Q8GN86 (Q8GN86_9BURK) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Submitted name: 4-chlorobenzoyl CoA ligase EMBL AAN10109.1
Encoded on	Plasmid pss70 EMBL AAN10109.1
Organism	Alcaligenes sp. AL3007 EMBL AAN10109.1
Taxonomic identifier	206162 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Alcaligenes

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Molecular function	Ligase EMBL AAN10109.1
Technical term	3D-structure PDB 2QVZ PDB 2QVY PDB 2QVX PDB 1T5H PDB 2QW0 PDB 3CW8 PDB 3CW9 PDB 3DLP PDB 1T5D Plasmid EMBL AAN10109.1
Gene Ontology (GO)
Molecular function	ligase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q8GN86 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 3D6E2B678EBE4F3F	FASTA	501	53,930
	10 20 30 40 50 60 MQTVNEMLRR AATRAPDHCA LAVPARGLRL THAELRARVE AVAGLHADGL RPQQRVAVVA 70 80 90 100 110 120 PNSADVVIAI LALHRLAVPA LLNPRLKSAE LAELIKRGEM TAAVIAVGRQ VADAIFQSGS 130 140 150 160 170 180 GARIIFLGDL VRDGEPYSYG PPIEDPQRDA AQPAFIFYTS GTTGLPKAAI IPQRAAESRV 190 200 210 220 230 240 LFMSTQVGLR HGRHNVVLGL MPLYHVVGFF AVLVAALALD GTYVVVEEFR PVDALQLVQQ 250 260 270 280 290 300 EQVTSLFATP THLDALAAAA HAGSSLKLDS LRHVTFAGAT MPDAVLETVH QHLPGEKVNI 310 320 330 340 350 360 YGTTEAMNSL YMRHAKTGTE MAPGFFSEVR IVRIGGGVDE IVANGEEGEL IVAASDSAFV 370 380 390 400 410 420 GYLNQPQATA EKLQDGWYRT SDVAVWTPEG TVRILGRVDD MIISGGENIH PSEIERVLGT 430 440 450 460 470 480 APGVAEVVVI GLADQRWGQS VTACVVPRLG ETLSADALDT FCRSSELADF KRPKRYFILD 490 500 QLPKNALNKV LRRHVVQQVS S

References

[1]	"Characterization of the 4-hydroxybenzoyl-coenzyme A thioesterase from Arthrobacter sp. strain SU." Zhuang Z., Gartemann K.H., Eichenlaub R., Dunaway-Mariano D. Appl. Environ. Microbiol. 69:2707-2711(2003) [PubMed: 12732540] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: AL3007 EMBL AAN10109.1.
[2]	Lai S., Zhang W., Dunaway-Mariano D. Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: AL3007 EMBL AAN10109.1.
[3]	"Crystal structure of 4-chlorobenzoate:CoA ligase/synthetase in the unliganded and aryl substrate-bound states." Gulick A.M., Lu X., Dunaway-Mariano D. Biochemistry 43:8670-8679(2004) [PubMed: 15236575] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS).
[4]	"Rational redesign of the 4-chlorobenzoate binding site of 4-chlorobenzoate: coenzyme a ligase for expanded substrate range." Wu R., Reger A.S., Cao J., Gulick A.M., Dunaway-Mariano D. Biochemistry 46:14487-14499(2007) [PubMed: 18027984] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS).
[5]	"Structural characterization of a 140 degrees domain movement in the two-step reaction catalyzed by 4-chlorobenzoate:CoA ligase." Reger A.S., Wu R., Dunaway-Mariano D., Gulick A.M. Biochemistry 47:8016-8025(2008) [PubMed: 18620418] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS).
[6]	"The mechanism of domain alternation in the acyl-adenylate forming ligase superfamily member 4-chlorobenzoate: coenzyme A ligase." Wu R., Reger A.S., Lu X., Gulick A.M., Dunaway-Mariano D. Biochemistry 48:4115-4125(2009) [PubMed: 19320426] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF537222 Genomic DNA. Translation: AAN10109.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1T5D	X-ray	2.21	X	1-501	[»]
1T5H	X-ray	2.00	X	1-501	[»]
2QVX	X-ray	2.70	X	1-501	[»]
2QVY	X-ray	2.76	X	1-501	[»]
2QVZ	X-ray	2.50	X	1-501	[»]
2QW0	X-ray	2.56	X	1-501	[»]
3CW8	X-ray	2.25	X	1-501	[»]
3CW9	X-ray	2.00	A/B	1-501	[»]
3DLP	X-ray	2.60	X	1-501	[»]

ProteinModelPortal

Q8GN86.

SMR

Q8GN86. Positions 1-501.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

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Family and domain databases

InterPro

IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]

Pfam

PF00501. AMP-binding. 1 hit.
[Graphical view]

PROSITE

PS00455. AMP_BINDING. 1 hit.
[Graphical view]

ProtoNet

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Entry information

Entry name

Q8GN86_9BURK

Accession

Primary (citable) accession number: Q8GN86

Entry history

Integrated into UniProtKB/TrEMBL:	March 1, 2003
Last sequence update:	March 1, 2003
Last modified:	January 11, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)