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Q8GNC1 (PFKA_HAEDU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase

Short name=ATP-PFK
Short name=Phosphofructokinase
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase
Gene names
Name:pfkA
Ordered Locus Names:HD_0465
OrganismHaemophilus ducreyi (strain 35000HP / ATCC 700724) [Complete proteome] [HAMAP]
Taxonomic identifier233412 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity. HAMAP-Rule MF_00339

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00339

Enzyme regulation

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity. HAMAP-Rule MF_00339

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00339

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00339.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 324324ATP-dependent 6-phosphofructokinase HAMAP-Rule MF_00339
PRO_0000111955

Regions

Nucleotide binding76 – 772ATP By similarity
Nucleotide binding106 – 1094ATP By similarity
Region25 – 295Allosteric activator ADP binding; shared with dimeric partner By similarity
Region130 – 1323Substrate binding By similarity
Region174 – 1763Substrate binding By similarity
Region190 – 1923Allosteric activator ADP binding By similarity
Region218 – 2203Allosteric activator ADP binding By similarity
Region254 – 2574Substrate binding By similarity

Sites

Active site1321Proton acceptor By similarity
Metal binding1071Magnesium; catalytic By similarity
Binding site151ATP; via amide nitrogen By similarity
Binding site1591Allosteric activator ADP By similarity
Binding site1671Substrate; shared with dimeric partner By similarity
Binding site2161Allosteric activator ADP By similarity
Binding site2271Substrate By similarity
Binding site2481Substrate; shared with dimeric partner By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8GNC1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 0F9BD638185E48BE

FASTA32435,460
        10         20         30         40         50         60 
MAKEIKKIAV LTSGGDAPGM NAAIRGVVRA ALNEGLEIFG VQDGYYGLYH DKVIPLDRRS 

        70         80         90        100        110        120 
VSEIINRGGT FLGSARFPQF KDIEVRKQAV KILAKYEIDA LVVIGGDGSY MGAKLLTEEF 

       130        140        150        160        170        180 
GYPCIGLPGT IDNDIVGTDY TIGYQTALQT AVEAIDRLRD TSTSHQRISI VEIMGRHCGD 

       190        200        210        220        230        240 
LTISAALAGG CEYIIVPEKG LDKESLIHSI ADNFRKGKRH AIIAITELMT DVHQLARELE 

       250        260        270        280        290        300 
EKFGHETRAT VLGHIQRGGS PCPFDRILAS RMGVYAVDLL RQGFGGRCVG IQNEHLVHHD 

       310        320 
IIDAINNMRR PFKQDIFEAA SKLA 

« Hide

References

« Hide 'large scale' references
[1]"Identification and characterization of the N-acetylglucosamine glycosyltransferase gene of Haemophilus ducreyi."
Sun S., Scheffler N.K., Gibson B.W., Wang J., Munson R.S. Jr.
Infect. Immun. 70:5887-5892(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 35000HP / ATCC 700724.
[2]"The complete genome sequence of Haemophilus ducreyi."
Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L.
Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 35000HP / ATCC 700724.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF536817 Genomic DNA. Translation: AAN05637.1.
AE017143 Genomic DNA. Translation: AAP95425.1.
RefSeqNP_873036.1. NC_002940.2.

3D structure databases

ProteinModelPortalQ8GNC1.
SMRQ8GNC1. Positions 5-323.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING233412.HD0465.

Proteomic databases

PRIDEQ8GNC1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAP95425; AAP95425; HD_0465.
GeneID1490458.
KEGGhdu:HD0465.
PATRIC20177453. VBIHaeDuc133973_0382.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0205.
KOK00850.
OMAGFGGRCV.
OrthoDBEOG644ZRM.

Enzyme and pathway databases

BioCycHDUC233412:GH5F-436-MONOMER.
UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_00339. Phosphofructokinase_I_B1.
InterProIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
TIGRFAMsTIGR02482. PFKA_ATP. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePFKA_HAEDU
AccessionPrimary (citable) accession number: Q8GNC1
Secondary accession number(s): Q7BY74
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways