Bifunctional protein folKE - Lactococcus lactis subsp. cremoris (strain MG1363)

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Reviewed, UniProtKB/Swiss-Prot Q8GJP4 (FOLKE_LACLM)

Last modified November 3, 2009. Version 46. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Bifunctional protein folKE
Including the following 2 domains:
    1- Recommended name:
            2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase
              EC=2.7.6.3
        Alternative name(s):
            7,8 dihydro-6-hydroxymethylpterin-pyrophosphokinase
              Short name=HPPK
            6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
              Short name=PPPK
    2- Recommended name:
            GTP cyclohydrolase 1
              EC=3.5.4.16
        Alternative name(s):
            GTP cyclohydrolase I
              Short name=GTP-CH-I

Gene names

Name:	folKE
Synonyms:	folE
Ordered Locus Names:	llmg_1337

Organism

Lactococcus lactis subsp. cremoris (strain MG1363) [Complete proteome] [HAMAP]

Taxonomic identifier

416870 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

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Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

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General annotation (Comments)

Catalytic activity	ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine = AMP + (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate. HAMAP MF_00223 GTP + H₂O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate. HAMAP MF_00223
Pathway	Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1. HAMAP MF_00223 Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 4/4. HAMAP MF_00223
Subunit structure	Homopolymer By similarity.
Sequence similarities	In the N-terminal section; belongs to the HPPK family. In the C-terminal section; belongs to the GTP cyclohydrolase I family.

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Ontologies

Keywords
Biological process	Folate biosynthesis One-carbon metabolism
Ligand	ATP-binding GTP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	folic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP tetrahydrofolate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP cyclohydrolase I activity Inferred from electronic annotation. Source: HAMAP kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 349

349

Bifunctional protein folKE HAMAP MF_00223

PRO_0000119472

Regions

Region

1 – 226

226

2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase HAMAP MF_00223

Region

226 – 349

124

GTP cyclohydrolase 1 HAMAP MF_00223

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Sequences

Sequence

Length

Mass (Da)

Tools

Q8GJP4-1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 105B058C80E3A8B4
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FASTA

349

40,261

        10         20         30         40         50         60 
MQTTYLSMGS NIGDRQYYLH EAIRLLGKHP KIMIEKVSNF YESSPVGGVK QDDFTNLALK 

        70         80         90        100        110        120 
VATLLEPLEL LDFIHEVELS LNRERKIHWG PRTIDIDIIF YGNSEIQEEN LIVPHKEAFN 

       130        140        150        160        170        180 
RLFVLKPIFE LLSNDFKYYE PIREAIAKLS ESEQELHVIE EEKSPKNRIE EAVKEILFAV 

       190        200        210        220        230        240 
GENPNREGLL ETPARVAKMY EEILSSQRLT NFNEYKLFEI DSSKNDSIVL IKDIPFYSMC 

       250        260        270        280        290        300 
EHHMLPFFGK AHVAYIPDGG RIIGLSKIPR LVNYVSRKLS VQENITHDIA DILTDILKPK 

       310        320        330        340 
GVAVLVEGRH MCVEMRGVKK VNSLTKTSYF LGEFKENGEK RMEFLESLL

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Increased production of folate by metabolic engineering of Lactococcus lactis." Sybesma W., Starrenburg M., Kleerebezem M., Mierau I., de Vos W.M., Hugenholtz J. Appl. Environ. Microbiol. 69:3069-3076(2003) [PubMed: 12788700] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363." Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J. J. Bacteriol. 189:3256-3270(2007) [PubMed: 17307855] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases
	AY156932 Genomic DNA. Translation: AAN64306.1. AM406671 Genomic DNA. Translation: CAL97928.1.
RefSeq	YP_001032640.1.
3D structure databases
HSSP	HSSP built from PDB template 1FB1 based on UniProtKB P30793.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8GJP4.
Genome annotation databases
GeneID	4797323.
GenomeReviews	Gene locus llmg_1337 in contig AM406671_GR.
KEGG	llm:llmg_1337.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VEMRGVK.
Family and domain databases
HAMAP	MF_00223. Fused. [Tree]
InterPro	IPR001474. GTP_CycHdrlase_I. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. IPR018234. GTP_CycHdrlase_I_CS. IPR000550. Hppk. [Graphical view]
Gene3D	G3DSA:3.30.70.560. Hppk. 1 hit.
PANTHER	PTHR11109. GTP_cyclohydro_I. 1 hit.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. PF01288. HPPK. 1 hit. [Graphical view]
ProDom	PD003330. GTP_cyclohydroI. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00063. folE. 1 hit. TIGR01498. folK. 1 hit.
PROSITE	PS00859. GTP_CYCLOHYDROL_1_1. 1 hit. PS00860. GTP_CYCLOHYDROL_1_2. False negative. PS00794. HPPK. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

FOLKE_LACLM

Accession

Primary (citable) accession number: Q8GJP4
Secondary accession number(s): A2RKW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents