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Q8GNC1 (K6PF_HAEDU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
6-phosphofructokinase

Short name=Phosphofructokinase
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase
Gene names
Name:pfkA
Ordered Locus Names:HD_0465
OrganismHaemophilus ducreyi (strain 35000HP / ATCC 700724) [Complete proteome] [HAMAP]
Taxonomic identifier233412 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00339.

Sequence similarities

Belongs to the phosphofructokinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfructose 6-phosphate metabolic process

Inferred from electronic annotation. Source: InterPro

glycolysis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_component6-phosphofructokinase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_function6-phosphofructokinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3243246-phosphofructokinase HAMAP-Rule MF_00339
PRO_0000111955

Regions

Nucleotide binding25 – 295ATP By similarity
Nucleotide binding159 – 1635ATP By similarity
Nucleotide binding176 – 19217ATP By similarity

Sites

Active site1321Proton acceptor By similarity
Metal binding1901Magnesium; via carbonyl oxygen By similarity
Metal binding1921Magnesium By similarity
Binding site1671Substrate By similarity
Binding site2481Substrate By similarity
Binding site2541Substrate By similarity
Binding site2571Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8GNC1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 0F9BD638185E48BE

FASTA32435,460
        10         20         30         40         50         60 
MAKEIKKIAV LTSGGDAPGM NAAIRGVVRA ALNEGLEIFG VQDGYYGLYH DKVIPLDRRS 

        70         80         90        100        110        120 
VSEIINRGGT FLGSARFPQF KDIEVRKQAV KILAKYEIDA LVVIGGDGSY MGAKLLTEEF 

       130        140        150        160        170        180 
GYPCIGLPGT IDNDIVGTDY TIGYQTALQT AVEAIDRLRD TSTSHQRISI VEIMGRHCGD 

       190        200        210        220        230        240 
LTISAALAGG CEYIIVPEKG LDKESLIHSI ADNFRKGKRH AIIAITELMT DVHQLARELE 

       250        260        270        280        290        300 
EKFGHETRAT VLGHIQRGGS PCPFDRILAS RMGVYAVDLL RQGFGGRCVG IQNEHLVHHD 

       310        320 
IIDAINNMRR PFKQDIFEAA SKLA 

« Hide

References

« Hide 'large scale' references
[1]"Identification and characterization of the N-acetylglucosamine glycosyltransferase gene of Haemophilus ducreyi."
Sun S., Scheffler N.K., Gibson B.W., Wang J., Munson R.S. Jr.
Infect. Immun. 70:5887-5892(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 35000HP / ATCC 700724.
[2]"The complete genome sequence of Haemophilus ducreyi."
Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L.
Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 35000HP / ATCC 700724.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF536817 Genomic DNA. Translation: AAN05637.1.
AE017143 Genomic DNA. Translation: AAP95425.1.
RefSeqNP_873036.1. NC_002940.2.

3D structure databases

ProteinModelPortalQ8GNC1.
SMRQ8GNC1. Positions 5-323.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING233412.HD0465.

Proteomic databases

PRIDEQ8GNC1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAP95425; AAP95425; HD_0465.
GeneID1490458.
KEGGhdu:HD0465.
PATRIC20177453. VBIHaeDuc133973_0382.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0205.
KOK00850.
OMAKMVHHDI.
OrthoDBEOG644ZRM.
ProtClustDBPRK03202.

Enzyme and pathway databases

BioCycHDUC233412:GH5F-436-MONOMER.
UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_00339. Phosphofructokinase.
InterProIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
TIGRFAMsTIGR02482. PFKA_ATP. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameK6PF_HAEDU
AccessionPrimary (citable) accession number: Q8GNC1
Secondary accession number(s): Q7BY74
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways