ID   ARSC_BACME              Reviewed;         140 AA.
AC   Q8GJ74;
DT   10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   16-JUN-2009, entry version 30.
DE   RecName: Full=Protein arsC;
DE   AltName: Full=Arsenate reductase;
DE            EC=1.20.4.-;
DE   AltName: Full=Arsenical pump modifier;
DE   AltName: Full=Low molecular weight protein-tyrosine-phosphatase;
DE            EC=3.1.3.48;
GN   Name=arsC;
OS   Bacillus megaterium.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1404;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DSM 319;
RX   PubMed=15758224; DOI=10.1099/mic.0.27626-0;
RA   Nahrstedt H., Schroder C., Meinhardt F.;
RT   "Evidence for two recA genes mediating DNA repair in Bacillus
RT   megaterium.";
RL   Microbiology 151:775-787(2005).
CC   -!- FUNCTION: Reduces arsenate [As(V)] to arsenite [As(III)] and
CC       dephosphorylates tyrosine phosphorylated proteins, low-MW aryl
CC       phosphates and natural and synthetic acyl phosphates. Could switch
CC       between different functions in different circumstances (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: Protein tyrosine phosphate + H(2)O = protein
CC       tyrosine + phosphate.
CC   -!- CATALYTIC ACTIVITY: Arsenate + thioredoxin = arsenite +
CC       thioredoxin disulfide + H(2)O.
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SIMILARITY: Belongs to the low molecular weight phosphotyrosine
CC       protein phosphatase superfamily. ArsC family.
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DR   EMBL; AJ515540; CAD56680.1; -; Genomic_DNA.
DR   HSSP; P45947; 1JL3.
DR   SMR; Q8GJ74; 4-140.
DR   BRENDA; 3.1.3.48; 325.
DR   GO; GO:0030612; F:arsenate reductase (thioredoxin) activity; IEA:HAMAP.
DR   GO; GO:0004725; F:protein tyrosine phosphatase activity; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006470; P:protein amino acid dephosphorylation; IEA:InterPro.
DR   GO; GO:0046685; P:response to arsenic; IEA:UniProtKB-KW.
DR   HAMAP; MF_01624; -; 1.
DR   InterPro; IPR014064; Arsenate_reductase_StaphA.
DR   InterPro; IPR017867; Tyr_phospatase_low_mol_wt.
DR   PANTHER; PTHR11717; Low_mwt_PTPase; 1.
DR   Pfam; PF01451; LMWPc; 1.
DR   SMART; SM00226; LMWPc; 1.
DR   TIGRFAMs; TIGR02691; arsC_pI258_fam; 1.
PE   3: Inferred from homology;
KW   Arsenical resistance; Disulfide bond; Hydrolase; Oxidoreductase;
KW   Redox-active center.
FT   CHAIN         1    140       Protein arsC.
FT                                /FTId=PRO_0000162519.
FT   ACT_SITE     10     10       Nucleophile; for reductase activity and
FT                                phosphatase activity (By similarity).
FT   ACT_SITE     83     83       Nucleophile; for reductase activity (By
FT                                similarity).
FT   ACT_SITE     90     90       Nucleophile; for reductase activity (By
FT                                similarity).
FT   DISULFID     10     83       Redox-active; alternate (By similarity).
FT   DISULFID     83     90       Redox-active; alternate (By similarity).
SQ   SEQUENCE   140 AA;  15688 MW;  046DD5DB132DDCA9 CRC64;
     MSKKTLYFLC TGNSCRSQMA EGWAKKYLNN NEWDVRSAGL EAHGLNPNAV KAMKEAGVDI
     SNQTSDVIDP EILNNADLVV TLCGHAADHC PVTPPHVKRE HWGFDDPAKA EGTDEEKWAF
     FQRVRDEIAE RIQRFAETGK
//