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Protein

Protein ArsC

Gene

arsC

Organism
Bacillus megaterium (strain DSM 319)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Reduces arsenate [As(V)] to arsenite [As(III)] and dephosphorylates tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Could switch between different functions in different circumstances.UniRule annotation

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.UniRule annotation
Arsenate + thioredoxin = arsenite + thioredoxin disulfide + H2O.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei10 – 101Nucleophile; for reductase activity and phosphatase activityUniRule annotation
Active sitei83 – 831Nucleophile; for reductase activityUniRule annotation
Active sitei90 – 901Nucleophile; for reductase activityUniRule annotation

GO - Molecular functioni

  1. arsenate reductase (thioredoxin) activity Source: UniProtKB-HAMAP
  2. protein tyrosine phosphatase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. response to arsenic-containing substance Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Oxidoreductase

Keywords - Biological processi

Arsenical resistance

Enzyme and pathway databases

BioCyciBMEG592022:GIVX-1727-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein ArsCUniRule annotation
Alternative name(s):
Arsenate reductaseUniRule annotation (EC:1.20.4.-UniRule annotation)
Arsenical pump modifierUniRule annotation
Low molecular weight protein-tyrosine-phosphataseUniRule annotation (EC:3.1.3.48UniRule annotation)
Gene namesi
Name:arsCUniRule annotation
Ordered Locus Names:BMD_1727
OrganismiBacillus megaterium (strain DSM 319)
Taxonomic identifieri592022 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000002365 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 140140Protein ArsCPRO_0000162519Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi10 ↔ 83Redox-active; alternateUniRule annotation
Disulfide bondi83 ↔ 90Redox-active; alternateUniRule annotation

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ8GJ74.
SMRiQ8GJ74. Positions 1-140.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the low molecular weight phosphotyrosine protein phosphatase superfamily. ArsC family.UniRule annotation

Keywords - Domaini

Redox-active center

Phylogenomic databases

HOGENOMiHOG000273093.
KOiK03741.
OMAiQTSDMID.

Family and domain databases

HAMAPiMF_01624. Arsenate_reduct.
InterProiIPR014064. Arsenate_reductase_ArsC.
IPR023485. Ptyr_pPase_SF.
IPR017867. Tyr_phospatase_low_mol_wt.
[Graphical view]
PANTHERiPTHR11717:SF18. PTHR11717:SF18. 1 hit.
PfamiPF01451. LMWPc. 1 hit.
[Graphical view]
SMARTiSM00226. LMWPc. 1 hit.
[Graphical view]
SUPFAMiSSF52788. SSF52788. 1 hit.
TIGRFAMsiTIGR02691. arsC_pI258_fam. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8GJ74-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKKTLYFLC TGNSCRSQMA EGWAKKYLNN NEWDVRSAGL EAHGLNPNAV
60 70 80 90 100
KAMKEAGVDI SNQTSDVIDP EILNNADLVV TLCGHAADHC PVTPPHVKRE
110 120 130 140
HWGFDDPAKA EGTDEEKWAF FQRVRDEIAE RIQRFAETGK
Length:140
Mass (Da):15,688
Last modified:February 28, 2003 - v1
Checksum:i046DD5DB132DDCA9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ515540 Genomic DNA. Translation: CAD56680.1.
CP001982 Genomic DNA. Translation: ADF38582.1.
RefSeqiYP_003596932.1. NC_014103.1.

Genome annotation databases

EnsemblBacteriaiADF38582; ADF38582; BMD_1727.
GeneIDi9117118.
KEGGibmd:BMD_1727.
PATRICi37253484. VBIBacMeg104484_1668.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ515540 Genomic DNA. Translation: CAD56680.1.
CP001982 Genomic DNA. Translation: ADF38582.1.
RefSeqiYP_003596932.1. NC_014103.1.

3D structure databases

ProteinModelPortaliQ8GJ74.
SMRiQ8GJ74. Positions 1-140.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADF38582; ADF38582; BMD_1727.
GeneIDi9117118.
KEGGibmd:BMD_1727.
PATRICi37253484. VBIBacMeg104484_1668.

Phylogenomic databases

HOGENOMiHOG000273093.
KOiK03741.
OMAiQTSDMID.

Enzyme and pathway databases

BioCyciBMEG592022:GIVX-1727-MONOMER.

Family and domain databases

HAMAPiMF_01624. Arsenate_reduct.
InterProiIPR014064. Arsenate_reductase_ArsC.
IPR023485. Ptyr_pPase_SF.
IPR017867. Tyr_phospatase_low_mol_wt.
[Graphical view]
PANTHERiPTHR11717:SF18. PTHR11717:SF18. 1 hit.
PfamiPF01451. LMWPc. 1 hit.
[Graphical view]
SMARTiSM00226. LMWPc. 1 hit.
[Graphical view]
SUPFAMiSSF52788. SSF52788. 1 hit.
TIGRFAMsiTIGR02691. arsC_pI258_fam. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Evidence for two recA genes mediating DNA repair in Bacillus megaterium."
    Nahrstedt H., Schroder C., Meinhardt F.
    Microbiology 151:775-787(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genome sequences of the industrial vitamin B12-producers B. megaterium QM B1551 and DSM319 reveal new insights into the Bacillus genome evolution and pan-genome structure."
    Eppinger M., Bunk B., Johns M.A., Edirisinghe J.N., Kutumbaka K.K., Riley D.R., Creasy H.H., Koenig S.S.K., Galens K., Orvis J., Creasy T., Biedendieck R., Braun C., Grayburn S., Jahn D., Ravel J., Vary P.S.
    Submitted (JAN-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 319.

Entry informationi

Entry nameiARSC_BACMD
AccessioniPrimary (citable) accession number: Q8GJ74
Secondary accession number(s): D5DDN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2006
Last sequence update: February 28, 2003
Last modified: March 31, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.