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Q8GJP4

- FOLKE_LACLM

UniProt

Q8GJP4 - FOLKE_LACLM

Protein

Bifunctional protein FolKE

Gene

folKE

Organism
Lactococcus lactis subsp. cremoris (strain MG1363)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine = AMP + (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate.
    GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate.

    Pathwayi

    GO - Molecular functioni

    1. 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. GTP binding Source: UniProtKB-KW
    4. GTP cyclohydrolase I activity Source: UniProtKB-HAMAP
    5. kinase activity Source: UniProtKB-KW
    6. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 7,8-dihydroneopterin 3'-triphosphate biosynthetic process Source: UniProtKB-UniPathway
    2. folic acid biosynthetic process Source: UniProtKB-KW
    3. one-carbon metabolic process Source: UniProtKB-HAMAP
    4. tetrahydrofolate biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Kinase, Transferase

    Keywords - Biological processi

    Folate biosynthesis, One-carbon metabolism

    Keywords - Ligandi

    ATP-binding, GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciLLAC416870:GCDT-1361-MONOMER.
    UniPathwayiUPA00077; UER00155.
    UPA00848; UER00151.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional protein FolKE
    Including the following 2 domains:
    2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (EC:2.7.6.3)
    Alternative name(s):
    6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
    Short name:
    PPPK
    7,8 dihydro-6-hydroxymethylpterin-pyrophosphokinase
    Short name:
    HPPK
    GTP cyclohydrolase 1 (EC:3.5.4.16)
    Alternative name(s):
    GTP cyclohydrolase I
    Short name:
    GTP-CH-I
    Gene namesi
    Name:folKE
    Synonyms:folE
    Ordered Locus Names:llmg_1337
    OrganismiLactococcus lactis subsp. cremoris (strain MG1363)
    Taxonomic identifieri416870 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
    ProteomesiUP000000364: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 349349Bifunctional protein FolKEPRO_0000119472Add
    BLAST

    Interactioni

    Subunit structurei

    Homopolymer.By similarity

    Protein-protein interaction databases

    STRINGi416870.llmg_1337.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8GJP4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 2262262-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinaseAdd
    BLAST
    Regioni226 – 349124GTP cyclohydrolase 1Add
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the HPPK family.Curated
    In the C-terminal section; belongs to the GTP cyclohydrolase I family.Curated

    Phylogenomic databases

    eggNOGiCOG0801.
    HOGENOMiHOG000022731.
    KOiK00950.
    K01495.
    OMAiFIHEVEL.
    OrthoDBiEOG6XHC8G.

    Family and domain databases

    Gene3Di3.30.70.560. 1 hit.
    HAMAPiMF_00223. FolE.
    InterProiIPR001474. GTP_CycHdrlase_I.
    IPR018234. GTP_CycHdrlase_I_CS.
    IPR020602. GTP_CycHdrlase_I_dom.
    IPR000550. Hppk.
    [Graphical view]
    PANTHERiPTHR11109. PTHR11109. 1 hit.
    PfamiPF01227. GTP_cyclohydroI. 1 hit.
    PF01288. HPPK. 1 hit.
    [Graphical view]
    SUPFAMiSSF55083. SSF55083. 1 hit.
    TIGRFAMsiTIGR00063. folE. 1 hit.
    TIGR01498. folK. 1 hit.
    PROSITEiPS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
    PS00794. HPPK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8GJP4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQTTYLSMGS NIGDRQYYLH EAIRLLGKHP KIMIEKVSNF YESSPVGGVK    50
    QDDFTNLALK VATLLEPLEL LDFIHEVELS LNRERKIHWG PRTIDIDIIF 100
    YGNSEIQEEN LIVPHKEAFN RLFVLKPIFE LLSNDFKYYE PIREAIAKLS 150
    ESEQELHVIE EEKSPKNRIE EAVKEILFAV GENPNREGLL ETPARVAKMY 200
    EEILSSQRLT NFNEYKLFEI DSSKNDSIVL IKDIPFYSMC EHHMLPFFGK 250
    AHVAYIPDGG RIIGLSKIPR LVNYVSRKLS VQENITHDIA DILTDILKPK 300
    GVAVLVEGRH MCVEMRGVKK VNSLTKTSYF LGEFKENGEK RMEFLESLL 349
    Length:349
    Mass (Da):40,261
    Last modified:March 1, 2003 - v1
    Checksum:i105B058C80E3A8B4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY156932 Genomic DNA. Translation: AAN64306.1.
    AM406671 Genomic DNA. Translation: CAL97928.1.
    RefSeqiYP_001032640.1. NC_009004.1.

    Genome annotation databases

    EnsemblBacteriaiCAL97928; CAL97928; llmg_1337.
    GeneIDi4797323.
    KEGGillm:llmg_1337.
    PATRICi22283849. VBILacLac4574_1380.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY156932 Genomic DNA. Translation: AAN64306.1 .
    AM406671 Genomic DNA. Translation: CAL97928.1 .
    RefSeqi YP_001032640.1. NC_009004.1.

    3D structure databases

    ProteinModelPortali Q8GJP4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 416870.llmg_1337.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAL97928 ; CAL97928 ; llmg_1337 .
    GeneIDi 4797323.
    KEGGi llm:llmg_1337.
    PATRICi 22283849. VBILacLac4574_1380.

    Phylogenomic databases

    eggNOGi COG0801.
    HOGENOMi HOG000022731.
    KOi K00950.
    K01495.
    OMAi FIHEVEL.
    OrthoDBi EOG6XHC8G.

    Enzyme and pathway databases

    UniPathwayi UPA00077 ; UER00155 .
    UPA00848 ; UER00151 .
    BioCyci LLAC416870:GCDT-1361-MONOMER.

    Family and domain databases

    Gene3Di 3.30.70.560. 1 hit.
    HAMAPi MF_00223. FolE.
    InterProi IPR001474. GTP_CycHdrlase_I.
    IPR018234. GTP_CycHdrlase_I_CS.
    IPR020602. GTP_CycHdrlase_I_dom.
    IPR000550. Hppk.
    [Graphical view ]
    PANTHERi PTHR11109. PTHR11109. 1 hit.
    Pfami PF01227. GTP_cyclohydroI. 1 hit.
    PF01288. HPPK. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55083. SSF55083. 1 hit.
    TIGRFAMsi TIGR00063. folE. 1 hit.
    TIGR01498. folK. 1 hit.
    PROSITEi PS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
    PS00794. HPPK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Increased production of folate by metabolic engineering of Lactococcus lactis."
      Sybesma W., Starrenburg M., Kleerebezem M., Mierau I., de Vos W.M., Hugenholtz J.
      Appl. Environ. Microbiol. 69:3069-3076(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363."
      Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J.
      J. Bacteriol. 189:3256-3270(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MG1363.

    Entry informationi

    Entry nameiFOLKE_LACLM
    AccessioniPrimary (citable) accession number: Q8GJP4
    Secondary accession number(s): A2RKW2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 16, 2004
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3