Reviewed, UniProtKB/Swiss-Prot Q8GJ74 (ARSC_BACME)

Names and origin

Protein names

Recommended name:
    Protein arsC
Alternative name(s):
    Arsenate reductase
    EC=1.20.4.-
    Arsenical pump modifier
    Low molecular weight protein-tyrosine-phosphatase
    EC=3.1.3.48

Gene names

Name:

arsC

Organism

Bacillus megaterium

Taxonomic identifier

1404 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	140 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduces arsenate [As(V)] to arsenite [As(III)] and dephosphorylates tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Could switch between different functions in different circumstances By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. HAMAP MF_01624 Arsenate + thioredoxin = arsenite + thioredoxin disulfide + H2O. HAMAP MF_01624
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the low molecular weight phosphotyrosine protein phosphatase superfamily. ArsC family.

Ontologies

Keywords
Biological process	Arsenical resistance
Domain	Redox-active center
Molecular function	Hydrolase Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid dephosphorylation Inferred from electronic annotation. Source: InterPro response to arsenic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	arsenate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP protein tyrosine phosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 140	140	Protein arsC HAMAP MF_01624		PRO_0000162519
Sites
	Active site	10	1	Nucleophile; for reductase activity and phosphatase activity By similarity
	Active site	83	1	Nucleophile; for reductase activity By similarity
	Active site	90	1	Nucleophile; for reductase activity By similarity
Amino acid modifications
	Disulfide bond	10 ↔ 83		Redox-active; alternate By similarity
	Disulfide bond	83 ↔ 90		Redox-active; alternate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8GJ74-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 046DD5DB132DDCA9	FASTA	140	15,688
	10 20 30 40 50 60 MSKKTLYFLC TGNSCRSQMA EGWAKKYLNN NEWDVRSAGL EAHGLNPNAV KAMKEAGVDI 70 80 90 100 110 120 SNQTSDVIDP EILNNADLVV TLCGHAADHC PVTPPHVKRE HWGFDDPAKA EGTDEEKWAF 130 140 FQRVRDEIAE RIQRFAETGK

References

[1]	"Evidence for two recA genes mediating DNA repair in Bacillus megaterium." Nahrstedt H., Schroder C., Meinhardt F. Microbiology 151:775-787(2005) [PubMed: 15758224] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 319.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ515540 Genomic DNA. Translation: CAD56680.1.
3D structure databases
HSSP	HSSP built from PDB template 1JL3 based on UniProtKB P45947.
SMR	Q8GJ74. Positions 4-140.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.3.48. 325.
Family and domain databases
HAMAP	MF_01624. [Tree]
InterPro	IPR014064. Arsenate_reductase_StaphA. IPR017867. Tyr_phospatase_low_mol_wt. [Graphical view]
PANTHER	PTHR11717. Low_mwt_PTPase. 1 hit.
Pfam	PF01451. LMWPc. 1 hit. [Graphical view]
SMART	SM00226. LMWPc. 1 hit. [Graphical view]
TIGRFAMs	TIGR02691. arsC_pI258_fam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARSC_BACME

Accession

Primary (citable) accession number: Q8GJ74

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)