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Q8GNC1

- PFKA_HAEDU

UniProt

Q8GNC1 - PFKA_HAEDU

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Haemophilus ducreyi (strain 35000HP / ATCC 700724)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei15 – 151ATP; via amide nitrogenUniRule annotation
    Metal bindingi107 – 1071Magnesium; catalyticUniRule annotation
    Active sitei132 – 1321Proton acceptorUniRule annotation
    Binding sitei159 – 1591Allosteric activator ADPUniRule annotation
    Binding sitei167 – 1671Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei216 – 2161Allosteric activator ADPUniRule annotation
    Binding sitei227 – 2271SubstrateUniRule annotation
    Binding sitei248 – 2481Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi76 – 772ATPUniRule annotation
    Nucleotide bindingi106 – 1094ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciHDUC233412:GH5F-436-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Ordered Locus Names:HD_0465
    OrganismiHaemophilus ducreyi (strain 35000HP / ATCC 700724)
    Taxonomic identifieri233412 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000001022: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 324324ATP-dependent 6-phosphofructokinasePRO_0000111955Add
    BLAST

    Proteomic databases

    PRIDEiQ8GNC1.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi233412.HD0465.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8GNC1.
    SMRiQ8GNC1. Positions 5-323.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni25 – 295Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni130 – 1323Substrate bindingUniRule annotation
    Regioni174 – 1763Substrate bindingUniRule annotation
    Regioni190 – 1923Allosteric activator ADP bindingUniRule annotation
    Regioni218 – 2203Allosteric activator ADP bindingUniRule annotation
    Regioni254 – 2574Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    KOiK00850.
    OMAiGFGGRCV.
    OrthoDBiEOG644ZRM.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase_I_B1.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8GNC1-1 [UniParc]FASTAAdd to Basket

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    MAKEIKKIAV LTSGGDAPGM NAAIRGVVRA ALNEGLEIFG VQDGYYGLYH    50
    DKVIPLDRRS VSEIINRGGT FLGSARFPQF KDIEVRKQAV KILAKYEIDA 100
    LVVIGGDGSY MGAKLLTEEF GYPCIGLPGT IDNDIVGTDY TIGYQTALQT 150
    AVEAIDRLRD TSTSHQRISI VEIMGRHCGD LTISAALAGG CEYIIVPEKG 200
    LDKESLIHSI ADNFRKGKRH AIIAITELMT DVHQLARELE EKFGHETRAT 250
    VLGHIQRGGS PCPFDRILAS RMGVYAVDLL RQGFGGRCVG IQNEHLVHHD 300
    IIDAINNMRR PFKQDIFEAA SKLA 324
    Length:324
    Mass (Da):35,460
    Last modified:March 1, 2003 - v1
    Checksum:i0F9BD638185E48BE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF536817 Genomic DNA. Translation: AAN05637.1.
    AE017143 Genomic DNA. Translation: AAP95425.1.
    RefSeqiNP_873036.1. NC_002940.2.
    WP_010944478.1. NC_002940.2.

    Genome annotation databases

    EnsemblBacteriaiAAP95425; AAP95425; HD_0465.
    GeneIDi1490458.
    KEGGihdu:HD0465.
    PATRICi20177453. VBIHaeDuc133973_0382.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF536817 Genomic DNA. Translation: AAN05637.1 .
    AE017143 Genomic DNA. Translation: AAP95425.1 .
    RefSeqi NP_873036.1. NC_002940.2.
    WP_010944478.1. NC_002940.2.

    3D structure databases

    ProteinModelPortali Q8GNC1.
    SMRi Q8GNC1. Positions 5-323.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 233412.HD0465.

    Proteomic databases

    PRIDEi Q8GNC1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAP95425 ; AAP95425 ; HD_0465 .
    GeneIDi 1490458.
    KEGGi hdu:HD0465.
    PATRICi 20177453. VBIHaeDuc133973_0382.

    Phylogenomic databases

    eggNOGi COG0205.
    KOi K00850.
    OMAi GFGGRCV.
    OrthoDBi EOG644ZRM.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci HDUC233412:GH5F-436-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase_I_B1.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of the N-acetylglucosamine glycosyltransferase gene of Haemophilus ducreyi."
      Sun S., Scheffler N.K., Gibson B.W., Wang J., Munson R.S. Jr.
      Infect. Immun. 70:5887-5892(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 35000HP / ATCC 700724.
    2. "The complete genome sequence of Haemophilus ducreyi."
      Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L.
      Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 35000HP / ATCC 700724.

    Entry informationi

    Entry nameiPFKA_HAEDU
    AccessioniPrimary (citable) accession number: Q8GNC1
    Secondary accession number(s): Q7BY74
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2005
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3