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Q8GNC1

- PFKA_HAEDU

UniProt

Q8GNC1 - PFKA_HAEDU

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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Haemophilus ducreyi (strain 35000HP / ATCC 700724)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151ATP; via amide nitrogenUniRule annotation
Metal bindingi107 – 1071Magnesium; catalyticUniRule annotation
Active sitei132 – 1321Proton acceptorUniRule annotation
Binding sitei159 – 1591Allosteric activator ADPUniRule annotation
Binding sitei167 – 1671Substrate; shared with dimeric partnerUniRule annotation
Binding sitei216 – 2161Allosteric activator ADPUniRule annotation
Binding sitei227 – 2271SubstrateUniRule annotation
Binding sitei248 – 2481Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi76 – 772ATPUniRule annotation
Nucleotide bindingi106 – 1094ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHDUC233412:GH5F-436-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:HD_0465
OrganismiHaemophilus ducreyi (strain 35000HP / ATCC 700724)
Taxonomic identifieri233412 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000001022: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 324324ATP-dependent 6-phosphofructokinasePRO_0000111955Add
BLAST

Proteomic databases

PRIDEiQ8GNC1.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi233412.HD0465.

Structurei

3D structure databases

ProteinModelPortaliQ8GNC1.
SMRiQ8GNC1. Positions 5-323.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 295Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
Regioni130 – 1323Substrate bindingUniRule annotation
Regioni174 – 1763Substrate bindingUniRule annotation
Regioni190 – 1923Allosteric activator ADP bindingUniRule annotation
Regioni218 – 2203Allosteric activator ADP bindingUniRule annotation
Regioni254 – 2574Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
KOiK00850.
OMAiGFGGRCV.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8GNC1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKEIKKIAV LTSGGDAPGM NAAIRGVVRA ALNEGLEIFG VQDGYYGLYH
60 70 80 90 100
DKVIPLDRRS VSEIINRGGT FLGSARFPQF KDIEVRKQAV KILAKYEIDA
110 120 130 140 150
LVVIGGDGSY MGAKLLTEEF GYPCIGLPGT IDNDIVGTDY TIGYQTALQT
160 170 180 190 200
AVEAIDRLRD TSTSHQRISI VEIMGRHCGD LTISAALAGG CEYIIVPEKG
210 220 230 240 250
LDKESLIHSI ADNFRKGKRH AIIAITELMT DVHQLARELE EKFGHETRAT
260 270 280 290 300
VLGHIQRGGS PCPFDRILAS RMGVYAVDLL RQGFGGRCVG IQNEHLVHHD
310 320
IIDAINNMRR PFKQDIFEAA SKLA
Length:324
Mass (Da):35,460
Last modified:March 1, 2003 - v1
Checksum:i0F9BD638185E48BE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF536817 Genomic DNA. Translation: AAN05637.1.
AE017143 Genomic DNA. Translation: AAP95425.1.
RefSeqiNP_873036.1. NC_002940.2.
WP_010944478.1. NC_002940.2.

Genome annotation databases

EnsemblBacteriaiAAP95425; AAP95425; HD_0465.
GeneIDi1490458.
KEGGihdu:HD0465.
PATRICi20177453. VBIHaeDuc133973_0382.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF536817 Genomic DNA. Translation: AAN05637.1 .
AE017143 Genomic DNA. Translation: AAP95425.1 .
RefSeqi NP_873036.1. NC_002940.2.
WP_010944478.1. NC_002940.2.

3D structure databases

ProteinModelPortali Q8GNC1.
SMRi Q8GNC1. Positions 5-323.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 233412.HD0465.

Proteomic databases

PRIDEi Q8GNC1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAP95425 ; AAP95425 ; HD_0465 .
GeneIDi 1490458.
KEGGi hdu:HD0465.
PATRICi 20177453. VBIHaeDuc133973_0382.

Phylogenomic databases

eggNOGi COG0205.
KOi K00850.
OMAi GFGGRCV.
OrthoDBi EOG644ZRM.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci HDUC233412:GH5F-436-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase_I_B1.
InterProi IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of the N-acetylglucosamine glycosyltransferase gene of Haemophilus ducreyi."
    Sun S., Scheffler N.K., Gibson B.W., Wang J., Munson R.S. Jr.
    Infect. Immun. 70:5887-5892(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 35000HP / ATCC 700724.
  2. "The complete genome sequence of Haemophilus ducreyi."
    Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L.
    Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 35000HP / ATCC 700724.

Entry informationi

Entry nameiPFKA_HAEDU
AccessioniPrimary (citable) accession number: Q8GNC1
Secondary accession number(s): Q7BY74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: March 1, 2003
Last modified: October 29, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3