Q8GJ74 (ARSC_BACMD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein ArsC

Alternative name(s):
Arsenate reductase
EC=1.20.4.-
Arsenical pump modifier
Low molecular weight protein-tyrosine-phosphatase
EC=3.1.3.48

Gene names

Name:	arsC
Ordered Locus Names:	BMD_1727

Organism

Bacillus megaterium (strain DSM 319) [Complete proteome] [HAMAP]

Taxonomic identifier

592022 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus megaterium

Protein attributes

Sequence length	140 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reduces arsenate [As(V)] to arsenite [As(III)] and dephosphorylates tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Could switch between different functions in different circumstances By similarity. HAMAP-Rule MF_01624
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. HAMAP-Rule MF_01624 Arsenate + thioredoxin = arsenite + thioredoxin disulfide + H2O. HAMAP-Rule MF_01624
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the low molecular weight phosphotyrosine protein phosphatase superfamily. ArsC family.

Ontologies

Keywords
Biological process	Arsenical resistance
Domain	Redox-active center
Molecular function	Hydrolase Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC response to arsenic-containing substance Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	arsenate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP protein tyrosine phosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 140	140	Protein ArsC HAMAP-Rule MF_01624		PRO_0000162519
Sites
	Active site	10	1	Nucleophile; for reductase activity and phosphatase activity By similarity
	Active site	83	1	Nucleophile; for reductase activity By similarity
	Active site	90	1	Nucleophile; for reductase activity By similarity
Amino acid modifications
	Disulfide bond	10 ↔ 83		Redox-active; alternate By similarity
	Disulfide bond	83 ↔ 90		Redox-active; alternate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8GJ74 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 046DD5DB132DDCA9	FASTA	140	15,688
	10 20 30 40 50 60 MSKKTLYFLC TGNSCRSQMA EGWAKKYLNN NEWDVRSAGL EAHGLNPNAV KAMKEAGVDI 70 80 90 100 110 120 SNQTSDVIDP EILNNADLVV TLCGHAADHC PVTPPHVKRE HWGFDDPAKA EGTDEEKWAF 130 140 FQRVRDEIAE RIQRFAETGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence for two recA genes mediating DNA repair in Bacillus megaterium." Nahrstedt H., Schroder C., Meinhardt F. Microbiology 151:775-787(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome sequences of the industrial vitamin B12-producers B. megaterium QM B1551 and DSM319 reveal new insights into the Bacillus genome evolution and pan-genome structure." Eppinger M., Bunk B., Johns M.A., Edirisinghe J.N., Kutumbaka K.K., Riley D.R., Creasy H.H., Koenig S.S.K., Galens K., Orvis J., Creasy T., Biedendieck R., Braun C., Grayburn S., Jahn D., Ravel J., Vary P.S. Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 319.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ515540 Genomic DNA. Translation: CAD56680.1. CP001982 Genomic DNA. Translation: ADF38582.1.
RefSeq	YP_003596932.1. NC_014103.1.
3D structure databases
ProteinModelPortal	Q8GJ74.
SMR	Q8GJ74. Positions 1-140.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADF38582; ADF38582; BMD_1727.
GeneID	9117118.
KEGG	bmd:BMD_1727.
PATRIC	37253484. VBIBacMeg104484_1668.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000273093.
KO	K03741.
ProtClustDB	CLSK2839638.
Enzyme and pathway databases
BioCyc	BMEG592022:GIVX-1727-MONOMER.
Family and domain databases
HAMAP	MF_01624. Arsenate_reduct.
InterPro	IPR014064. Arsenate_reductase_ArsC. IPR023485. Ptyr_pPase_SF. IPR017867. Tyr_phospatase_low_mol_wt. [Graphical view]
PANTHER	PTHR11717. PTHR11717. 1 hit.
Pfam	PF01451. LMWPc. 1 hit. [Graphical view]
SMART	SM00226. LMWPc. 1 hit. [Graphical view]
SUPFAM	SSF52788. Tyr_Pase_low_mol_wt. 1 hit.
TIGRFAMs	TIGR02691. arsC_pI258_fam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARSC_BACMD

Accession

Primary (citable) accession number: Q8GJ74
Secondary accession number(s): D5DDN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program