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Q8GJP4

- FOLKE_LACLM

UniProt

Q8GJP4 - FOLKE_LACLM

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Protein

Bifunctional protein FolKE

Gene

folKE

Organism
Lactococcus lactis subsp. cremoris (strain MG1363)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine = AMP + (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate.
GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate.

Pathwayi

GO - Molecular functioni

  1. 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. GTP binding Source: UniProtKB-KW
  4. GTP cyclohydrolase I activity Source: UniProtKB-HAMAP
  5. kinase activity Source: UniProtKB-KW
  6. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. 7,8-dihydroneopterin 3'-triphosphate biosynthetic process Source: UniProtKB-UniPathway
  2. folic acid biosynthetic process Source: UniProtKB-KW
  3. one-carbon metabolic process Source: UniProtKB-HAMAP
  4. tetrahydrofolate biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Transferase

Keywords - Biological processi

Folate biosynthesis, One-carbon metabolism

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLLAC416870:GCDT-1361-MONOMER.
UniPathwayiUPA00077; UER00155.
UPA00848; UER00151.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein FolKE
Including the following 2 domains:
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (EC:2.7.6.3)
Alternative name(s):
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
Short name:
PPPK
7,8 dihydro-6-hydroxymethylpterin-pyrophosphokinase
Short name:
HPPK
GTP cyclohydrolase 1 (EC:3.5.4.16)
Alternative name(s):
GTP cyclohydrolase I
Short name:
GTP-CH-I
Gene namesi
Name:folKE
Synonyms:folE
Ordered Locus Names:llmg_1337
OrganismiLactococcus lactis subsp. cremoris (strain MG1363)
Taxonomic identifieri416870 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
ProteomesiUP000000364: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 349349Bifunctional protein FolKEPRO_0000119472Add
BLAST

Interactioni

Subunit structurei

Homopolymer.By similarity

Protein-protein interaction databases

STRINGi416870.llmg_1337.

Structurei

3D structure databases

ProteinModelPortaliQ8GJP4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2262262-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinaseAdd
BLAST
Regioni226 – 349124GTP cyclohydrolase 1Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the HPPK family.Curated
In the C-terminal section; belongs to the GTP cyclohydrolase I family.Curated

Phylogenomic databases

eggNOGiCOG0801.
HOGENOMiHOG000022731.
KOiK00950.
K01495.
OMAiFIHEVEL.
OrthoDBiEOG6XHC8G.

Family and domain databases

Gene3Di3.30.70.560. 1 hit.
HAMAPiMF_00223. FolE.
InterProiIPR001474. GTP_CycHdrlase_I.
IPR018234. GTP_CycHdrlase_I_CS.
IPR020602. GTP_CycHdrlase_I_dom.
IPR000550. Hppk.
[Graphical view]
PANTHERiPTHR11109. PTHR11109. 1 hit.
PfamiPF01227. GTP_cyclohydroI. 1 hit.
PF01288. HPPK. 1 hit.
[Graphical view]
SUPFAMiSSF55083. SSF55083. 1 hit.
TIGRFAMsiTIGR00063. folE. 1 hit.
TIGR01498. folK. 1 hit.
PROSITEiPS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
PS00794. HPPK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8GJP4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQTTYLSMGS NIGDRQYYLH EAIRLLGKHP KIMIEKVSNF YESSPVGGVK
60 70 80 90 100
QDDFTNLALK VATLLEPLEL LDFIHEVELS LNRERKIHWG PRTIDIDIIF
110 120 130 140 150
YGNSEIQEEN LIVPHKEAFN RLFVLKPIFE LLSNDFKYYE PIREAIAKLS
160 170 180 190 200
ESEQELHVIE EEKSPKNRIE EAVKEILFAV GENPNREGLL ETPARVAKMY
210 220 230 240 250
EEILSSQRLT NFNEYKLFEI DSSKNDSIVL IKDIPFYSMC EHHMLPFFGK
260 270 280 290 300
AHVAYIPDGG RIIGLSKIPR LVNYVSRKLS VQENITHDIA DILTDILKPK
310 320 330 340
GVAVLVEGRH MCVEMRGVKK VNSLTKTSYF LGEFKENGEK RMEFLESLL
Length:349
Mass (Da):40,261
Last modified:March 1, 2003 - v1
Checksum:i105B058C80E3A8B4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY156932 Genomic DNA. Translation: AAN64306.1.
AM406671 Genomic DNA. Translation: CAL97928.1.
RefSeqiYP_001032640.1. NC_009004.1.

Genome annotation databases

EnsemblBacteriaiCAL97928; CAL97928; llmg_1337.
GeneIDi4797323.
KEGGillm:llmg_1337.
PATRICi22283849. VBILacLac4574_1380.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY156932 Genomic DNA. Translation: AAN64306.1 .
AM406671 Genomic DNA. Translation: CAL97928.1 .
RefSeqi YP_001032640.1. NC_009004.1.

3D structure databases

ProteinModelPortali Q8GJP4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 416870.llmg_1337.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL97928 ; CAL97928 ; llmg_1337 .
GeneIDi 4797323.
KEGGi llm:llmg_1337.
PATRICi 22283849. VBILacLac4574_1380.

Phylogenomic databases

eggNOGi COG0801.
HOGENOMi HOG000022731.
KOi K00950.
K01495.
OMAi FIHEVEL.
OrthoDBi EOG6XHC8G.

Enzyme and pathway databases

UniPathwayi UPA00077 ; UER00155 .
UPA00848 ; UER00151 .
BioCyci LLAC416870:GCDT-1361-MONOMER.

Family and domain databases

Gene3Di 3.30.70.560. 1 hit.
HAMAPi MF_00223. FolE.
InterProi IPR001474. GTP_CycHdrlase_I.
IPR018234. GTP_CycHdrlase_I_CS.
IPR020602. GTP_CycHdrlase_I_dom.
IPR000550. Hppk.
[Graphical view ]
PANTHERi PTHR11109. PTHR11109. 1 hit.
Pfami PF01227. GTP_cyclohydroI. 1 hit.
PF01288. HPPK. 1 hit.
[Graphical view ]
SUPFAMi SSF55083. SSF55083. 1 hit.
TIGRFAMsi TIGR00063. folE. 1 hit.
TIGR01498. folK. 1 hit.
PROSITEi PS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
PS00794. HPPK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Increased production of folate by metabolic engineering of Lactococcus lactis."
    Sybesma W., Starrenburg M., Kleerebezem M., Mierau I., de Vos W.M., Hugenholtz J.
    Appl. Environ. Microbiol. 69:3069-3076(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363."
    Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J.
    J. Bacteriol. 189:3256-3270(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MG1363.

Entry informationi

Entry nameiFOLKE_LACLM
AccessioniPrimary (citable) accession number: Q8GJP4
Secondary accession number(s): A2RKW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: March 1, 2003
Last modified: October 1, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3