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Q8GJP4 (FOLKE_LACLM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Bifunctional protein FolKE

Including the following 2 domains:

  1. 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase
    EC=2.7.6.3
    Alternative name(s):
    6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
    Short name=PPPK
    7,8 dihydro-6-hydroxymethylpterin-pyrophosphokinase
    Short name=HPPK
  2. GTP cyclohydrolase 1
    EC=3.5.4.16
    Alternative name(s):
    GTP cyclohydrolase I
    Short name=GTP-CH-I
Gene names
Name:folKE
Synonyms:folE
Ordered Locus Names:llmg_1337
OrganismLactococcus lactis subsp. cremoris (strain MG1363) [Complete proteome] [HAMAP]
Taxonomic identifier416870 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus ›

Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine = AMP + (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate. HAMAP-Rule MF_00223

GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate. HAMAP-Rule MF_00223

Pathway

Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1. HAMAP-Rule MF_00223

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 4/4. HAMAP-Rule MF_00223

Subunit structure

Homopolymer By similarity. HAMAP-Rule MF_00223

Sequence similarities

In the N-terminal section; belongs to the HPPK family.

In the C-terminal section; belongs to the GTP cyclohydrolase I family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 349349Bifunctional protein FolKE HAMAP-Rule MF_00223
PRO_0000119472

Regions

Region1 – 2262262-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase HAMAP-Rule MF_00223
Region226 – 349124GTP cyclohydrolase 1 HAMAP-Rule MF_00223

Sequences

Sequence LengthMass (Da)Tools
Q8GJP4 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 105B058C80E3A8B4

FASTA34940,261
        10         20         30         40         50         60 
MQTTYLSMGS NIGDRQYYLH EAIRLLGKHP KIMIEKVSNF YESSPVGGVK QDDFTNLALK 

        70         80         90        100        110        120 
VATLLEPLEL LDFIHEVELS LNRERKIHWG PRTIDIDIIF YGNSEIQEEN LIVPHKEAFN 

       130        140        150        160        170        180 
RLFVLKPIFE LLSNDFKYYE PIREAIAKLS ESEQELHVIE EEKSPKNRIE EAVKEILFAV 

       190        200        210        220        230        240 
GENPNREGLL ETPARVAKMY EEILSSQRLT NFNEYKLFEI DSSKNDSIVL IKDIPFYSMC 

       250        260        270        280        290        300 
EHHMLPFFGK AHVAYIPDGG RIIGLSKIPR LVNYVSRKLS VQENITHDIA DILTDILKPK 

       310        320        330        340 
GVAVLVEGRH MCVEMRGVKK VNSLTKTSYF LGEFKENGEK RMEFLESLL

« Hide

References

« Hide 'large scale' references
[1]"Increased production of folate by metabolic engineering of Lactococcus lactis."
Sybesma W., Starrenburg M., Kleerebezem M., Mierau I., de Vos W.M., Hugenholtz J.
Appl. Environ. Microbiol. 69:3069-3076(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363."
Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J.
J. Bacteriol. 189:3256-3270(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MG1363.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY156932 Genomic DNA. Translation: AAN64306.1.
AM406671 Genomic DNA. Translation: CAL97928.1.
RefSeqYP_001032640.1. NC_009004.1.

3D structure databases

ProteinModelPortalQ8GJP4.
ModBaseSearch...

Protein-protein interaction databases

STRING416870.llmg_1337.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL97928; CAL97928; llmg_1337.
GeneID4797323.
KEGGllm:llmg_1337.
PATRIC22283849. VBILacLac4574_1380.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0801.
HOGENOMHOG000022731.
KOK00950.
K01495.
OMAFIHEVEL.
ProtClustDBCLSK876844.

Enzyme and pathway databases

BioCycLLAC416870:GCDT-1361-MONOMER.
UniPathwayUPA00077; UER00155.
UPA00848; UER00151.

Family and domain databases

Gene3D3.30.70.560. 1 hit.
HAMAPMF_00223. FolE. Fused.
InterProIPR001474. GTP_CycHdrlase_I.
IPR020602. GTP_CycHdrlase_I/CN_OxRdtase.
IPR018234. GTP_CycHdrlase_I_CS.
IPR000550. Hppk.
[Graphical view]
PANTHERPTHR11109. PTHR11109. 1 hit.
PfamPF01227. GTP_cyclohydroI. 1 hit.
PF01288. HPPK. 1 hit.
[Graphical view]
SUPFAMSSF55083. Hppk. 1 hit.
TIGRFAMsTIGR00063. folE. 1 hit.
TIGR01498. folK. 1 hit.
PROSITEPS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
PS00860. GTP_CYCLOHYDROL_1_2. False negative.
PS00794. HPPK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFOLKE_LACLM
AccessionPrimary (citable) accession number: Q8GJP4
Secondary accession number(s): A2RKW2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: March 1, 2003
Last modified: May 29, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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