dioxygenase
    Diketone cleaving enzyme

Gene names

Name:

dke1

Organism

Acinetobacter johnsonii

Taxonomic identifier

40214 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	153 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cleaves acetylacetone to equimolar amounts of methylglyoxal and acetate, consuming one equivalent of molecular oxygen.
Catalytic activity	Pentane-2,4-dione + O2 = acetate + 2-oxopropanal.
Cofactor	Binds 1 iron ion per subunit.
Pathway	Xenobiotic degradation; acetylacetone degradation.
Subunit structure	Homotetramer.
Mass spectrometry	Molecular mass is 16607 Da from positions 1 - 153. Determined by MALDI. Ref.1

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acetylacetone-cleaving enzyme activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 153

153

Acetylacetone-cleaving enzyme

PRO_0000079925

Secondary structure

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153

Helix Strand Turn

Details...

Beta strand

12 – 15

4

Helix

18 – 20

3

Helix

26 – 28

3

Beta strand

29 – 31

3

Beta strand

34 – 41

8

Turn

42 – 45

4

Beta strand

46 – 53

8

Beta strand

57 – 59

3

Beta strand

62 – 66

5

Beta strand

68 – 79

12

Helix

83 – 85

3

Beta strand

87 – 98

12

Beta strand

103 – 105

3

Beta strand

108 – 111

4

Beta strand

113 – 121

9

Beta strand

123 – 126

4

Beta strand

132 – 136

5

Helix

138 – 148

11

Sequences

	Sequence		Length	Mass (Da)
	Q8GNT2-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: F3FBCB762E8F250F	FASTA	153	16,607
	10 20 30 40 50 60 MDYCNKKHTA EEYVKISDNN YVPFPEAFSD GGITWQLLHS SPETSSWTAI FNCPAGSSFA 70 80 90 100 110 120 SHIHAGPGEY FLTKGKMEVR GGEQEGGSTA YAPSYGFESS GALHGKTFFP VESQFYMTFL 130 140 150 GPLNFIDDNG KVIASIGWAE AQGAWLATKN EAA

References

[1]

"Acetylacetone-cleaving enzyme Dke1: a novel C-C-bond-cleaving enzyme from Acinetobacter johnsonii." Straganz G.D., Glieder A., Brecker L., Ribbons D.W., Steiner W. Biochem. J. 369:573-581(2003) [PubMed: 12379146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION, MASS SPECTROMETRY. Strain: DSMZ 98-849.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF489107 Genomic DNA. Translation: AAN45859.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3BAL	X-ray	1.95	A/B/C/D	1-153	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

1.13.11.50. 74504.

Family and domain databases

ProtoNet

Search...

Entry information

Entry name

DKE1_ACIJO

Accession

Primary (citable) accession number: Q8GNT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2003
Last sequence update:	March 1, 2003
Last modified:	January 20, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)