Q8GJM0 (HIS4_SYNE7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
EC=5.3.1.16

Alternative name(s):
Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Gene names

Name:	hisA
Ordered Locus Names:	Synpcc7942_1829
ORF Names:	sen0020

Organism

Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) [Complete proteome] [HAMAP]

Taxonomic identifier

1140 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechococcus › Synechococcus elongatus

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxyribulos-1-ylamino)methylideneamino)-1-(5-phosphoribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_01014
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9. HAMAP-Rule MF_01014
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the HisA/HisF family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	histidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase HAMAP-Rule MF_01014		PRO_0000142064
Sites
	Active site	8	1	Proton acceptor By similarity
	Active site	129	1	Proton donor By similarity
Experimental info
	Sequence conflict	138 – 145	8	RGWLEDSG → GAAGRFW in AAN46174. Ref.1
	Sequence conflict	152 – 175	24	AQQMA…DGTLQ → HSRWQTWRLRTDLHRHWARW HAS in AAN46174. Ref.1
	Sequence conflict	225	1	L → F in AAN46174. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8GJM0 [UniParc]. Last modified April 18, 2006. Version 2. Checksum: 368F941D8BA49AA7	FASTA	256	26,326
	10 20 30 40 50 60 MDVIPAIDLL GGQCVRLFQG DYDQAEVYGK DPVGMALRWA EAGAQRLHLV DLDGAKEGSP 70 80 90 100 110 120 VNAEAIATIA QRLSIPVQVG GGLRDRDTVA RLLDSGVERA ILGTVAVERP ALVEALAGEF 130 140 150 160 170 180 PGQIAVGIDA RSGKVATRGW LEDSGLTAVA LAQQMADLGA CALICTDIGR DGTLQGPNLE 190 200 210 220 230 240 ELRAIAAAVS IPVIASGGVG SLTDLLSLLP LEAQGVSGVI VGKALYTGAV DLQEALRAIG 250 SGRWQDVAVD DSSRLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Synechococcus elongatus PCC7942 cosmid 4G8." Holtman C.K., Sandoval P., Chen Y., Socias T., McMurtry S., Gonzalez A., Salinas I., Golden S.S., Youderian P. Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7942.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY157498 Genomic DNA. Translation: AAN46174.1. CP000100 Genomic DNA. Translation: ABB57859.1.
RefSeq	YP_400846.1. NC_007604.1.
3D structure databases
ProteinModelPortal	Q8GJM0.
ModBase	Search...
Protein-protein interaction databases
STRING	1140.Synpcc7942_1829.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABB57859; ABB57859; Synpcc7942_1829.
GeneID	3774404.
KEGG	syf:Synpcc7942_1829.
PATRIC	23789089. VBISynElo51371_2068.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0106.
HOGENOM	HOG000224614.
KO	K01814.
ProtClustDB	PRK00748.
Enzyme and pathway databases
BioCyc	SELO1140:GJWQ-1857-MONOMER.
UniPathway	UPA00031; UER00009.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01014. HisA.
InterPro	IPR013785. Aldolase_TIM. IPR006062. His_biosynth. IPR006063. HisA. IPR023016. Isoase_HisA. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Pfam	PF00977. His_biosynth. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00007. TIGR00007. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HIS4_SYNE7

Accession

Primary (citable) accession number: Q8GJM0
Secondary accession number(s): Q31M60

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2003
Last sequence update:	April 18, 2006
Last modified:	May 1, 2013
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program